Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Improvement of a Screening System for Environmental Mutagens by Means of a Specific Locus Mutation of the Silkworm, Bombyx mori

누에를 이용한 환경 변이원 검색계 재선

  • Yoon, Hyung-Joo (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Sam-Eun (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Kim, Jong-Gill (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA) ;
  • Choi, Ji-Young (Department of Agricultural Biology, The National Institute of Agricultural Science & Technology, RDA)
  • 윤형주 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 김삼은 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 김종길 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과) ;
  • 최지영 (농촌진흥청 농업과학기술원 농업생물부 유용곤충과)
  • Published : 2006.12.30
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Abstract

The efficiency to detect mutagenicity of the system using a specific locus mutation of Bombyx mori was examined and improved. In the system, mutagenicity could be detected by the egg colour manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. Among tested four mutagens, MMC had specially high sensitivity in the oocytes of silkworm and EMS had in the spermatozoa. PCB and dioxin showed a positive effect in both the oocytes and spermatozoa. In a consequence of sensitivity of mutagen by mating number of male moth of B. mori, treated mutagen, there was no difference between one mating - and three mating - male moth in sensitivity of mutagen. Sun3ho, B. mori variety, which showed high sensitivity to mutagens was improved in the major characteristics by crossing of C5 and N12.

누에의 가시 돌연변이 형질을 이용한 환경변이원 검색계 수립을 위하여 MMC, EMS, PCB에 대한 누에알 변이원 검색계의 감수성을 조사한 결과, 난모세포계는 MMC, 정세포계는 EMS에 대하여 특정적으로 높은 감수성을 보였고, PCB와 dioxin에 대해서도 양성반응을 얻을 수 있었다. 수나방의 사정횟수에 따른 정세포의 변이원 감수성 변화를 시험한 결과, 1교와 3교 때 사정된 정자 간에는 큰 차이가 없었다. 변이원 감수성은 높으나, 산란성, 우화율 등 검색계로서 주요형질이 극히 불량한 선3호는 C5, Nl2, 한삼면과의 교잡으로 주요형질이 개선되고 높은 변이원 감수성을 유지하였다.

Keywords

References

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