• Toxicological Research
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• v.18 no.2
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• pp.149-157
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• 2002

Though the bamboo salt, called as "JUKYUM" has been widely used in Korea as panacea, it's toxicity was not screened completely. To investigate the toxicity of bamboo salt, we compared with the toxicity of crude salt and reagent-grade NaCl by performing repeated dose (3 month's) oral toxicity test in SD rats. Crude salt, natural sun-dried salt (crude salt) production, was purchased from the western seashore of Korean peninsular and reagent-grade NaCl was purchased from Sigma company. Results of repeated dose oral toxicity tests for 3 months (bamboo salt; 750, 1500, 3000 mg/kg/day, crude salt : 3000 mg/kg/day, reagent-grade NaCl; 3000 mg/kg/day) suggested that the bamboo salt treated group show no significant toxicological findings with body weights and organ weighs changes, and hematological, serum bio-chemical and histopathological findings compared with other groups.er groups.

• Bulletin of the Korean Chemical Society
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• v.31 no.9
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• pp.2557-2560
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• 2010

To assay for antioxidant capacity of natural products considered important in producing human health benefits, a practical and economical method using pellet techniques was developed. A standard visualizing reagent, 1,1diphenyl-2-picryl-hydrazyl (DPPH), was mixed with a water-miscible polyvinyl alcohol (PVA), serving as a solid phase support for the DPPH reagent. A DPPH pellet was prepared by dropping a small volume of the DPPH solution onto PET film, and drying in an oven. The PVA-based DPPH pellet was dissolved into water, in which the water-miscible PVA plays as a non-ionic surfactant to help the DPPH reagent to be dissolved into the solvent. Using the DPPH assay, the antioxidant capacity of water-soluble extracts of black soybean, barley, green tea, and green gram was examined. Among the natural products tested, green tea showed the highest antioxidant capacity. This PVA-based DPPH antioxidant assay can be further applied in the natural food, raw plant material, and health product inspection field.

• Bulletin of the Korean Chemical Society
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• v.35 no.12
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• pp.3453-3458
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• 2014

An efficient and practical synthesis of natural moracins, which have diverse range of biological properties including anticancer, antioxidant, and antibacterial activities, has been achieved using $Pd(OAc)_2/P(^tBu)_3-HBF_4$ as a Sonogashira coupling reagent which solved the unreactive problems in case of higher electron density of haloaryl compounds in the reaction. Lowering electron density of halophenol with acetylation and changing Sonogashira coupling reagent from $PdCl_2(PPh_3)_2$ to $Pd(OAc)_2/P(^tBu)_3-HBF_4$ smoothly produce the benzofuran structures in the syntheses of moracins M, N and S. The electron deficient halobenzaldehyde, however, easily forms the benzofuran using original Sonogashira conditions, and utilized for the first synthesis of moracin Y.

• YAKHAK HOEJI
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• v.37 no.3
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• pp.209-215
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• 1993

Basic drugs could be extracted as ion-paired complexes with Lumogallion, Superchrome Garnet Y and their alkyl derivatives from aqueous acid solution, and then determined fluoro-metrically after addition of aluminum ion. The analysis was carried out as follows; To a 1 ml portion of basic drugs (10$^{-9}$~2$\times$0$^{-8}$mole/ml), 1ml of 0.01w/v% fluorescent reagent solution, and 10ml of dichloroethane are added. The mixture is stirred for 1 minute. After standing for a few minutes, the dichloroethane layer is transfered to 1 ml of 0.1w/v% Al(NO$_{3}$)$_{3}$ ethanol solution. After mixing, and standing for 30 minutes at room temperature, the fluorescence intensity is measured with each maximum excitation and emission wavelength. The reagent blank is run through the whole procedure. From the degree of enhancement of fluorescence intensity, hexyl and dodecyl lumogallion and Superchrome Garnet Y were judged to be the useful one of fluorescent reagent for basic drugs analysis.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.10 no.S_4
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• pp.165-172
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• 2001

We investigated the optimal experimental conditions and reaction kinetics for the decompositions of PCE, TCE, naphthalene, and chloroform using conventional Fenton oxidation process. Additionally, the influence of pH on the decompositions of PCE was also evaluated. The results indicated that the optimal pH value was around 3. The dosage of Fenton's reagent and the molar ratio of hydrogen peroxide to ferrous ion for an approximately complete decomposition was found to depend on the properties of the organic compound. Due to their unsaturated structures, the results show that PCE, TCE, and naphthalene could be all effectively decomposed by Fenton's reagent oxidation. Their unsaturated structures could be mostly destoyed within first 1-2 minutes at a low dosage with an certain molar ratio of hydrogen peroxide to ferrous ion. However the saturated compound such as chloroform was more difficult to decompose even with a relatively high dosage of Fenton's reagent.

• BMB Reports
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• v.31 no.2
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• pp.139-143
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• 1998

The catabolic ${\alpha}-acetolactate$ synthase purified from Serratia marcescens ATCC 25419 was rapidly inactivated by the tryptophane-specific reagent, N -bromosuccinimide, and the arginine-specific reagent, phenylglyoxal. The enzyme was inactivated slowly by the cysteine-specific reagent N-ethylmaleimide. The second-order rate constants for the inactivation by N-bromosuccinimide, phenylglyoxal. and N -ethylmaleimide were $114,749M^{-1}min^{-1}$, $304.3M^{-1}min^{-1}$, and $5.1M^{-1}min^{-1}$, respectively. The reaction order with respect to N-bromosuccinimide, phenylglyoxal, and N-ethylmaleimide were 1.5,0.71, and 0.86, respectively. The inactivation of the catabolic aacetolactate synthase by these modifying reagents was protected by pyruvate. These results suggest that essential tryptophane, arginine, and cysteine residues are located at or near the active site of the catabolic ${\alpha}-acetolactate$ synthase.

• 한국생물공학회:학술대회논문집
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• 2002.04a
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• pp.165-168
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• 2002

TLC(Thin Layer Chromatography) 는 제약산업이나 생화학 연구 그리고 여러 산업 현장에서 널리 이용되는 화학 분석 방법이다 . 본 연구에서는 적은 비용으로도 많은 양의 시료를 신속하게 분리할 수 있는 TLC를 이용하여 유기산인 젖산 (Lactic acid) 을 분리는 전개방법을 개발하였다 . 전개용매는 2 가지 용매 (1) nitroethane nitromethane : ethanol: water: I-propanol = 1 : 2 : 3 : 4 : 5 (v/v/v/v/v), (2) diisopropyl ether. formic acid. water = 90 : 7 : 3 (v/v/v) 를 사용하였다 . 발색시약은 A : bromocresol purple reagent I, B . bromocresol purple reagent II, C : bromocresol green-bromophenol blue-potassium permanganate reagent 를 사용하여 분석하는 방법을 개발하였다 $^6)$. 젖산의 분리는 silica gel TLC plate를 이용하는 경우 , 용매 (1) 에 발색시약 B를 사용했을 때 , 분리 확인이 가장 좋았다.

• Journal of the East Asian Society of Dietary Life
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• v.10 no.1
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• pp.55-61
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• 2000

Physicochemical and sensory characteristics of Yukwa(fried rice cookie) made by different processing condition were investigated to estabilish the optimum expansion of Yukwa. The expansion rate and crispness of Yukwa prepared by soaking waxy rice for 24 hours was satisfactory . Addition of 15% soy slurry gave better effect on increasing the expantion rate, crispiness and sensory preference than no addition of soy slurry . Yeast, Soju(distilled liquor) , Mackeali(rice wine, turbid) and Chunju(rice wine, clear) as expansion reagent were added to Yukwa prepration . Yeast and Mackeali among tested expansion reagent showed better expansion rate and lower hardness meausred by texturometer than Chunju. Yukwa added chungju showed more fine structure and lower expantion rate than others. Microwave puffed Yukwa was less expansion rate, but same level of crispiness and hardness compared with oil puffed. And no difference in sensory characteristics such as degree of crispiness and stickiness of microwave puffed and oil puffed.

• Bulletin of the Korean Chemical Society
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• v.31 no.4
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• pp.949-952
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• 2010

A new and efficient reagent for the conversion of primary and secondary alcohols into their corresponding aldehydes and ketones is introduced. The reagent was easily prepared from the reaction of DBU with molecular bromine in $CHCl_3$. The structure of the reagent as $DBUH^+{Br_3}^-$ was determined by single crystal X-ray diffraction analysis.

• Journal of Haehwa Medicine
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• v.9 no.1
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• pp.183-192
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• 2000

1. The emotion is given before birth, and because of not perfect of human being's consciseness, individual differance exist. 2. Every emotion act first on the heart and following other organ which possess each of the five emotion. Therfore the heart plays impartant role on the emotion and emerge five organ in the oriental medicine theory. 3. The emotion was expressed by external reagent, but the more importante reagent is internal situation such as qi(氣), xie(血), yin(陰), yang(陽), according to these reagent the existing time and strength is different. 4. The emotion is different according to the weak-strong and big-small of zang-fu(臟腑), and the size of zang-fu(臟腑) is determined by emotional specificity in the si-xiang medicinal (四象醫學)theory.