• Title/Summary/Keyword: rate of strain

Search Result 3,162, Processing Time 0.038 seconds

Isolation of Biogenic Amine Non-producing Lactobacillus brevis SBB07 and Its Potential Probiotic Properties (바이오제닉 아민 비생성 Lactobacillus brevis SBB07의 분리 및 잠재적 프로바이오틱스 특성 분석)

  • Yang, Hee-Jong;Jeong, Su-Ji;Jeong, Seong-Yeop;Ryu, Myeong Seon;Jeong, Do-Youn
    • Journal of Life Science
    • /
    • v.28 no.1
    • /
    • pp.68-77
    • /
    • 2018
  • The purpose of this study was to isolate the probiotic lactic acid bacteria, and verify the possibility of the final selection strain as probiotic material. For screening of biogenic amines non-producing microorganisms, 42 lactic acid bacteria were isolated from various berries, extract and vinegar grown in Sunchang. Isolates were investigated for various physiological activities such as extracellular enzyme, antimicrobial and antioxidant activities, and 5 isolates were firstly screened. SBB07 was finally selected by analyzing the biogenic amine, and named Lactobacillus brevis SBB07 by 16S rRNA sequencing analysis. Next, SBB07 was assayed for their survival ability when exposed to acidic and bile conditions as well as heat and antibiotic resistance. As a result, SBB07 showed more than 86% and 54% higher survival rate in acidic condition at pH 2.0 and bile resistance with 0.5% oxgall. In addition, SBB07 showed a survival rate of more than 113% in $60^{\circ}C$, and also confirmed that it has resistant to various antibiotics. As a result of confirming the possibility of prebiotics, SBB07 showed the best utilization of GOS as a prebiotic substrate, and utilization of FOS and inulin were also high. These results suggest that SBB07 have good potential for application as probiotic lactic acid bacteria.

Anti-Toxoplasmosis Effect of the Herbal Extracts Plantago asiatica L. (차전초(Plantago asiatica L.)의 항톡소포자충증 효과)

  • Hong, Sunhwa;Kim, Okjin
    • Korean Journal of Plant Resources
    • /
    • v.29 no.1
    • /
    • pp.26-31
    • /
    • 2016
  • Toxoplasmosis is an important cause of foodborne, inflammatory illnesses, as well as congenital abnormalities. Currently available therapies are ineffective for persistent chronic disease and congenital toxoplasmosis or have severe side effects which may result in life-threatening complications. There is an urgent need for safe and effective therapies to eliminate or treat this cosmopolitan infectious disease. The aim of this study was to investigate the in vitro anti-Toxoplasma activities of Plantago asiatica L., one of the herbal extracts, using tachyzoit of T. gondii RH strain infected HeLa cells. As the results, the selectivity of Plantago asiatica L. extract was 4.5, which was higher than Sulfadiazine selectivity (0.4). Also, we perfomed the cell proliferation inhibition test and the morphological study to evaluate the anti-T. gondii activity of Plantago asiatica L. extract with HeLa cells. As the results, the inhibition rate of the Plantago asiatica L. extract was high inhibition rate. This indicates that the Plantago asiatica L. extract may be used for new anti-T. gondii agent.

The Effects of Dietary Chinese Cabbage Kimchi Juice on the Lipid Metabolism and Body Weight Gain in Rats Fed High-Calories-Diet (배추김치 즙 투여가 고 열량 섭취 흰쥐의 지질대사 및 체중변화에 미치는 영향)

  • 서화중;서유석
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.33 no.1
    • /
    • pp.91-100
    • /
    • 2004
  • Weanling male rats of Wistar strain were fed relatively high-calories-diet containing 1% (K$_1$ group), 5% (K$_{5}$ group) and 10% Chinese cabbage kimchi juice (K$_{10}$ group) for 4 weeks to study their effects on body weight gain rate, serum lipids levels and other blood biochemical parameters. In this study it was found out that significant decrease in body weight gain rate, diet efficiency ratio, serum triglyceride level, serum total cholesterol level, blood glucose level and LDL-cholesterol level was observed in $K_{10}$ group as compared to control group fed only high-calories-diet. Significant increase in HDL-cholesterol level, GPT activity, GOT activity and creatinine level was found in $K_{10}$ group as compared to control group. But creatinine level, GPT and GOT activity of $K_{10}$ group were within the normal ranges. The results suggest that long term intake of traditional Chinese cabbage kimchi in large dose have the lowering effect on blood lipid and blood glucose level. There is no possibility of adverse actions on renal and hepatic functions by ordinary intake of Chinese cabbage kimchi in spite of being rich in salt and hot spices such as garlic, onion, ginger and red pepper.r.r.

The Study of annealing condition and press method of glass for making glasses lens (안경렌즈 제작을 위한 유리의 열처리조건과 성형방법에 관한 연구)

  • Cha, Jung Won;Ha, Tae Wook
    • Journal of Korean Ophthalmic Optics Society
    • /
    • v.6 no.1
    • /
    • pp.133-137
    • /
    • 2001
  • The method to make glass is tried first thing in domestic study for glasses lens. Tg of glass KzFSl was measured by DSC experiment and press method of glass of meniscus shape was studied for minimize the cost. Tg was increased $483^{\circ}C$ to $501^{\circ}C$ with increasing heating rate $2^{\circ}C/min$ to $20^{\circ}C/min$. It shows Arrhenius temperature dependence and the activation energy of Tg is ${\Delta}E$=409 kJ/mole(4.25eV) by using Ozawa method and Tg is near $480^{\circ}C$. The melt of KzFSl powder was poured to concave cast stainless and pressed convex cast stainless. The quenched glass was slow cooled with cooling rate $0.25^{\circ}C/min$ in temperature range $520{\sim}430^{\circ}C$. The glass was made without strain. It has meniscus shape and same size to commercial glasses lens.

  • PDF

Environmental and Genetic Aspects of Obesity in Broilers (육계의 비만현상에 관한 고찰)

  • 김재홍
    • Korean Journal of Poultry Science
    • /
    • v.9 no.1
    • /
    • pp.1-16
    • /
    • 1982
  • Excessive fat deposition, particularly in the abdominal region, has become a problem in broiler production. When the caloric intake exceeds the body demands for energy, excess food is stored as fat in broilers. Researchers have shown that fat deposition varies with breed, strain, sex age, nutrition, exercise, ambient temperature and rearing systems. These factors affect fat deposition through their effects on the size or the number of adipose cells or a combination of both. In some measurements on live birds to predict body fat, the wet weight and percentage fat of skin in pectoral feather tract are significantly correlated with percentage abdominal fat. But these correlation coefficients are not so high. Therefore, correlation coefficients indicate that these measurements on live birds ate not useful for estimating body fat weight and percentage. Most reports show that an increase in the proportion of carcass fat, when measured at a given age, is correlated with selection for increased body weight. On the other hand some research results show that selection for body$.$weight gain dose not lead to an alteration in the proportion carcass fat when measured at a given body weight. Besides, selection for improved food conversion efficiency alone resulted in a decrease in carcass fat and an increase in protein and water when measured at either a given age or body weight, Thus eventhough it is uncertain whether carcass fat is increasing as a result of body-weight selection in broilers: however it is clear that selection for improved food conversion efficiency, either alone or in combination with growth rate, should result in leaner carcasses than selection for growth rate alone.

  • PDF

Fracture Simulation of UHPFRC Girder with the Interface Type Model (경계형 모델을 사용한 초고강도 섬유보강 콘크리트거더의 파괴역학적 해석)

  • Guo, Yi-Hong;Han, Sang-Mook
    • Journal of the Computational Structural Engineering Institute of Korea
    • /
    • v.23 no.1
    • /
    • pp.81-94
    • /
    • 2010
  • This paper deals with the fracture simulation of UHPFRC girder with the interface type model. Based on the existing numerical simulation of quasi-brittle fracture in normal strength concrete, constitutive modeling for UHPFRC I-girder has been improved by including a tensile hardening at the failure surface. The finite element formulation is based on a triangular unit, constructed from constant strain triangles, with nodes along its sides and neither at the vertex nor the center of the unit. Fracture is simulated through a hardening/softening fracture constitutive law in tension, a softening fracture constitutive law in shear as well as in compression at the boundary nodes, with the material within the triangular unit remaining linear elastic. LCP is used to formulate the path-dependent hardening-softening behavior in non-holonomic rate form and a mathematical programming algorithm is employed to solve the LCP. The piece-wise linear inelastic yielding-failure/failure surface is modeled with two compressive caps, two Mohr-Coulomb failure surfaces, a tensile yielding surface and a tensile failure surface. The comparison between test results and numerical results indicates this method effectively simulates the deformation and failure of specimen.

Effect of High Temperature Steam Oxidation on Yielding of Zircaloy-4 PWR Fuel Cladding -Expanding Copper Mandrel Test- (가압경수형 핵연료 피복관 지르칼로이-4의 항복현상에 대한 고온 수증기 산화의 영향 -구리 맨드렐 팽창시험법-)

  • Kye-Ho Nho;Sun-Pil Choi;Byong-Whi Lee
    • Nuclear Engineering and Technology
    • /
    • v.21 no.2
    • /
    • pp.111-122
    • /
    • 1989
  • With the Zircaloy-4 tube oxidized in high temperature (1323 K) steam for 5, 10, 30 and 60 minutes, the expanding copper mandrel test was carried out over a temperature range of 673-l173k at $\varepsilon\;=\;3.0\times10^5S\;^1$. The oxidation parameters $(K_i)$ in the present study were linearly proportional to square root of time $(Ki= \delta_{kit})$ and their rate constants ($\delta_{ki}$) are 0.281, 2.82, and 2.313 for weight gain and thickness of Zr02 and $\alpha$(0) layer, respectively. Activation energy for high temperature (873-1073k) plastic deformation of Zircaloy-4 increases from 251 KJ/mol to 323 KJ/mol with increase in oxidation time from 5 minutes to 60 minutes due to the high strengthened Zr02. With the oxide layer thickness [K ; expressed in "Equivalent Cladding Reacted" (ECR,%)] and the yield stress obtained from the mandrel test, an empirical relation was derived as ($\sigma/C)^n=K^mexp$ (Q/RT) with n=6.9, m=5.7, C=0.155, 0.138, 0.051, and 0.046 MPa for Q=251, 258, 316, 323 KJ/mol, respectively.

  • PDF

Experimental Studies on the Properties of Epoxy Resin Mortars (에폭시 수지 모르터의 특성에 관한 실험적 연구)

  • 연규석;강신업
    • Magazine of the Korean Society of Agricultural Engineers
    • /
    • v.26 no.1
    • /
    • pp.52-72
    • /
    • 1984
  • This study was performed to obtain the basic data which can be applied to the use of epoxy resin mortars. The data was based on the properties of epoxy resin mortars depending upon various mixing ratios to compare those of cement mortar. The resin which was used at this experiment was Epi-Bis type epoxy resin which is extensively being used as concrete structures. In the case of epoxy resin mortar, mixing ratios of resin to fine aggregate were 1: 2, 1: 4, 1: 6, 1: 8, 1:10, 1 :12 and 1:14, but the ratio of cement to fine aggregate in cement mortar was 1 : 2.5. The results obtained are summarized as follows; 1.When the mixing ratio was 1: 6, the highest density was 2.01 g/cm$^3$, being lower than 2.13 g/cm$^3$ of that of cement mortar. 2.According to the water absorption and water permeability test, the watertightness was shown very high at the mixing ratios of 1: 2, 1: 4 and 1: 6. But then the mixing ratio was less than 1 : 6, the watertightness considerably decreased. By this result, it was regarded that optimum mixing ratio of epoxy resin mortar for watertight structures should be richer mixing ratio than 1: 6. 3.The hardening shrinkage was large as the mixing ratio became leaner, but the values were remarkably small as compared with cement mortar. And the influence of dryness and moisture was exerted little at richer mixing ratio than 1: 6, but its effect was obvious at the lean mixing ratio, 1: 8, 1:10,1:12 and 1:14. It was confirmed that the optimum mixing ratio for concrete structures which would be influenced by the repeated dryness and moisture should be rich mixing ratio higher than 1: 6. 4.The compressive, bending and splitting tensile strenghs were observed very high, even the value at the mixing ratio of 1:14 was higher than that of cement mortar. It showed that epoxy resin mortar especially was to have high strength in bending and splitting tensile strength. Also, the initial strength within 24 hours gave rise to high value. Thus it was clear that epoxy resin was rapid hardening material. The multiple regression equations of strength were computed depending on a function of mixing ratios and curing times. 5.The elastic moduli derived from the compressive stress-strain curve were slightly smaller than the value of cement mortar, and the toughness of epoxy resin mortar was larger than that of cement mortar. 6.The impact resistance was strong compared with cement mortar at all mixing ratios. Especially, bending impact strength by the square pillar specimens was higher than the impact resistance of flat specimens or cylinderic specimens. 7.The Brinell hardness was relatively larger than that of cement mortar, but it gradually decreased with the decline of mixing ratio, and Brinell hardness at mixing ratio of 1 :14 was much the same as cement mortar. 8.The abrasion rate of epoxy resin mortar at all mixing ratio, when Losangeles abation testing machine revolved 500 times, was very low. Even mixing ratio of 1 :14 was no more than 31.41%, which was less than critical abrasion rate 40% of coarse aggregate for cement concrete. Consequently, the abrasion rate of epoxy resin mortar was superior to cement mortar, and the relation between abrasion rate and Brinell hardness was highly significant as exponential curve. 9.The highest bond strength of epoxy resin mortar was 12.9 kg/cm$^2$ at the mixing ratio of 1:2. The failure of bonded flat steel specimens occurred on the part of epoxy resin mortar at the mixing ratio of 1: 2 and 1: 4, and that of bonded cement concrete specimens was fond on the part of combained concrete at the mixing ratio of 1 : 2 ,1: 4 and 1: 6. It was confirmed that the optimum mixing ratio for bonding of steel plate, and of cement concrete should be rich mixing ratio above 1 : 4 and 1 : 6 respectively. 10.The variations of color tone by heating began to take place at about 60˚C, and the ultimate change occurred at 120˚C. The compressive, bending and splitting tensile strengths increased with rising temperature up to 80˚ C, but these rapidly decreased when temperature was above 800 C. Accordingly, it was evident that the resistance temperature of epoxy resin mortar was about 80˚C which was generally considered lower than that of the other concrete materials. But it is likely that there is no problem in epoxy resin mortar when used for unnecessary materials of high temperature resistance. The multiple regression equations of strength were computed depending on a function of mixing ratios and heating temperatures. 11.The susceptibility to chemical attack of cement mortar was easily affected by inorganic and organic acid. and that of epoxy resin mortar with mixing ratio of 1: 4 was of great resistance. On the other hand, when mixing ratio was lower than 1 : 8 epoxy resin mortar had very poor resistance, especially being poor resistant to organicacid. Therefore, for the structures requiring chemical resistance optimum mixing of epoxy resin mortar should be rich mixing ratio higher than 1: 4.

  • PDF

Studies on the Preserving Method for Inhibiting Feed Degradation (사료변질 방지를 위한 보존방법에 관한 연구)

  • 김대진;방극승;김영길;최경문;김진성
    • Korean Journal of Poultry Science
    • /
    • v.8 no.1
    • /
    • pp.7-14
    • /
    • 1981
  • The experiment using Anake broiler strain chicks was conducted to study the effort fungistatic agents on microbial counts, Ins of nutrient, growth rate and feed efficiency of the broiler. Feed was adjusted to 12% and 15% moisture level during hot and high humidity season and sorbic acid at the level of 0.02%, 0.04% and Ca-propionic acid at the level 0.1% 0.2% was added. The results obtained were as follows. 1, The Addition of fungistatic agents could slightly reduce mold and yeast counts. The highest effect on inhibition of mold and yeast counts was observed for the addition of sorbic acid at 0.04% level to the fled contained 15% moisture. 2, Approximately 14% starch loss of ground corn was observed from the fred contained 15% moisture and the loss could be diminished to 3-7% by the addition of fungistatic agents with the superior effect of sorbic acid to Ca-propionic acid. 3. Approximately 15% fat loss was detected when high moisture fled was und and this was reduced to 7% by the addition of 0.04% sorbic acid to the feed. 4. Significantly higher growth rate (p<0.05) during starter period was observed for low moisture feed added by sorbic acid compared with that for high moisture diet without fungistatic agents or with Ca-propionate at the level of 0.1%. 5. Significantly lower feed efficiency (p<0.05) during starter period of high moisture feed without fungistatic agents was observed; hower no significant different response was detected by either moisturer level of kinds and levels of fungistatic agents used. 6. Significantly higher growth rate (p<0.05) during finisher period of lower moisture feed with 0.04% sorbic acid was observed compared with that of high moisture fled without fungistatic agents or the feed added by 0.1% Ca-propionate level. 7. Significantly higher feed efficiency (p<0.05) during finisher period of low moisture feed added by sorbic acid 0.02% or 0.04% level was found compared with that of high moisture feed without fungistatic agents and low moisture feed added by Ca-propionate at the level of 0.1% or 0.2%. 8. Mort bacteria, mold and yeast were observed in the ceca than in small intestine. The. moisture content of dict had no effect on intestinal microfloral counts. However, numbers of mold and yeast of intestine could slightly be reduced by fungistatic agents administration. 9. Nothing but encephalomalacia to chicks fed feed contained 15% moisture without addition of fungistatic agents was observed. In conclusion, addition of either sorbid acid at 0.04% level and Ca-propionate at 0.2% level to high moisture feed or reduced moisture level to 12% could be con-sidered more effective to enhance growth rate and fled efficiency of broilers during summer period.

  • PDF

Simple Method to Discriminate the Fungicide Resistant Botrytis cinerea Strain in Tomatoes (토마토 잿빛곰팡이병균 약제저항성 간이 판별법)

  • Lee, Mun Haeng;Lee, Hee Kyoung;Kim, Sung Eun;Lee, Hwan Gu;Lee, Sun Gye;Yu, Seung Hun;Kim, Young Shik;Kim, Sang Woo;Lee, Youn Su
    • The Korean Journal of Mycology
    • /
    • v.41 no.3
    • /
    • pp.172-180
    • /
    • 2013
  • Grey mold infection rate in tomato was investigated with the inoculation of dead flowers on Botrytis selective media. The grey mold infection rate of flower after fruiting were higher in the order of after 45 days, after 25 days, and fruiting day with 100%, 87% and 65%, respectively. The number of infected flowers were increased with time increase after the flowering before fruiting. BSM (Botrytis selective medium) was used to check grey mold infection rate depending on the flowering stage and cultivar. Grey mold infection rate depending on the flowering stage was similar in all the beef-tomato cultivar as 1.5~5% at preflowering, 1.5~45% at flowering and 75~90% at fruiting. On the other hand, cherry tomato cultivar "KoKo" had lower infection rates of 0~3.5% at pre-flowering, 10~30% at flowering and 20~50% at fruiting. These resulted from the fact that beaf-tomato cultivar have much bigger flowers and larger amount of pollens compared to those of cherry tomato cultivar. The amounts of falling pollens of Botrytis spp. were checked for beaf-tomato cultivar and cherry tomato cultivar using BSTM. The amounts of falling pollens were increased as growth period was extended, and the amount of spores increased rapidly during the outbreak of grey mold. Twelve field trials in Buyeo and Iksan areas showed that Fluazinam, and Diethofencarb+Carbendazim were effective fungicides to control tomato grey mold, and these results were similar to those of field trials with BSTM. This is the first report of Fluazinam as a effective fungicide for the control of grey mold of tomato even though it has not been registered yet for the control of gray mold in tomato.