Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Isolation of Biogenic Amine Non-producing Lactobacillus brevis SBB07 and Its Potential Probiotic Properties

바이오제닉 아민 비생성 Lactobacillus brevis SBB07의 분리 및 잠재적 프로바이오틱스 특성 분석

  • 양희종 ((재)발효미생물산업진흥원) ;
  • 정수지 ((재)발효미생물산업진흥원) ;
  • 정성엽 ((재)발효미생물산업진흥원) ;
  • 류명선 ((재)발효미생물산업진흥원) ;
  • 정도연 ((재)발효미생물산업진흥원)
  • Received : 2017.10.13
  • Accepted : 2018.01.18
  • Published : 2018.01.30
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Abstract

The purpose of this study was to isolate the probiotic lactic acid bacteria, and verify the possibility of the final selection strain as probiotic material. For screening of biogenic amines non-producing microorganisms, 42 lactic acid bacteria were isolated from various berries, extract and vinegar grown in Sunchang. Isolates were investigated for various physiological activities such as extracellular enzyme, antimicrobial and antioxidant activities, and 5 isolates were firstly screened. SBB07 was finally selected by analyzing the biogenic amine, and named Lactobacillus brevis SBB07 by 16S rRNA sequencing analysis. Next, SBB07 was assayed for their survival ability when exposed to acidic and bile conditions as well as heat and antibiotic resistance. As a result, SBB07 showed more than 86% and 54% higher survival rate in acidic condition at pH 2.0 and bile resistance with 0.5% oxgall. In addition, SBB07 showed a survival rate of more than 113% in $60^{\circ}C$, and also confirmed that it has resistant to various antibiotics. As a result of confirming the possibility of prebiotics, SBB07 showed the best utilization of GOS as a prebiotic substrate, and utilization of FOS and inulin were also high. These results suggest that SBB07 have good potential for application as probiotic lactic acid bacteria.

바이오제닉 아민 비생성 유산균의 선별을 위해 순창군에서 재배된 베리류 및 엑기스, 식초 등으로부터 42종의 유산균을 분리하였다. 분리주를 대상으로 세포외 효소 활성, 항균 활성, 항산화 활성과 같은 다양한 생리활성 분석을 실시하여 5종의 분리주를 1차 선별하였다. 분리주를 대상으로 바이오제닉 아민 생성 여부를 분석하여 최종적으로 SBB07을 선별하였고, 16S rRNA 염기서열 분석을 통해 Lactobacillus brevis SBB07로 명명하였다. SBB07의 프로바이오틱스 활용 가능성을 확인하기 위해 효소 생산 여부, 내산성, 내담즙성, 내열성, 항생제 내성을 조사하였고, pH 2.0에서의 내산성 및 0.5% oxgall을 함유한 내담즙성에서 각각 86% 이상과 54% 이상의 높은 생존율을 나타내었다. 또한 $60^{\circ}C$에서도 113% 이상의 생존율을 나타내어 내열성도 우수하며 다양한 항생제 내성을 갖는 것을 확인하였다. 프리바이오틱스 이용가능성을 확인한 결과에서는 GOS의 이용 능력이 가장 우수하였으며, FOS와 inulin의 이용 능력도 높게 나타났다. 따라서 SBB07은 세포외 효소 및 항균 활성이 우수하고, 내담즙, 내산성, 내열성 및 항생제 내성을 지니며 프리바이오틱스 기질 이용 능력도 우수한 프로바이오틱스 소재로 활용가능성이 높은 유산균으로 기대된다.

Keywords

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