• 한국발생생물학회:학술대회논문집
• /
• 한국발생생물학회 2001년도 후기 제12차 학술대회 논문집
• /
• pp.34-35
• /
• 2001

RAPD analysis based on numerous markers have been used to investigate patterns of genetic differentiation ann and within two cultured and wild populations represented by the species crucian carp(Carassius carassius). From RAPD analysis using five primers, a total of 442 polymorphic bands were obtained in two populations and 273 were found to be specific to a wild population. According to RAPD-based estimates, average number of polymorphic bands in wild population was approximately 1.5 times as diverse as that in cultured. The average level of bandsharing values was $0.40 \pm 0.05$ in wild population, but was $0.69 \pm 0.08$ in cultured population, With reference to bandsharing values and banding patterns, wild population was considerably more diverse than cultured population. Knowledge of the genetic diversity of crucian carp should help in formulating more effective strategies for managing this aquacultural fish species.

• International Journal of Computer Science & Network Security
• /
• 제21권3호
• /
• pp.1-10
• /
• 2021

In recent decades, the heterogeneous and dynamic behavior of Internet traffic has placed new demands on the adaptive resource allocation of the optical network infrastructure. However, the advent of multifiber elastic optical networks has led to a higher degree of spectrum fragmentation than conventional flexible grid networks due to the dynamic and random establishment and removal of optical connections. In this paper, we propose heuristic routing and dynamic slot allocation algorithms to minimize spectrum fragmentation and reduce the probability of blocking future connection requests by considering the power consumption in elastic multifiber elastic optical networks.

• Investigative Magnetic Resonance Imaging
• /
• 제21권1호
• /
• pp.9-19
• /
• 2017

Background: Normalized cerebral blood volume (nCBV) can be measured using manual or semiautomatic segmentation method. However, the difference in diagnostic performance on brain tumor differentiation between differently measured nCBV has not been evaluated. Purpose: To compare the diagnostic performance of manually obtained nCBV to that of semiautomatically obtained nCBV on glioblastoma (GBM) and primary central nervous system lymphoma (PCNSL) differentiation. Materials and Methods: Histopathologically confirmed forty GBM and eleven PCNSL patients underwent 3T MR imaging with dynamic susceptibility contrast-enhanced perfusion MR imaging before any treatment or biopsy. Based on the contrast-enhanced T1-weighted imaging, the mean nCBV (mCBV) was measured using the manual method (manual mCBV), random regions of interest (ROIs) placement by the observer, or the semiautomatic segmentation method (semiautomatic mCBV). The volume of enhancing portion of the tumor was also measured during semiautomatic segmentation process. T-test, ROC curve analysis, Fisher's exact test and multivariate regression analysis were performed to compare the value and evaluate the diagnostic performance of each parameter. Results: GBM showed a higher enhancing volume (P = 0.0307), a higher manual mCBV (P = 0.018) and a higher semiautomatic mCBV (P = 0.0111) than that of the PCNSL. Semiautomatic mCBV had the highest value (0.815) for the area under the curve (AUC), however, the AUCs of the three parameters were not significantly different from each other. The semiautomatic mCBV was the best independent predictor for the GBM and PCNSL differential diagnosis according to the stepwise multiple regression analysis. Conclusion: We found that the semiautomatic mCBV could be a better predictor than the manual mCBV for the GBM and PCNSL differentiation. We believe that the semiautomatic segmentation method can contribute to the advancement of perfusion based brain tumor evaluation.

• Journal of Microbiology and Biotechnology
• /
• 제20권4호
• /
• pp.683-692
• /
• 2010

Morphologically, nine different slow-growing protoclones were screened from regenerated protoplasts of heterokaryotic Agaricus bisporus. As such, the present study is the first report on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Among 80 primers tested, the seven ISSR and seven RAPD primers selected for the analysis generated a total of 94 ISSR and 52 RAPD fragments, respectively. The ISSR fingerprinting also detected more polymorphic loci (38.29%) than the RAPD fingerprinting (34.61%). A principal coordinate analysis (PCA) was employed to evaluate the resolving power of the markers as regards differentiating protoclones. As a result, the mean polymorphism information content (PIC) for each marker system (i.e., 0.787 for RAPD and 0.916 for ISSR) suggested that ISSR is more effective for determining polymorphisms. The dendrograms constructed using RAPD, ISSR, and an integrated RAPD and ISSR marker system were highly correlated with one another as revealed by a high Mantel correlation (r= 0.98). The pairwise similarity index values also ranged from 0.64 to 0.95 (RAPD), 0.67 to 0.98 (ISSR), and 0.67 to 0.98 (RAPD and ISSR), whereas the mean similarity index values of 0.82, 0.81, and 0.84 were obtained for the RAPD, ISSR, and combined data, respectively. As there was a good correspondence between the RAPD and ISSR similarity matrices, ISSR would appear to be an effective alternative to RAPD in the genetic diversity assessment and accurate differentiation of homo- and heterokaryotic protoclones of A. bisporus.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권2호
• /
• pp.157-162
• /
• 2018

Objective: The aim of this study is to identify single nucleotide polymorphisms (SNPs) and genes related to pig IMF and estimate the heritability of intramuscular fat content (IMF). Methods: Genome-wide association study (GWAS) on 704 inbred Berkshires was performed for IMF. To consider the inbreeding among samples, associations of the SNPs with IMF were tested as random effects in a mixed linear model using the genetic relationship matrix by GEMMA. Significant genes were compared with reported pig IMF quantitative trait loci (QTL) regions and functional classification of the identified genes were also performed. Heritability of IMF was estimated by GCTA tool. Results: Total 365 SNPs were found to be significant from a cutoff of p-value <0.01 and the 365 significant SNPs were annotated across 120 genes. Twenty five genes were on pig IMF QTL regions. Bone morphogenetic protein-binding endothelial cell precursor-derived regulator, forkhead box protein O1, ectodysplasin A receptor, ring finger protein 149, cluster of differentiation, tyrosine-protein phosphatase non-receptor type 1, SRY (sex determining region Y)-box 9 (SOX9), MYC proto-oncogene, and macrophage migration inhibitory factor were related to mitogen-activated protein kinase pathway, which regulates the differentiation to adipocytes. These genes and the genes mapped on QTLs could be the candidate genes affecting IMF. Heritability of IMF was estimated as 0.52, which was relatively high, suggesting that a considerable portion of the total variance of IMF is explained by the SNP information. Conclusion: Our results can contribute to breeding pigs with better IMF and therefore, producing pork with better sensory qualities.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
• /
• 제24권2호
• /
• pp.83-89
• /
• 2013

Objectives : It is known that children with attention-deficit hyperactivity disorder (ADHD) experience significant difficulty in recognizing facial emotion, which involves processing of emotional facial expressions rather than speech, compared to children without ADHD. This objective of this study is to investigate the differences in facial emotion recognition between children with ADHD and normal children used as control. Methods : The children for our study were recruited from the Suwon Project, a cohort comprising a non-random convenience sample of 117 nine-year-old ethnic Koreans. The parents of the study participants completed study questionnaires such as the Korean version of Child Behavior Checklist, ADHD Rating Scale, Kiddie-Schedule for Affective Disorders and Schizophrenia-Present and Lifetime Version. Facial Expression Recognition Test of the Emotion Recognition Test was used for the evaluation of facial emotion recognition and ADHD Rating Scale was used for the assessment of ADHD. Results : ADHD children (N=10) were found to have impaired recognition when it comes to Emotional Differentiation and Contextual Understanding compared with normal controls (N=24). We found no statistically significant difference in the recognition of positive facial emotions (happy and surprise) and negative facial emotions (anger, sadness, disgust and fear) between the children with ADHD and normal children. Conclusion : The results of our study suggested that facial emotion recognition may be closely associated with ADHD, after controlling for covariates, although more research is needed.

• 대한전자공학회논문지SP
• /
• 제47권5호
• /
• pp.136-144
• /
• 2010

동영상 복사 검출을 위해서 사용되는 내용기반 정보는 크게 공간적인 정보와 시간적인 정보로 나눌 수 있다. 이 중에 공간적인 정보는 내용기반 영상 지문(fingerprint)을 의미한다. 이러한 영상 지문이 갖추어야 할 바람직한 특성으로는 추출이 간단해야 하며, 서로 다른 영상에서는 어느 정도 거리를 갖는 독립성(independence)을 가져야 하되, 변형된 영상과 원본 영상 사이에서는 짧은 거리가 나와야 하는 강인성을 가져야 하며 또한 정합 속도가 빨라야 한다. 본 논문에서는 내용기반 동영상 복사검출을 위해 공간 정보를 사용한 새로운 영상 지문을 제안한다. 제안하는 방법에서는 블록 영역의 평균과 1차, 2차 미분 값을 사용하는데 이러한 방법은 특징 추출 시 덧셈과 뺄셈만을 사용하므로 빠른 추출이 가능하고, 강인성과 독립성을 모두 가진다. 또한 사용된 특징 비교를 통해 2진화하고, 거친 지문과 미세 지문의 단계적 처리 방법을 사용하여 빠른 정합이 가능하다. 본 논문에서는 MPEG-7의 VCE7실험 조건을 사용하였으며 다수의 변형된 영상을 이용하여 제안된 방법의 성능을 검증 하였다.

• Archives of Plastic Surgery
• /
• 제40권6호
• /
• pp.676-686
• /
• 2013

Background To overcome the potential drawbacks of a short half-life and dose-related adverse effects of using active transforming growth factor-beta 1 for cartilage engineering, a cell-mediated latent growth factor activation strategy was developed incorporating latent transforming growth factor-${\beta}$1 (LTGF) into an electrospun poly(L-lactide) scaffold. Methods The electrospun scaffold was surface modified with NH3 plasma and biofunctionalised with LTGF to produce both random and orientated biofunctionalised electrospun scaffolds. Scaffold surface chemical analysis and growth factor bioavailability assays were performed. In vitro biocompatibility and human nasal chondrocyte gene expression with these biofunctionalised electrospun scaffold templates were assessed. In vivo chondrogenic activity and chondrocyte gene expression were evaluated in athymic rats. Results Chemical analysis demonstrated that LTGF anchored to the scaffolds was available for enzymatic, chemical and cell activation. The biofunctionalised scaffolds were non-toxic. Gene expression suggested chondrocyte re-differentiation after 14 days in culture. By 6 weeks, the implanted biofunctionalised scaffolds had induced highly passaged chondrocytes to re-express Col2A1 and produce type II collagen. Conclusions We have demonstrated a proof of concept for cell-mediated activation of anchored growth factors using a novel biofunctionalised scaffold in cartilage engineering. This presents a platform for development of protein delivery systems and for tissue engineering.

• The Plant Pathology Journal
• /
• 제37권6호
• /
• pp.619-631
• /
• 2021

Alfalfa mosaic virus (AMV), an economically important pathogen, is present worldwide with a very wide host range. This work reports for the first time the infection of Vinca minor and Wisteria sinensis with AMV using RNA sequencing and reverse transcription polymerase chain reaction confirmation. De novo assembly and annotating of contigs revealed that RNA1, RNA2, and RNA3 genomic fragments consist of 3,690, 2,636, and 2,057 nucleotides (nt) for IR-VM and 3,690, 2,594, and 2,057 nt for IR-WS. RNA1 and RNA3 segments of IR-VM and IR-WS closely resembled those of the Chinese isolate HZ, with 99.23-99.26% and 98.04-98.09% nt identity, respectively. Their RNA2 resembled that of Canadian isolate CaM and American isolate OH-2-2017, with 97.96-98.07% nt identity. The P2 gene revealed more nucleotide diversity compared with other genes. Genes in the AMV genome were under dominant negative selection during evolution, and the P1 and coat protein (CP) proteins were subject to the strongest and weakest purifying selection, respectively. In the population genetic analysis based on the CP gene sequences, all 107 AMV isolates fell into two main clades (A, B) and isolates of clade A were further divided into three groups with significant subpopulation differentiation. The results indicated moderate genetic variation within and no clear geographic or genetic structure between the studied populations, implying moderate gene flow can play an important role in differentiation and distribution of genetic diversity among populations. Several factors have shaped the genetic structure and diversity of AMV: selection, recombination/reassortment, gene flow, and random processes such as founder effects.

• 한국통신학회논문지
• /
• 제29권1B호
• /
• pp.86-100
• /
• 2004

To provide the end-to-end service differentiation for assured services, the random early demotion and promotion (REDP) marker in the edge router at each domain boundary monitors the aggregate flow of the incoming in-profile packets and demotes in-profile packets or promotes the previously demoted in-profile packets at the aggregate flow level according to the negotiated interdomain service level agreement (SLA). The REDP marker achieves UDP fairness in demoting and promoting packets through random and early marking decisions on packets. But, TCP fairness of the REDP marker is not obvious as for UDP sources. In this paper, to improve TCP fairness of the REDP marker, we propose a modified REDP marker where we combine a dropper, meters and a token filling rate configuration component with the REDP marker. To make packet transmission rates of TCP flows more fair, at the aggregate flow level the combined dropper drops incoming excessive in-profile packets randomly with a constant probability when the token level in the leaky bucket stays in demotion region without incoming demoted in-profile packets. Considering the case where the token level cannot stay in demotion region without the prior demotion, we propose a token filling rate configuration method using traffic meters. By using the token filling rate configuration method, the modified REDP marker newly configures a token filling rate which is less than the negotiated rate determined by interdomain SLA and larger than the current input aggregate in-profile traffic rate. Then, with the newly configured token filling rate, the token level in the modified REDP marker can stay in demotion region pertinently fir the operation of the dropper to improve TCP fairness. We experiment with the modified REDP marker using ns2 simulator fur TCP sources at the general case where the token level cannot stay in demotion region without the prior demotion at the negotiated rate set as the bottleneck link bandwidth. The simulation results demonstrate that through the combined dropper with the newly configured token filling rate, the modified REDP marker also increases both aggregate in-profile throughput and link utilization in addition to TCP fairness improvement compared to the REDP marker.