• Journal of Biosystems Engineering
• /
• v.37 no.6
• /
• pp.411-419
• /
• 2012

Purpose: Nondestructive evaluation of seed viability is a highly demanded technique in the seed industry. In this study, hyperspectral imaging system was used for discrimination of viable and non-viable radish seeds. Method: The spectral data with the range from 400 to 1000 nm measured by hyperspectral reflectance imaging system were used. A calibration and a test models were developed by partial least square discrimination analysis (PLS-DA) for classification of viable and non-viable radish seeds. Either each data set of visible (400~750 nm) and NIR (750~1000 nm) spectra and the spectra of the combined spectral ranges were used for developing models. Results: The discrimination accuracy of calibration was 84% for visible range and 76.3% for NIR range. The discrimination accuracy of test was 84.2% for visible range and 75.8% for NIR range. The discrimination accuracies of calibration and test with full range were 92.2% and 92.5%, respectively. The resultant images based on the optimal PLS-DA model showed high performance for the discrimination of the nonviable seeds from the viable seeds with the accuracy of 95%. Conclusions: The results showed that hyperspectral reflectance imaging has good potential for discriminating nonviable radish seeds from massive amounts of viable seeds.

• Korean Journal of Plant Resources
• /
• v.34 no.6
• /
• pp.575-599
• /
• 2021

The edible roots of radish (Raphanus sativus L.) are consumed worldwide. For characterization and evaluation of the agronomic traits and health-promoting chemicals in radish germplasms, new germplasm breeding materials need to be identified. The objectives of this study were to evaluate the phenotypic traits and glucosinolate contents of radish roots from 110 germplasms, by analyzing correlations between 10 quantitative phenotypic traits and the individual and total contents of five glucosinolates. Phenotypic characterization was performed based on descriptors from the UPOV and IBPGR, and glucosinolate contents were analyzed using liquid chromatography-tandem mass spectrometry in multiple reaction monitoring mode (MRM). Regarding the phenotypic traits, a significant correlation between leaf length and root weight was observed. Glucoraphasatin was the main glucosinolate, accounting for an average of 71% of the total glucosinolates in the germplasms; moreover, its content was significantly correlated with that of glucoerucin, its precursor. Principal component analysis indicated that the 110 germplasms could be divided into five groups based on their glucosinolate contents. High levels of free-radical scavenging activity (DPPH) were observed in red radishes. These results shed light on the beneficial traits that could be targeted by breeders, and could also promote diet diversification by demonstrating the health benefits of various germplasms.

• Journal of Applied Biological Chemistry
• /
• v.57 no.2
• /
• pp.153-157
• /
• 2014

Plant-growth-promoting rhizobacteria can affect plant growth by various direct and indirect mechanisms. This study was conducted to determine the ability of some rhizobacterial strains to enhance the seed germination of Lactuca sativa (lettuce) and Raphanus sativus (radish). Seeds were inoculated using a spore suspension ($1{\times}10^7cfumL^{-1}$) and incubated in a growth chamber at $28^{\circ}C$ under dark conditions and 65% RH. Azotobacter chroococcum and LAP mix inoculation increased the plumule length of L. sativa by 1.3, 0.8, and 0.7 cm, respectively, in comparison to the uninoculated control. Pseudomonas putida showed an increase of only 0.6 cm in plumule length when compared to the control. Inoculation of A. chroococcum, P. putida, and LAP mix enhanced the seed germination rate of R. sativus, by 10, 5, and 30%, respectively, in comparison with the uninoculated seeds. The results demonstrated that the inoculation of seeds by select rhizobacterial strains showed remarkable enhancement to the radicle length of lettuce and radish seedlings.

• Korean Journal of Plant Resources
• /
• v.31 no.5
• /
• pp.453-465
• /
• 2018

Radish (Raphanus sativus L.) is a species of the Brassicaceae family and an important root vegetable crop, produced worldwide. A total of 82 radish accessions with various morphological and physiological characteristics analyzed for total sugars content. These accessions includes five subspecies and classified as wild, wild-relative, traditional and improved cultivar. The four sugars, glucose, fructose, sucrose and maltose, showed various contents in 82 accessions. Total sugar content ranged from 5.64 to 46.68 mg/g and showed average 25.33 mg/g. Total sugar content was not statistically significant among the five subspecies, but individual sugar ratio varied. The wild, wild-relatives and traditional cultivars were not significantly among average total sugars content compared with improved cultivars. On the other hand, the wild and traditional cultivars were showed high ratio of individual sugars. These results could be valuable information for the development of new radish cultivars and regulation of sugars biosynthesis in radish.

• Horticultural Science & Technology
• /
• v.32 no.3
• /
• pp.382-389
• /
• 2014

Raphanus sativus L. cv. Taebaek, a efficiently microspore-derived embryo (MDE)-forming cultivar, and 'Chungwoon', a non-MDE-forming cultivar were selected as donor plants for isolated microspore culture. Radish flower bud of 2.0 (small, S), 4.0 (medium, M), and 6.0 (large, L) ${\pm}$ 0.5 mm in length were isolated to determine the temporal relationship between flower bud size and MED yield. Anatomical observations revealed no difference in the structure of the flower buds between the two cultivars. In both cultivars, the stigmas were much longer than the floral leaf in M-sized flower buds. The MDE yields for 'Taebaek' per petri dish were 6.6 and 1.3 for M- and L-sized of flower buds, respectively, but MDE formation was not induced in the S flower buds. On the other hand, 'Chungwoon' failed to form MDEs in all flower buds. The microspore density of 'Taebaek' was 1.3 times more than that of 'Chungwoon' for M sized flower buds. Of the M-sized buds from 'Taebaek' and 'Chungwoon', 92.1 and 81.6%, respectively, were in the late uninucleate microspore stage, which is characterized by the highest frequency of MDE formation. Anatomical observations of MDE formation revealed that the microspores were able to divide to form a primordium from which cell division took place continuously in the 'Teabeak' cultivar. However, the microspores of 'Chungwoon' failed to progress beyond the primodium stage, resulting in lack of MDE formation. By contrast, after the formation of the primordium, various developmental stages of embyos from microspore were observed in the 'Taebaek' cultivar. These results can be used to determine MDE forming potentials of radish cultivars.

• Korean Journal of Environmental Agriculture
• /
• v.34 no.3
• /
• pp.204-209
• /
• 2015

BACKGROUND: Radish (Raphanus sativus L.) is cultivated worldwide and one of important vegetables in Korea where year-round production of radish is possible. Most of radishes in autumn are cultivated with double-row spacing except for Gangwon-do where sing-row spacing is predominant. However, no research has been conducted on double-row spacing in radish cultivation so far. This study was conducted to reveal the optimum double-row spacing in autumn cultivation of radish. METHODS AND RESULTS: Using top two popular autumn radish cultivars 'S' and 'C', seeds were sown in spacing between rows of $55{\times}25$, $45{\times}25$, $35{\times}25$ and $25{\times}25cm$, and that within rows of $35{\times}28$, $35{\times}25$, $35{\times}22$ and $35{\times}19cm$. Plants were harvested 58 days after sowing and leaf weight, length and number, and root weight, length sugar content and pithiness were investigated. In the spacing between rows, no significant difference was observed in leaf weight, length and number in both cultivars; however, 25 cm of spacing between rows significantly reduced the root length and weight in 'S' and 'C' cultivars, respectively. In spacing within rows, 28, 25 and 22 cm did not affect fresh root weight in both cultivars, producing appropriate radish roots of 1,500g on average. However, 19 cm of spacing within rows did not reduced fresh root weight in 'S' cultivar but did significantly in 'C' cultivar (1148.3 g). Sugar contents and pithiness of roots were also affected by spacing but its effect was very small and different between cultivars. CONCLUSION: It is suggested that optimum double-row spacing in autumn radish cultivation is 35 cm and 22 cm of spacing between and within rows, respectively, the spacing that did not reduce the yield and quality of radish roots in two popular autumn radish cultivars.

• Korean Journal of Weed Science
• /
• v.15 no.3
• /
• pp.197-205
• /
• 1995

The objectives of the present study were to find out 1)the differences in growth of radish under foggy and non-foggy condition, 2)the weed competition between radish and major upland weeds including Echinochloa crus-galli, 3)difference in response of radish to herbicide and weed, and finally 4)the fundamental data for the establishment of radish weed control system under locational foggy regions. The research was carried out by tray in greenhouse equipped with Auto Foggy System(SAE KI RIN Co.) and results were summarized as follows. 1. Plant height and shoot fresh weight of radish cultivars(long skirt, delicious altari, green mascot) were not different at the early growth(8 to 38 days after seeding), but increased at 55 days after seeding under foggy condition. Root fresh weight increased only in green mascot cultivar tinder foggy condition. Chlorophyll contents were higher in all the cultivars of radish under foggy condition. 2. Plant height and shoot fresh weight increased at 0 and 20 days of weed competition under foggy condition. Root fresh weight increased at 0, 20 and 35 days of competition under foggy condition. Above 50 days-competition, there was no difference between foggy and non-foggy condition. 3. Napropamide, alachlor and linuron did not show any differences in plant height and shoot fresh weight between foggy and non-foggy condition. Molinate reduced the root fresh weight at 65 days after seeding tinder foggy condition. 4. No difference in weeding efficacy by any herbicides tested was found tinder foggy condition. Weeding efficacy of Aeschynomene indica was reduced under foggy condition.

• Horticultural Science & Technology
• /
• v.32 no.2
• /
• pp.210-216
• /
• 2014

The distribution of S-haplotypes and genetic relationships were evaluated for 47 accessions of 'Danji' radish (Raphanus sativus L. var. hortensis Baker f. gigantissimus Makino) originating from Jeju Island in South Korea. A total of 22 S-haplotype-specific SCAR markers for the S locus glycoprotein (SLG) and S receptor kinase (SRK) loci were tested, and six primer sets amplified locus-specific PCR fragments from at least one 'Danji' radish accession. S5 and S21 alleles atthe SLG locus were the most frequently distributed, and detected from 87.5% and 64.6% of the accessions, respectively. The frequency of the class-II haplotype at the SLG locus was 75%, more frequent than the class-I haplotype. The S23 allele at the SRK locus was detected from 7 accessions. Grouping of the accessions based on S-allele composition revealed three major groups, while 8 accessions showed a unique allelic composition. The genetic diversity of 47 'Danji' radishes and 1 'Gwandong' radish were also evaluated with 38 RAPD primers. A total of 312 bands were scored, and showed that 138 bands (44.2%) were monomorphic among the accessions, whereas 174 (55.8%) bands were polymorphic. Polymorphism rates ranged from 0.2 to 1.0, indicating significant variations in detecting polymorphism across RAPD primers. The genetic similarity coefficients among all pairs of the 48accessions varied from 0.62 to 0.93, and 42% of the comparisons exhibited values higher than 0.85. All the cultivars could be distinguished based on the DNA fingerprints revealed by RAPD. The comparisons between the dendrograms based on S-haplotypes and RAPDs indicate an unrelated and sporadic distribution for several accessions; however, there was a tendency for accessions with the same S-allelic composition to group into the same cluster.

• Food Science and Preservation
• /
• v.14 no.5
• /
• pp.487-491
• /
• 2007

This study was conducted to investigate the effect of treatment with squeous chlorine dioxide and hot water on the germination of radish (Raphanus sativus L.) seeds, and reduction of microbial load on the seeds. Increases in treatment and the concentration of aqueous chlorine dioxide in water resulted in increasing reductions in the counts of total aerobic microbes. Seeds treated with aqueous chlorine dioxide (100 ppm/20min, 200ppm/20min) showed about a 10-fold decrease in microbial loads. Germination of seeds was not adversely affected by any treatment tested, although the germination rate of seeds in the group treated at $55^{\circ}C$ for 20 min was reduced by 10% compared to that of control. Combined treatment with hot water and aqueous chlorine dioxide yielded better out comes in both microbial reduction and seed germination rate than did single treatments. A combined treatment with 100 ppm aqueous chlorine dioxide and hot water($45^{\circ}C$ or $50^{\circ}C$) resulted in about a 100-fold decrease in microbial load whereas germination rate showed only a slight increase to $97.0{\sim}97.7%$. Total aerobic microbial counts in radish seeds were decreased by aqueous chlorine dioxide and hot water treatment in the order. aqueous $CIO_2$+ hot water > aqueous $CIO_2$ > chlorinated water > hot water > control.

• Korean Journal of Breeding Science
• /
• v.41 no.3
• /
• pp.194-204
• /
• 2009

Cytoplasmic male sterility (CMS) and fertility restoration have been utilized as valuable tools for $F_1$-hybrid seed production in many crops despite laborious breeding processes. Molecular markers for the selection of CMS-related genes help reduce the expenses and breeding times. A previously reported genomic region containing the Ppr-B gene, which is responsible for restoration of fertility and corresponds to the Rfo locus, was used to develop gene-based or so-called "functional" markers for allelic selection of the restorer-of-fertility gene (Rfo) in $F_1$-hybrid breeding of radish (Raphanus sativus L.) Polymorphic sequences among Rfo alleles of diverse breeding lines of radish were examined by sequencing the Ppr-B alleles. However, presence of Ppr-B homolog, designated as Ppr-D, interferes on specific PCR amplification of Ppr-B in certain breeding lines. The organization of Ppr-D, resolved by genome walking, revealed extended homology with Ppr-B even in the promoter region. Interestingly, PCR amplification of Ppr-D was repeatedly unsuccessful in certain breeding lines implying the lack of Ppr-D in these radishes. Ppr-B could only be successfully amplified for analysis through designing primers based on the sequences unique to Ppr-B that exclude interference from Ppr-D gene. Four variants of Rfo alleles were identified from 20 breeding lines. A combination of three molecular markers was developed in order to genotype the Rfo locus based on polymorphisms among four different variants. These markers will be useful in facilitating $F_1$-hybrid cultivar development in radish.