Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Effects of Inoculation of Rhizomicrobial Strains on Plant Growth at the Early Germination Stage

  • Yoo, Jae Hong (National Institute of Agricultural Sciences and Technology, Rural Development Administration)
  • Received : 2013.11.21
  • Accepted : 2013.11.27
  • Published : 2014.06.30
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Abstract

Plant-growth-promoting rhizobacteria can affect plant growth by various direct and indirect mechanisms. This study was conducted to determine the ability of some rhizobacterial strains to enhance the seed germination of Lactuca sativa (lettuce) and Raphanus sativus (radish). Seeds were inoculated using a spore suspension ($1{\times}10^7cfumL^{-1}$) and incubated in a growth chamber at $28^{\circ}C$ under dark conditions and 65% RH. Azotobacter chroococcum and LAP mix inoculation increased the plumule length of L. sativa by 1.3, 0.8, and 0.7 cm, respectively, in comparison to the uninoculated control. Pseudomonas putida showed an increase of only 0.6 cm in plumule length when compared to the control. Inoculation of A. chroococcum, P. putida, and LAP mix enhanced the seed germination rate of R. sativus, by 10, 5, and 30%, respectively, in comparison with the uninoculated seeds. The results demonstrated that the inoculation of seeds by select rhizobacterial strains showed remarkable enhancement to the radicle length of lettuce and radish seedlings.

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References

  1. Anderson TA, Guthrie EA, and Walton BT (1993) Bioremediation. Environ Sci Technol 27, 2631-6.
  2. Arshad M and Frankenberger WT Jr (1998) Plant growth regulating substances in the rhizosphere: Microbial production and function. Adv Agron 62, 45-151.
  3. Bacilio M, Rodriguez H, and Moreno M (2004) Mitigation of Salt stress in Wheat seedlings by gfp-tagged Azospirillum lipoferum. Biol Fertil Soils 40, 188-93.
  4. Bais P, Weir TL, Perry LG, Gilroy S, and Vivanco JM (2006) The role of root exudates in rhizosphere interactions with plants and other organisms. Annu Rev Plant Biol 57, 233-66. https://doi.org/10.1146/annurev.arplant.57.032905.105159
  5. Benizri E, Courtade A, Picard C, and Guckert A (1998) Role of maize root exudates in the production of auxins by Pseudomonas fluorescens M.3.1: Short communication. Soil Biol Biochem 30, 1481-4. https://doi.org/10.1016/S0038-0717(98)00006-6
  6. Bharathi R, Vivekananthan R, Harish S, Ramanathan A, and Samiyappan R (2004) Rhizobacteria-based bio-formulations for the management of fruit rot infection in chillies. Crop Prot 23, 835-43. https://doi.org/10.1016/j.cropro.2004.01.007
  7. Bloemberg GV and Lugtenberg BJJ (2001) Molecular basis of plant growth promotion and biocontrol by rhizobacteria. Curr Opin Plant Biol 4(Suppl 4), 343-50. https://doi.org/10.1016/S1369-5266(00)00183-7
  8. Bowen GD and Rovira AD (1999) The rhizsphere and its management to improve plant growth. Adv Agron 66, 1-102. https://doi.org/10.1016/S0065-2113(08)60425-3
  9. Brown ME (1982) Seed and root bacterization. Annu Rev Phytopathol 12, 181-97.
  10. De Salamone IEG, Hynes RK, and Nelson LM (2001) Cytokinin production of plant growth promoting rhizobacteria and selected mutants. Can J Miccrobiol 47, 404-11. https://doi.org/10.1139/w01-029
  11. Glick BR (1995) The enhancement of plant growth by free living bacteria. Can J Microbiol 41 (Suppl 2), 109-14. https://doi.org/10.1139/m95-015
  12. Jetiyanon J and Kloepper JW (2002) Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases. J Biol 24(Suppl 3), 285-91.
  13. Jing YD, He ZL, and Yang XE (2007) Role of soil rhizobacteria in phytoremediation of heavy metal contaminated soils. J Zhejiang Univ Sci B 8, 192-207.
  14. Joo GJ, Kim YM, Kim JT, Rhee IK, Kim JH, and Lee IJ (2005) Gibberellins producing rhizobacteria increase endogenous gibberellins content and promote growth of red peppers. J Microbiol 43, 510-5.
  15. Kang SM, Joo GJ, Hamayun M, Na CI, Shin DH, Kim YK et al. (2009) Gibberellin production and phosphate solubilization by newly isolated strain of Acinetobacter calcoaceticus and its effect on plant growth. Biotechnol Lett 31, 277-81. https://doi.org/10.1007/s10529-008-9867-2
  16. Kloepper JW, Schippers B, and Bakker PA (1992) Proposed elimination of the term endorhizosphere. Phytopathology 82, 726-7.
  17. Lalande R, Bissonnette N, Coutlee D, and Antoun H (1989) Identification of rhizobacteria and determination of their plant-growth promoting potential. Plant Soil 115, 7-11. https://doi.org/10.1007/BF02220688
  18. Lubeck PS, Hansen M, and Sorensen J (2000) Simultaneous detection of the establishment of seed inoculated Pseudomonas fluorescens strain DR54 and native soil bacteria on sugar beet root surfaces using fluorescence antibody and in situ hybridization techniques. FEMS Microbiol Ecol 33(Suppl 1), 11-9. https://doi.org/10.1111/j.1574-6941.2000.tb00721.x
  19. Lucy M, Reed E, and Glick BR (2004) Applications of free living plant growth promoting rhizobacteria. Antonie Van Leeuwenhoek 86, 1-25. https://doi.org/10.1023/B:ANTO.0000024903.10757.6e
  20. Lugtenberg B, Chin-A-Woeng T, and Bloemberg G (2002) Microbe-plant interactions: principles and mechanisms. Antonie Van Leeuwenhoek 81, 373-83. https://doi.org/10.1023/A:1020596903142
  21. Medeiros FHV, Silva G, Mariano RLR, and Barros R (2005) Effect of bacteria on the biology of diamondback moth (Plutella xylostella) on cabbage (Brassica oleraceae var. capitata) cv. Midori. Anais da Academia Pernambucana de Ciencia Agronomica 2, 204-12.
  22. Nihorimbere V, Ongena M, Smargiassi M, and Thonart P (2011) Beneficial effect of the rhizosphere microbial community for plant growth and health. Biotechnol Agron Soc Environ 15, 327-37.
  23. Niranjan-Raj S, Shetty NP, and Shetty HS (2004) Seed-bio-priming with Pseudomonas fluorescens isolates, enhances growth of pearl millet plants and induce resistance against downy mildew. Int J Pest Manage 50, 41-8. https://doi.org/10.1080/09670870310001626365
  24. Okon Y and Kapulnik Y (1986) Development and function of Azospirillum inoculated roots. Plant Soil 90, 3-16. https://doi.org/10.1007/BF02277383
  25. Okon Y and Labandera-Gonzalez C (1994) Agronomic applications of Azospirillum: an evaluation of 20 years of worldwide field inoculation. Soil Biol Biochem 26, 1591-601. https://doi.org/10.1016/0038-0717(94)90311-5
  26. Pattern CL and Glick BR (2002) Role of Pseudomonas putida Indoleacetic acid in development of the host plant root system. Appl Environ Microbiol 68, 3795-801. https://doi.org/10.1128/AEM.68.8.3795-3801.2002
  27. Ping LY and Boland W (2004) Signals from the underground: bacterial volatiles promote growth in Arabidopsis. Trends Plant Sci 9, 263-6. https://doi.org/10.1016/j.tplants.2004.04.008
  28. Raju NS, Niranjana SR, Janardhana GR, Prakash HS, Shetty HS, and Mathur SB (1999) Improvement of seed quality and field emergence of Fusarium moniliforme infected sorghum seeds using biological agents. J Sci Food Agric 79, 206-12. https://doi.org/10.1002/(SICI)1097-0010(199902)79:2<206::AID-JSFA167>3.0.CO;2-Y
  29. SAS (2009) SAS/$STAT^{(R)}$ 9.1 User's Guide. SAS Institute Inc., USA.
  30. Shaukat K, Affrasayab S, and Hasnain S (2006) Growth responses of Helianthus annus to plant growth promoting rhizobacteria used as a biofertilizer. J Agric Res 1, 573-81.
  31. Siddiqui IA and Shaukat SS (2002) Resistance against damping-off fungus Rhizoctonia solani systematically induced by the plant-growth-promoting rhizobacteria Pseudomonas aeruginosa (1E-6S(+)) and P. fluorescens (CHAO). J Phytopathol 150, 500-6. https://doi.org/10.1046/j.1439-0434.2002.00783.x
  32. Tripathi S, Tripathi A, Kori DC, and Tiwari S (1998) Effect of tree leaves aqueous extracts on germination and seedlings growth of soyabean. Allelopathy J 5, 75-82.
  33. Vessey JK (2003) Plant growth promoting rhizobacteria as biofertilizers. Plant Soil 255, 571-86. https://doi.org/10.1023/A:1026037216893
  34. Whiting SN, de Souza MP, and Terry N (2001) Rhizosphere bacteria mobilize Zn for hyperaccumulation by Thlaspi caerulescens. Environ Sci Technol 35, 3144-50. https://doi.org/10.1021/es001938v
  35. Zaidi A, Khan MS, Ahemad M, and Oves M (2009) Plant growth promotion by phosphate solubilizing bacteria. Acta Microbiol Imm H 56, 263-84. https://doi.org/10.1556/AMicr.56.2009.3.6
  36. Zhender G, Kloepper J, Changbin Y, and Wei G (1997) Induction of systemic resistance in Cucumber against cucumber beetles (Coleoptera: Crysomelidae) by Plant-Growth-Promoting-Rhizobacteria. J Econ Entomol 90, 391-6. https://doi.org/10.1093/jee/90.2.391