• Title/Summary/Keyword: quantitative study

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Relative Quantification of Glycans by Metabolic Isotope Labeling with Isotope Glucose in Aspergillus niger

  • Choi, Soo-Hyun;Cho, Ye-Eun;Kim, Do-Hyun;Kim, Jin-il;Yun, Jihee;Jo, Jae-Yoon;Lim, Jae-Min
    • Mass Spectrometry Letters
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    • 제13권4호
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    • pp.139-145
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    • 2022
  • Protein glycosylation is a common post-translational modification by non-template-based biosynthesis. In fungal biotechnology, which has great applications in pharmaceuticals and industries, the importance of research on fungal glycoproteins and glycans is accelerating. In particular, the importance of quantitative analysis of fungal glycans is emerging in research on the production of filamentous fungal proteins by genetic modification. Reliable mass spectrometry-based techniques for quantitative glycomics have evolved into chemical, enzymatic, and metabolic stable isotope labeling methods. In this study, we intend to expand quantitative glycomics by metabolic isotope labeling of glycans in Aspergillus niger, a filamentous fungus model, by the MILPIG method. We demonstrate that incubation of filamentous fungi in a culture medium with carbon-13 labeled glucose (1-13C1) efficiently incorporates carbon-13 into N-linked glycans. In addition, for quantitative validation of this method, light and heavy glycans are mixed 1:1 to show the performance of quantitative analysis of various N-linked glycans simultaneously. We have successfully quantified fungal glycans by MILPIG and expect it to be widely applicable to glycan expression levels under various biological conditions in fungi.

정량적 관리 기반 무기체계 시험업무 프로세스 개선 연구 (A Study on the Improvement of the Test Process for Defense Systems Based on Quantitative Management)

  • 나태흠;이주연;김영민
    • 시스템엔지니어링학술지
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    • 제20권spc1호
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    • pp.1-11
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    • 2024
  • Today, the importance of test and evaluation of defense systems is increasing day by day. In performing efficient defense systems test works, process improvement based on quantitative management is essential. The purpose of this paper is to present the results of process improvement for the defense systems test works of the test organization based on quantitative management activities. As a methodology to confirm process improvement performance, the 'MPM(Managing Performance and Measurement)' practice area of the CMMI model was applied. The quantitative management model for defense systems test works was developed so that it could be practically applied not only to the entire test organization but also to the organization at the department level that actually performs the test work. Finally, the application cases of the quantitative management model for defense system test works and the results of process improvement were described.

컬러 영상 분석을 통한 치석의 정량적 평가 (Quantitative Assessment of Denticulus by Color Image Analysis)

  • 신승원;이선주;이성택;김경섭
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 2008년도 심포지엄 논문집 정보 및 제어부문
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    • pp.129-130
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    • 2008
  • In this study, we implement the color image processing algorithm to analyze the quantitative assessment of denticulus by computing spectral characteristics in HGB color domain. We can observe that we can utilize the normalized HGB color feature to assess the quantitative formation of denticulus.

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폐콘덴서의 절록유 중에 함유된 PCB 분석에 관한 연구 (A Study on PCB Analysis of Insulating Oil in Waste Condenser)

  • 김귀자;박재주
    • Environmental Analysis Health and Toxicology
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    • 제4권1_2호
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    • pp.11-17
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    • 1989
  • A quantitative analytical method of highly concentrated PCB is established in the research. With the quantitative analytical method PCB of insulating oil was examined. The following conclusions are derived from this research. 1. The recovery ratio was 95.7% with the quantitative analytical method, which indicates it can be used for the analysis of PCB. 2. PCB concentration of insulation oil in waste condenser was found to be 21.2% thru 13.85% , which was highly concentrated. 3. PCB insulation oil in waste condenser was same as PCB-42.

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GIS기반 산사태재해의 정량적 피해 산정을 위한 고려사항 분석 (Considerations for Quantitative Risk Assessment of Landslides using GIS)

  • 김정옥;김지영;김효중;김용일
    • 한국방재학회:학술대회논문집
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    • 한국방재학회 2008년도 정기총회 및 학술발표대회
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    • pp.645-648
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    • 2008
  • This study provides considerations for quantitative risk assessment of landslide on GIS technology. It shows how the landslide possibility analysis is linked by GIS modeling to provide loss estimation tools for landslide hazards in support of socio-economic loss reduction efforts. Those risk assessment results can deliver factual damage situation prediction to policy making for the landslide damage mitigation.

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정량적 분석에 의한 오류의 영향 측정에 관한 연구 (A Study for Influence Measurement of Error by Quantitative Analysis)

  • 이은서
    • 정보처리학회논문지:소프트웨어 및 데이터공학
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    • 제1권2호
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    • pp.75-80
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    • 2012
  • 소프트웨어 개발 시, 프로세스 개선에 저해 요인이 되는 소프트웨어, 하드웨어, 인적 오류의 문제가 다수 존재한다. 시스템의 저해 요인을 제거하고 동시에 체계적으로 이를 관리하기 위하여 본 논문에서는 오류의 정량적인 분석방안을 제안한다.

AHP를 이용한 연구실적의 정량화 연구 - A연구소 기초연구사업을 중심으로 (A study on the quantitative evaluation of the performance in R&D with AHP)

  • 이석훈
    • 산업경영시스템학회지
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    • 제19권39호
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    • pp.219-227
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    • 1996
  • For the equitable and objective evaluation of the performances of R & D (Research and Development) Projects, the quantitative evaluation of research products was performed. In this research, the AHP(Analytic Hierarchy Process) technique was used to calculate the relative importance(weight) of research products such as patents, thesis, conferences and man-power with make up individual questions. With calculated relative weight of research products, the model that gives the quantitative grades of research products was studied.

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A Comparative Study on Research Strategies for the Architectural Design Evaluation

  • Han, Seung-Hoon;Moon, Jin-Woo
    • Architectural research
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    • 제12권2호
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    • pp.41-52
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    • 2010
  • The aim of this paper is to evaluate the methodological strategies for the architectural design field of study mainly focused on qualitative and quantitative research designs. Firstly, this paper addresses the characteristics of the six approaches including their methodological aspects in general. Each strategy is assessed by the different approaches to give full insights in it. Secondly, it distinguishes the differences among six research approaches especially derived from qualitative and quantitative research designs. The differences are discussed in terms of the strengths and weaknesses of each strategy. Finally, this paper attempts to discuss about possible applications for introducing approaches to the research topic with which the exemplified research topic, a design evaluation system, deals. To investigate the applicability of the design methods employed, the following topic has been stated; how to develop a design evaluation system and what to be considered for unfolding the thrown topic in terms of strategic approaches in the field of architectural design researches reviewed through the study.

호기성 고정생물막반응기에서 동일 질량부하의 수리학적 및 농도충격부하시 반응의 비교 (Comparison of Aerobic Fixed-film Process Response to Quantitative and Hydraulic Shock for the Same Increases in Mass Loading)

  • 안미경;이규훈;박태주
    • 한국환경과학회지
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    • 제3권3호
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    • pp.285-296
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    • 1994
  • The objective of this study was to examine and compare to transient response to quantitative and hydraulic shocks which produce equal changes in mass rate of organic feed in aerobic fixed-film process. The general experimental approach was to operate the system at several growth rates under steady-state(pre-shock) conditions, then to apply step changes during day 3 in dilution rate(hydraulic shock) , or feed concentration(Quantitative shock) at the same organic mass loading rate. Performance was assessed in both the transient state and the new steady-state (post- shock). Shock load of different type did not produced equivalent disruptions of effluent quality for equal increases in mass loading rate. Based on effluent concentrations, a hydraulic and a Quantitative shock at the same mass loading caused equal increase in total effluent COD, but the increase was primarily a result of suspended solids the hydraulic shock and COD in the quantitative shock. The time which effluent COD came to peak values were about 32~48 hours at the low organic loads and 52 ~ 72 hours at the high organic loads, respectively A quantitative shock produced a much greater increase in effluent COD than did a hydraulic shock at the same mass loading. Mean and peak values of effluent concentration weve increased in 2.8~4.2 times at low organic loading rate, 5.2~6.6 times at the high organic loading rate, respectively. Key words : Aerobic fixed-film reactor, Quantitative shock, hydraulic shock, mass loading rate.

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국가 보호지역 통합 시스템의 양적 확대 목표에 대한 평가 (Evaluating Quantitative Expansion Goals of the National Protected Areas Integrated System)

  • 홍진표
    • 한국환경복원기술학회지
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    • 제21권3호
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    • pp.57-65
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    • 2018
  • The study is conducted to establish the National Protected Areas Integrated System(NPAIS) which includes National Protected Areas(NPAs) and other conservation measures in terms of effective ways for biodiversity conservation. Additionally, it is carried out to evaluate progress toward quantitative expansion goals in Aichi biodiversity Target 11. The NPAIS consists of NPAs and other effective area-based conservation measures(OECMs). There are two different types of OECMs. OECMs type I, including water-source protection Areas(WPA), riparian zones(RZ), fishery-resource protection zones (FPZ), and urban natural park zones(UNPZ), is a potential protected area which is recommended to be incorporated into the NPAs for effective management. OECMs type II means development restriction zones(DRZ), natural recreation forests(NRF), and buffer zones for Korea national arboretum(BKNA). As a result of evaluating the quantitative expansion goals of the NPAIS, terrestrial and inland water protected areas exceed 17% of the quantitative goal in Aichi biodiversity Target 11. The larger the area of individual OECMs and the lower the degree of overlap with NPAs, the higher the contribution of them to the terrestrial and inland water protected areas. DRZ contributes to enlarge more than 3% of quantitative expansion. And RZ and NRF contribute more than 1%. The marine protected areas are expanded by $1,425km^2$ through FPZ, but the expanded area is very small as comparing with the total marine area. It adds only 0.321% to the quantitative expansion. The rest of OECMs also has very poor quantitative expansion contributions in the marine area. Consequently, the NPAIS is effective for quantitative expansion of land areas, but not for marine areas.