• Title/Summary/Keyword: quantitative evaluation method

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Simultaneous detection of fungal, bacterial, and viral pathogens in insects by multiplex PCR and capillary electrophoresis

  • Kwak, Kyu-Won;Nam, Sung-Hee;Choi, Ji-Young;Lee, Seokhyun;Kim, Hong Geun;Kim, Sung-Hyun;Park, Kwan-Ho;Han, Myung-Sae
    • International Journal of Industrial Entomology and Biomaterials
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    • v.30 no.2
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    • pp.64-74
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    • 2015
  • Beetles Protaetia brevitarsis seulensis Kolbe (Coleoptera: Cetoniidae) and Allomyrina dichotoma Linn. (Coleoptera: Scarabaeidae) are widely used in traditional medicine, and the number of insect-rearing farms is increasing in South Korea. The purpose of this study was to establish a multiplex PCR-based assay for rapid simultaneous detection of multiple pathogens causing insect diseases. Six insect parasites such as fungi Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae), bacteria Bacillus thuringiensis Berliner (Bacillales: Bacillaceae), Pseudomonas aeruginosa Migula (Pseudomonadales: Pseudomonadaceae), and Serratia marcescens Bizio (Enterobacteriales: Enterobacteriaceae), and Oryctes rhinoceros nudivirus were chosen based on the severity and incidence rate of insect diseases in South Korea. Pathogen-specific primers were designed and successfully applied for simultaneous detection of multiple infectious agents in farm-bred insects P. b. seulensis and A. dichotoma using multiplex PCR and high resolution capillary electrophoresis. Our results indicate that multiplex PCR is an effective and time-saving method for simultaneous detection of multiple infections in insects, and the QIAxcel capillary electrophoresis system is useful for quantitative evaluation of the individual impact of each infectious agent on the severity of insect disease. The approach designed in this study can be utilized for rapid and accurate diagnostics of infection in insect farms.

Quantitative Analysis of Pulp Fiber Characteristics that Affect Paper Properties(I) (종이의 특성에 영향하는 펄프 섬유특성의 정량적 해석(I))

  • 이강진;박중문
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.30 no.2
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    • pp.47-54
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    • 1998
  • Refining is one of the most important processes of fiber treatment that provides optical and physical properties of final paper products. The evaluation method of refining progress is usually freeness (CSF) or wetness (SR) test because of its rapidity and convenience. However, there are some deficiencies in using freeness or wetness test to evaluate pulp fibers accurately because its results are more influenced by fines contents than extent of fibers treatment. The objective of this study is to show the deficiency of wetness in evaluating the refining process. For this, beating is done by varying the beating load. Handsheets are made after beating until 25 and $32^{\circ}C$ SR, and then paper properties are measured. Refined fibers are analyzed by fiber length, fines contents, curl, kink, WRV, and zero-span tensile strength. The results show that longer beating time is required to reach the same wetness at lower beating load. There are differences in the average fiber length, distribution curve of fiber length, fines contents, curl, kink, WRV of long fiber fraction, drainage time, and zero-span tensile strength of rewetted sample at different beating load. At the low beating load in the same wetness, apparent density, breaking length, burst strength, and tear strength are higher, while opacity and air permeability are lower than those of the high beating load. Using Page s equation, which shows the relationship among tensile strength, intrinsic fiber strength, and interfiber bonding strength, interfiber bonding strength is calculated and analyzed to explain final paper properties. At $25^{\circ}C$ SR, interfiber bonding strength is only slightly higher at 2.5kgf beating load, while the intrinsic fiber strength is substantially higher. At $32^{\circ}C$ SR, intrinsic fiber strength is a little bit higher at 2.5kgf beating load, and interfiber bonding strength is remarkably higher than those of 5.6kgf beating load. These results can be used to explain the different properties of the final paper at selected beating loads.

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Evaluation of Significance & Development of Quantitative Measurement System for Acupoint Pigmentation by Cupping Therapy (부항요법에 의한 배수혈 혈색소 변화의 정량적 측정 시스템 개발 및 유의성 평가)

  • Kim, Soo-Byeong;Lee, Na-Ra;Jung, Byung-Jo;Lee, Yong-Heum
    • Korean Journal of Acupuncture
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    • v.28 no.3
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    • pp.63-71
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    • 2011
  • Objectives : The aim of this study is to develop the system and evaluate the optical analytical technique that reflects acupoint pigmentation and extravasated blood by cupping. Methods : We designed the system able to express XYZ coordinate on local skin color. To evaluate measurement-accuracy, we compared with 24 Color Checker Chart by standardized Commission Internationale de l'Eclairage. After confirming the performance of system, we experimented with cupping which was 80 kPa negative pressure for 1 minute on left/right BL13. The X, Y and Z values were converted to R, G and B, $L^*$, $a^*$ and $b^*$, Erythema Index (E.I.), and Melanin Index (M.I). We compared and analyzed two cases on before/after cupping. Results : The R, G and B values which were converted by X, Y and Z values had high linearity as a high level of R-square (R: 0.969, G: 0.996, and B: 0.992). Moreover, we confirmed that it was possible to quantitatively analyzed the change in skin color by cupping using R, G, B, $L^*$, $a^*$, $b^*$, E.I., and M.I. Conclusions : Therefore, we proposed the new analytical technique for objectifying the oriental medical diagnostic method using cupping and optical sensing technique.

The Preliminary Study for Normative Current Perception Threshold values in the Korean Adults (성인의 Current Perception Threshold 예비조사)

  • Park, Dae-Gyu;Chun, Sung-Hong;Jeon, Jae-Soo;Kim, Yong-Ik;Hwang, Kyung-Ho;Park, Wook
    • The Korean Journal of Pain
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    • v.10 no.1
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    • pp.5-10
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    • 1997
  • Background : The Current Perception Threshold (CPT) provides an objective, quantitative gauge of sensory nerve integrity which is obtainable from any cutaneous site. CPT measurement can confirm and quantify or rule out dysfunction of nerve through comparison with established normative values ($Neuval^{TM}$ CPT database). The aim of this study is to compare collected data from Korean adults with $Neuval^{TM}$ CPT database. Method : Normative data from 5 standard test site in face, hand, toe were obtained from 50 healthy adults. Three frequencies(5, 250, 2000 Hz) were stimulated with $Neuromoter^{(R)}$ CPT device. Results : The results of our data were statistically significantly different than Neuval data except in face, but within normal range. Sensory Threshold increased as the frequency of the stimulus changed from 5 Hz to 250 Hz to 2000 Hz., and from face to hand to toe. Conclusion : CPT testing is a valuable neurologic testing modality that is noninvasive and highly reliable for diagnosis and evaluation of sensory nerves where neuropathy is suspected.

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Dynamic Response based System Reliability Analysis of Structure with Passive Damper - Part 2: Assessment of System Failure Probability (수동형 댐퍼를 장착한 구조물의 동적응답기반 신뢰성 해석 - 제2편: 시스템 파괴확률 산정)

  • Kim, Seung-Min;Ok, Seung-Yong
    • Journal of the Korean Society of Safety
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    • v.31 no.5
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    • pp.95-101
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    • 2016
  • This study proposes a multi-scale dynamic system reliability analysis of control system as a method of quantitative evaluation of its performance in probabilistic terms. In this second paper, we discuss the control effect of the viscous damper on the seismic performance of the structure-level failure. Since the failure of one structural member does not necessarily cause the collapse of the structural system, we need to consider a set of failure scenarios of the structural system and compute the sum of the failure probabilities of the failure scenarios where the statistical dependence between the failure scenarios should be taken into account. Therefore, this computation requires additional system reliability analysis. As a result, the proposed approach takes a hierarchial framework where the failure probability of a structural member is computed using a lower-scale system reliability with the union set of time-sequential member failures and their statistical dependence, and the failure probability of the structural system is again computed using a higher-scale system reliability with the member failure probabilities obtained by the lower-scale system reliability and their statistical dependence. Numerical results demonstrate that the proposed approach can provide an accurate and stable reliability assessment of the control performance of the viscous damper system on the system failure. Also, the parametric study of damper capacity on the seismic performance has been performed to demonstrate the applicability of the proposed approach through the probabilistic assessment of the seismic performance improvement of the damper system.

A Study on Quantitative Method of Certificate for Information Security Education Course in the Private Sector (민간부문 정보보호 교육과정의 정량적 인증방법에 관한 연구)

  • Kim, Joo-hee;Cho, Sung-woo;Yoo, Dong-young
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.26 no.2
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    • pp.551-558
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    • 2016
  • The recent convergence in ICT industry has created new businesses as well as other opportunities. However, it entails new convergence threat accompanied by security risks. Even though there are security professionals who are dealing with the situation, there is not enough human resource in risk management. Moreover, the amount of research that studies quality of education and training security personnel is not sufficient. This paper explores the curriculum of information security education in the private sector and reasons out fifteen standard curriculums in four professional fields categorized by job classification. In addition, it provides a weighted score table based on the evaluation indicator for the effective security education certificates in the private sector.

Capacitive Skin Piloerection Sensors for Human Emotional State Cognition (인간의 감정변화 상태 인지를 위한 정전용량형 피부 입모근 수축 감지센서)

  • Kim, Jaemin;Seo, Dae Geon;Cho, Young-Ho
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.39 no.2
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    • pp.147-152
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    • 2015
  • We designed, fabricated, and tested the capacitive microsensors for skin piloerection monitoring. The performance of the skin piloerection monitoring sensor was characterized using the artificial bump, representing human skin goosebump; thus, resulting in the sensitivity of $-0.00252%/{\mu}m$ and the nonlinearity of 25.9 % for the artificial goosebump deformation in the range of $0{\sim}326{\mu}m$. We also verified two successive human skin piloerection having 3.5 s duration on the subject's dorsal forearms, thus resulting in the capacitance change of -6.2 fF and -9.2 fF compared to the initial condition, corresponding to the piloerection intensity of $145{\mu}m$ and $194{\mu}m$, respectively. It was demonstrated experimentally that the proposed sensor is capable to measure the human skin piloerection objectively and quantitatively, thereby suggesting the quantitative evaluation method of the qualitative human emotional state for cognitive human-machine interfaces applications.

Nondestructive Inspection of Launch Vehicle Structural Components (우주 발사체 구조 요소의 비파피검사)

  • Kong, Cheol-Won;Youn, Jong-Hoon;Park, Jae-Sung;Eun, Se-Won;Jang, Young-Soon;Yi, Yeong-Moo
    • Journal of the Korean Society for Nondestructive Testing
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    • v.29 no.4
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    • pp.331-337
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    • 2009
  • Space launch vehicles require highly reliable, lightweight structures. It is thus important to monitor the structural health of these components with nondestructive inspections. In this paper, we studied an example of a nondestructive inspection that was partially applied to the manufacture and inspection of a launch vehicle. Ultrasonic tests, X-rays, tapping, and acoustic emissions comprised the inspection method. A payload fairing, high pressure tank, fastener part, and bonding part were used as hardware to be inspected. We proposed a quantitative standard for debonding inspection of the payload fairing and acoustic emission data for the proof test of the high pressure tank. We analyzed the fracture mode of the sandwich fastener part according to frequency changes. We also proposed a standard specimen for ultrasonic inspection of bonds of different materials. The present analyses and results provide data for evaluation of the launch operation sequence to ensure launch vehicles afford high reliability.

Quantitative Rehabilitation Extent Monitoring for Unilateral Lower Extremity Disabled Patients using Simulated Gait Pattern Analysis (재활환자 모의보행 패턴분석을 이용한 하지 편측 장애자의 정량적 재활상태 모니터링)

  • Moon, Dong-Jun;Kim, Ju-Young;Noh, Si-Cheol;Choi, Heung-Ho
    • Journal of Biomedical Engineering Research
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    • v.35 no.6
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    • pp.227-233
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    • 2014
  • In this paper, to quantitatively evaluate the degree of rehabilitation for the disabled of unilateral lower extremity, we compared the EMG pattern of normal and simulated abnormal gait. The EMG signal was measured at a rate of 1 kHz on the quadriceps and biceps femoris, the pressure sensor was attached to the sole in order to distinguish the gait cycle. Integrated EMG (IEMG) was obtained by the gait cycle, and classified four patterns that were the normal gait pattern, amplitude decrease pattern, reversed pattern, and irregular pattern. For comparison of the patterns, a curve fitting was performed using the trigonometric functions. The result of curve fitting, the method using a variable A that corresponds to the amplitude of the regression curve was able to distinguish the reverse pattern and remaining pattern. The coefficient of determination ($R^2$) representing coincidence of the pattern of the regression curve and EMG was confirmed the biggest value at the normal gait. Therefore, the degree of normal gait can be confirmed using the coefficient of determination. This results show that it is possible to quantitatively confirm the degree of unilateral lower extremity disabled rehabilitation, and it will be contributed to the study of efficient rehabilitation methods by objective analysis.

Evaluation of SPECT Analysis in Patients with Transient Global Amnesia

  • Choe, Bo-Young;Kim, Euy-Neyng;Chung, Yong-An;Sohn, Hyung-Sun;Kim, Sung-Hoon;Chung, Soo-Kyo;Lee, Hyoung-Koo;Suh, Tae-Suk
    • Journal of the Korean Magnetic Resonance Society
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    • v.6 no.1
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    • pp.45-53
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    • 2002
  • Objectives: This study investigated alterations in regional cerebral blood flow (rCBF) in patients with transient global amnesia (TGA) using statistical parametric mapping 99 (SPM99). Methods: Noninvasive rCBF measurements using 99mTc-ethyl cysteinate dimer (ECD) SPECT were performed on 8 patients with TGA who have ongoing symptoms and 17 age matched controls. The relative rCBF maps in patients with TGA and controls were compared. Results: In patients with TGA, significant decreased rCBF was found along the L superior temporal extending to L parietal region of the brain and L thalamus. There were areas of increased rCBF in the R temporal, R frontal region and R thalamus. Conclusion: We could demonstrate decreased perfusion in left cerebral hemisphere and increased perfusion in right cerebral hemisphere in patients with TGA using SPM99. The imbalanced change of rCBF between bilateral cerebral hemisphere in patients with TGA might suggest that imbalanced neuronal activity between the bilateral hemispheres may have strong relationship to the pathogenesis of the TGA. For quantitative SPECT analysis in TGA patients, we recommend SPM99 rather than the ROI method because of its definitive advantages.

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