• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.124-124
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• 2003

The successful development of embryos cloned by nuclear transfer (NT)have been dependent on a wide range of known factors including cell cycle of donor and recipient ooplast, oocyte quality, NT procedure and oocyte activation. The present study compared the development of cloned porcine embryos following different activation treatments. Cumulus-oocyte complexes (COCs) were aspirated from 26 mm follicles of slaughterhouse ovaries and cultured for 22 h in NCSU #23 medium supplemented with 10% porcine follicular fluid, 0.57 mM cysteine, 0.5 g/mL LH, 0.5 g/mL FSH and 10 ng/mL EGF. The COCs were further cultured for an additional 22 h in the same medium at $39{\cird}C$ in an atmosphere of 5% $CO_2$ in air, without hormonal supplements. Primary cultures of fibroblasts isolated from a female fetus on day 40 of gestation were established in DMEM + 15% FCS. For nuclear donation, cells at the 5th-6th passage were cultured in DMEM +0.5% FCS for 5 days in order to arrest the cells in G0/Gl. After enucleation, oocytes were reconstructed by transfer of donor cells and fusion with three DC pulses (1.4 KV/cm, 30 sec) in 0.28 M mannitol containing 0.01 mM $CaCl_2$ and 0.01 mM $MgCl_2$. Eggs were then divided into three treatment groups, control (without further treatment, Group 1), eggs cultured in 10 g/ml cycloheximide (CHX) for 5 h (Group 2), and eggs cultured in 1.9 mM 6-dimethylaminopurine (6-DMAP) for 5 h (Group 3). The eggs were then cultured in sets of 30 in 60 I drops of NCSU#23 supplemented with 4mg/ml BSA (essentially fatty acid free) until day 7 at $39{\circ}C$ in a humidified atmosphere of 5% $CO_2$. On day 4 the culture were fed by adding 20 I NCSU #23 supplemented with 10% FBS. Development rates into blastocysts were significantly higher (P<0.05) in Group 3 embryos compared to Group 1 controls ($27.6 \mu 2.7% vs. 20.1 \mu 4.1%$, respectively), but rates did not differ in Group 2 compared to control ($23.8 \mu 5.7%$). Total cell number in Group 3 blastocysts was however significantly higher (P<0.05) than in Groups 1 and 2 ($44.6 \mu 2.4 vs. 19.9 \mu 1.9 and 21.9 \mu 2.1$, respectively). These results suggest that 6-DMAP is more efficient than cycloheximide in the activation of electrically fused NT oocytes during in vitro production of cloned porcine embryos.

• Journal of Service Research and Studies
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• v.13 no.3
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• pp.147-159
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• 2023

This study is a study to develop a model for measurement and processing of experience data, which is emerging as a core value in quality management of medical services. In the theoretical background, a literature study was conducted on the importance of experience in medical service, measurement and processing of experience data, and realization of patient-centeredness. Based on these literature and theoretical background research results, operational definitions were performed for the following four research variables, and statistical tests were conducted. Hypothesis 1 is the effect of measuring experience data from the perspective of three factors on persona modeling, Hypothesis 2 is the effect of persona modeling on service blueprint visualization, Hypothesis 3 is the effect of service blueprint visualization on realization of patient-centeredness, and Hypothesis 4 is persona modeling This is the effect that modeling has on the realization of patient-centeredness. After data-based testing of factor analysis, reliability analysis, and correlation analysis, all four hypotheses were adopted as a result of verification using regression analysis. In conclusion, in an era where it is difficult to recognize the value of having only good medical staff and medical equipment in hospitals, it was possible to grasp the meaning that what kind of medical service experience is continuously obtained is more important to patients than the effectiveness of medical staff and medical equipment. In the era of the service economy, the core of hospital service competitiveness is providing attractive experiences, which is the real strength of hospitals, so the measurement and processing of experience data, which is the subject of this study, will have an important meaning in realizing patient-centeredness and realizing smart hospitals.

• Journal of Environmental Impact Assessment
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• v.32 no.4
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• pp.203-213
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• 2023

The AERMOD model was the most used, accounting for 89.0%, based on the analysis of the environmental impact assessment reports published in the Environmental Impact Assessment Information Support System (EIASS) between 2021 and 2022. The mismatch of versions between AERMET and AERMOD was found to be 25.3%. There was the operational time discrepancy of 50.6% from industrial complexes, urban development projects between used in the model and applied in estimating pollutant emissions. The results of applying various versions of the AERMET and AERMOD models to both area sources and point sources in both simple and complex terrain in the Gunsan area showed similar values after AERMOD version 12 (15181). Emissions are assessed as 24-hour operation, and the predicted concentration in both simple and complex terrain when using the variable emission coefficient option that applies an 8-hour daytime operation in the model is lowered by 37.42% ~ 74.27% for area sources and by 32.06% ~ 54.45% for point sources. Therefore, to prevent the error in using the variable emission coefficient, it is required to clearly present the emission calculation process and provide a detailed explanation of the composition of modeling input data in the environmental impact assessment reports. Also, thorough reviews by special institutions are essential.

• Journal of Service Research and Studies
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• v.12 no.4
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• pp.106-124
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• 2022

This study examined whether the social support (emotional support, information support) provided by customers through chatbot service affects the satisfaction of chatbot service felt by customers and whether the satisfaction of chatbot service affects loyalty and intention to continue using chatbot service. In order to confirm the moderating effect of social presence of chatbot service, a total of 300 effective data were obtained by conducting an online survey divided into a group that recognizes social presence highly and a group that recognizes low. As a result of the analysis, the path from emotional support to satisfaction of chatbot service was supported in the group that recognized social presence highly, and the path from emotional support to satisfaction of chatbot service was not supported in the group that recognized social presence low, and the difference was confirmed in the hypothesis path coefficient. This is interpreted as the social presence affecting human emotional response.This study can provide implications for the function of social presence of chatbot service in that it applied information support and emotional support, which are two factors of social support, to chatbot service, and demonstrated the relationship between satisfaction, loyalty, and continuous use according to the degree of social presence of chatbot users.

• Journal of the Korea Institute of Building Construction
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• v.23 no.3
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• pp.305-313
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• 2023

With an increasing interest in the commercialization of research results in the present societal climate, especially in the construction industry, preliminary product analysis plays a critical role when introducing a new product to the market. It significantly influences the product's success or failure. In this context, this study aims to investigate the utility of Importance-Performance Analysis (IPA) as a management strategy tool for preliminary analysis in the commercialization of new architectural technologies. The study specifically assesses a smart ball product engineered for pipeline inspection. The evaluation is carried out based on product quality, convenience, and usability categories. Seventeen factors are recognized as sub-items, and a survey is conducted among relevant experts and consumer groups. From the survey, four key items are chosen: "Keep up the good work," "Concentrate here," "Low priority," and "Possible overkill." Suitable strategic measures are derived for each item. By conducting a correlation analysis between product importance and performance, this study offers a method to establish priority directions for future development. This analysis assists in identifying areas that necessitate improvement or additional focus to increase the product's commercial potential. On the whole, this study contributes to understanding and applying Importance-Performance Analysis as a valuable tool in the preliminary analysis and commercialization of novel technologies in the field of architecture.

• Journal of Bio-Environment Control
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• v.32 no.2
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• pp.115-121
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• 2023

The leopard plant has the characteristic of being used for ornamental purposes when there are yellow spots on the leaves, and is widely used as a bed plant for viewing flowers. To set several indicators to predict the growth of crops with ornamental value, and to quantitatively express the relationship between the indicators are necessary. In this study, we determine a model that estimates the leaf area and the number of flower of Farfugium japonicum Kitam. using leaf length and width, and conducting a regression analysis on some regression models. As an indicator for estimating the leaf area and the number of flower, the leaf length and width of F. japonicum were measured and applied to 8 regression models. As a result of regression analysis of 8 models that estimated leaf area and the number of flower, R² values of the linear models were all higher than 0.84 and 0.80. As a result of validation, using the most reliable model among the models for estimating the leaf area and the number of flowering, R² was 0.90 and 0.82, respectively. Using a model that estimates various indicators that can be used for quality evaluation from easy-to-measure morphological factors, the evaluation of ornamental plants will be facilitated.

• Korean Journal of Environmental Agriculture
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• v.41 no.1
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• pp.41-49
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• 2022

BACKGROUND: The main source of ammonia in soils, South Korea is agricultural emissions (e.g., fertilizer application and livestock manure), with the recent emission inventories reporting them to be approximately 80% of the total emissions. Ammonia as a pollutant is originated largely from agricultural activity and is an important contributor to air quality issues in South Korea. The importance of ammonia in agricultural land is also emerging. In this study, the characteristics of ammonia emission from Chinese cabbage cultivation fields with application rates of urea sere were evaluated. METHODS AND RESULTS: The ammonia emission characteristics were investigated at the different urea application rates (0, 160, 320, and 640 kg ha-1) and the ammonia emission factor in the Chinese cabbage cultivation field was calculated. As application rate of urea application increased, ammonia emissions increased proportionally. In 2020 and 2021, cumulative ammonia emissions with urea 320 kg ha^-1 treatment were 39.3 and 35.2 kg ha^-1, respectively for 2020 and 2021. When urea fertilizer was applied, the ammonia emission factors were 0.1217 and 0.1358 NH₄⁺-N kg N kg^-1 in 2020 and 2021, respectively. CONCLUSION(S): Ammonia emissions increased as application rate of urea increased, and the average ammonia emission factor of the Chinese cabbage cultivation field for two years was 0.129 NH₄⁺-N kg N kg^-1.

• The Journal of Engineering Geology
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• v.33 no.2
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• pp.257-273
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• 2023

Hydrochemical characteristics and microbial communities of spring (Samtong), surface water, and groundwater in Cheorwon, Korea, were analyzed. Field surveys and water quality analyses were undertaken at 10 sampling points for five spring, two surface, and three groundwater samples on 15 December 2022. Hydrochemical analysis revealed that most water samples were Ca-HCO₃ type and that water-rock interactions were the predominant mineral source. Radon concentrations were <1 kBq m^-3 for surface water, 1~10 kBq m^-3 for spring water, and 1~1,000 kq m^-3 for groundwater. Microbial cluster analysis showed that the main phyla were Proteobacteria, Planctomyceta, Verrucomicrobia, Acidobacteria, and Actinomycetota.Non-metric multidimensional scaling (NMDS) analysis indicated that water temperature, pH, and Si content were closely related to microorganism content. NMDS and canonical correspondence analysis results revealed that environmental factors affecting spring water were temperature, and Mg and Si concentrations, particularly for Acidobacteria and Proteobacteria, and Pseudomonas brenneri. Both hydrochemical and microbial community analyses yielded similar results at some spring and groundwater sampling points, likely due to the effects of a basalt aquifer.

• Tuberculosis and Respiratory Diseases
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• v.86 no.2
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• pp.82-93
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• 2023

Tuberculosis (TB) is a significant public health concern. Globally, TB is among the top 10 and the leading cause of death due to a single infectious agent. Providing standard anti-TB therapy for at least 6 months is recommended as one of the crucial strategies to control the TB epidemic. However, the long duration of TB treatment raised the issue of non-adherence. Non-adherence to TB therapy could negatively affect clinical and public health outcomes. Thus, directly observed therapy (DOT) has been introduced as a standard strategy to improve anti-TB medication adherence. Nonetheless, the DOT approach has been criticized due to inconvenience, stigma, reduced economic productivity, and reduced quality of life, which ultimately could complicate adherence issues. Apart from that, its effectiveness in improving anti-TB adherence is debatable. Therefore, digital technology could be an essential tool to enhance the implementation of DOT. Incorporating the health belief model (HBM) into digital technology can further increase its effectiveness in changing behavior and improving medication adherence. This article aimed to review the latest evidence regarding TB medication non-adherence, its associated factors, DOT's efficacy and its alternatives, and the use of digital technology and HBM in improving medication adherence. This paper used the narrative review methodology to analyze related articles to address the study objectives. Conventional DOT has several disadvantages in TB management. Integrating HBM in digital technology development is potentially effective in improving medication adherence. Digital technology provides an opportunity to improve medication adherence to overcome various issues related to DOT implementation.

• Journal of Korean Tunnelling and Underground Space Association
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• v.11 no.4
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• pp.395-401
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• 2009

One of the key design factors for the ventilation and safety system at extra long tunnel is the airflow velocity induced by the natural ventilation force. Despite of the importance, it has not been widely studied due to the complicated influencing variables and the relationship among them is difficult to quantify. At this moment none of the countries in the world defines its specific value on verified ground. It is also the case in Korea. The recent worldwide disasters by tunnel fires and demands for better air quality inside tunnel by users require the optimization of the tunnel ventilation system. This indicates why the natural ventilation force is necessary to be thoroughly studied. This paper aims at predicting the natural ventilation force at a 11 km-long tunnel which is in the stage of detailed design and will be the longest vehicle tunnel in Korea. The concept of barometric barrier which can provide the maximum possible natural ventilation force generated by the topographic effect on the external wind is applied to estimate the effect of wind pressure and the chimney effect caused by the in and outside temperature difference is also analyzed.