• Journal of Integrative Natural Science
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• v.11 no.2
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• pp.93-100
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• 2018

Caspases, a family of cysteinyl aspartate-specific proteases plays a central role in the regulation and the execution of apoptotic cell death. Caspase-3 has been proven to be an effective target for reducing the amount of cellular and tissue damage because the activation of caspases-3 stimulates a signalling pathway that ultimately leads to the death of the cell. In this study, Hologram based Quantitative Structure Activity Relationship (HQSAR) models was generated on a series of Caspase-3 inhibitors named 3, 4-dihydropyrimidoindolones derivatives. The best HQSAR model was obtained using atoms, bonds, and hydrogen atoms (A/B/H) as fragment distinction parameter using hologram length 61 and 3 components with fragment size of minimum 5 and maximum 8. Significant cross-validated correlation coefficient ($q^2=0.684$) and non cross-validated correlation coefficients ($r^2=0.754$) were obtained. The model was then used to evaluate the eight external test compounds and its $r^2_{pred}$ was found to be 0.559. Contribution map show that presence of pyrrolidine sulfonamide ring and its bulkier substituent's makes big contributions for improving the biological activities of the compounds.

• The Korean Journal of Ecology
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• v.6 no.3
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• pp.187-197
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• 1983

This report is a series of the investigation of the secondary vegetation and succession at the forest fire area in Dangji-Dong of Kyungsang-pookdo province. The forest fires occurred on April 8, 1982. This investigation was carried out from June 18, 1982 to May 29, 1983 once in each season. Before to fires, the Pinus densiflora was dominant and the woody plants of understory were mainly composed of the Quercus serrata, Q. dentata, Lespedeza maritima and L. macimowiczii. Compared with the florstic composition between the unburned and burned areas after the fires, the floristic composition of unburned area comprises: 79 families, 194 genera, 223 species, 26 varieties and 6 formae (255 kinds of vascular plants). The index of similary shows 0.77 (S$\Phi$renson:1948) in this area. The analyses of the life-form compositions in the unburned and burned area show 32.9% and 29.3% in dormancy form (H), 42.4% and 37.7% in disseminule form($D_1$), 80.8% and 82.2% in radicoid form ($R_5$), and 57.7% and 61.8% in erect form(e) respectively. The biological type shows H-$D_1$-R5-e, which is common in both areas, and erect form is generally prevailing in these communities.

• Smart Structures and Systems
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• v.19 no.5
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• pp.523-538
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• 2017

A series of field vibration tests are conducted on the Runyang Suspension Bridge during both the construction and operational stages. The purpose of this study is devoted to the analysis of the dynamic characteristics of the suspension tower. After the tower was erected, an array of accelerometers was deployed to study the evolution of its modal parameters during the construction process. Dynamic tests were first performed under the freestanding tower condition and then under the tower-cable condition after the superstructure was installed. Based on the identified modal parameters, the effect of the pile-soil-structure interaction on dynamic characteristics of the suspension tower is investigated. Moreover, the stiffness of the pile foundation is successfully identified using a probabilistic finite model updating method. Furthermore, challenges of identifying the dynamic properties of the tower from the coupled responses of the tower-cable system are discussed in detail. It's found that compared with the identified results from the freestanding tower, the longitudinal and torsional natural frequencies of the tower in the tower-cable system have changed significantly, while the lateral mode frequencies change slightly. The identified modal results from measurements by the structural health monitoring system further confirmed that the vibrations of the bridge subsystems (i.e., the tower, the suspended deck and the main cable) are strongly coupled with one another.

• Wind and Structures
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• v.31 no.4
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• pp.363-371
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• 2020

Proper understanding of offshore wind speed variability is of essential importance in practice, which provides useful information to a wide range of coastal and marine activities. In this paper, long-term wind speed data recorded at various offshore stations are analyzed in the framework of fractal dimension analysis. Fractal analysis is a well-established data analysis tool, which is particularly suitable to determine the complexity in time series from a quantitative point of view. The fractal dimension is estimated using the conventional box-counting method. The results suggest that the wind speed data are generally fractals, which are likely to exhibit a persistent nature. The mean fractal dimension varies from 1.31 at an offshore weather station to 1.43 at an urban station, which is mainly associated with surface roughness condition. Monthly variability of fractal dimension at offshore stations is well-defined, which often possess larger values during hotter months and lower values during winter. This is partly attributed to the effect of thermal instability. In addition, with an increase in measurement interval, the mean and minimum fractal dimension decrease, whereas the maximum and coefficient of variation increase in parallel.

• BMB Reports
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• v.44 no.1
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• pp.28-33
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• 2011

MicroRNAs are potential key regulators in mesenchymal stem cells chondrogenic differentiation. However, there were few reports about the accurate effects of miRNAs on chondrogenic differentiation. To investigate the mechanisms of miRNAs-mediated regulation during the process, we performed miRNAs microarray in MSCs at four different stages of TGF-${\beta}3$-induced chondrogenic differentiation. We observed that eight miRNAs were significantly up-regulated and five miRNAs were downregulated. Interestingly, we found two miRNAs clusters, miR-143/145 and miR-132/212, kept on down-regulation in the process. Using bioinformatics approaches, we analyzed the target genes of these differentially expressed miRNAs and found a series of them correlated with the process of chondrogenesis. Furthermore, the qPCR results showed that the up-regulated (or down-regulated) expression of miRNAs were inversely associated with the expression of predicted target genes. Our results first revealed the expression profiles of miRNAs in chondrogenic differentiation of MSCs and provided a new insight on complicated regulation mechanisms of chondrogenesis.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2006.05a
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• pp.1223-1228
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• 2006

This paper presents an experimental investigation of a recently developed Kronecker Product (KP) method to determine the type, location, and intensity of structural damage from an identified state-space model of the system. Although this inverse problem appears to be highly nonlinear, the system mass, stiffness, and damping matrices are identified through a series of transformations, and with the aid of the Kronecker product, only linear operations are involved in the process. Since a state-space model can be identified directly from input-output data, an initial finite element model and/or model updating are not required. The test structure is a two-degree-of-freedom torsional system in which mass and stiffness are arbitrarily adjustable to simulate various conditions of structural damage. This simple apparatus demonstrates the capability of the damage detection method by not only identifying the location and the extent of the damage, but also differentiating the nature of the damage. The potential applicability of the KP method for structural damage identification is confirmed by laboratory test.

• Proceedings of the KIEE Conference
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• 2001.04a
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• pp.33-36
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• 2001

Torque ripple control of brush less DC motor has been the main issue of the servo drive systems in which the speed fluctuation, vibration and acoustic noise should be minimized. Most methods for suppressing the torque ripples require Fourier series analysis and either the iterative or least mean square minimization. In this paper, the novel approach to achieve the ripple-tree torque control with maximum efficiency based on the d-q-0 reference frame is presented. The proposed method optimize the reference phase current waveforms including even the case of 3 phase unbalanced condition, and the motor winding currents are controlled to follow up the optimized current waveforms by delta modulation technique. As a result, the proposed approach provides a simple and clear way to obtain the optimal motor excitation currents. The validity and practical applications of the proposed control scheme are verified through the simulations and experimental results.

• Korean Journal of Poultry Science
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• v.38 no.2
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• pp.145-154
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• 2011

By a series of positional cloning, we successfully narrowed down the AM candidate region to approximately 1.2 Mbp on GGA2q including 7 functional genes. Subsequently, we identified WWP1 gene as the most likely AM candidate by sequence comparison. The amino acid sequence around the candidate mutation was highly conserved among tetrapods, suggesting that WWP1 is the causative gene of chicken muscular dystrophy. Transfection of mutated WWP1 gene into $C_2C_{12}$ myoblasts disrupted muscle differentiation process. The abnormal muscle differentiation is a characteristic of chicken muscular dystrophy, so we could demonstrate a part of phenotype of the disease. Furthermore, western blotting revealed that accumulation of caveolin-3 protein is limited in damaged muscle of muscular dystrophic chicken, suggesting caveolin-3 may be associated with the pathological change of the disease. We could conclude that WWP1 gene is the responsible one for chicken muscular dystrophy from these results, but the mechanism leading the onset should be clarified in the future. The information will contribute to the study of chicken muscular dystrophy and the corresponding human dystrophies.

• Journal of Applied Biological Chemistry
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• v.43 no.3
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• pp.141-146
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• 2000

Glycosidase activities were tested from the grape berries, Vitis labruscana B. Takasumi. Among various glycosidases, $\alpha$-D-galactosidase was found to be the most active in the flesh and other glycosidases were considerably active in the order of the following: $\alpha$-D-mannosidase>$\alpha$-D-glucosidase>$\beta$-D-glucosidase>$\beta$-D-galactosidase. In the seeds, $\alpha$-D-glucosidase activity was the highest and other glycosidases such as $\alpha$-D-galactosidase, $\beta$-D-glucosidase, and $\beta$-D-galactosidase were still significantly active. The $\alpha$-D-galactosidase in the grape flesh was purified over 83-folds through salting-out with $(NH_4)_2SO_4$ and a series of chromatographies employing Sephadex G-50, Octyl-Sepharose, Q-Sepha- rose, and Biogel P-100. The enzyme was a monomer of 45 kDs as determined through SDS-PAGE and Sephacryl S-200 chromatography. The purified enzyme showed a preference of $\alpha$-D-galactose to $\beta$-D-galactose as a substrate about 5.4 times. Sulfhydryl specific reagents such as N-ethylmaleimide and iodoacetamide significantly inhibited the enzyme activity to the extents of 48 and 52% of its initial activity, respectively. The optimumpH range of $\alpha$-D-galactosidase was around 6.5-7.0. The enzyme activity increased by 46% in the presence of 1mM $Fe^{2+}$.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.3
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• pp.237-243
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• 2013

B13 is a ceramide analogue and apoptosis inducer with potent cytotoxic activity. A series of arylpropyl sulfonamide analogues of B13 were evaluated for their cytotoxicity using MTT assays in prostate cancer PC-3 and leukemia HL-60 cell lines. Some compounds (4, 9, 13, 14, 15, and 20) showed stronger activities than B13 in both tumor cell lines, and compound (15) gave the most potent activity with $IC_{50}$ values of 29.2 and 20.7 ${\mu}M$, for PC-3and HL-60 cells, respectively. Three-dimensional quantitative structure-activity relationship (3D-QSAR) analysis was performed to build highly reliable and predictive CoMSIA models with cross-validated $q^2$ values of 0.816 and 0.702, respectively. Our results suggest that long alkyl chains and a 1R, 2R configuration of the propyl group are important for the cytotoxic activities of arylpropyl sulfonamides. Moreover, the introduction of small hydrophobic groups in the phenyl ring and sulfonamide group could increase biological activity.