• Journal of Animal Science and Technology
• /
• 제47권6호
• /
• pp.1001-1008
• /
• 2005

본 연구는 젖소에서 곡류의 가공형태 및 RUP수준이 효과를 구명하기 위하여 분만 전 3주부터 분만 후 3주까지 전환기 Holstein종 경산우 20두(4처리 5반복)에게 TMR 사료를 GCR30 (파쇄옥수수+RUP 30%), GCR40 (파쇄옥수수+RUP40%), FCR30 (후레이크옥수수+RUP 30%), FCR40 (후레이크옥수수+RUP 40%)로 하여, 건유기와 비유기로 구분하여 배합하여 급여하였다. 본 연구로부터 얻어진 결과를 요약하면 다음과 같다. 분만전 사료의 건물 섭취량은 처리별로 평균 14～15kg이었으며, 건유기간 중 사료섭취량에는 유의한 차이가 없었으나, 분만 후 건물섭취량에서는 RUP 40%에서 섭취량이 RUP30%보다 높게 나타났다 (P＜0.05). 유량은 RUP 40 % 사료를 급여한 구 (FCR40과 GCR40)에서 유의하게 높은 결과를 보여주었다. 유단백질과 유지방을 분석한 결과, 유지방에 있어서는 옥수수 가공 형태인 후레이크와 파쇄처리에 따른 유의있는 결과를 보여주지는 않았다. 혈액 내 대사물질은 분만 전에 비하여 분만 당일의 수치가 모든 처리에서 상승하는 것을 볼 수 있었으며, 분만전ㆍ후의 NEFA의 농도를 보면 모든 처리구에서 분만을 기점으로 상승하였으며, 특히 분만 후에 유량이 높았던 FCR40에서 NEFA 수준이 전반적으로 높은 경향을 보였다 (p<0.05). Glucose와 cortisol 수준은 포도당 농도에서 FCR40구의 건유기가 다른 구보다 유의하게 높았으나 타 처리구간에서는 유의차가 인정되지 않았다. Insulin 농도에 있어서도 분만 전에 처리 간에 유의한 차이가 없으나, 분만 후에는 타처리와 비교하여 FCR40 처리에서 유의하게 높은 농도를 보여 주었다 (p<0.05). 이 시험에서는 옥수수의 가공형태보다는 동량의 단백질에 RUP 함량을 30 %에서 40 %로 증가시킴으로서 에너지, 단백질 이용 효율을 높여 젖소의 생산성을 높일 수 있을 것으로 생각된다.

• Journal of Crop Science and Biotechnology
• /
• 제10권3호
• /
• pp.133-140
• /
• 2007

Protein characteristics of Korean wheat were evaluated to determine the effects of protein content and quality on processing and textural properties of white salted noodles compared to US wheat flours with various wheat classes and commercial flours for making noodles. Protein quality parameters, which were independent of protein content and included SDS sedimentation volume with constant protein weight, mixograph mixing time and proportion of 50% 1-propanol insoluble protein, of Korean wheat flours with 2.2+12 subunits in high molecular weight glutenin subunit compositions were comparable to those of commercial flours for making noodles. Parameters related to noodle making, including optimum water absorption, thickness and color of noodle dough sheet, correlated with protein content and related parameters, including SDS sedimentation volume with constant flour weight, mixograph water absorption and gluten yield. No significant relationship was found in protein parameters independent of protein content. Hardness of cooked noodles from Korean wheats was lower than that of US wheat flours compared to similar protein content of commercial noodle flours. Adhesiveness, springiness and cohesiveness of cooked noodles from Korean wheats were similar to US wheat flours. Hardness of cooked noodles correlated with protein content and related parameters.

• 한국축산식품학회지
• /
• 제35권1호
• /
• pp.50-57
• /
• 2015

Fish sarcoplasmic protein (SP) is currently dumped as waste from surimi industry and its recovery by practical method for being the non-meat ingredient in meat industry would be a strategy to utilize effectively the fish resource. This study was aimed to apply pH treatment for fish SP recovery and evaluated its effect on pork myofibrillar protein (MP) gel. The pH values of fish SP were changed to 3 and 12, and neutralized to pH 7 before lyophilizing the precipitated protein after centrifugation. Acid-treated fish SP (AFSP) showed about 4-fold higher recovery yield than that of alkaline-treated SP and water absorption capacity was also about 1.2-fold greater. Because of the high recovery yield and water absorption capacity, AFSP was selected to incorporate into MP with/without microbial transglutaminase (MTG). The effects of AFSP and MTG on the physicochemical and rheological characteristics of MP and MP gel were evaluated. MTG induced an increase shear stress of the MP mixture and increase the breaking force of MP gels. MP gel lightness was decreased by adding AFSP. MP gel with MTG showed higher cooking loss than that without MTG. A reduction of cooking loss was observed when the AFSP was added along with MTG, where the insoluble particles were found. Therefore, AFSP could be contributed as a water holding agent in meat protein gel.

• Asian-Australasian Journal of Animal Sciences
• /
• 제31권1호
• /
• pp.47-53
• /
• 2018

Objective: The objective of this experiment was to investigate the effects of heat stress on milk protein and blood amino acid profile in dairy cows. Methods: Twelve dairy cows with the similar parity, days in milk and milk yield were randomly divided into two groups with six cows raised in summer and others in autumn, respectively. Constant managerial conditions and diets were maintained during the experiment. Measurements and samples for heat stress and no heat stress were obtained according to the physical alterations of the temperature-humidity index. Results: Results showed that heat stress significantly reduced the milk protein content (p<0.05). Heat stress tended to decrease milk yield (p = 0.09). Furthermore, heat stress decreased dry matter intake, the concentration of blood glucose and insulin, and glutathione peroxidase activity, while increased levels of non-esterified fatty acid and malondialdehyde (p<0.05). Additionally, the concentrations of blood Thr involved in immune response were increased under heat stress (p<0.05). The concentration of blood Ala, Glu, Asp, and Gly, associated with gluconeogenesis, were also increased under heat stress (p<0.05). However, the concentration of blood Lys that promotes milk protein synthesis was decreased under heat stress (p<0.05). Conclusion: In conclusion, this study revealed that more amino acids were required for maintenance but not for milk protein synthesis under heat stress, and the decreased availability of amino acids for milk protein synthesis may be attributed to competition of immune response and gluconeogenesis.

• 미생물학회지
• /
• 제16권4호
• /
• pp.170-179
• /
• 1978

By the successive enrichment culture, more than 250 methanol-utilizing bacteria were isolated from various samples such as soil, waste water and sewage. Two strains of which were selected and tentatively identified as Acinetobacter sp. and Pseudomonas sp. experiments were carried out to determine the growth conditions for the higher biomass yield and to demonstrate the difference to protein composition dependent upon carbon sources of these two species. the results were as follows ; 1. the optimum pH was determined as 8 in the both species. The optimum temperature in Acinetobacter sp. was $25^{\circ}C{\sim}30^{\circ}C$ and pseudomonas sp. was $30^{\circ}C-35^{\circ}C$. The optimum initial concentration of mthanol was determined as 1-2% in Acinetobacter sp. and 2-3% in pseudomonas sp. 2. The optimum concnetrations of nitrogen source, micro-elements, and vitamins such as biotin and thiamine-HCl in Acnetobactar sp. were 1g $(NH_4)_3SO4,\;1{\sim}3mg\;Mn^{++},\;4mg\;Fe^{++},\;10{\mu}g\;biotin,\;and\;100{\mu}g$ thiamine-HCl per liter medium. In the Pseudomonas sp., 2g $(NH_4)_3SO4,\;1mg\;Mn^{++},\;trace\;amounts\;of\;Fe^{++},\;5{\mu}g\;biotin,\;and\;100{\mu}g$ thiamine HCl per liter were effective. Maximum biomass yield was 2.5g/l in Acinetobacter sp. and 4.8g/l in Pseudomonas sp. 3. Protein composition of the two strains exhibited that alkai-labile protein was higher than alkali-stable protein. In Pseudomonas sp., the contents of acid soluble fraction and alkali-stable protein of the cells grown in the methanol medium were higher than in sucrose medium. On the other hand, in Acinetobacter sp., alkalilabile protein of the cells grown in sucrose medium was higher than in methanol medium.

• 한국미생물·생명공학회지
• /
• 제49권2호
• /
• pp.148-156
• /
• 2021

Single-chain antibodies against epidermal growth factor receptor variant III (EGFRvIII) are potentially promising agents for developing antibody-based cancer treatment strategies. We described in our previous study the successful expression of an anti-EGFRvIII scFv antibody in Escherichia coli. However, we could also observe the formation of insoluble aggregates in the periplasmic space, limiting the production yield of the active product. In the present study, we investigated the mechanisms by which growth conditions could affect the expression of the soluble anti-EGFRvIII scFv antibody in small-scale E. coli NiCo21(DE3) cultures, attempting to maximize production. The secreted scFv molecules were purified using Ni-NTA magnetic beads and protein characterization was performed using SDS-PAGE and western blot analyses. We used the ImageJ software for protein quantification and determined the antigen-binding activity of the scFv antibody against the EGFRvIII protein. Our results showed that the highest percentage of soluble scFv expression could be achieved under culture conditions that combined low IPTG concentration (0.1 mM), low growth temperature (18℃), and large culture dish surface area. We found moderate-yield soluble scFv production in the culture medium after lactose-mediated induction, which was also beneficial for downstream protein processing. These findings were confirmed by conducting western blot analysis, indicating that the soluble, approximately 30-kDa scFv molecule was localized in the periplasm and the extracellular space. Moreover, the antigen-binding assay confirmed the scFv affinity against the EGFRvIII antigen. In conclusion, our study reveals that low-speed protein expression is preferable to obtain more soluble anti-EGFRvIII scFv protein in an E. coli expression system.

• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2022년도 추계학술대회
• /
• pp.325-325
• /
• 2022

In order to developing high-quality rice varieties and processing varieties, systematic and stable evaluation of physicochemical properties is required for breeding lines. In this study, we compared the quality characteristics evaluation results of rice breeding lines for cooked rice, special rice, and whole crop silage rice adapted to central plain region (Suwon) in order to use as basic data for future rice variety development. Brown rice length/width ratio, head rice ratio, protein content, amylose content, alkali digestion value(ADV) and Toyo value were analyzed to evaluate the quality characteristics of yield trial lines cultivated in Suwon for four years (2017-2020). Brown rice length/width ratio, head rice ratio, protein content, ADV and Toyo values showed significant differences by year, but there was no significant difference in amylose content (p<0.05), which showed little environmental variation. The head rice ratio and Toyo value showed an increasing trend, while the protein content showed a decreasing trend. However, the protein content was the highest in 2020, which is thought to be owing to little sunlight hours due to heavy rainfall in 2020. The protein content of whole crop silage rice was 8.1%, which was significantly higher than that of other lines (p<0.05). Toyo value of medium-maturing and early-maturing lines were 67.6 and 73.7%, respectively, and the Toyo value of medium-maturing lines was higher than that of the early-maturing lines (p<0.05). In correlation analysis among the quality characteristics of the rice lines for cooked rice, significant positive correlations were detected between Toyo value and head rice ratio, amylose content, ADV, and a negative correlation was observed between Toyo value and protein content (p<0.05).

• Journal of Microbiology and Biotechnology
• /
• 제6권4호
• /
• pp.225-231
• /
• 1996

The production of recombinant proteins in Escherichia coli often leads to the formation of an intracellular inclusion body. Key process steps that can determine the economics of large-scale protein production from inclusion bodies are fermentation, inclusion body recovery, and protein refolding. Compared with protein refolding and fermentation, inclusion body recovery has received scant research attention. Nevertheless, it can control the final product yield and hence process cost for some products. Optimal separation of inclusion bodies and cell debris can also aid subsequent operations by removing contaminant particulates that foul chromatographic resins and contain antigenic pyrogens. In this review, the properties of inclusion bodies and cellular debris are therefore examined. Attempts to optimise the centrifugal separation of inclusion bodies and debris are also discussed.

• Korean Chemical Engineering Research
• /
• 제44권1호
• /
• pp.40-45
• /
• 2006

원하는 유전자를 배큘로바이러스의 감염에 의하여 초파리 Drosophila melanogaster S2 세포에 도입하는 배큘로바이러스/S2 세포 발현 시스템은 강력하고 안전한 배큘로바이러스의 장점과 세포가 파괴되지 않는 S2 세포의 장점을 접목한 시스템이다. 본 연구에서는 외래 목적 단백질로 발현 모니터링이 손쉬운 녹색형광단백질(green fluorescent protein)을 발현시키는 재조합 배큘로바이러스를 이용하여 S2 세포에의 감염조건들에 대한 영향 연구를 수행하였다. 재조합 배큘로바이러스의 S2 세포에의 감염조건으로는 multiplicity of infection(MOI), 초기 세포 수, 배큘로바이러스 용액이 세포를 적시는 최소 부피, 배큘로바이러스를 첨가한 후 제거하기까지의 배양시간 그리고 배큘로바이러스 제거 후 S2 세포를 혈청이 존재하는 배지에서 키우는 배양시간을 선정하였다. MOI는 일반적으로 크게 하는 것이 높은 발현 수율을 위하여 좋은 결과를 보였으나 세포배양이 길어지는 경우 세포독성의 문제가 심각해 질 수 있고 실제적인 배양에서는 높은 MOI를 유지하는 것이 불가능하므로 사용하는 100 mm 배양접시에서 배큘로바이러스와 S2 세포가 접촉하는 동안의 변수들인 MOI를 적정의 값인 30으로, 배큘로바이러스 배양 시간을 1.5 시간으로 고정함으로써 배큘로바이러스가 숙주 세포인 S2에 대해 미치는 세포독성을 낮출 수 있었다. 또한, 배큘로바이러스 최소 부피는 배양접시에서 사용부피의 2.4％에서 가장 좋은 결과를 보였으며 배큘로바이러스 용액을 10배 농축시킴으로써 이 부피를 맞추기 위해 첨가시키는 새로운 배지의 비율을 높임으로써 결과적으로 배큘로바이러스에 의한 세포독성을 줄일 수 있었다. 이를 통하여 세포 파쇄 및 배양접시 표면에서 탈착되는 것을 막고 높은 생장 상태를 유지해서 감염 효율도 높일 수 있었다. 배큘로바이러스의 감염이 끝난 후의 관여하는 변수들인 감염 후 배양시간은 24시간에서 최대의 녹색형광단백질의 발현을 나타내었다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권4호
• /
• pp.502-511
• /
• 2005

The effects of different levels of crude protein (CP) and cottonseed meal replacing for soybean meal in cassava chips and rice straw-based diets for mid-lactating cows (100-150 day in milk [DIM]), were studied using 32 multiparous Holstein Fresian crossbred dairy cows. Diets containing 10.5, 12.5, 13.7, 14.4% CP of the rations and 0, 12.1, 14.9, 17.8% cottonseed meal were fed to cows for 60 days. Thirty two cows were randomly divided into four dietary treatments using a Randomized complete block design. Four dietary treatments were offered in the form of total mixed ration (TMR) with concentrate to roughage (chopped rice straw) at 60:40 and offered ad libitum. Dry matter (DM) and neutral detergent fiber (NDF) intakes tended to linearly increase with increasing dietary CP levels. Intakes and digestibility of crude protein increased linearly with increasing dietary CP level (p<0.01). Crude protein digestibility of the 10.5% CP diet was lower (p<0.05) than that in diets with higher levels of CP, while there were no significant differences among the other three levels of CP (12.5, 13.7 and 14.4%). Daily milk yield tended to increase with increased CP from 10.5 to 14.4%. Income over feed in terms of US$/kg of milk increased with increased CP from 10.5 to 13.7% and decreased when the CP level was higher than 13.7% (quadratic effect p<0.09). Milk composition was not significantly affected by increasing level of CP, however there were relatively high contents of protein and fat among treatments. The proportion of milk-urea N (MUN), ammonia-N ($NH_3$-N) and bloodurea N (BUN) were closely correlated and increased linearly with increasing CP levels (p<0.01). Balanced diet was found in diet containing 12.5 and 13.7% CP of the rations when BUN and MUN were used as indicators of the protein to energy ratio in the diet. Conclusions can be made that increasing dietary CP levels from 10.5 to 13.7% using cottonseed meal as the main source to completely replace soybean meal was beneficial to cows consuming rice straw and cassava chips based-diets. Increasing the CP level above 13.7% of total ration did not additionally improve milk yield and composition or net income.