• 한국식물병리학회지
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• 제14권3호
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• pp.229-239
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• 1998

Antibodies were raised against fusion proteins of the N-terminus and a region containing the GDNQ (Gly-Asp-Asn-Gln) polymerase motif of the L (polymerase) protein of sonchus yellow net virus (SYNV). Immunoblot analyses using these antibodies revealed the presence of the L protein in purified SYNV preparations and in nuclear extracts from infected tobacco. The serological analyses and detection in a polyacrylamide gels suggested that the L protein is present in at least a 20 fold lower abundance than the G, N, M1 and M2 proteins, and has size corresponding to a molecular weight of over 200 kDa as predicted from nucleotide sequence data. Electron microscopy with gold-labelled antibodies was used to localize the N, M2, and G proteins of SYNV in thin sections of infected tissue. When sections of SYNV-infected tissue were treated with antisera against total SYNV proteins and N protein, gold label could be detected in both the viroplasms and in virus particles. With the anti-M2 protein antiserum, the gold label was strongly localized in the viroplasms but only limited labelling of the virus particle sonly. Limited labelling of the L protein was observed in the viroplasms and the virus particles, presumably because of the low abundance of L protein in the tissues.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2341-2344
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• 2014

Background: p53 protein expression has been detected immunohistochemically in papillary thyroid carcinoma(PTC). We investigated the relations between its expression and clinicopathologic features and its significance as a diagnostic marker. Materials and Methods: We compared and evaluated 93 patients in whom thyroidectomy with lymph node dissection had been performed to treat PTC for clinicopathologic significance and 102 patients with 23 papillary thyroid overt carcinomas (POC), 57 papillary thyroid microcarcinomas(PMC), 5 follicular adenomas (FA), 5 Hashimoto's thyroiditis (HT) and 12 nodular hyperplasias (NH) for significance as a diagnostic marker. Expression of p53 protein was evaluated immunohistochemically in sections of paraffinembedded tissue. Results: Statistical analysis showed significantly different expression of p53 in PTC versus other benign thyroid lesions (BTL).The diagnostic sensitivity and specificity were 85.0% and 72.7%, respectively. Overexpression of p53 protein was observed in 44 of the 93 PTC cases (47.3%), but no significant correlation between p53 protein overexpression and clinicopathologic features (age, size, multiplicity, lymph node metastasis, extrathyroidal extension and vascular invasion) was noted. Conclusions: p53 is valuable to distinguish PTC from other BTL, but there is no correlation between p53 protein overexpression and clinicopathologic features.

• 대한수의학회지
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• 제46권1호
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• pp.13-19
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• 2006

Generally known psittacosis or ornithosis is a disease of birds caused by the bacterium Chlamydia psittaci. Humans are accidential hosts and are most commonly infected from avian sources. It raises hepatitis or neurosis. As major outer membrane protein (MOMP) of Chlamydia psittaci has been known to play a role in the avoidance of host immune defenses, research on developing a Chlamydia vaccine has focused on the MOMP. In this study, the gene encoding the major outer membrane protein (MOMP) of the Chlamydia psittaci strain 6BC was cloned and expressed in Escherichia coli strain M-15. The recombinant DNA was cloned by fusion prokaryotic expression vector pQE30-GFPII. Expression of the recombinant protein was performed in E. coli and was induced by IPTG. The size of expressed recombinant protein is 74.220 kDa (MOMP, 43.260 kDa; GFP expression region, 30 kDa; $6{\times}His$ tag, 960Da). This protein was purified by using his-tagging-inclusion body. Recombinant protein was reconfirmed through ELISA test and western blot with antibody against pQE30-GFPII. It will be useful antibody development.

• 대한바이러스학회지
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• 제27권2호
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• pp.197-207
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• 1997

To investigate the NS4 region of JEV, NS4 cDNA of K94P05 (JEV strain isolated from Korea in 1994) was amplified by RT-PCR and analyzed by sequencing PCR product. Genomic size of NS4 was 1212bp and nucleotide sequence was compared with that of other JEV strains. Nucleotide homology between JaOAr582 and K94P05 was 91.1% and that between Beijing and K94P05 was 89.8%, respectively. But the nucleotide sequence of E region of JaOAr582 and K94P05 showed 97.0% homology and that of Beijing and K94P05 did 95.8% homology. NS4 protein was expressed as a form of fusion protein by a prokaryotic expression system. The induced fusion product showed a lower molecular weight than predicted size and remained insoluble. The NS4 protein might be cleavaged by E. coli protease. Concluding above results, high hydrophobicity of the NS4 protein supported the fact that this protein played a role as a membrane component and the poor nucleotide sequence conservativity among JEV strains suggested that this region might be important to adapt each viral growth environment.

• 폴리머
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• 제28권6호
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• pp.502-508
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• 2004

암을 치료하기 위한 양이온성 리포솜은 화학치료요법의 분야에서 발전되어 왔다. 양이온성 리포솜은 음이온성의 세포 표면에 정전기적 상호작용에 의해 결합이 된다. 본 연구의 목적은 음이온성 세포와 이온결합을 할 수 있는 양이온성 리포솜을 제조하는 것이다. 양이온성 리포솜은 지질인 1,2-디스테로일-sn-글리콜-3-포스포에탄올아민 (DSPE)과 양이온성 고분자인 폴리에틸렌이민 (PEI)을 그라프트 중합으로 합성된 물질로부터 제조하였다. 리포솜 표면의 이온 특성은 제타 포텐셜을 측정하여 확인하였다. 소 혈청 수용액에서 리포솜의 혈장 단백질 흡착 특성은 입자 크기와 탁도 변화를 측정하여 확인하였다. 완충 용액 속에서 리포솜 안정성을 예측하기 위하여 입자 크기를 7일 동안 상온에서 측정하였다. 양이온성 리포솜은 소 혈청 수용액에서 많은 양의 혈장 단백질이 흡착되었다. 이는 혈장 단백은 음전하를 가지고 있어서 양이온성 리포솜의 표면과 잘 붙기 때문이다. 양이온성 폴리에틸렌이민을 이용한 리포솜의 표면 변형은 소 혈청 수용액 내에서 단백질 흡착을 강화시킨다는 것을 예상하게 한다. 또한, 제조된 리포솜의 완충 용액 내에서 7일 동안 안정한 상태임을 관찰하였다.

• 한국식품영양과학회지
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• 제37권7호
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• pp.879-885
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• 2008

죽의 1회분량 당 총 열량은 148.8(대추죽)${\sim}294.1\;kcal$(잣죽)의 넓은 범위를 나타냈다. 음식별로 열량 함량을 보았을 때 잣죽이 294.1 kcal로 KDRI 한끼 기준량의 33.3%를 제공하여 잣죽의 열량이 가장 높았으며, 팥죽(285 kcal, 31.7%), 닭죽(249.5 kcal, 27.7%), 호박죽(243.8 kcal, 27.1%)의 순으로 열량이 높았다. 대추죽은 148.8 kcal로 가장 열량이 낮았다. 죽류의 1회분량 당 탄수화물 함량은 23.0(닭죽)${\sim}52.1\;g$(팥죽)의 범위의 수준이었으며, 평균 $34.7{\pm}9.1\;g$로 KDRI 한끼 탄수화물 권장량의 24.4%를 공급하였다. 죽류의 1회 분량 당 탄수화물 함량을 분석한 결과를 보았을 때 팥죽의 탄수화물 함량이 가장 많아 1회분량 당 탄수화물 함량이 52.1 g으로 KDRI 한끼 기준량에 36.7%를 제공하였으며, 다음으로 호박죽이 51.4 g으로 KDRI 한끼 기준량에 36.2%를 제공하였다. 1회분량 당 탄수화물 함량이 가장 작은 음식은 닭죽(23.0 g, 16.2%)으로 탄수화물 함량이 가장 낮았다. 음식별 탄수화물 영양밀도는 대추죽이 INQ 1.4로 가장 높아 다른 죽류보다 열량이 가장 낮은 반면 탄수화물 공급이 우수하였다. 그 다음으로 흰죽(INQ 1.3)과 호박죽(INQ 1.3) 순으로 탄수화물 영양밀도가 우수하였다. 반면 잣죽(INQ 0.6)과 닭죽(INQ 0.6)은 탄수화물 영양밀도가 낮아 다른 죽 종류보다 열량은 높은 반면 탄수화물의 함량이 낮았다. 죽류의 단백질 함량은 2.3(흰죽)${\sim}22.3\;g$(닭죽)의 범위 수준으로, 평균 $8.2{\pm}4.9\;g$로 KDRI 한끼 단백질 권장량의 25.6%를 공급하였다. 분석한 죽류 중 닭죽이 단백질 함량이 $22.3{\pm}0.03\;g$으로 가장 높았고, KDRI 한끼 단백질 권장량에 69.7%로 팥죽($12.6{\pm}0.14\;g$, 39.4%)과 콩죽($11.0{\pm}0.11\;g$, 34.4%) 등 두류를 첨가된 죽의 단백질 함량이 높았다. 단백질의 영양밀도가 가장 높은 음식 역시 닭죽(INQ 2.5)이 가장 높았고, 굴죽(INQ 1.5), 콩죽(INQ 1.3) 순으로 단백질 영양밀도가 우수하였고, 굴죽이 열량은 낮은 반면 단백질을 섭취할 수 있는 우수한 급원 음식임을 알 수 있다. 죽류의 지방 함량은 0.3(대추죽)${\sim}17.8\;g$(잣죽)의 범위였고, 평균 $4.8{\pm}4.5\;g$로 KDRI 한끼 지방 권장량의 21.1%를 공급하였다. 분석한 죽 중 지방함량이 가장 높은 음식은 잣죽으로 지방 함량이 $17.8{\pm}0.12\;g$으로 가장 높았고, KDRI 한끼 지방 권장량에 77.4%이었다. 콩죽($9.0{\pm}1.0\;g$, 39.1%)과 닭죽($7.6{\pm}0.17\;g$, 33.0%)의 지방함량이 높았다. 죽류 중 1회 분량 당 지방 함량이 가장 낮은 음식은 대추죽($0.3{\pm}0.01\;g$)으로 KDRI 한끼 지방 권장량에 1.3%를 공급하였다. 지방의 영양밀도는 잣죽(INQ 2.4), 흑임자죽(INQ 1.5), 콩죽(INQ 1.5) 순으로 지방 영양밀도가 우수하였다. 본 연구 결과를 통해 얻어진 한국 전통죽의 영양소 함량에 대한 자료가 국내외 한식당, 학교 및 산업체 급식 등 단체급식장에서 널리 활용되어 한국전통음식의 보급이 확대되는 기틀이 되기를 기대한다. 또한, 한국음식의 영양 성분에 대한 평균적인 data를 도출하기 위해서는 보다 확장해서 연구하고, 이에 대한 영양정보제공 등이 필요하다고 본다.

• 한국응용곤충학회지
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• 제30권2호
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• pp.144-149
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• 1991

국내에서 분리된 파밤나방 핵다각체병바이러스(Spodoptera exigua nuclear polyhedrosis virus: SeNPV)의 생화학적인 특성을 규명하기 위하여 몇가지 실험을 행하였다. SeNPV는 하나의 envelopeso내에 다수의 nucleocapsid가 존재하는 MNPV(multiple embeded NPV)형태였다. 다각체단백질은 분자량 30kb의 단일 band로 나타났으며, Spodoptera litura NPV와 Bombyx mori NPV의 다각체단백질 항체에 반응하여 뚜렷한 침강선을 형성하였다. 비리온 단백질을 은염색한 결과, 많은 수의 minor band들이 포함된 49개의 band로 나타났으며, 바이러스 DNA를 분리하여 여러종의 제한효소에 의한 대략적인 genome size는 약 110kb 였다.

• Bulletin of the Korean Chemical Society
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• 제29권10호
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• pp.1969-1972
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• 2008

In this study, horse spleen apoferritins were induced to form biominerals using up to 3000 Fe atoms per protein molecule. The morphology and crystallinity of the nanometer-sized biominerals formed in the ferritins were then analyzed using field emission-energy filtering-transmission electron microscopy (FE-TEM). The ferritins were found to have reconstitution yields of 60-70% in the experiments. The mean core size of the ferritins varied somewhat with protein concentrations, indicating that crystal growth in ferritins could be controlled via protein concentrations. The core mineral size increased with the amount of Fe used. Lattice fringes of the core, associated with good crystallinity, were found in all samples. The lattice fringe images of a single domain ferrihydrite mineral appeared frequently in the (011) planes (d-spacing of 0.246 nm) under [100] zone axis in all samples of this study. In addition, the lattice image occasionally revealed fringes corresponding to the (100) planes (d = 0.254 nm) from the [001] zone axis, indicating the characteristic pattern of hexagonal crystal lattice. Diffraction patterns in the minerals identified as ferrihydrite were fitted well into the space group of $P3_{1c}$.

• 한국축산식품학회지
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• 제31권4호
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• pp.543-550
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• 2011

Various concentrations of whey protein isolate (WPI), such as 0.1, 0.5, 1.0, 2.5, and 5.0%(w/v), containing 1.0%(w/v) eugenol were prepared by high speed homogenization to formulate nanoemulsions (NEs) and to investigate their antimicrobial activity. The results showed that particle size decreased according to increases in WPI concentration. Similarly, the ${\zeta}$-potential value was reduced to a negative charge when using WPI concentrations >0.1%(w/v). In contrast, no significant differences in particle size were observed during 1 mon of storage, except for the 0.1%(w/v) WPI NE. The ${\zeta}$-potential value depended on the increase in WPI concentration and storage duration, except for NE1 and NE5, suggesting that a low or high concentration of emulsifier was not effective for maintaining the droplet form of the eugenol NE. The results of an antibacterial effect investigation indicated that the growth of Escherichia coli was inhibited based on an increase in eugenol concentration in all NE formulations. Moreover, a membrane permeability study showed that total leakage content increased according to incubation time.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권1호
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• pp.52-55
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• 2002

Flow cytometric techniques were used to investigate cell size, protein content and cell cycle behavior of recombinant Saccharomyces cerevisiae strains producing human lysozyme (HLZ). Two different signal sequences, the native yeast $MF\alpha1$ signal sequence and the rat $\alpha-amylase$ signal sequence, were used for secretion of HLZ. The strain containing the rat $\alpha-amylase$ signal sequence showed a higher level of internal lysozyme and lower specific growth rates. Flow cytometric analysis of the total protein content and cell size showed the strain harboring the native yeast signal sequence had a higher total protein content than the strain containing the rat $\alpha-amylase$ signal sequence. Cell cycle analysis indicated that the two lysozyme producing recombinant strains had an increased number of cells in the $G_2+M$ phase of the yeast cell cycle compared with the host strain SEY2102.