The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Analysis of the NS4 Region of Japanese Encephalitis virus K94P05 Isolated from Korea

일본뇌염 바이러스 국내분리주 K94P05의 NS4 부위 분석

  • Kim, Eun-Jung (Div. of Arbovirus, Dept. of Viral Disease, National Institute of Health) ;
  • Nam, Jae-Hwan (Div. of Arbovirus, Dept. of Viral Disease, National Institute of Health) ;
  • Park, Yong-Kenun (Dept. of Microbiology, Korea University) ;
  • Cho, Hae-Wol (Div. of Arbovirus, Dept. of Viral Disease, National Institute of Health)
  • 김은정 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 남재환 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 박용근 (고려대학교 미생물학 연구실) ;
  • 조해월 (국립보건원 바이러스질환부 신경계바이러스과)
  • Published : 1997.12.30
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Abstract

To investigate the NS4 region of JEV, NS4 cDNA of K94P05 (JEV strain isolated from Korea in 1994) was amplified by RT-PCR and analyzed by sequencing PCR product. Genomic size of NS4 was 1212bp and nucleotide sequence was compared with that of other JEV strains. Nucleotide homology between JaOAr582 and K94P05 was 91.1% and that between Beijing and K94P05 was 89.8%, respectively. But the nucleotide sequence of E region of JaOAr582 and K94P05 showed 97.0% homology and that of Beijing and K94P05 did 95.8% homology. NS4 protein was expressed as a form of fusion protein by a prokaryotic expression system. The induced fusion product showed a lower molecular weight than predicted size and remained insoluble. The NS4 protein might be cleavaged by E. coli protease. Concluding above results, high hydrophobicity of the NS4 protein supported the fact that this protein played a role as a membrane component and the poor nucleotide sequence conservativity among JEV strains suggested that this region might be important to adapt each viral growth environment.

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