The Journal of Korean Society of Virology (대한바이러스학회지)

The Korean Society of Virology (대한바이러스학회)
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Analysis of the Three Dimensional Structure of Envelope Protein of the Japanes encephalitis virus Isolated in Korea

국내에서 분리된 일본뇌염 바이러스의 Envelope Protein의 3차구조 분석

  • Nam, Jae-Hwan (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
  • Chae, Soo-Lim (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
  • Kim, Eung-Jung (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
  • Yoon, Kyung-Sik (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
  • Lee, Ho-Dong (Division of Arboviruses, Department of Viral disease, National Institute of Health) ;
  • Koh, Hyun-Chul (Department of Pharmacology, College of Medicine, Hanyang University) ;
  • Cho, Hae-Wol (Division of Arboviruses, Department of Viral disease, National Institute of Health)
  • 남재환 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 채수림 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 김은정 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 윤경식 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 이호동 (국립보건원 바이러스질환부 신경계바이러스과) ;
  • 고현철 (한양대학교 의과대학 약리학교실) ;
  • 조해월 (국립보건원 바이러스질환부 신경계바이러스과)
  • Published : 1997.12.30
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Abstract

Three dimensional structures of envelope protein from Korean isolates and Nakayama-NIH strain of Japanese encephalitis virus (JEV) were deduced by a computer program (HyperChem 4.0 Chemplus 1.0) based on the data of the three dimentional structure of Tick-borne encephalitis virus. In the three dimensional structure of envelope protein, neutralizing epitope and T-helper cell recognition site of C-terminal region of Korean isolates were structually similar to those of Nakayama-NIH but the N-terminal region was not. Korean JE isolates were compared with Nakayama-NIH strain by using cross-neutralization antibody test. Neutralizing activities of Korean isolates derived from guinea pigs were higher than those of Nakayama-NIH strain against Korean isolates, although the polyclonal antibody titers of Nakayama-NlH showed 1:160 to 1:640 against Korean isolates. According to the results from three dimentional structures and cross-neutralization analyses, the antigenic difference between Korean JE isolates and Nakayama-NIH strain may be dependent on structural difference of envelope protein.

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