• 한국식품과학회지
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• 제10권1호
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• pp.83-87
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• 1978

한국산 호박씨중에서 지방(脂肪)을 추출하여 지방산(脂肪酸)의 종류를 조사하였고 또한 물에 가용(可溶)인 단백질(蛋白質) 및 불용성(不溶性) 단백질(蛋白質)을 분리(分離)하여 Disc 전기영동으로 단백질의 Pattern을 확인하고 아미노산 조성(組成)을 관찰하였다. 1. 호박씨의 일반성분중 지방은 46.5% 단백질 25.5%를 함유하고 있다. 2. 호박씨기름의 지방산(脂肪酸) 조성(組成)은 linoleic acid 51.74%, oleic acid 22.2%, palmitic acid 18.1%, stearic acid 7.84%로 이들 4가지를 주 지방산으로 하고 있다. 3. 호박씨 단백질(蛋白質)의 아미노산(酸) 조성(組成)은 tyrosine, glutamic acid, aspartic acid, leucine, glycine등을 다량(多量) 함유하고 있으나 함황(含黃) 아미노산(酸)은 함유(含有)하고 있지 않다. 4. 단백질의 Disc전기영동 결과 가용성(可溶性) 단백질(蛋白質)에서 13개의 band와 불용성(不溶性) 단백질에서 4개의 band가 분리 되었다.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1181-1181
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• 2001

Robinson with ${coworkers}^{1}$ have introduced two-state outer-neighbor bonding model to explain the anomalies of water. The studies on the properties of water as a function of temperature and pressure revealed that, unlike other ideas, all $H_2O$ molecules in liquid are tetrabonded. On the average they are forming two different bonding types. One type is the regular tetrahedral water-water bonding similar to that found in the ordinary ice Ih, whereas the other is a more dense nonregular tetrahedral bonding similar to that appearing in the ice II. The transformation between these two bonding forms is evidenced by FT-NIR experiment. The FT-NIR measurements were done for liquid water in the temperature range from $20^{\circ}C$ up to $80^{\circ}C$ in a wide extent of frequencies: 12 000 - 4000 $cm^{-1}$ /. Temperature dependent variations in the volume fraction of these two structures are directly related to the spectral changes. The absorbance variations are explored by means of the two-dimensional correlation spectroscopy (2DCOS), principal component analysis (PCA), curve fitting and second derivatives. The presence of the isosbestic points in a range of the combination and overtone transitions indicates that the experimental spectra are a superposition of two temperature independent components. One component of diminishing intensity with temperature increase, is assigned to a stronger hydrogen bonds occurred in the Ih type, whereas the second component showing an opposite behavior, one can attribute to a weaker H-bonds characteristic for the II type. The understanding of the hydrogen bonding network in the liquid water is very important in interpretation of the interaction between water and protein chain. The two-state model of water surrounding the protein surface could advance an understanding of the hydration process.

• 미생물학회지
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• 제51권4호
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• pp.435-439
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• 2015

THO/TREX 복합체는 전사 신장, mRNA 가공 및 방출, 그리고 유전체 안정성에 중요한 역할을 담당한다. 분열효모 Schizosaccharomyces pombe에서 THO/TREX 복합체의 한 구성요소인 THOC5의 이종상동체를 암호화하고 있는 SPBC 577.04 유전자를 찾아, 그것의 기능을 분석하였다. S. pombe thoc5 (spthoc5) 유전자는 생장과 mRNA의 방출에 필수적이지는 않지만, 결실돌연변이는 야생형에 비해 생장 결함을 보였고 $poly(A)^+$ RNA도 핵 안에 약간 축적되는 현상을 보였다. 또한 정상적인 기능을 가진 spThoc5-GFP 단백질은 주로 핵안에 존재하였다. Co-immunoprecipitation 분석에서 진화적으로 잘 보존된THO/TREX 복합체의 주요 구성인자인 Hpr1(THOC1)는 또 다른 구성인자인 Tho2 (THOC2) 뿐만 아니라 spThoc5와도 상호작용을 하였다. 이와 같은 결과들은 S. pombe의 Thoc5 상동체도 THO/TREX 복합체의 구성인자로 mRNA 방출에 관여하고 있음을 시사한다.

• The Plant Pathology Journal
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• 제28권2호
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• pp.197-201
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• 2012

Potyviruses express their RNA genomes through the production of polyproteins that are processed in host cells by three virus-encoded proteases. Soybean plants produce large amounts of protease inhibitors during seed development and in response to wounding that could affect the activities of these proteases. The in vitro activities of two of the proteases of Soybean mosaic virus (SMV) and Tobacco vein mottling virus (TVMV) were compared in the rabbit reticulocyte lysate in vitro translation system using synthetic RNA transcripts. Transcripts produced from SMV and TVMV cDNAs that included the P1 and helper component-protease (HC-Pro) coding regions directed synthesis of protein products that were only partially processed. Unprocessed poly-proteins were not detected from transcripts that included all of the P1, HC-Pro, P3 and portions of the cylindrical inclusion protein coding regions of either virus. Addition of soybean trypsin inhibitor to in vitro translation reactions increased the accumulation of the unprocessed polyprotein from TVMV transcripts, but did not alter the patterns of proteins produced from SMV. These experiments suggest that SMV-and TVMV-encoded proteases are differentially sensitive to protease inhibitors.

• Journal of Periodontal and Implant Science
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• 제36권1호
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• pp.265-272
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• 2006

Dental plaque, a biofilm consisting of more than 500 different bacterial species, is an etiological agent of human periodontal disease, It is therefore important to characterize interactions among periodontopathic microorganisms in order to understand the microbial pathogenesis of periodontal disease. Previous data have suggested a synergistic effect of tow major periodontal pathogens Porphyromonas gingivalis and Tannerella forsythia in the periodontal lesion. In the present study, to better understand interaction between P. gingivalis and T. forsythia, the coaggregation activity between these bacteria was characterized. The coaggregation activity was observed by a direct visual assay by mixing equal amount (1 ${\times}$ $10^9$)of T. forsythia and P. gingivaJis cells. It was found that the first aggregates began to appear after 5-10 min, and that the large aggregates completely settled within 1 h. Electron and epifluorescence microscopic studies confirmed cell-cell contact between two bacteria. The heat treatment of P. gingivalis completely blocked the activity, suggesting an involvement of a heat-labile component of P. gingivalis in the interaction. On the other hand, heat treatment of T. forsythia significantly increased the coaggregation activity; the aggregates began to appear immediately. The coaggregation activity was inhibited by addition of protease, however carbohydrates did not inhibit the activity, suggesting that coaggregation is a protein-protein interaction. The results of this study suggest that coaggregation between P. gingivalis and T. forsythia is a result of cell-cell physical contact, and that coaggregation is mediated by a heat-labile component of P. gingivalis and T. forsythia component that can be activated on heat treatment.

• 한국식품과학회지
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• 제20권2호
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• pp.133-139
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• 1988

액체 식품의 밀도를 예측할 수 있는 모델을 온도와 그 식품의 주요성분의 조성의 함수로 발전시키기 위하여 액체식품의 주요성분을 다음과 같이 water, protein, fat, carbohydrate, fiber, ash등 6가지 성분으로 분류하였고, 다시 세분하면 16가지의 주요성분을 분리 준비하여 그중 분말로 준비된 시료는 증류수를 사용하여 3종류의 서로 다른 농도의 현탁액을 만들어서 volumetric pycnometer를 사용하여 $0^{\circ}C$에서 $100^{\circ}C$의 온도범위에서 밀도를 측정하였다. 측정된 값으로부터 주어진 온도에서 각 순수성분의 밀도를 계산하였으며 이때 순수한 성분의 밀도는 온도의 증가에 따라 1차 함수의 관계로 감소한다는 사실을 알게 되었다. 계산된 밀도값으로부터 $0^{\circ}C$에서 $100^{\circ}C$의 온도범위에서 각 순수성분의 밀도 예측 모델의 계수를 OPT subroutine을 사용하여 결정하였다. 그리하여 Table 14 혹은 Table 15에 나타난 식을 활용하여 주어진 온도와 조성에서 액체식품의 밀도를 예측할 수 있을 것으로 사료된다.

• 기술사
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• 제28권5호
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• pp.12-19
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• 1995

한국산 구약 감자(elephant-foot=Amorphoph-alus Konjak K.Koch)의 이화학적 성분 조성을 조사하기 위하여 국산 생감자와 분말을 시료로 하여 외국산 정분과 같이 분석한 결과 생감자는 수분이 80.64%였고 고형분의 대부분은 당류, 단백질, 희분이고 섬유나 지방은 미량 존재하였다. 건조 분말 정제품인 정분은 당류 74.42-80.41%, 수분은 10% 전후였고 만난 함량은 한국산이 일본, 중국산보다 상당히 적었고 역시 가용성 유리당도 일본산이 높고 한국산이 낮았는데 구성당은 한국산은 Sucrose가 많은 대신 외국산은 Fructose, Glu-cose 함량이 높았다. 한국산 정분의 단백질 함량은 외국산보다 2배 많았고 이의 amino acid 조성은 Glutamic acid, Aspartic acid, Arginine 등이 총아미노산의 45%를 차지하고 Valine, Serine, Leucines, Glycine의 순서로 많았다. 정분의 무기물은 K가 많고 P>Na>Ca순으로 많게 함유하였고 chip의 정분 수율은 한국산이 61%, 중국산은 57.6%였으며 정분의 백도는 색도 측정치는 중국산이 약간 높았으나 육안으로는 별 차이가 없이 비슷하였다.

• Journal of Microbiology and Biotechnology
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• 제30권1호
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• pp.38-43
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• 2020

Hand, foot, and mouth disease (HFMD) is caused by enterovirus 71 (EV71) in infants and children under six years of age. HFMD is characterized by fever, mouth ulcers, and vesicular rashes on the palms and feet. EV71 also causes severe neurological manifestations, such as brainstem encephalitis and aseptic meningitis. Recently, frequent outbreaks of EV71 have occurred in the Asia-Pacific region, but currently, no effective antiviral drugs have been developed to treat the disease. In this study, we investigated the antiviral effect of salvianolic acid B (SalB) on EV71. SalB is a major component of the Salvia miltiorrhiza root and has been shown to be an effective treatment for subarachnoid hemorrhages and myocardial infarctions. HeLa cells were cultured in 12-well plates and treated with SalB (100 or 10 ㎍/ml) and 10⁶ PFU/ml of EV71. SalB treatment (100 ㎍/ml) significantly decreased the cleavage of the eukaryotic eIF4G1 protein and reduced the expression of the EV71 capsid protein VP1. In addition, SalB treatment showed a dramatic decrease in viral infection, measured by immunofluorescence staining. The Akt signaling pathway, a key component of cell survival and proliferation, was significantly increased in EV71-infected HeLa cells treated with 100 ㎍/ml SalB. RT-PCR results showed that the mRNA for anti-apoptotic protein Bcl-2 and the cell cycle regulator Cyclin-D1 were significantly increased by SalB treatment. These results indicate that SalB activates Akt/PKB signaling and inhibits apoptosis in infected HeLa cells. Taken together, these results suggest that SalB could be used to develop a new therapeutic drug for EV71-induced HFMD.

• Molecules and Cells
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• 제43권8호
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• pp.694-704
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• 2020

HslUV is a bacterial heat shock protein complex consisting of the AAA+ ATPase component HslU and the protease component HslV. HslV is a threonine (Thr) protease employing the N-terminal Thr residue in the mature protein as the catalytic residue. To date, HslUV from Gram-negative bacteria has been extensively studied. However, the mechanisms of action and activation of HslUV from Gram-positive bacteria, which have an additional N-terminal sequence before the catalytic Thr residue, remain to be revealed. In this study, we determined the crystal structures of HslV from the Gram-positive bacterium Staphylococcus aureus with and without HslU in the crystallization conditions. The structural comparison suggested that a structural transition to the symmetric form of HslV was triggered by ATP-bound HslU. More importantly, the additional N-terminal sequence was cleaved in the presence of HslU and ATP, exposing the Thr9 residue at the N-terminus and activating the ATP-dependent protease activity. Further biochemical studies demonstrated that the exposed N-terminal Thr residue is critical for catalysis with binding to the symmetric HslU hexamer. Since eukaryotic proteasomes have a similar additional N-terminal sequence, our results will improve our understanding of the common molecular mechanisms for the activation of proteasomes.

• Advances in Traditional Medicine
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• 제10권3호
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• pp.191-199
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• 2010

Acanthopanax Koreanum stem (AK) has been used in Korea as a tonic and sedative as well as a drug with ginseng like activities. The purpose of our present study was to investigate the effects of AK extract (AKE) and Eleutheroside E, major component of AKE on an exacerbated immune function through utilization of protein-energy malnutrition (PEM) diet by using forced swimming test (FST). The immobility time were significantly decreased in the AKE or Eleutheroside E-administrated group compared with the control group on the FST (P < 0.05). The level of blood parameters were not changed significantly. PEM-induced weight loss of mice was reduced by oral administration of 500 mg/kg AKE. AKE oral administration improved the nutritional status such as the food efficiency ratio and the adrenal gland weight. AKE treatment significantly increased the production of interferon (IFN)-$\gamma$ compared with unstimulated splenocytes but not interleukin (IL)-4. Eleutheroside E also significantly increased the IFN-$\gamma$ production but not IL-2 and IL-4 in T cell line, MOLT-4 cells. These results suggest that AKE and Eleutheroside E may influence to immune-enhancing through increasing the physical endurance capacity and immune cell activation.