Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Effects of fission yeast ortholog of THOC5 on growth and mRNA export in fission yeast

THOC5의 분열효모 이종상동체가 생장 및 mRNA export에 미치는 영향

  • Koh, Eun-Jin (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University) ;
  • Yoon, Jin Ho (Basic Science Research Institute, School of Biological Science and Chemistry, Sungshin Women's University)
  • 고은진 (성신여자대학교 생명과학.화학부 및 기초과학연구소) ;
  • 윤진호 (성신여자대학교 생명과학.화학부 및 기초과학연구소)
  • Received : 2015.11.25
  • Accepted : 2015.12.16
  • Published : 2015.12.31
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Abstract

THO/TREX complex plays an important role in transcriptional elongation, mRNA processing, nuclear RNA export, and genome stability. A fission yeast, Schizosaccharomyces pombe, SPBC577.04 gene encoding the ortholog of THOC5, a component of THO/TREX complex, was identified and characterized. The S. pombe thoc5 (spthoc5) is not essential for both growth and mRNA export, but deletion of the spthoc5 gene caused growth defect and slight accumulation of $poly(A)^+$ RNA in the nucleus. And the functional spThoc5-GFP protein is localized mainly in the nucleus. Co-immunoprecipitation analysis showed that the Hpr1(THOC1) protein, an evolutionally well-conserved component of THO/TREX complex, interacted with spThoc5 as well as Tho2(THOC2), another subunit of THO complex. These results suggest that S. pombe Thoc5 as a component of THO/TREX complex is also involved in mRNA export from the nucleus.

THO/TREX 복합체는 전사 신장, mRNA 가공 및 방출, 그리고 유전체 안정성에 중요한 역할을 담당한다. 분열효모 Schizosaccharomyces pombe에서 THO/TREX 복합체의 한 구성요소인 THOC5의 이종상동체를 암호화하고 있는 SPBC 577.04 유전자를 찾아, 그것의 기능을 분석하였다. S. pombe thoc5 (spthoc5) 유전자는 생장과 mRNA의 방출에 필수적이지는 않지만, 결실돌연변이는 야생형에 비해 생장 결함을 보였고 $poly(A)^+$ RNA도 핵 안에 약간 축적되는 현상을 보였다. 또한 정상적인 기능을 가진 spThoc5-GFP 단백질은 주로 핵안에 존재하였다. Co-immunoprecipitation 분석에서 진화적으로 잘 보존된THO/TREX 복합체의 주요 구성인자인 Hpr1(THOC1)는 또 다른 구성인자인 Tho2 (THOC2) 뿐만 아니라 spThoc5와도 상호작용을 하였다. 이와 같은 결과들은 S. pombe의 Thoc5 상동체도 THO/TREX 복합체의 구성인자로 mRNA 방출에 관여하고 있음을 시사한다.

Keywords

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