• Applied Chemistry for Engineering
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• v.18 no.5
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• pp.501-505
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• 2007

Organophosphorus hydrolase (OPH) expressed from recombinant Escherichia coli was used to biodegrade organophosphate insecticide coumaphos which has a very high toxicity in mammalian cells. To improve the productivity of OPH, the effects of nonionic surfactants (Tween 20, PEG 1000) and organic solvents, such as glycerol, propanol, and ethanol, were investigated in the strain culture. The maximum OPH was produced when the 0.25% of Tween 20 and 0.5% of glycerol were added to the medium. As the OPH obtained from disrupt-cell process by ultrasound treatment was used, the biodegradation efficiencies of 0.2, 0.5, 1.0 and 2.0 mM coumaphos were 100, 88, 84 and 78%, respectively. A novel method developed in this study could be applied to the biodetoxification technology in the contaminated region with various coumaphos concentration.

• Journal of The Korean Society of Grassland and Forage Science
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• v.14 no.2
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• pp.69-75
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• 1994

This experiment was canied out to study the effect of cutting frequencies (3, 4 and 5 cuts per year) and mineral nitrogen fertilization on dry matter yields, in order to estimate optimum level of fertilizing mineral nitrogen in orchardgrass(Dacry1is glomerafa L.). The results are as follows: 1. Dry matter yields were the highest in the 1st cut at 3 cutting frequency and in the 2nd cut at 4 and 5 cutting frequencies, and they shared 49.7, 37.0 and 37.0% of annual dry matter yield respectively. 2. When only phosphorus and potassium were applied, annual dry matter yields were between 9.4 and 11.5 tons per ha and the highest yield was observed at 3 cutting frequency. 3. Dry matter yield in relation to fertilization of mineral nitrogen was highly increased when more nitrogen was applied as 360 kg per ha compared to 240 kg per ha at 3 cutting frequency. At 4 and 5 cutting frequencies, increases in dry matter yield, to large extent, were showed at 120 and 150 kg of nitrogen per ha(30 kg Nlcutlha) compared to no application of nitrogen. The efficiencies of mineral nitrogen fertilization (kg DM/kg N) were 28.0, 22.7 and 20.6 kg dry matter yields per nitrogen(kg) respectively). 4. At 4 and 5 cutting frequencies, dry matter yields were reduced when 360 kg and 300 kg of mineral nitrogen per ha(90 kg and 60 kg Nlcutlha) were applied respectively. 5. Particularly at 4 and 5 cutting frequencies of this study, maximum marginal yields reached at 129.9 kg and 148.0 kg of fertilizing mineral nitrogen per ha, and economical borden of mineral nitrogen fertilization were between 222.4 and 250.0 kg and between 244.8 and 276.8 kg respectively. At the same cutting frequencies, the highest dry matter yields were obtained at 365.4 and 433.8 kg of fertilizing mineral nitrogen respectively.

• Proceedings of the Korean Society for Emotion and Sensibility Conference
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• 2002.11a
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• pp.85-96
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• 2002

Entering the period of Mass Customization, and with the help of the Digital Textile Printing technology, the purpose of this study is to propose two customer involved design process models, based on the Digital Textile Printing technology, and to compare their efficiencies and appropriateness with those of the existing design process model, which would lead us to discover the possibilities of Mass Customized design process. By the result obtained from the survey of 166 females in their twenties and thirties, the second Mass Customized design process model which gave most choices to prosumers was preferred the most, while the existing design process which gave no choice seemed to be preferred the least, and among the design elements of textile, color appeared to be the most significant influential factor in the preference of the clothing by the consumers. In summary, it appeared that every aspect of the society requires a shift in the process of the thought from Mass Production to Mass Customization at this point, and a guideline was made from the Mass Customized design process suggested in this study.

• Nuclear Engineering and Technology
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• v.33 no.4
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• pp.355-364
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• 2001

In order to evaluate and finally optimize the vitrification condition for combustible dry active waste (DAW), dust and gas generation characteristics were investigated for PE, cellulose, and mixed waste Tests were conducted by varying the operation variables such as melter configuration, excess oxygen amount, and waste feeding rate. Results showed that dust generation characteristics were affected by the operation parameters and the melter＇s configuration is the dominant one. For all tested DAWs, dust generation was reduced by increasing the waste feeding rate and the excessive oxygen amount in the melter. Among waste types, dust amount was decreased by the order of mixed wastes, PE, and cellulose. Other parameters such as temperature variation and operation time have also affected the dust generation. The optimum condition for the DAW vitrification was determined as the melter＇s configuration equipped for minimizing the waste dispersion with 20 kg/h of waste feeding rate and 100% of excessive oxygen supply. CO gas concentration in the off-gas was immediately influenced by the combustion state in the melter, but showed similar trend as the dust generation. For the NOx production during the vitrification process, thermal NOx, which is generated from the Post Combustion Chamber (PCC), rather than fuel NOx was assumed to be dominant. The gas cleaning of efficiencies of the PCC, wet scrubber, and Selective Catalytic Reduction system (SCR) were found to be high enough to keep the concentration of pollutants (CO, NOx, SOx, HCI) in the stack below their relevant emission limits.

• Journal of Microbiology and Biotechnology
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• v.22 no.3
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• pp.316-325
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• 2012

Xylanase has been used extensively in the industrial and agricultural fields. However, the low-yield production of xylanase from native species cannot meet the increasing demand of the market. Therefore, improving the heterologous expression of xylanase through basic gene optimization may help to overcome the shortage. In this study, we synthesized a high-GC-content native sequence of the thermostable xylanase gene xynB from Streptomyces olivaceoviridis A1 and, also designed a slightly AT-biased sequence with codons completely optimized to be favorable to Pichia pastoris. The comparison of the sequences' expression efficiencies in P. pastoris X33 was determined through the detection of single-copy-number integrants, which were quantified using qPCR. Surprisingly, the high GC content did not appear to be detrimental to the heterologous expression of xynB in yeast, whereas the optimized sequence, with its extremely skewed codon usage, exhibited more abundant accumulation of synthesized recombinant proteins in the yeast cell, but an approximately 30% reduction of the secretion level, deduced from the enzymatic activity assay. In this study, we developed a more accurate method for comparing the expression levels of individual yeast transformants. Moreover, our results provide a practical example for further investigation of what constitutes a rational design strategy for a heterologously expressed and secreted protein.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2004.03a
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• pp.262-270
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• 2004

Basic experiments were carried out using the THT-IV low-power Hall thruster to examine the influences of magnetic field shape and strength, and acceleration channel length on thruster performance and to establish guidelines for design of high-performance Hall thrusters. Thrusts were measured with varying magnetic field and channel structure. Exhaust plasma diagnostic measurement was also made to evaluate plume divergent angles and voltage utilization efficiencies. Ion current spatial profiles were measured with a Faraday cup, and ion energy distribution functions were estimated from data with a retarding potential analyzer. The thruster was stably operated with a highest performance under an optimum acceleration channel length of 20 mm and an optimum magnetic field with a maximum strength of about 150 Gauss near the channel exit and with some shape considering ion acceleration directions. Accordingly, an optimum magnetic field and channel structure is considered to exist under an operational condition, related to inner physical phenomena of plasma production, ion acceleration and exhaust plasma feature. A new Hall thruster was designed with basic research data of the THT-IV thruster. With the thruster with many considerations, long stable operations were achieved. In all experiments at 200-400 V with 1.5-3 mg/s, the thrust and the specific impulse ranged from 15 to 70 mN and from 1100 to 2300 see, respectively, in a low electric power range of 300~1300 W. The thrust efficiency reached 55 %. Hence, a large map of the thruster performance was successfully made. The thermal characteristics were also examined with data of both measured and calculated temperatures in the thruster body. Thermally safe conditions were achieved with all input powers.

• Transactions of the Korean hydrogen and new energy society
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• v.30 no.4
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• pp.303-311
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• 2019

Reversible solid oxide fuel cell (RSOFC) has become a prospective device for energy storage and hydrogen production. Many studies have been conducted around the world focusing on system efficiency improvement and realization. The system should have not only high efficiency but also a certain level of simplicity for stable operation. External waste steam utilization was proved to remarkably increase the efficiency at solid oxide electrolysis system. In this study, RSOFC system coupled with waste steam was proposed and optimized in term of simplicity and efficiency. Ejector for fuel recirculation is selected due to its simple design and high stability. Three system configurations using ejector for fuel recirculation were investigated for performance of design condition. In parametric study, the system efficiencies at different current density were analyzed. The system configurations were simulated using validated lumped model in EBSILON(R) program. The system components, balance of plants, were designed to work in both electrolysis and fuel cell modes, and their off-design characteristics were taken into account. The base case calculation shows that, the system with suction pump results in slightly lower efficiency but stack can be operated more stable with same inlet pressure of fuel and air electrode.

• Journal of Korean Society for Quality Management
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• v.47 no.1
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• pp.199-211
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• 2019

Purpose: The concept of quality is changing in the quality 4.0 era with the fourth industrial revolution in the world. This research aims to understand the characteristics of well-adapted companies against the quality 4.0 era and to improve the quality of clothing companies. Methods: We analyzed companies that responded well to the quality 4.0 era, especially Kutesmart using Quality 4.0 Matrix. We focused on the service process of Kutesmart and we suggested modified service process to improve quality. We also interviewed an expert to verify this process is valid. Results: We found that two types are classified of well-adapted companies against the quality 4.0 era. Especially, Kutesmart has built a smart factory and introduced new technologies like 3D scanner and big data analysis. However, Kutesmart has a weakness in post-purchase process like other clothing companies. Kutesmart could solve this problem with modular production method for damaged part of customer. Conclusion: This research can be used for better understanding of the characteristics of well-adapted companies against the quality 4.0 era and service process of Kutesmart that is custom clothing company for providing information for benchmarking in this industry. This study suggests that further empirical researches on the costs and the efficiencies of applying the new technologies are necessary.

• Applied Chemistry for Engineering
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• v.22 no.5
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• pp.486-489
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• 2011

In the present work, diazinon which is known as nondegradable and environmental toxic material was efficiently treated by the cell surface-displayed organophosphorus hydrolase (OPH) biocatalyst. The culture temperature of $25^{\circ}C$ culture temperature and the addition of 0.2 mM ethylenediamine tetraacetate (EDTA) were effective conditions for the production of recombinant OPH in Escherichia coli. 25 and 50 ppm diazinon were treated with removal rate of 4.5 and $7.2mg/g{\cdot}min$, respectively and with all over 90% removal efficiencies using recombinant cell lysates through ultrasonication disruption process. Thus, these experimental results could be utilized in environmental friendly biological treatment system for toxic chemicals such as diazinon.

• The Transactions of the Korean Institute of Power Electronics
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• v.27 no.1
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• pp.26-32
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• 2022

This paper proposes a power system that includes a 120k W fuel cell DC-DC converter (FDC) and 30 kW bidirectional DC-DC converter (BHDC) for a 150 kW fuel-cell vehicle. With a high DC link voltage of 800 V, the efficiency and power density of the power electronic components are improved. Through the modular design of FDC and BHDC, electric components are shared, resulting in reduced mass production costs. The switching frequency of 30 kHz of full SiC devices and optimal design of coupled inductor reduce the volume, achieving a power density of 8.3 kW/L. Furthermore, a synergetic operation strategy using variable limiter control of FDC and BHDC was proposed to efficiently operate the fuel cell vehicle considering the fuel cell stack efficiency according to the load. Finally, the performance of the prototype was verified by Highway Fuel Economy Driving Schedule testing, EMI test, and the linked operation between FDC and BHDC. The full load efficiencies of the FDC and BHDC prototypes are 98.47% and 98.74%, respectively.