• Title/Summary/Keyword: producing condition

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Stspension Culture of Betalain Producing Cell-Line and Characteristics of Hairy Root of Phytolacca esculenta V. Houtte (장록에서 유도한 Betalain 합성 세포주의 액체 배양과 모상근의 특성)

  • 양덕조;이성종윤길영강영희
    • KSBB Journal
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    • v.8 no.1
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    • pp.89-94
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    • 1993
  • Thc effect of light and phylohormonc on the belalaln synthesis was tested using the suspension culture of a rod(bclalaln produulng) cell line from Phytolacca esculenta V. Houtte. Betalain synthesis of rod-cell 1ine was strongly dependent on the irradiation of blue light, but induced by hormone, IAA and/or kinetin, in dark conditions. In a light condition, however, 2 mg/l of IAA increased the betalain content about 30% (per gram fresh weight), whereas more than 0.5mg/l of kinetin remarkably decreased. The hairy root derived from the same plant was also observed for the blue light-dependent pigmentation in the root-tips. When the hairy root grown in dark was transferred to the light condition, the accumulation of betalain was initiated after 12hours. Such pigmentation was completely inhabited by addition of a protein synthesis inhibitor.

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A Normative Analysis on Broiler Farm investment in Korea (육계 건물 및 시설에 대한 투자 분석)

  • 김정주
    • Journal of Animal Environmental Science
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    • v.3 no.1
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    • pp.49-56
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    • 1997
  • Most of Korean broiler farms are constructed with vinyl houses. Such types of broiler farms might be cheaper in terms of cost, but poor in terms of environment condition. According to enlargement of broiler farm size, high value production systems or automatic facilities are introduced in this field. However, investment may not always guarantee the profit of the management. Therefore, it is necessary to undertake the investment analysis before decision making for the investment. Under the assumption that 870 Pyoung(≒$3.3㎡) of a broiler farm within 1,740 Pyoung(≒3.3㎡) of land is built with the producing capacity of 50,000 heads of chicken in a time. The total investment is calculated to be 600 million won(1,000 won≒$1.1), and out of them 58.1% is provided by the owner, 31.6% from the government loan, 5.8% from government subsidy, and 5.3% from other agencies or banks. The expected profit of the broiler farm is 64.6 million won(1,000 won≒$1.1) per year. The IRR calculated is 0.0808 which means that the rate to profit of this project would be 8.1%. This also means that for this investment the interest rate of the capital provided should be less than 8.1% per year. Considering that the current opportunity cost of the own capital is 8.5% this project is not so attractive for those who is going to build broiler farms mainly with their own capital. In other words this project would not be profitable, unless the average interest rate of the loans provided for this project is less than 8.1% per year.

Study on the Production and the Culture Condition of Cholesterol Oxidase from Bacillus megterium SFO41 (Bacillus megaterium SFO41에 의한 Cholesterol Oxidase의 생산 및 최적 배양 조건)

  • 김관필;이창호;우철주;박희동
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.3
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    • pp.403-409
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    • 2001
  • A novel strain of SFO41 producing a large amount of cholesterol oxidase as an extracellular enzyme isolate from Korean salt fermented foods. The strain was identified as Bacillus megaterium based on morphological, cultural and physiological characteristics. Experiments were carried out to optimized the condition of cholesterol oxidase production using B. megaterium SFO41. B. megaterium SFO41 was shown to give the maximum yield of cholesterol oxidase in the medium containing 2.0% glucose, 0.5% yeast extract. 0.03% $MgSO_4{\cdot}7H_2O,\;0.02%\;K_2HPO_4,\;0.2%\;NH_4NO_3$ and 0.2% cholesterol. The optimum culture conditions, temperature, initial pH and agitation speed were $30^{\circ}C$, 7.0 and 150 rpm, respectively. The enzyme production reached a maximum level at 24 hr of cultivation (2.37 U).

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Optimization of Substract Concentration in Cell Production of Fungal Chitosan (균류키토산의 균체생산에서 기질농도 최적화에 관한 연구)

  • 김봉섭;서명교;노종수;이용희;이국의
    • Journal of Environmental Health Sciences
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    • v.29 no.3
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    • pp.72-78
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    • 2003
  • In the process of producing chitosan from crustacean shell, the use of excessive acid and alkli is causing the problems of environmental pollution and of production cost. In this study, one way to solve these problems is to cultivate fungi, then, to extract chitosan from the cell wall. By means of flask incubation and batch cultivation, the optimum cultivation conditions for mass production of continuous cultivation was found. Four strains used for the production of fungal chitosan were Gongronella butleri IF08080, Absidia coerulea IF05301, Rhizopus delemar IF04775, Mucor tuberculisporus IF09256. In flask incubation to select strain of producing much chitosan by means of experiment of the effect of initial pH, Absidia coerulea IFO 5301 had highest yield in FCs, 258.1 $\pm$ 47.3 mg/200 $m\ell$l at pH 6.5. In flask incubation under the optimum cultivation condition, temperature 27$^{\circ}C$, culture time 6days, glucose 2%, peptone 1%, (NH$_4$)$_2$ SO$_4$ 0.5%, $K_2$HPO$_4$ 0.1 %, Nacl 0.1 %, MgSO$_4$ㆍ7$H_2O$ 0.05%, CaCl$_2$ㆍ2$H_2O$ 0.01 %, the yield of DCW brought the highest yields. In batch bioreactor, the optimum cultivation condition was that cell suspended solution was 70 $m\ell$, aeration rate 0.5 l/min, agitation rate 800 rpm, culture time 36 hr. In continuous bioreactor, the optimum substrate flow rate was 4 ι/day.

Performance test of PVT-water system considering ambient air and circulating water temperature (외기 및 순환수 온도조건을 고려한 PVT-water 시스템의 성능실험)

  • Jeong, Yong-Dae;Nam, Yujin
    • KIEAE Journal
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    • v.15 no.5
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    • pp.83-88
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    • 2015
  • Purpose: Photovoltaic system is a technique for producing electrical power by utilizing solar energy, which can be used over 20 years with simple maintenance. However, in the case of photovoltaic systems, the energy conversion efficiency decreases as the surface temperature of module increases, compared with other renewable energy technologies. In this regard, PVT module can increase the energy utilization of a composite module as producing heat and electricity simultaneously by using solar energy. Currently, many researches have been promoting in order to develop a high efficiency PVT module in Korea. However, there are a few studies about the performance of the modules corresponding the shape of types and various heat exchangers of the PVT module. In this study, the electrical performance was measured by the change of the ambient temperature and the circulating water temperature using the fabricated PVT module. Method: Experiments were performed using a solar simulator. And this experiment was assumed that the weather condition was in each season, as winter, spring, autumn and summer. It was identified that the I-V curve associated with the change of the experimental conditions and confirmed the change in the electrical characteristics. Result: As a result, it was figured out that the surface temperature and the electrical performance changes in case conditions. The electrical performance was calculated in different temperature condition and the power production was confirmed by the change of module temperature.

Isolation and Characterization of Protease Producing B. amyloliquefaciens JH-35 from Food Waste (음식물 쓰레기로부터 Protease를 생산하는 B. amyloliquefaciens JH-35의 분리 및 특성)

  • Yoo, Jae Hong;Joo, Jin Ho;Kim, Sung Gug;Jang, In-Hwan
    • Korean Journal of Environmental Agriculture
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    • v.35 no.4
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    • pp.294-301
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    • 2016
  • BACKGROUND: Recent studies have described the importance of microbes and enzymes that can compost food waste. This study was carried out to improve production of protease of isolated microbes from food waste. METHODS AND RESULTS: Seven bacteria isolated from various sources were screened for protease production by adding skim milk into the agar medium. About 7 microbes producing protease were tested, and strain JH-35 showed the highest protease activity among them. The strain was identified as Bacillus amyloliquefaciens JH-35 based on morphological, cultural, physiological characteristics and 16S rRNA. In the fermentation experiment, the assay B. amyloliquefaciens JH-35 showed the highest protease activity in the condition of 1% glucose, 1.5% yeast extract and 0.2%$ K_2HPO_4$. The optimal condition of culture with temperature $35^{\circ}C$, initial pH of 7 and shaking speed of 200 rpm and 24 hr. CONCLUSION: The protease of the B. amyloliquefaciens JH-35 had its activity at pH 7 and the optimal culture time was 24 hr. Also, B. amyloliquefaciens JH-35 was high salt tolerance. Our results suggest that B. amyloliquefaciens JH-35 from food waste may have the potential to degrade protein and carbohydrate in food waste.

Deduction of Optimum Factors for Hydrogen Production from Organic Resources using a Continuous Reaction Process (연속반응공정을 이용한 유기성자원으로부터 수소생산을 위한 최적인자도출에 관한 연구)

  • Kim, Choong Gon;Shin, Hyun Gon
    • Journal of the Korea Organic Resources Recycling Association
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    • v.19 no.2
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    • pp.22-27
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    • 2011
  • This study was performed to find out the optimum condition for hydrogen production by changing mixture ratio from 3:7(food waste water : swine wastewater) without pre-treatment of food wastewater and swine wastewater using a continuous reaction process. It was confirmed that hydrogen generation according to pH is the highest in a condition of pH 5.5, and that the optimum pH for hydrogen production in case of mixing food wastewater with swine wastewater is 5.5 through this. Hydrogen generation according to HRT showed high hydrogen generation rate in case of 4 days rather than 3 days, and this involves largely in vitality of hydrogen producing bacteria according to variation of the HRT value, so it is judged that HRT also acts as an important factor to hydrogen producing bacteria. The organic removal efficiency recorded a removal efficiency of maximum TS 52%, VS 71%, TSS 83% and VSS 89% at the 6th day of operation, and it was confirmed that organic removal efficiency is possible even through an hydrogen production process.

The Optimization of Offset Printing Process for High Quality Color Reproduction(II);Platemaking and Presswork (고품질 색재현을 위한 오프셋인쇄 공정의 최적화에 관한 연구(II);제판과 인쇄공정을 중심으로)

  • Kim, Sung-Su;Kang, Sang-Hoon
    • Proceedings of the Korean Printing Society Conference
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    • 2007.11a
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    • pp.13-28
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    • 2007
  • Producing printing plate is essential progress to do offset printing. In this Film-less period, the more PS plate becomes extinct, the more the age of the Plate-Making of Exposure declines the place to stand. To do offset printing, the CTP (Computer to Plate) is taking a place of PS plate that covers speed, quality and economical problems. The biggest advantage of using CTP is that laser directly goes to the plate, thus there are no dust from the Plate-Making of Exposure. It is also theoretically able to print 200lpi${\sim}$300lpi as well as print 1751pi, because it has over 2400dpi resolution. The high quality printing could be available inside of the country, if printing machine keeps the optimum condition in offset printing. The CTP has many advantages, however there is a difficulty for the operators to preserve the equipment. The actual circumstance is that they cannot make a decision about how many dots need to be generated, and also it is necessary to know how to establish the setup at RIP on CTP to make the optimum condition output. If offset printing machine keeps the optimum condition, it would be able to print up to high quality printing however it is hard to comment what is the optimum condition for the printing machine. Anyone could say easy subjectively that machine is in the optimum condition, however it is objectively hard to estimate by number. In this research GATF / Plate Test target used to analyze the image and to make numerical value of the optimum condition of the CTP. It also used GATF / The sheep fed test printing 5.0 to know the density of the color representation, dot gain and gray balance for the optimum condition of the print machine. The purpose of this research is to represent the ISO 12647-2 which is the international standard with domestic printing equipments.

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The Effect of Frequency of Transcutaneous Electrical Nerve Stimulation (TENS) on Maximum Multi-finger Force Production

  • Karol, Sohit;Koh, Kyung;Kwon, Hyun Joon;Park, Yang Sun;Kwon, Young Ha;Shim, Jae Kun
    • Korean Journal of Applied Biomechanics
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    • v.26 no.1
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    • pp.93-99
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    • 2016
  • Objective: The purpose of this study was to investigate the effect of transcutaneous electrical nerve stimulation (TENS) treatment on maximum voluntary force (MVF) production. Methods: Ten healthy, young subjects (5 males and 5 females) participated in the study. MVF was recorded after a fifteen minute session of TENS stimulation under two conditions: low frequency (4 Hz) at maximum tolerable level and high frequency (110 Hz) at maximum tolerable level. TENS was provided simultaneously via self-adhesive electrodes placed on the finger pads of the index, middle, ring and little fingers. MVF was also recorded in a baseline condition with no TENS treatment. Data were collected in three different sessions on three consecutive days at the sametime of the day. Results: Results from the study show that on an average, MVF increasesby 25% for the index, middle and little fingers for TENS treatment with 4 Hz frequency as compared to the baseline condition. However, the 110 Hz condition did not result in a significantly different MVF than the baseline condition during individual finger pressing tasks. In addition, while producing MVF with all the four finger stogether, MVF was 30% higher for the 4 Hz conditionin comparison to the baseline condition, and 15% higher for the 110 Hz condition in comparison to the baseline condition respectively. Conclusion: The results suggest that stimulation ofafferent fibers onthe glabrous skinwith TENS could have a net facilitatory effect on the maximum motoroutput.

The Optimization of Offset Printing Process for High Quality Color Reproduction (II) - Platemaking and Presswork - (고품질 색재현을 위한 오프셋 인쇄공정의 최적화에 관한 연구(II) - 제판과 인쇄공정을 중심으로 -)

  • Kim, Sung-Su;Kang, Sang-Hoon
    • Journal of the Korean Graphic Arts Communication Society
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    • v.25 no.2
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    • pp.69-84
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    • 2007
  • Producing printing plate is essential progress to do offset printing. In this Film-less period, the more PS plate becomes extinct, the more the age of the Plate-Making of Exposure declines the place to stand. To do offset printing, the CTP (Computer to Plate) is taking a place of PS plate that covers speed, quality and economical problems. The biggest advantage of using CTP is that laser directly goes to the plate, thus there are no dust from the Plate-Making of Exposure. It is also theoretically able to print $200lpi{\sim}300lpi$ as well as print 1751pi, because it has over 2400dpi resolution. The high quality printing could be available inside of the country, if printing machine keeps the optimum condition in offset printing. The CTP has many advantages, however there is a difficulty for the operators to preserve the equipment. The actual circumstance is that they cannot make a decision about how many dots need to be generated, and also it is necessary to know how to establish the setup at RIP on CTP to make the optimum condition output. If offset printing machine keeps the optimum condition, it would be able to print up to high quality printing however it is hard to comment what is the optimum condition for the printing machine. Anyone could say easy subjectively that machine is in the optimum condition, however it is objectively hard to estimate by number. In this research GATF / Plate Test target used to analyze the image and to make numerical value of the optimum condition of the CTP. It also used GATF / The sheep fed test printing 5.0 to know the density of the color representation, dot gain and gray balance for the optimum condition of the print machine. The purpose of this research is to represent the ISO 12647-2 which is the international standard with domestic printing equipments.

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