Stspension Culture of Betalain Producing Cell-Line and Characteristics of Hairy Root of Phytolacca esculenta V. Houtte

장록에서 유도한 Betalain 합성 세포주의 액체 배양과 모상근의 특성

  • 양덕조 (충북대학교 자연과학대학 생물학과) ;
  • 이성종윤길영강영희 (충북대학교 자연과학대학 생물학과 충북대학교 자연과학대학 생물학과 연세대학교 이과대학 생물학과)
  • Published : 1993.03.01

Abstract

Thc effect of light and phylohormonc on the belalaln synthesis was tested using the suspension culture of a rod(bclalaln produulng) cell line from Phytolacca esculenta V. Houtte. Betalain synthesis of rod-cell 1ine was strongly dependent on the irradiation of blue light, but induced by hormone, IAA and/or kinetin, in dark conditions. In a light condition, however, 2 mg/l of IAA increased the betalain content about 30% (per gram fresh weight), whereas more than 0.5mg/l of kinetin remarkably decreased. The hairy root derived from the same plant was also observed for the blue light-dependent pigmentation in the root-tips. When the hairy root grown in dark was transferred to the light condition, the accumulation of betalain was initiated after 12hours. Such pigmentation was completely inhabited by addition of a protein synthesis inhibitor.

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