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Population Structure of the Gibberella fujikuroi Species Complex Associated with Rice and Corn in Korea

  • Kim, Ji-Hye;Kang, Mi-Ran;Kim, Hee-Kyoung;Lee, Seung-Ho;Lee, Theresa;Yun, Sung-Hwan
    • The Plant Pathology Journal
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    • v.28 no.4
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    • pp.357-363
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    • 2012
  • Several species belonging to the Gibberella fujikuroi species (Gf ) complex are commonly associated with rice and corn, not only causing serious diseases, but also producing fumonisins, a group of mycotoxins harmful to animals and humans. To characterize the population structure of the putative fumonisin-producing Gf complex in Korea, we obtained 276 candidate isolates from rice and corn harvested in 2009 and 2010 by diagnostic polymerase chain reaction with several specific primer sets. Phylogenetic trees were constructed using multilocus sequences (combined RPB2 and EF1A, totaling 1.6 kb) from these isolates. Among the 135 isolates from rice, F. fujikuroi (teleomorph: G. fujikuroi; 59.3%) and F. proliferatum (G. intermedia; 13.3%) were predominant, followed by F. concentricum (5.9%). Additionally, twenty-five (18.5%) rice isolates belonged in a distinct subclade of F. commune, a non-member of the Gf complex. In contrast, F. verticillioides was the most predominant species (38.3%) among the 141 corn isolates, and followed by F. fujikuroi (27.7%), F. proliferatum (14.9%), F. subglutinans (7.1%), and F. concentricum (2.8%). A single mating type (MAT1-1) was found predominantly among the Gf complex isolates examined. Possible distinct subclades were detected within the populations of F. fujikuroi and F. proliferatum; however, this needs further confirmation. This is the first reported population-level characterization of putative fumonisin-producing Gf complex associated with rice and corn in Korea.

Prevalence and Microbial Flora of Chicken Slaughtering and Processing Procedure

  • Seol, Kuk-Hwan;Han, Gi-Sung;Kim, Hyoun Wook;Chang, Oun-Ki;Oh, Mi-Hwa;Park, Beom-Young;Ham, Jun-Sang
    • Food Science of Animal Resources
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    • v.32 no.6
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    • pp.763-768
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    • 2012
  • This study has been performed to measure the prevalence and microbial flora on chicken slaughtering as well as the processing process from the months of October to November. Whole-chicken rinsing technique was used in order to analyze the incidence of microorganisms on chicken carcass at the stage before chilling (after evisceration), after chilling and after cutting. The swab technique was used on processing the processed samples, such as working plates and cutting knives. Brine and cooling water from four cooling tubs were taken from each processing processes and were used as samples. Furthermore, the matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) for whole cell fingerprinting in combination with a dedicated bioinformatic software tool was used to identify the isolated microorganisms. Of the tested samples and processes, brine ($4.50{\pm}0.64$ Log CFU/mL) and chicken carcass before chilling ($4.15{\pm}0.46$ Log CFU/mL) showed the highest population of microorganisms; the predominant microbial flora of them were Moellerella wisconsensis (54.84%), a member of the Enterobacteriaceae family, and Escherichia coli (60.36%), respectively. However, the predominant microbial flora of cut carcass was changed to Staphylococcus aureus (27.32%), which is a kind of pathogenic microorganism that can cause a food-borne illness. Therefore, the slaughtering and processing procedure of chicken are needed to be controlled more hygienically.

Biotype, serotype and antibiotics susceptibility of Yersinia enterocolitica isolated from zoo animals (동물원(動物園) 야생동물(野生動物)에서 분리(分離)한 Yersinia enterocolitica 의 생물형(生物型), 혈청형(血淸型) 및 항생제(抗生劑) 감수성(感受性))

  • Park, Seog-gee;Youn, En-sun;Kim, En-jung
    • Korean Journal of Veterinary Research
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    • v.34 no.1
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    • pp.85-91
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    • 1994
  • A study on the isolation of Yersiniae from the feces of wild animals(mammals 376, birds 19 and reptiles 13) in zoo and the biotype and serotype and susceptibility of 12 antibiotics was carried. Out of 408 animals, Yersiniae were isolated from 28 animals(6.9%). Of 28 isolates, 27 isolates(96.4%) were Y. enterocolitica and 1(3.6%) was Y. kristensenii. According to the species, 25(6.6%) of Y. enterocolitica and 1(0.3%) of Y. kristensenii were isolated from 376 mammals, 2(15.4%) of Y. enterocolitica from 13 reptiles but not isolated from 19 birds. According to the eating pattern, 8(5.2%) of Y. enterocolitica were isolated from 155 carnivora, 13(10%) of Y. enterocolitica from 123 herbivora, and 6(4.9%) of Y. enterocolitica and 1(0.8%) of Y. enterocolitica from 123 omnivora. Out of 27 isolates of Y. enterocolitica, all were biotype 1. And predominant serotype was 0:21(40.7%), and 0:5(37.0%), 0:6(11.1%), 0:1(3.7%), 0:9(3.7%) and untypable(3.7%). Yersiniae isolated from zoo animals were resistant to cephalothin(100%), ampicillin(96.4%), carbenicillin(96.4%) and tetracycline(14.3%) and streptomycin(3.6%) and susceptible to chloramphenicol(100%), colistin(100%), gentamicin(100%), kanamycin(100%), nalidixic acid(100%), polymyxin B(100%) and tobramycin(100%). The predominant multiple resistance pattern was Am-Cf-Cb(82.1%), and Am-Cf-Cb-Te(10.7%) and Am-Cf-Cb-Te-Sm(3.7%).

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Size-based Characteristics of Airborne Bacteria and Fungi Distributed in the General Hospital (종합병원의 실내공기에 분포하는 부유 세균과 진균의 입경별 종류와 특성)

  • Kim, Ki-Yeon;Lee, Chang-Rae;Kim, Chi-Nyon;Won, Jong Uk;No, Jaehoon
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.16 no.2
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    • pp.101-109
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    • 2006
  • The objective of this study is to provide fundamental data for pertinent management of indoor air quality through investigating the size-based characteristics of bioaerosol distributed in the general hospital. Measurement sites are main lobby, ICU, ward and laboratory and total five times were sampled with six-stage cascade impactor. Based on the result of this study, concentrations of airborne bacteria and fungi were the highest in main lobby as followed by an order of ward, ICU and laboratory. Concentrations of airborne bacteria was generally higher than those of airborne fungi and the ratio of indoor and outdoor concentration of both exceeded 1.0 in all the measurement sites of the general hospital. The predominant genera of airborne bacteria identified in the general hospital were Staphylococcus spp.(50%), Micrococcus spp.(15-20%), Corynebacterium spp.(5-20%), and Bacillus spp.(5-15%). On the other hand, the predominant genera of airborne fungi identified in the general hospital were Cladosporium spp.(30%), Penicillium spp.(20-25%), Aspergillus spp.(15-20%), and Alternaria spp.(10-20%). In regard to size distribution of bioaerosol, the detection rate was generally highest on 5 stage($1.1-2.1{\mu}m$) for airborne bacteria and on 1 stage(>$7.0{\mu}m$) for airborne fungi. Cleanliness of facilities in the general hospital and condition of HVAC system should be monitored regularly to prevent indoor air contamination by airborne microorganisms.

Studies on the Distribution of Polyphenols in the Parts of Quercus acutissima (상수리 나무중 Polyphenol 성분들의 분포에 관한 연구)

  • Moon, Ja Young;Cho, Sung Hye
    • Analytical Science and Technology
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    • v.11 no.6
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    • pp.478-484
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    • 1998
  • Distribution of polyphenolic compounds in oak tree (Quercus acutissima, three years old) collected from Forest Research Institute located in Kwang Leung, Kyeonggi-do, Korea, was investigated using chromatographic studies. Total 25 polyphenolic fractions were separated from an oak tree, of which 15, 11, 7, 7, and 4 were in leaf, stem, root, bark, and seed, respectively. Catechins are predominant compounds in the polyphenols and some flavonoids were also identified. Distribution of polyphenols was relatively different in each part. Polyphenols in all of the part studied, except leaf where polymer was not detected, were existed as polymeric, oligomeric, and monomeric forms. Relative contents of total polyphenols in Quercus acutissima were the highest in bark, followed by root, leaf, acorn, and stem. Monomeric polyphenols were the predominant compounds present in all of the part of the oak tree.

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A Clinical Report of 9 Cases of Congenital Thyroid Dysgenesis (선천성 갑상선 발육이상 9례(例)에 대한 보고)

  • Lee Samuel;Lee Seug-Zae;Lee Hyouk-Jin;Chon Seong-Eun;Park Yoon-Kyu
    • Korean Journal of Head & Neck Oncology
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    • v.10 no.2
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    • pp.206-211
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    • 1994
  • Congenital thyroid dysgenesis including agenesis, hypoplasia and ectopia is the predominant cause of permanent hypothyroidism. Of these, two thirds are due to an ectopic thyroid and about one third to complete thyroid agensis. From Jan. 1981 to Dec. 1992, authors experienced the 9 cases of congenital thyroid dysgenesis. Aberrent thyroid was 4 cases (44.4%), thyroid hemiagenesis with aberrent thyroid was 3 cases(33.3%) and thyroid hemiagenesis was 2 cases(22.2%). The most predominant site of aberrent thyroid is the base of tongue(85.7%). 7 patients(77.8%) revealed euthyroidism and among them, 4 patients showed elevated TSH level. Hypothyroidism was 2 patients (22.2%). 7 cases responded to thyroid suppressive therapy. 2 cases of lingual thyroid which did not responed to thyroid suppressive therapy underwent surgery and they have placed on thyroid suppressive therapy postoperatively.

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The Underwater Noise of Fishing Gears in Operation (망어구의 수중소음에 관한 연구)

  • 윤갑동
    • Journal of the Korean Society of Fisheries and Ocean Technology
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    • v.16 no.1
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    • pp.1-15
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    • 1980
  • An underwater recording system was designed to measure the sound spectra of the underwater noises produced by fishing gears in operation. Recorded were noi~es from three types of fishing gears: an anchovy set net, three anchovy boat seine net and a stern trawlnet. Acoustic analysis were made using a heterodyne analyzer, a digital frequency analyzer and a level recorder. The no;'e produced by the anchovy set net was found in the high frequency region of the onset of ambient noise spectrum with a slope of - 6 dB/octave. Here the ambient noise spectrum is higher, though similar in shape, than Knudsen spectrum, and is attributed to the breaking action of the coastal wave. Measured noise spectra during the fishing operations of the anchovy boat seine nets are attributed to the background noise of the sea in the presence of the fishing vessels. The frequency distribution of the noise was 20~5, 000 Hz in the case of two steel anchovy boat seiners, and 20-3,000 Hz in the case of the wooden anchovy boat seiner. The predominant frequency range was 250~350 Hz and maximum sound pressure level was 122 dB (re $1\muPa$) in the case of the steel boat and ] 17 dB in the case of the wooden boat. The noises produced by the trawl fishing gears are remarkably higher than the background noi~e in the presence of the fishing vessel. The frequency distribution of the noi~e was 20-6,300 Hz. The predominant frequency range was 100~200 Hz and maximum sound pressure level was 137 dB ( re $1\muPa$) . The noise spectra were not so much different from that caused by vibrations of the towing cable and the structure of the ground rope of the trawl net towed in an experimental tank.

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Vascular Variations in the Anterolateral Thigh Flap (전외측 대퇴부 피판의 혈관 변이)

  • Hwang, So Min;Kim, Min Wook;Lim, Kwang Ryeol;Jung, Yong Hui;Kim, Hyung Do;Kim, Hong Il
    • Archives of Reconstructive Microsurgery
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    • v.22 no.1
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    • pp.13-17
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    • 2013
  • Purpose: Although a fasciocutaneous perforator artery as a vascular pedicle has previously been shown to be predominant in the anterolateral thigh (ALT) flap, recent studies have shown that a myocutaneous perforator artery is predominant. We have attempted to attain a clinical understanding of the vascular variations in the ALT flap. Materials and Methods: We confirmed the origin of a perforator artery in 11 cases of ALT flap. We then reviewed the variations of the descending branch of the lateral femoral circumflex artery, known as the major origin of the flap, and the overall variations associated with an ALT flap. Results: In a total of 11 cases, there were 18 perforator arteries of the ALT flaps. In addition, there were 9 fasciocutaneous perforator arteries and another 9 myocutaneous ones. However, depending on the origin, there was great variability in the perforator artery. That is, there were unique variants in the descending branches of the lateral femoral circumflex artery, the major origin of the perforator artery, in 3 of the total 11 cases. Conclusion: Our results showed that (1) a perforator artery accounts for the high proportion of fasciocutaneous ones, (2) a perforator artery might not originate from the descending branch of the lateral femoral circumflex artery and (3) there might be unique variants in the descending branch. If considering this, surgeons would successfully elevate an ALT flap.

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The Effects of the Wave Propagation Path of Ground Vibration Induced by the Subway Train on the Reduction of Vibration Level (지하철 주변 지반진동의 전파경로가 진동레벨 감쇠에 미치는 영향)

  • Shin, Han-Chul;Cho, Sun-Kyu;Yang, Shin-Chu;Lee, Jong-Min
    • Journal of the Korean Society for Railway
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    • v.12 no.5
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    • pp.631-640
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    • 2009
  • In this paper, Numerical analysis was conducted to study on the reduction of ground vibration level induced by subway train. The analytical results indicated that the soil properties were the predominant factor affecting the ground vibration when the tunnel was located in the soil layer. On the other hand, the rock properties were the predominant one when the tunnel was located in the rock layer. The effects of the angle between the vertical line of vibration source and the receive point on the reduction of vibration level were also evaluated. There were little difference in the reduction of vibration level when the receive point was located within $30^{\circ}$. The vibration level, however, rapidly decreased when the angle between the two points was larger than $40^{\circ}$.

The Major Postharvest Disease of Onion and Its Control with Thymol Fumigation During Low-Temperature Storage

  • Ji, Sang Hye;Kim, Tae Kwang;Keum, Young Soo;Chun, Se-Chul
    • Mycobiology
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    • v.46 no.3
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    • pp.242-253
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    • 2018
  • Onion (Allium cepa L.) is one of the major vegetable crops in Korea that are damaged and lost by pathogenic fungal infection during storage due to a lack of proper storage conditions. The aim of this study was to determine an appropriate control measure using thymol to increase the shelf life of onions. To control fungal infections that occur during low-temperature storage, it is necessary to identify the predominant fungal pathogens that appear in low-temperature storage houses. Botrytis aclada was found to be the most predominant fungal pathogen during low-temperature storage. The antifungal activity of the plant essential oil thymol was tested and compared to that of the existing sulfur treatments. B. aclada growth was significantly inhibited up to 16 weeks with spray treatments using a thymol solution. To identify an appropriate method for treating onions in a low-temperature storage house, thymol was delivered by two fumigation treatment methods, either by heating it in the granule form or as a solution at low-temperature storage conditions (in vivo). We confirmed that the disease severity was reduced up to 96% by fumigating thymol solution compared to the untreated control. The efficacy of the fumigation of thymol solution was validated by testing onions in a low-temperature storage house in Muan, Jeollanam-do. Based on these results, the present study suggests that fumigation of the thymol solution as a natural preservative and fungicide can be used as an eco-friendly substitute for existing methods to control postharvest disease in long-term storage crops on a commercial scale.