• Journal of Bio-Environment Control
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• v.32 no.1
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• pp.8-14
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• 2023

This study was conducted to develop a precision automatic irrigation system in a nursery by considering the problems and improvements of manual and the conventional automatic irrigation system. The amount of irrigated water between the conventional automatic irrigation system and manual irrigation was 28.7 ± 4.4 g and 14.2 ± 4.3 g, respectively, and the coefficient of variation was less than 30%. However, the coefficient of variation of the conventional automatic irrigation system of 15%, was higher than that of manual irrigation of 30%. The irrigation test using the developed uniform irrigation system attached with the nozzle of a spray angle 80° and most highest uniformity was at height 600 mm. And coefficient of variation of the irrigation uniformity at the center part was within 20%, but irrigation amount of the edge part was lower 50% and over compared to the center part. As a result of a tomato grafting seedling cultivation test using the developed uniform irrigation system, the average plant height of seedling at the edge part was 28 mm but plant height at the center part was higher as 72 mm. Therefore, it was necessary to apply additional irrigation device at the edge part. The irrigation uniformity of the edge concentrated irrigation system was investigated that the irrigation amount of the edge part was irrigated by more than 50% compared with the center part, and coefficient of variation of the irrigation amount at the center part was less than 30%. As a result of a cucumber grafting seedling cultivation test using the edge concentrated irrigation system, the plant height of seedlings in the edge and central part of cultivation bed were 24% and 26%, respectively, so irrigation uniformity was higher then the uniform irrigation system. In order to improve the uniformity of seedlings, it is necessary to adjust the height of boom according to the growth of the seedling by installing a distance sensor in the overhead watering and boom irrigation system.

• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.526-535
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• 2021

Spatial sampling design plays an important role in GIS-based modeling studies because it increases modeling efficiency while reducing the cost of sampling. In the field of agricultural systems, research demand for high-resolution spatial databased modeling to predict and evaluate climate change impacts is growing rapidly. Accordingly, the need and importance of spatial sampling design are increasing. The purpose of this study was to design spatial sampling of paddy fields (11,386 grids with 1 km spatial resolution) in Korea for use in agricultural spatial modeling. A stratified random sampling design was developed and applied in 2030s, 2050s, and 2080s under two RCP scenarios of 4.5 and 8.5. Twenty-five weather and four soil characteristics were used as stratification variables. Stratification and sample allocation were optimized to ensure minimum sample size under given precision constraints for 16 target variables such as crop yield, greenhouse gas emission, and pest distribution. Precision and accuracy of the sampling were evaluated through sampling simulations based on coefficient of variation (CV) and relative bias, respectively. As a result, the paddy field could be optimized in the range of 5 to 21 strata and 46 to 69 samples. Evaluation results showed that target variables were within precision constraints (CV<0.05 except for crop yield) with low bias values (below 3%). These results can contribute to reducing sampling cost and computation time while having high predictive power. It is expected to be widely used as a representative sample grid in various agriculture spatial modeling studies.

• Korean Journal of Remote Sensing
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• v.38 no.6_1
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• pp.1445-1462
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• 2022

Compact Advanced Satellite 500-4 (CAS500-4), which is scheduled to be launched in 2025, is a mid-resolution satellite with a 5 m resolution developed for wide-area agriculture and forest observation. To utilize satellite images, it is important to establish a precision sensor model and establish accurate geometric information. Previous research reported that a precision sensor model could be automatically established through the process of matching ground control point (GCP) chips and satellite images. Therefore, to improve the geometric accuracy of satellite images, it is necessary to improve the GCP chip matching performance. This paper proposes an improved GCP chip matching scheme for improved precision sensor modeling of mid-resolution satellite images. When using high-resolution GCP chips for matching against mid-resolution satellite images, there are two major issues: handling the resolution difference between GCP chips and satellite images and finding the optimal quantity of GCP chips. To solve these issues, this study compared and analyzed chip matching performances according to various satellite image upsampling factors and various number of chips. RapidEye images with a resolution of 5m were used as mid-resolution satellite images. GCP chips were prepared from aerial orthographic images with a resolution of 0.25 m and satellite orthogonal images with a resolution of 0.5 m. Accuracy analysis was performed using manually extracted reference points. Experiment results show that upsampling factor of two and three significantly improved sensor model accuracy. They also show that the accuracy was maintained with reduced number of GCP chips of around 100. The results of the study confirmed the possibility of applying high-resolution GCP chips for automated precision sensor modeling of mid-resolution satellite images with improved accuracy. It is expected that the results of this study can be used to establish a precise sensor model for CAS500-4.

• Korean Journal of Environmental Agriculture
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• v.38 no.4
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• pp.321-331
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• 2019

BACKGROUND: Pinoxaden is the phenylpyrazoline herbicide developed by Syngenta Crop Protection, Inc. and marketed on 2006. The maximum residue levels for wheat and barley were set by import tolerance. Thus, Ministry of Food and Drug Safety (MFDS) official analytical method determining Pinoxaden residue was necessary in various food matrixes. Satisfaction of international guideline of CODEX (Codex Alimentarius Commission CAC/GL 40) and National Institute of Food and Drug Safety Evaluation-MFDS (2017) are additional pre-requirements for analytical method. In this study, liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was investigated to analyze residue of Pinoxaden (M4), which is defined as pesticide residue in Korea, in foods. METHODS AND RESULTS: Pinoxaden (M4) was extracted followed by acid digestion (2hr reflux with 1N HCl) and pH adjusting (pH 4-5 with 3% ammonium solution). To remove oil, additional clean-up step with hexane saturated with acetonitrile was required to high oil contained sample before purification. HLB cartridge and nylon syringe filter were used for purification. Then, samples were analyzed by LC-MS/MS using reserve phase column C₁₈. Five agricultural group representative commodities (mandarin, potato, soybean, hulled rice, and red pepper) were used to verify the method in this study. The liner matrix-matched calibration curves were confirmed with coefficient of determination (r²) > 0.99 at calibration range 0.002-0.2 mg/kg. The limits of detection and quantitation were 0.004 and 0.01 mg/kg, respectively, which were suitable to apply Positive List System (PLS). Mean average accuracies of pinoxaden (M4) were shown to be 74.0-105.7%. The precision of pinoxaden and its metabolites were also shown less than 14.5% for all five samples. CONCLUSION: The method investigated in this study was suitable to CODEX (CAC/GL 40) and National Institute of Food and Drug Safety Evaluation-MFDS (2017) guideline for residue analysis. Thus, this method can be useful for determining the residue in various food matrixes in routine analysis.

• Korean Journal of Environmental Agriculture
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• v.18 no.2
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• pp.185-190
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• 1999

Autotoxic substance(s) from alfalfa(Medicago sativa L.) plants reduces germination and growth of adjacent new alfalfa after alfalfa. The autotoxic chemical(s) in alfalfa are clearly unknown. Our objective was to improve the sensitivity of an alfalfa seedling bioassay for evaluating autotoxic leaf extracts. We determined critical extract concentrations that inhibit seed germination and seedling growth, compared two different culture media, and evaluated the effects of extract sterilization on the sensitivity of the assay, by using streptomycin and autoclaving method. An agar medium in petri plate gave better responses of germination and seedling growth to the extracts than using filter paper in the plate. On agar medium, the concentration of extract required to reach 50% inhibition of root length was 2.7 g $kg^{-1}$, and of germination and hypocotyl length were 3.8 and 9.9 g $kg^{-1}$, respectively. Leaf extracts with 100 ppm streptomycin stimulated germination significantly compared to Leaf extract alone but reduced root length of control by 43%. Root length was more sensitive to the autotoxin(s) than was germination or hypocotyl length. These results suggest that agar medium mixed with extract and sterilization by autoclaving could be improved the consistency and precision of bioassay, and that root length was the best parameter of autotoxic effect of alfalfa leaf extract.

• Korean Journal of Environmental Agriculture
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• v.32 no.3
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• pp.207-213
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• 2013

BACKGROUND: This study was conducted to develop analytical method with reproducibility, accuracy and applicability to agricultural products than the existing methods. METHODS AND RESULTS: Mean recoveries of thidiazuron ranged from 89.2 to 91.2 in hulled rices, 87.2 to 92.1 in peppers, from 76.4 to 86.9 in potatoes, from 91.2 to 95.7 in watermelons, from 86.5 to 88.5 in kiwi fruits, and from 89.5 to 94.0 in grapes, with less than 10% of relative standard deviations. In addition, the limit of quantitation was set to be 0.05 mg/kg and there were no interfering peaks in integrating the thidiazuron peak. CONCLUSION(S): These results represent that the enhanced analytical method has reliable accuracy, precision, selectivity, and sensitivity.

• Journal of Mushroom
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• v.14 no.4
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• pp.249-252
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• 2016

Timing, place, and the high-tech industry to overcome barriers to the introduct of the so-called "smart agriculture". The core factors of precision agriculture, including temperature, humidity, location information, and real summary information, are all significant for ICT controlling technique. The system is a four-season container allowing mushroom product, humidity, illumination, and factors such as carbon dioxide were regulated to create the optimal environment for mushroom cultivation. Hericium erinaceum (also called Lion's Mane Mushroom, Bearded Tooth Mushroom, or Bearded Tooth Fungus) is an edible and medicinal mushroom in the tooth fungus group. During approximately 40 days of incubation, 1.4 kg of oak sawdust was used as the test medium. The humidity, temperature, and $CO_2$ density were maintained at >85-95%, $18-20^{\circ}C$, and 700-1,000 ppm, respectively, in the container type culture system. The time for primordium formation was observed to be 4-7 days. The amount of fruitbody production was 74-95 g per bag. This technique will be used to establish a new community support program, in conjunction with international movement, domestic sales and exports are also planned.

• Korean Journal of Environmental Agriculture
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• v.31 no.3
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• pp.277-285
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• 2012

BACKGROUND: For safety evaluation of imported agri-food in Korea, the multiresidue analysis method was establised for unregistered organophosphorus pesticides, aspon, chlorthion, chlorthiophos, crotoxyphos, demeton-O, demeton-S, demeton-S-methyl, dioxathion, heptenophos, iodofenphos, leptophos, methyl-trithion, propetamphos and sulfotep. METHODS AND RESULTS: The used method for multiresidue analysis in brown rice and orange used as representative samples of imported agri-food was the official method of Korean Food and Drug Administration. The results of validation test of 13 organophosphorus pesticides except crotoxyphos for multiresidue analysis method are compared to the criteria such as specificity, linearity, accuracy, precision and limit of quantification. CONCLUSION: The used method for multiresidue analysis of unregistered 13 organophosphorus pesticides except crotoxyphos in Korea can surely be used as an official method for routine analysis of imported agri-food.

• Korean Journal of Environmental Agriculture
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• v.37 no.1
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• pp.57-65
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• 2018

BACKGROUND: An analytical method was developed using HPLC-UVD/MS to precisely determine the residue of flusulfamide, a benzenesulfonamide fungicide used to inhibit spore germination. METHODS AND RESULTS: Flusulfamide residue was extracted with acetone from representative samples of five raw products which comprised apple, green pepper, Kimchi cabbage, hulled rice, and soybean. The extract was diluted with large volume of saline water and directly partitioned into dichloromethane to remove polar co-extractives in the aqueous phase. For the hulled rice and soybean samples, n-hexane/acetonitrile partition was additionally employed to remove non-polar lipids. The extract was finally purified by optimized Florisil column chromatography. On an octadecylsilyl column in HPLC, flusulfamide was successfully separated from co-extractives of sample, and sensitively quantitated by ultraviolet absorption at 280 nm with no interference. Accuracy and precision of the proposed method was validated by the recovery experiment on every crop sample fortified with flusulfamide at 3 concentration levels per crop in each triplication. CONCLUSION: Mean recoveries ranged from 82.3 to 98.2% in five representative agricultural commodities. The coefficients of variation were all less than 10%, irrespective of sample types and fortification levels. Limit of quantitation (LOQ) of flusulfamide was 0.02 mg/kg as verified by the recovery experiment. A confirmatory method using LC/MS with selected-ion monitoring technique was also provided to clearly identify the suspected residue.

• Korean Journal of Environmental Agriculture
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• v.36 no.4
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• pp.269-278
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• 2017

BACKGROUND: The objective of this study was to evaluate screening method of residual multi pesticides in dead birds by Orbitrap high resolution mass spectrometry (HRMS) to identify the cause of death for birds. METHODS AND RESULTS: Extraction and clean-up method of residual pesticides in liver of dead birds was used QuEChERS (Quick Easy Cheap Effective Rugged and Safe) and method validations was conducted using liquid chromatography and gas chroamtography with triple-quadrupole mass spectrometer (LC/MS/MS and GC/MS/MS) Also, we were evaluated screening method for the determination of residual pesticides in liver of dead birds by LC and GC Orbitrap Mass Spectrometry. Results of method validations, Correlation coefficients of the matrix matched calibration curves were >0.978, and the method detection limits (MDLs) and limits of quantitation (LOQ) were 2.8~72.1 ng/g (18.4 ng/g on average) and 9.0~230 ng/g (58.5 ng/g on average). The accuracy ranged from 69.1%to 130% (103% on average), and the precision values were less than 14.8%(3.8%on average). The screening of residual pesticides in liver of dead birds by LC and GC Orbitrap HRMS was detected monocrotophos, carbofuran, carbosulfan, deltametrin, benfuracarb, carbofuran, phosphamidon, prochloraz in investigated samples. CONCLUSION: This results showed that accurate mass were extraction of residual pesticides in dead birds by Orbitrap HRMS. It suggested that this screening method is applicable to the residual pesticide analysis for the cause of death as a main tool.