DOI QR코드

DOI QR Code

Residual Multi Pesticides Screening of Dead Birds by Orbitrap High Resolution Mass Spectrometry

오비트랩 고분해능 질량분석기를 이용한 폐사 조류 중 다성분 잔류 농약 스크리닝 기법

  • Lee, Doo-Hee (Environmental Measurement & Analysis Center, Environmental Infrastructure Research Department, National Institute of Environmental Research) ;
  • Kim, Bo-Kyong (Environmental Measurement & Analysis Center, Environmental Infrastructure Research Department, National Institute of Environmental Research) ;
  • Wang, Seung-Jun (Environmental Health Research Division, Environmental Health Research Department, National Institute of Environmental Research) ;
  • Son, Ki-Dong (Environmental Health Research Division, Environmental Health Research Department, National Institute of Environmental Research) ;
  • Jung, Hyen-Mi (Environmental Infrastructure Research Department, National Institute of Environmental Research) ;
  • Choi, Jong-Woo (Environmental Measurement & Analysis Center, Environmental Infrastructure Research Department, National Institute of Environmental Research)
  • 이두희 (국립환경과학원 환경기반연구부 환경측정분석센터) ;
  • 김보경 (국립환경과학원 환경기반연구부 환경측정분석센터) ;
  • 왕승준 (국립환경과학원 환경건강연구부 환경보건연구과) ;
  • 손기동 (국립환경과학원 환경건강연구부 환경보건연구과) ;
  • 정현미 (국립환경과학원 환경기반연구부) ;
  • 최종우 (국립환경과학원 환경기반연구부 환경측정분석센터)
  • Received : 2017.09.27
  • Accepted : 2017.11.08
  • Published : 2017.12.31

Abstract

BACKGROUND: The objective of this study was to evaluate screening method of residual multi pesticides in dead birds by Orbitrap high resolution mass spectrometry (HRMS) to identify the cause of death for birds. METHODS AND RESULTS: Extraction and clean-up method of residual pesticides in liver of dead birds was used QuEChERS (Quick Easy Cheap Effective Rugged and Safe) and method validations was conducted using liquid chromatography and gas chroamtography with triple-quadrupole mass spectrometer (LC/MS/MS and GC/MS/MS) Also, we were evaluated screening method for the determination of residual pesticides in liver of dead birds by LC and GC Orbitrap Mass Spectrometry. Results of method validations, Correlation coefficients of the matrix matched calibration curves were >0.978, and the method detection limits (MDLs) and limits of quantitation (LOQ) were 2.8~72.1 ng/g (18.4 ng/g on average) and 9.0~230 ng/g (58.5 ng/g on average). The accuracy ranged from 69.1%to 130% (103% on average), and the precision values were less than 14.8%(3.8%on average). The screening of residual pesticides in liver of dead birds by LC and GC Orbitrap HRMS was detected monocrotophos, carbofuran, carbosulfan, deltametrin, benfuracarb, carbofuran, phosphamidon, prochloraz in investigated samples. CONCLUSION: This results showed that accurate mass were extraction of residual pesticides in dead birds by Orbitrap HRMS. It suggested that this screening method is applicable to the residual pesticide analysis for the cause of death as a main tool.

Keywords

References

  1. Alder, L., Steinborn, A., & Bergelt, S. (2011). Suitability of an orbitrap mass spectrometer for the screening of pesticide residues in extracts of fruits and vegetables. Journal of AOAC International, 94(6), 1661-1673. https://doi.org/10.5740/jaoacint.SGEAlder
  2. Bletsou, A. A., Jeon, J., Hollender, J., Archontaki, E., & Thomaidis, N. S. (2015). Targeted and non-targeted liquid chromatography-mass spectrometric workflows for identification of transformation products of emerging pollutants in the aquatic environment. TrAC Trends in Analytical Chemistry, 66, 32-44. https://doi.org/10.1016/j.trac.2014.11.009
  3. Cervera, M. I., Portoles, T., Pitarch, E., Beltran, J., & Hernandez, F. (2012). Application of gas chromatography time-of-flight mass spectrometry for target and non-target analysis of pesticide residues in fruits and vegetables. Journal of Chromatography A, 1244, 168- 177. https://doi.org/10.1016/j.chroma.2012.04.063
  4. Farre, M., Pico, Y., & Barcelo, D. (2014). Application of ultra-high pressure liquid chromatography linear iontrap orbitrap to qualitative and quantitative assessment of pesticide residues. Journal of Chromatography A, 1328, 66-79. https://doi.org/10.1016/j.chroma.2013.12.082
  5. Garcia-Reyes, J. F., Hernando, M. D., Molina-Diaz, A., & Fernandez-Alba, A. R. (2007). Comprehensive screening of target, non-target and unknown pesticides in food by LC-TOF-MS. TrAC Trends in Analytical Chemistry, 26(8), 828-841. https://doi.org/10.1016/j.trac.2007.06.006
  6. Han, L., Matarrita, J., Sapozhnikova, Y., & Lehotay, S. J. (2016). Evaluation of a recent product to remove lipids and other matrix co-extractives in the analysis of pesticide residues and environmental contaminants in foods. Journal of Chromatography A, 1449, 17-29. https://doi.org/10.1016/j.chroma.2016.04.052
  7. Hernandez, F., Ibanez, M., Portoles, T., Cervera, M., Sancho , J. & Lopez, F. (2015). Advancing towards universal screening for organic pollutants in waters, Journal of Hazardous Materials, 282, 86-95. https://doi.org/10.1016/j.jhazmat.2014.08.006
  8. Huerta, B., Jakimska, A., Gros, M., Rodriguez-Mozaz, S., & Barcelo D. (2013). Analysis of multi-class pharmaceuticals in fish tissues by ultra-high-performance liquid chromatography tandem mass spectrometry, Journal of Chromatography A, 1288, 63-72. https://doi.org/10.1016/j.chroma.2013.03.001
  9. Jang, J. H., Bong, Y. H., Kim, D. G., Kim, M., Chung, G. S., & Son, S. W. (2010). Analysis of residual pesticides in dead wild birds and other animals during 2008- 2009 in Korea. Korean Journal of Veterinary Research, 50(3), 197-203.
  10. Jeon J. H., Park, N. R., &Lee, S. H. (2016). Application of target, suspect, nontarget screening metholds based on high resolution mass spectrometry for the identification of micropollutants and their transformation products in aquatic evironments: A review. Journal of Korean Society for Environmental Analysis. 19(4), 225-245.
  11. Kim, M., Yun, S. J., Kim, D. G., Bong, Y. H., Kim, H. J., Jang, J. H., & Chung, G. S. (2008). Determination of pesticides in dead wild birds in Korea. Korean Journal of Veterinary Research, 48(2), 131-137.
  12. Krauss, M., Singer, H., & Hollender, J. (2010). LC high resolution MS in environmental analysis: from target screening to the identification of unknowns. Analytical and Bioanalytical Chemistry, 397(3), 943-951. https://doi.org/10.1007/s00216-010-3608-9
  13. Kwon, Y. K., Yun, S. J., & Kim, K. S. (2003). Parathion Poisoning in the White-napped Cranes. Korean Journal of Veterinary Public Health, 27(2), 83-88.
  14. Lehotay, S. J., Tully, J., Garca, A. V., Contreras, M., Mol, H., Heinke, V., Anspach, T., Lach, G., Fusseli, R., Mastovska, K., & Poulsen, M. E. (2007). Determination of pesticide residues in foods by acetonitrile extraction and partitioning with magnesium sulfate: collaborative study, Journal of AOAC International, 90(2), 485-520.
  15. Meng, C. K., Zweigenbaum, J., Frst, P., & Blanke, E. (2010). Finding and confirming nontargeted pesticides using GC/MS, LC/quadrupole-time-of-flight MS, and databases. Journal of AOAC International, 93(2), 703- 711.
  16. Ock, H. S. (2009). Developmental trend of analytical methods for pesticide residues. The Korean Journal of Pesticide Science, 13(4), 336-348.
  17. Park, B. J, Choi, J. H., Lee, B. M., Im, G. J., Kim, C. S., & Park, K. H. (1998). Decomposition rate of iprobenfos, isoprothiolane, and diazinon by some environmental factors in aqueous. The Korean Journal of Pesticide Science, 2(2), 39-44.
  18. Park, J. W., Kim, A. K., Kim, J. P., Lee, H. H., Park, D. W., Moon, S. J., Jang, T. K., Ha, D. R., & Seo, K. W. (2014). Multi-residue analysis of pesticides using GC-TOF/MS, ECD, NPD with QuECHERS sample preparation. The Korean Journal of Pesticide Science, 18(4), 278-295. https://doi.org/10.7585/kjps.2014.18.4.278
  19. Shaikh, H., Memon, N., Bhanger, M. I., & Nizamani, S. M. (2014). GC/MS based non-target screening of organic contaminants in river Indus and its tributaries in Sindh (Pakistan). Pakistan Journal of Analytical & Environmental Chemistry, 15(1), 42-65.
  20. Zedda, M., & Zwiener, C. (2012). Is nontarget screening of emerging contaminants by LC-HRMS successful? A plea for comound libraries and computer tools. Analytical & Bioanalytical Chemistry, 403(9), 2493-2502. https://doi.org/10.1007/s00216-012-5893-y