• Journal of information and communication convergence engineering
• /
• v.21 no.4
• /
• pp.322-328
• /
• 2023

The number of senior citizens with large bowel obstruction is steadily growing in Korea. Plain radiography was used to examine the severity and treatment of this phenomenon. To avoid examiner subjectivity in radiography readings, we propose an automatic segmentation method to identify fluid-filled areas indicative of large bowel obstruction. Our proposed method applies the Hough transform to locate suspicious areas successfully and applies the possibilistic fuzzy c-means unsupervised learning algorithm to form the target area in a noisy environment. In an experiment with 104 real-world large-bowel obstruction radiographs, the proposed method successfully identified all suspicious areas in 73 of 104 input images and partially identified the target area in another 21 images. Additionally, the proposed method shows a true-positive rate of over 91% and false-positive rate of less than 3% for pixel-level area formation. These performance evaluation statistics are significantly better than those of the possibilistic c-means and fuzzy c-means-based strategies; thus, this hybrid strategy of automatic segmentation of large bowel suspicious areas is successful and might be feasible for real-world use.

• Food Science of Animal Resources
• /
• v.31 no.6
• /
• pp.851-857
• /
• 2011

This study was conducted to develop a real time reverse transcriptase-PCR (RT-PCR) method for the detection of viable Enterobacteriaceae in milk using primers based on the genes of ribosomal proteins S11 and S13 and to determine effects of heating and subsequent treatments on the threshold cycle (Ct) of the real time RT-PCR. Total RNA was isolated from 17 strains of bacteria including 11 strains of Enterobacteriaceae suspended in milk using a modified Tri reagent method. SYBR Green Master Mix was added to the RNA and the mixture was subjected to the real time RT-PCR. The Cts of eleven type strains of the Enterobacteriaceae in milk ($10^7$ cells) in the real time RT-PCR ranged from 21.5 to 24.6. However, the Cts of Pseudomonas fluorescens, Acinetobacter calcoaceticus, and three gram-positive bacteria were more than 40. The real time RT-PCR detected as low as $10^3$ cells in agarose gel electrophoresis. The Cts increased from 22.0 to 34.2 when milk samples contaminated with Escherichia coli ($10^7$ cells/mL) were heated at $65^{\circ}C$ for 30 min. In addition, subsequent incubation at $37^{\circ}C$ for 6 and 24 h increased the Cts further up to 36.2 and 37.2, respectively. Addition of RNase A to the bacterial suspension obtained from the heated milk and subsequent incubation at $37^{\circ}C$ for 1 h increased the Cts to more than 40. The results of this study suggests that pretreatment of bacterial cells heated in milk with RNase A before RNA extraction might enhance the ability to differentiate between viable and dead bacteria using real time RT-PCR.

• Journal of The Korean Association of Information Education
• /
• v.25 no.5
• /
• pp.825-834
• /
• 2021

This study analyzed learners' satisfaction and in-depth interviews with teachers after operating a software practical curriculum as a real-time video class for fifth and sixth graders in elementary school. The correlation between learner's presence, class overall, interaction, and real-time video class satisfaction showed that the positive correlation between presence, class overall, interaction, and satisfaction with real-time video classes was somewhat high. There were some differences in the real-time video class participation environment and real-time video class satisfaction, but it was not found to be statistically significant. In the case of teachers, it was difficult to respond to problems occurring in each student's individual environment, interactions between students, and individual feedback problems for a sluggish student. To solve this problem, opinions such as preliminary guidance and verification of real-time video class connection environment, error support for sluggish students and individual class participation environment, and feedback on individual tasks using LMS were suggested.

• Information Systems Review
• /
• v.17 no.1
• /
• pp.117-140
• /
• 2015

Cloud Computing has drawn attention as one of 10 IT strategic technology trends and has various advantages such as cost reduction and enhancing business flexibility. However, corporations hesitate to adopt the service because of unexpected risks. Especially compared to large firm, medium and small ones use public cloud that security risk is high. Meanwhile, real option strategy has drawn attention as the method to hedge uncertainty in IT projects. Therefore, in this study causal relationships among technical, security, relational, and economic risks of cloud service will be investigated. Eventually, this study investigates how those risks influence the intention to choose the real option about the cloud service. For this study, five hypotheses is drawn, and a survey is conducted about the medium and small firms which are currently using cloud service to examine hypotheses. Since the study is at organizational level, 287 questionnaire replies are recalculated to 120 firms. For statistical analysis, Smart PLS and SPSS Statistics18 are used. As a result, technical risk of cloud service has significantly positive influence on security risk. Second, security risk and relational risk of cloud service has significantly positive influence on economic risk. Third, economic risk of cloud service has significantly positive influence on the intention to purchase the delay option or abandon option. Based on this result, this research discussed practical and academic implications and the limitations.

• Journal of Biomedical Engineering Research
• /
• v.24 no.4
• /
• pp.355-361
• /
• 2003

In the present study, a new Real Time Subjective Evaluation (RTSSE) system was developed. The system is composed of two parts: a sensibility input part and sensibility evaluation part. The sensibility input part receives values, which are recorded on an input board using a stylus and digital tablet, from each subject's evaluation of his/her own subjective sensibility towards a particular stimulus. The sensibility evaluation part displays the level of pleasantness and arousal on one or two dimensions in real time. An experiment was conducted in order to investigate the feasibility and effectiveness of the RTSSE system. The present study compared Galvanic Skin Response (GSR) with the RTSSE by presenting 28 subjects in their 20s with pictures arousing either positive or negative sensibility. Following the experiment, an off-line subjective assessment using a questionnaire was given to the same subjects. According to the correlation coefficients, changes in subjective sensibility caused by the positive visual stimulus were related more closely to GSR, from the positive visual stimulus, and changes in subjective sensibility caused by the negative visual stimulus were related more closely to GSR from the negative visual stimulus. The questionnaire results showed marked similarity to the average responses of the RTSSE. In conclusion, the most remarkable characteristic of the present system is that it not only assesses the average sensibility when stimuli are presented, but also shows the changing strength of sensibility over time.

• Journal of Digital Convergence
• /
• v.12 no.11
• /
• pp.99-112
• /
• 2014

This study the impact on the real earnings management credit rating (RANK), and looked at the impact on the real earnings management grade credit rating changes (decrease, increase) the effects in detail. firm for a total of 06 years for firm that are listed on the Korea Stock Exchange from 2008 to 2013 for the hypothesis - using the proceeds of the year 2,583 sample were analyzed to study. A regression analysis of the relevance of the credit rating (RANK) and real earnings measured results between the credit rating and a measure of real earnings management ACFO and ADE (+) between AMC (-) IFRS and receive relevant ADE between(+) between AMC (-) if the credit rating (RANK) is increased ACFO and is significantly sound level at 1% showed the relevance of (+) did not significantly ADE (+) 10% of AMC if the credit rating fell ACFO is (-) from AMC show the relevance of positive credit rating is dropped capital letter showed for performing real earnings management of positive even give up the future cash flow in order to reduce the cost.

• Korean Journal of Veterinary Service
• /
• v.35 no.3
• /
• pp.215-222
• /
• 2012

Species-specific $TaqMan^{(R)}$ probe-based real-time PCR assays were developed for detection of beef, pork, chicken, duck, goose and turkey. The primer and probe sets used in this study were designed to be complementary to fibroblast growth factor (FGF) for cattle and pig, mitochondrial NADH dehydrogenase (ND) subunit 3 and ND2 for chicken and duck, 12S rRNA for goose and turkey, respectively. As internal positive control we used conserved region in the ribosomal 18S RNA gene to ensure the accuracy of the detection of target DNA by real-time PCR. We confirmed that real-time PCR assays with the primer and probe sets were positive for cattle, pig and chicken intended target animal species with no cross-reactivity with other non-target animal species. Only >50 ng DNA of beef show cross-reactivity in the determination of duck. Using species-specific primer and probe sets, it was possible to detect amounts of 0.1 ng DNA of cattle and pig, 1.0 pg DNA of chicken, duck and turkey, and 0.1 pg DNA of goose for raw samples, respectively. The detection limits were 0.1 ng DNA of cattle, 1.0 ng DNA of pig and 1.0 pg DNA of chicken for DNA mixtures (beef, pork and chicken) extracted from heat-treated ($121^{\circ}C$/5 min) meat samples. In conclusion, it can be suggested that the $TaqMan^{(R)}$ probe-based assay developed in this study might be a rapid and specific method for the identification of meat species in raw or cooked meat products.

• Journal of the Korean association of regional geographers
• /
• v.15 no.1
• /
• pp.138-160
• /
• 2009

The urban housing market in Korea, especially in Seoul and the Capital region, has been revitalized with massive urban (re)developments and expanding real estate finance after the IMF crisis. This brought about a boom of housing price during the mid-2000s, which has been virtually stabilized by strong regulation policies of the previous government. But with impacts of the recent international financial crisis together with some inherent problems, the housing market of Korea faces with a worry of collapse in relation with the financial market volatility and the serious depression of real economy, and hence the current government attempts to implement strong deregulation policies on the housing market. In this paper it is argued that this kind of volatility of urban housing market seems to be caused by strategies of capital which involve continuous massive urban (re)development, residential segregation and appropriation of monopoly rent(or capital gain), and fictitious capitalization of real estates and integration of real estate market and financial market. In these reasons, the current tendency of urban housing price shows a slow downward, which seems to give the current neoliberal government a rationale for deregulation policies to prevent the downward tendency. But this paper suggests that such a slow downward of housing price shift would have positive effects on the housing market in particular and social and economic situations in general, and hence an alternative housing policy is required to realize such positive effects.

• Journal of Food Hygiene and Safety
• /
• v.34 no.3
• /
• pp.277-282
• /
• 2019

The object of this study is to compare the performance of the 3M Molecular Detection Assay 2 (3M MDA 2) and the Korea Standard Food Codex (KSFC) Method (i.e., isolation media and real-time PCR) in detecting Salmonella Typhimurium and Listeria monocytogenes in traditional Korean foods. Yuk-hwe and Yuk-sashimi (types of raw beef dishes) were artificially inoculated with $10^0-10^4CFU/25g$ of L. monocytogenes and S. Typhimurium. Citrobacter freundii and Listeria innocua were used as competitive microflora. After enrichment, the samples were analyzed using 3M MDA 2 and real-time PCR. All samples inoculated at concentrations of $10^0-10^4CFU/25g$ without competitive microflora were positive for S. Typhimurium and L. monocytogenes, as detected by 3M MDA 2 and Korea Standard Food Codex (KFSC) Method. In addition, part of the samples were positive for the presence of C. freundii and L. innocua. The 3M MDA 2 - Salmonella and Korea Standard Food Codex (KFSC) Method showed similar detection performances in Yuk-hwe and Yuk-sashimi. The 3M MDA 2 method for Salmonella and Listeria, which is a LAMP-based technology, can be used for rapid detection of S. Typhimurium and L. monocytogenes in raw beef. LAMP bioluminescence assays provide results on the subsequent day and are simple to use compared with the Korea Standard Food Codex (KFSC) Method, particularly in terms of DNA preparation.

• Proceedings of the Technology Innovation Conference
• /
• 2003.02a
• /
• pp.63-77
• /
• 2003

In this paper, we investigate the co-movements of the real GDP cycle and the telecommunications service expenditure cycle over 1980 through 2000 in Korea. We used several appropriate statistical tools to characterize and to compare the co-movements between the two aforementioned variables. The statistics presented in this paper are common, easy to Interpret and capture important visual, descriptive information and the dynamics of data that would be lost if one focused only on the unconditional correlation coefficients of de-trended data. This paper examines four possible characteristics for the co-movements of real GDP and telecommunications service expenditure in Korea. The first is that in low frequency, the cyclical movements in telecommunications service expenditure are very similar to the cyclical movements in real GDP, The second is that in the high frequency, the estimated stationary cyclical telecommunications service expenditure is not closely related to the estimated stationary cyclical real GDP. Third, according to co-spectra analysis, the strong relationships between the variables are found in the mid-term frequency and as such this empirical finding has important implications for telecommunications policy and telecommunications service implementation strategies. Finally, in the short-term period, our empirical results support telecommunications network theories such as path dependence properties, lock-in, positive feedback externality, and network externality.