• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.558-562
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• 2010

SDS-PAGE of extracted surface-associated proteins of Lactobacillus rhamnosus strains E/N, Oxy, and Pen, was performed. The obtained protein patterns allowed differentiation of the examined strains, which was not accomplished by the commonly used RAPD genotypic method. The differentiation by the SDS-PAGE method proved to be a useful tool for strain-specific identification, which was further confirmed by 2DE analysis. Therefore, it can be used as an alternative or complementary method for both conventional and genotypic identification procedures, especially when closely related lactobacilli isolates are identified.

• Journal of Biosystems Engineering
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• v.30 no.4 s.111
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• pp.249-253
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• 2005

This study was carried out to develop enzyme biosensor for lactic acid bacteria. Lactic acids produced by lactic acid bacteria (LAB) was measured and good correlation $R^2=0.98$ between LAB count and lactic acids concentration was found. Hydrogen ion produced by L-lactate dehydrogenase (L-LDH) was measured by a potentiometer. Glutamic-pyruvic transminase (GPT) was used for eliminating inhibitor in the reaction. Polyacrylamide gel was used for immobilizing matrix of the sensor. The biosensor was tested and showed good feasibility with $R^2=0.99$ on validation.

• Journal of Microbiology and Biotechnology
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• v.2 no.3
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• pp.161-165
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• 1992

The gene encoding the bacteriolytic enzyme cell wall peptidoglycan hydrolase from alkalophilic Bacillus sp. was cloned in E. coli using pBR322 as a vector. A recombinant plasmid, designated pYTR451, was isolated and the size of the cloned HindIII fragment was found to be 4.8 Kb. The cell wall hydrolysis activity of an extract of the E. coli harboring the recombinant plasmid pYTR 451 was detected by SDS- polyacrylamide gel containing 0.2% (w/v) purified cell wall of Bacillus sp. The molecular weight of the enzyme was estimated to be about 27, 000 corresponding to the molecular weight of the Bacillus sp. bacteriolytic enzyme. The recombinant plasmid was found to contain the fragment originated from Bacillus sp. YJ-451 chromosomal DNA by Southern hybridization.

• Microbiology and Biotechnology Letters
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• v.24 no.2
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• pp.173-177
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• 1996

Bacillus thuringiensis subsp. morrisoni PG-14 is a gram-positive soil bacterium producing mosquitocidal parasporal inclusions composed of several crystal proteins. Among these crystal protein genes, cryIVD gene is one of major component which has 72 kDa in size. However, these parasporal inclusions sink quickly from the surface of water where mosquito larval feeding occurred. To develope mosquitocidal cyanobacterium, therefore, we constructed the expression vector, pCYASK 5-1 harboring cryIVD gene. The expression vector, pCYASK5-1 was transformed into the cyanobacterium Syne- chocystis PCC6803 reported as a natural mosquito larval food source and the transformants were selected with kanamycin. Expression of IVD gene in transformant was characterized by SDS-polyacrylamide gel electrophoresis (PAGE) and immunoblot analysis. The mosquitocidal activity of a transformant was determined with Culex tritaeniorhynchus. The results showed that, the transformed cyanobacterium is toxic to mosquito larvae and will be expected as a potential agent that is used for mosquito control.

• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.402-407
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• 1987

Glucose oxidase from Aspergillus niger KUF-04 was purified homogeneously by the procedure of seven steps including crystallization. The ball-like crystalline enzyme was obtained from the 23-fold purified enzyme solution. The glucose oxidase was found to be composed of two identical subunits and the molecular weight of the enzyme and its subunit were estimated to be about 210, 000 and 110, 000 by HPLC and SDS-acrylamide gel electrophoresis, respectively.

• Microbiology and Biotechnology Letters
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• v.15 no.6
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• pp.420-424
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• 1987

Isocitrate lyase from crude extract of Saccharomycopsis lipolytica ATCC44601 and MX9-11RX8 temperature-sensitive mutant was purified about 54 times and 87 times, respectively by ammonium sulfate fractionation, Toyo peal HW-55F gel filtration and DEAE-Cellulose ion exchange chromatography, The molecular weight of the purified isocitrate lyase from this yeast was estimated to be 230, 000 by gel filtration on Sephadex G-200, and SDS-polyacrylamide Eel electrophoresis showed that the enzyme consisted of four identical or similar subunits with a molecular weight of 59, 000 and the enzyme showed optimum activity at pH 6.9.

• Microbiology and Biotechnology Letters
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• v.22 no.6
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• pp.627-635
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• 1994

The $\beta$-xylosidase from Penicillium sp. FX-102 was purified by 40~80% ammonium sulfate saturation, CM-Cellulose column chromatography, Sephadex G-200 gel filtration, and isoelec- tric focusing. The optimum pH and temperature for the activity of the $\beta$-xylosidase was pH 4.5 and 50$\circ$C, respectively. The enzyme was stable at the pH range of 4.5~5.5, and at 55$\circ$C for 10 min. The molecular weight of the enzyme was estimated to be about 300,000 daltons by Sephadex G-200 gel filtration and 310,000 daltons of monomer by SDS polyacrylamide gel electrophoresis. Isoelectric point of the enzyme was determined to be pH 4.4. The enzyme activity was strongly inhibited by Hg$^{2+}$, Ag$^{2+}$, n-bromosuccinimide and p-chloromercuribenzoate. Xylobiose (10 mM) was completely decomposed to xylose after 8 hrs enzyme reaction with 2 units of the $\beta$-xylosidase.

• Journal of Veterinary Clinics
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• v.15 no.2
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• pp.274-278
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• 1998

The biological father of two Golden Retriever puppies was determined between two proposed stud dogs by using microsatellite DNA analysis. DNA was obtained from all the relevant dogs by buccal swabbing and three loci of tetranucleotide repeat microsatellite were PCR-amplifiedl and analyzed by polyacrylamide gel electrophoresis and silver staining. One of the two proposed stud dogs was assigned as the biological father of the puppies by the genotyping. The result demonstrated that the microsatellite DNA analysis is a simple, efficient method of paternity test in dogs.

• Proceedings of the Korea Technical Association of the Pulp and Paper Industry Conference
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• 2006.06b
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• pp.251-256
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• 2006

The purpose of this study was to confirm multiple retention system of C-PAM, A-PAM and Inorganic micro particles vs. traditional micro particle system and dual polymer system by measuring retention, drainage and formation using RDA HSF and Techpap 2D -F Sensor The benefits of dual polymer system were easy to use, low chemical consumption and good retention property but defect was worse drainage property than inorganic microparticle systems. On the other hand, Inorganic microparticle system had benefit of good drainage effect but defects were difficult to use, high chemical consumption. Therefore, we tried to find optimal morphology of polyacrylamide and applied to multiple retention system of C-PAM, A-PAM and inorganic microparticles to compensate defects of both of retention systems. As a result, we found the performance of branched C-PAM, branched A-PAM and inorganic micro particle triple system was more appropriate than traditional inorganic mircoparticle systems or dual polymer systems by comparing retention, drainage and formation.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.32 no.1
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• pp.26-32
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• 2000

The impact of major surface chemical properties of silica sols on the retention and drainage performance of the silica based micrparticle system, Compozil was investigated using four different silica sols. And the effect of silica properties on the interactions with cationic starch and cationic plyacrylamide has also been identified. The surface charge density and the stability over pH of silica sols were increased by introducing aluminosilicate anions at surface. It was found that the charge density of silica sols determined the addition level necessary to attain the maximum retention and drainage. When silica sols were combined with cationic starch, the change density of the product was the critical properties and the degree of microagregation was of minor importance. In the cationic polyacrylamide system the degree of colloid structure appeared to be a more critcial property than the charge density of silica sols.