Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Paternity test in dogs by DNA analysis

유전자감식에 의한 개에서의 친자감별

  • Lee, Hang (College of Veterinary Medicine, Seoul National University) ;
  • Chae, Young-Jin (College of Veterinary Medicine, Seoul National University) ;
  • Lee, Byeong-Chun (College of Veterinary Medicine, Seoul National University)
  • 이항 (서울대학교 수의과대학) ;
  • 채영진 (서울대학교 수의과대학) ;
  • 이병천 (서울대학교 수의과대학)
  • Published : 1998.06.01
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Abstract

The biological father of two Golden Retriever puppies was determined between two proposed stud dogs by using microsatellite DNA analysis. DNA was obtained from all the relevant dogs by buccal swabbing and three loci of tetranucleotide repeat microsatellite were PCR-amplifiedl and analyzed by polyacrylamide gel electrophoresis and silver staining. One of the two proposed stud dogs was assigned as the biological father of the puppies by the genotyping. The result demonstrated that the microsatellite DNA analysis is a simple, efficient method of paternity test in dogs.

Keywords

References

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