• Korean Journal of Environmental Agriculture
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• v.21 no.4
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• pp.261-268
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• 2002

Biodegradation of monochlorophenols by wood rot fungi such as Daldina concentrica, Trametes versicolor and Pleurotus ostreatus was evaluated by determining their resistance or toxic test and biodegradability. The metabolites of monochlorophenols were also analyzed. Among the three fungi, T. versicolor was the most resistant to 200 ppm of 2-, 3- and 4-chlorophenols, and did not show any inhibitory mycellium growth. But D. concentrica had a little inhibition effect at more than 100 ppm of 3- or 4-chlorophenol. Control cultures of P. ostreatus took even 14 days far the completion of mycellium growth, but the hyphal growth was improved when 2- or 3-chlorophenol were added to the culture. In biodegradation analysis, P. ostreatus showed the highest degradation of 2- or 3-chlorophenol, while T. versicolor was the most effective in 4-chlorophenol. D. concentrica and P. ostreatus slowly degraded 4-chlorophenol. However, T. versicolor had similar degradation capability in the three monochlorophenols, suggesting that the biode- gradation nude is dependent on the fungi as well as the type of monochlorophenol. Several metabolites such as 1,3,5-trihydroxyl benzene, 1-ethyl-1-hydroxyl pentane, 2-propenoicacid, methylmalonic acid and 2-methyl-4-keto-pentan-2-ol were found as products of primary oxidation of 2-, 3- and 4-chlorophenols by intact fungal cultures. fatty acids including tetradecanoic, heptadecanoic and octadecanoic acids were also detected The order of increase of mycellium weight during incubation were P. ostreatus > T. versicolor > D. concentrica. The pH in the culture was not constantly changed depending on incubation days, but the mycellium weight was slightly increased, indicating that the biodegradation of monochlorophenol might have low relationship with the mycellium growth Laccase activities of T. versicolor and P. ostreatus were continuously increased depending on the incubation days, suggesting that the ligninolytic enzyme activity play an important role in the biodegradation of monochlorophenol.

• Journal of Mushroom
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• v.3 no.1
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• pp.35-39
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• 2005

Highly Activated Calcium(below HAC) is the oxidized calcium made by dissolving shell materials with high voltage about 15,000V and high temperature ($1,500{\sim}5,000^{\circ}C$). This HAC is a material with a very high degree of purity without toxicant. This HAC decreases chemical reaction so the degree of being active and dissolving living material is outstanding. The effects of HAC on the propagation of mushrooms are following. In the case of the Pleurotus ostreatus, when controlling media moisture by mixing with the HAC, mycelium cultivating days were shortened by 2days. The day required for primordial formation after inoculation(DPI) were one day faster. The number of stem was 15 and individual weight was 248g/850cc, a 6.5% increase. In the case of Pleurotus eryngii, when controlling media moisture by mixing with the highly activated calcium, mycelium cultivating days were shortened by 3days. DPI were l day faster. The day required for colonization after inoculation was shortened by 6days and individual weight was 108.8g/850cc, a 9.7% increase. In the case of Flammulina velutipes, the highly activated calcium was the best for scraping up mycelium. Mycelium incubating days were shortened by 2days. DPI were shortened by 3days. The day required for colonization after inoculation was 1day faster and the period of cultivation was shortened by 3days. Individual weight was 165g/850cc, a 6.7% increase. In the case of Lentinula edodes, when mixing media with the highly cultivated calcium, cultivating days were shortened by 3 days. The days for becoming brown in color were 2 days faster and the days of the first harvest were shortened by 4 days. The weight of mushrooms was 169g/2kg, a 9.7% increase.

• Journal of Practical Agriculture & Fisheries Research
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• v.3 no.1
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• pp.70-78
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• 2001

The objective of this study was to determine effects of corn germ meal(CGM) and condensed molasses fermentation solubles(CMS) as the replacement of rice bran on mycelial growth and density in various mushrooms. The results of this study showed that CGM and CMS might be utilized more efficiently than rice bran in culturing mushrooms. The results are as follows; 1. Mycelial growth of Pleurotus ostreatus was remarkably higher than that of control when the concentration of CGM and CMS was 10%, respectively. However, the mycelial density was lower than that of control. Thus, Considering the mycelial growth as well as density, it was found that 20% supplements of CGM and CMS resulted in the best with 78mm/10d and 71mm/10d, respectively. 2. Mycelial growth of Pleurotus eryngii in the treatment group of 10% CMS and 20% CGM showed 74mm/10d and 67mm/10d, respectively. This result tended to be equal or somewhat lower compared with that of control(74mm/10d). 3. Mycelial growth of Flamulina velutipes was 87mm/10d in the treatment of 10% CMS, 79mm/10d in the treatment of 20% CGM, which showed significantly higher than that of control(56mm/10d). When the contents of CGM and CMS were mixed with the amount of 10-30%, Thus, it was assumed that the quantity of mushrooms may increase with the supplements of CEM and CMS. 4. Mycelial growth of Lentinus edodes in the treatment group of 10-40% CMS and 10-30% CGM showed 58-52mm/10d and 56-49mm/10d, respectively. This result was significantly higher that control(47mm/10d). 5. When CMS and CGM were supplememted with the level of 10-30%, mycelial growth rate of Ganoderma lucidum showed 76-72mm/10d and 74-69mm/10d, respectively, which are higher than those of control.

• Journal of Microbiology and Biotechnology
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• v.8 no.2
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• pp.129-133
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• 1998

The white-rot fungi Phanerochaete chrysosporium ATCC 24725, Pleurotus ostreatus ATCC 32783, Lentinus edodes ATCC 24462, and Trametes versicolor ATCC 42530 were studied for their ability to degrade lignin, phenanthrene, and anthracene. Lignin in rice-straw was degraded by 14.4, 28.73, and 33.88% by P. chrysosporium, T. versicolor, and P. ostreatus, respectively. Approximately 12% and 83% of phenanthrene was degraded in 1 and 5 days, respectively, when the pre-grown mycelIium matrix of P. ostreatus. was incubated with 10 ppm of phenanthrene in modified Kirk's medium (nitrogen limited) at $25^{\circ}C$. Approximately 2%> and 61% of phenanthrene was degraded when the phenanthrene concentration was increased to 30 ppm. Similar trends were observed with phenanthrene using P. chrysosporium. Mycelial growth of T. versicolor was less inhibited at 30 ppm phenanthrene than for P. ostreatus and P. chrysosporium. Better degradation of phenanthrene by T. versicolor may be attributed to better mycelium growth. One hundred percent of 15 ppm anthracene was degraded in 10 days by both P. chrysosporium and T. versicolor. 40 ppm anthracene inhibited the mycelial growth of P. chrysosporium. lignin peroxidase activity, which was previously reported to be involved in initial phenanthrene oxidation, was also detected from the culture broth of the strains tested. The rates of lignin peroxidase production in the cultures were not consistent with the rate of PAH hydrolysis during incubation.

• Journal of Life Science
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• v.16 no.2 s.75
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• pp.225-230
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• 2006

To elucidate the effect of temperature on the fruit body growth and structure of pleurotus ostreatus cultivar Chunchu No.2, microstructures were observed with scanning- and transmission electron microscope. Pileocystidia were well developed on the surface of pileus in the fruit body cultivated at $7^{\circ}C$. As the increasing temperature for fruit body development, thickness of pileus, thickness of stipes and length of stipes shown thin, thick and long, respectively. Color of pileus was also changes to whitish grown under high temperature. Physical structure shown as hardness and gumminess of stipes grown at high temperature, fruit body were soften than that of low temperature. Microstructures of fruit body grown at high temperature shown fast-discharge of basidiospore and sytoms aging. vacuolation in the cell observed at mycelium grown under high temperature. Optimum temperature for vegetative growth of fruit bodies were $15^{\circ}C$, and shown regular arrangement of mycelium on the surface of stipes.

• Journal of Mushroom
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• v.2 no.4
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• pp.218-221
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• 2004

Jinmi oyster mushroom(Pleurotus ostreatus) were bred in Mushroom Research Institute, Gyonggi Province A.R.E.S in 2003. This oyster mushroom were bred and cultivated one after mating single spores collected from K3-2 and ASI2018-249. The major characteristics of the mushroom are showing a lot of pinheadings, the gray-colored and infundibuliform pileus. The optimum temperature for the mycelial growth was around $25{\sim}30^{\circ}C$ and that for the pinheading and growth of fruitbody was around $15{\pm}1^{\circ}C$. Around 22 days at $20^{\circ}C$ were required for incubation of Jinmi and the yield was shown high by 145.9g/bottle.

• The Korean Journal of Mycology
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• v.27 no.6 s.93
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• pp.420-423
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• 1999

A Sciarid fly, Lycoriella mali was a serious pest in commercial production of the cultivated mushroom, Pleurotus ostreatus. It was found in light trap and compost samples taken from mushroom houses during all growing seasons. The fly population 'was increased with the development of mushroom growth, the larvae occurred about 30 days after spawn inoculation in compost. The highest population was developed spring cultivation season (March to June) and decreased during summer season (August to September). The larvae feed on mycellium and compost; injury to the growing mushroom mycellium and the subsequent consumption of the primordia; they entered and tunneld sporospore stem and infect pinhead formation which would be resulted in decrease of mushroom yield.

• The Korean Journal of Mycology
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• v.36 no.1
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• pp.9-15
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• 2008

For the detection of contaminated liquid spawn, we selected suitable medium, indicator and developed method of diagnosis. The growth of pathogenic bacteria, Pseudomonas sp., and fungi, Trichoderma sp., in YPL media was better than in PDA and NA. In addition, the changes of color and absorbance of media were obviously showed when contaminated liquid spawn by pathogenic bacteria and fungi was incubated on YPL including phenol red for 48 hour at $25^{\circ}C$. The color of YPLP after incubating of infected liquid spawn by Pseudomonas sp. and Trichoderma sp. were changed from orange to red and to scarlet, respectively. Whereas, the color of YPLP after incubation of only Pleurotus ostreatus indicated yellow at liquid spawn. Therefore, it is possible to easily distinguish contaminated liquid spawn by color of change in YPLP.

• Journal of Mushroom
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• v.4 no.3
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• pp.101-105
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• 2006

This was performed to improve the working conditions of oyster mushroom cultivation on bed using rice straw of which binding and cutting procedure need a lot of time. There was a little difference in physiochemical components between rice straw and cotton waste C/N ration of cotten waste was, however, similar to rice straw as 84-85. If is feasible to use as substrate material. The average yield of a net was 1450g in six frustration harvest, when the size of an onion net was $35{\times}30cm$, mixture ratio of rice straw to cotton waste was 40:60(V/V). Cultivation method using an onion net saved 3 days for preparation, 4 hours for inoculation compared to normal bed cultivation. This method was analysed to give 5% economical benefit.

• Current Research on Agriculture and Life Sciences
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• v.30 no.1
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• pp.51-59
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• 2012

Fresh oyster mushrooms (Pleurotus ostreatus) were gamma-irradiated at 0, 1, 2, and 3 kGy. The effects on various quality attributes were determined during storage at $5{\pm}1^{\circ}C$. Color changes were more prominent in the cap region than the stem part. At the start of storage increase of Hunter's L-value (lightness) was observed in the caps of 2 and 3 kGy-irradiated samples. The L-value was higher in the all irradiated samples during storage. The trend was different in the case of stem region, where L-value decreased upon irradiation, but remained high throughout storage. The ${\alpha}$-value declined, whereas the b-value increased following irradiation. Irradiation showed a dose-dependent effect on the firmness, which was clearer during storage, but the samples irradiated at 1 kGy maintained an overall better texture than other irradiated samples. The weight loss was also higher in the all irradiated samples during storage. The samples irradiated at 1 kGy showed good physical appearance without any fungal attack at the end of storage; however color change in cap region was quite apparent. The ultra-structural drastic effect of irradiation was understandable using scanning electron microscopy. E-nose analysis demonstrated a clear change in the volatile profiles of all irradiated samples. Although the effect of irradiation on quality characteristics was quite clear but the all irradiated samples were free from fungal attack that was observed in the case of control sample.