• 생명과학회지
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• 제15권5호
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• pp.739-742
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• 2005

본 연구는 배지에 첨가된 목초액 및 코코넛액이 석곡 유묘의 증식 및 기내 개화에 미치는 영향을 조사하기 위하여 실시되었다. 목초액이 석곡 유묘의 증식에 미치는 영향은 NAA 0.1 mg/L와 kinetin 1.0 mg/L 가 첨가된 $H_{3} P_{4}$ 배지에 목초액 1.0 ml/L를 처리했을 때 유묘의 증식 및 생장이 양호 하였다. 코코넛액은 30 ml/L가 첨가되었을 때 유묘의 증식 및 생장이 가장 양호하였다. 기내 개화 역시 NAA 0.1 mg/L 와 kinetin 1.0 mg/L 가 첨가된 $H_{3} P_{4}$ 배지에 목초액 1.0 ml/L 또는 코코넛액 30 ml/L가 첨가되었을 때 전체 개체의 $ 20\% $에서 기내개화가 일어났다

• 한국작물학회지
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• 제48권6호
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• pp.438-441
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• 2003

울금의 기내대량번식을 위한 경정배양에 알맞은 기본배지의 종류와 기내증식 및 생장에 미치는 식물생장조절제의 농도를 구명하고자 본 실험을 수행한 결과는 다음과 같다. 울금의 경정은 MS(Murashige and Skoog)기본배지에 치상한 경우 Wanspojen, LS, White 배지보다 shoot와 root의 형성율이 높은 경향이었다. 기내의 유식물체 생장은 오옥신(NAA 0.5-l.0 $\textrm{mg}/\textrm{l}$)과 사이토키닌(BA 1.0-5.0 $\textrm{mg}/\textrm{l}$) 혼용처리가 효과적이었다.

• 한국약용작물학회지
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• 제1권1호
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• pp.38-42
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• 1993

양하(襄荷)를 대양번식(大量繁植) 하고자 경정부위(莖頂部位)의 기내배양(器內培養) 방법(方法)을 확립(確立)하기위하여, 경정부위(莖頂部位)의 생장(生長)에 알맞은 기본부지(基本部地) 및 식물생장조절제(植物生長調節劑)의 농도(濃度)를 구명(究明)하고자 본(本) 실험(實驗)을 수행(遂行)하였던 바 그 결과(結果)를 요약(要約)하면 다음과 같다. 1. 양하(襄荷)의 경정부위(莖頂部位)는 치상후(置床後) $10{\sim}12주(週)$ 후(後)에 기관(器官) 분화현상(分化現像)이 나타나서 shoot와 root가 발생(發生)되었다 2. 양하(襄荷)의 경정(莖頂)은 MS기본부지(基本部地)에 치상(置床)한 환경(環境) 비교적(比較的) 생장(生長)이 잘 되었다. 3. 기내(器內)의 Plantlet의 생장(生長)은 식물생장조절제(植物生長調節劑) NAA 0.5ppm과 BA 5.0ppm의 혼합(混合) 첨가배지(添加培地)에서 좋은 경향(傾向)이었다.

• Journal of Plant Biotechnology
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• 제2권3호
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• pp.123-128
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• 2000

To enhance in vitro plantlet regeneration efficiency of soybean through embryogenesis, the culture conditions such as material part and size of immature seed, 2,4-D, pH and solidifying agents for somatic embryogenesis were investigated. Somatic embryogenesis was induced from the immature embryo, immature cotyledon and embryonic axis explants of the immature seed on MS medium supplemented with 2.0 mg/L 2,4-D. The highest rate (up to 22.9%) of somatic embryogenesis was obtained from the immature cotyledon, following embryonic axis and the immature embryo. The rate varied with the developmental stages of seed. The maximum rate (25.4%) of embryogenesis was obtained from 3-4 mm length of the seed (after 25 days of flowering). The optimum concentration of 2,4-D for embryogenesis was 10 mg/L. The optimum pH was at 5.8 and solidifying agent for medium was better with 0.4% gelrite than with agar. For rapid multiplication of shoot tips from the germinating somatic embryos, they were cultured on MS medium containing 2 mg/L indole-3-butyyic acid (IBA) and 1 mg/L 6-benzyladenine (BA). After then somatic embryos with one and three cotyledons were transferred to the growth regulator free medium. The medium exhibited the higher rate (ca. 50%) of development than the multiplication medium.

• Plant Biotechnology Reports
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• 제3권3호
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• pp.259-266
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• 2009

A plantlet regeneration protocol was developed on pot-grown mature plants of Elaeocarpus robustus Roxb. cv. Dwarf from nodal and leaf explants. The best yield of adventitious shoots was achieved from the leaf-derived calli in a modified MS ($MMS_1$, half strength of major salts, full strength of minor salts, and vitamins) medium containing $4.0{\mu}M$ BA + $4.0{\mu}M$ Kn + $0.5{\mu}M$ NAA + 15% coconut water (CW). The shoot multiplication rate was amplified about twofold per culture after the addition of 15% CW to the medium. The rate of shoot multiplication reached maximum at the 5th subculture, and it maintained this rate throughout the 3 subsequent subcultures. The best rooting in vitro was investigated by subculturing the microcuttings in an $MMS_2$ (half strength of both major salts and minor salts and full strength of vitamins) medium containing $1.0{\mu}M$ IBA in the dark for one initial week at $30^{\circ}C$, followed by subculturing them in a plant-growth regulator (PGR)-free medium in the light. The plantlets raised in vitro were successfully established under ex vitro conditions.

• 한국산림과학회지
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• 제95권2호
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• pp.155-159
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• 2006

An efficient method for in vitro propagation of the medicinal plant Hovenia duleis, was established. Plantlets for micropropagation of H. dulcis were obtained from in vitro germinated seeds. The effectiveness of various levels of cytokinins (BAP, Kinetin and TDZ) on multiple shoot formation from stem nodes was tested. BAP (1.0 mg/L) treatment induced highest number of multiple shoots. The growth pattern of plantlet on various culture media was undertaken. The shoot elongation was optimal on 2MS basal medium without growth regulators. The in vitro rooting ability of H. dulcis shoots was examined with two-auxins IAA and IBA. The IAA (1.0 mg/L) treatments induced earliest rooting with maximum number of roots and root growth. Rooted shoots were transferred directly to small pots with artificial soil and such established plant exhibited a normal growth pattern similar to wild plantlet.

• Journal of Plant Biotechnology
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• 제6권3호
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• pp.199-204
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• 2004

The medicinal value of the genus Cleome justifies bio-technological studies of Cleome rosea, a Brazilian annual species from sandy coastal ecosystems (restinga), which have been submitted to an intense process of antropogenic degradation. In the present work, was analyzed the influence of cytokinins, 6-benzyladenine (BA) and 6-furfurylaminopurine (kinetin) added to the Murashige and Skoog medium (MS), on the proliferation capacity of explants from the stem axis (hypocotyl, node and internode) for a period of five monthly subcultures (150 days). Regardless of the explant sources, plantlet regeneration by direct and indirect organogenesis was observed. The largest number of shoots proliferated through direct organogenesis was obtained on medium with 4.4 $\mu{M}$ BA. Also, the highest proliferation capacity through indirect organogenesis was found on medium with 4.4 $\mu{M}$ BA + 4.6 $\mu{M}$ kinetin. The presence of kinetin alone was not effective for multiplication of the species. Elongation and rooting were obtained when shoots were transferred onto growth regulator-free medium, and acclimatization rates from 70% to 81% were achieved depending on explant sources used. Plants were then successfully established in soil and showed normal phenotypes.

• Journal of Plant Biotechnology
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• 제8권1호
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• pp.37-41
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• 2006

In vitro mass multiplication of Vanilla planifolia was investigated using node as explant. Multiple shoots were developed in MS medium supplemented with $2.0mgl^{-1}$ 6-benzylaminopurine and $1.0mgl^{-1}$ $\alpha$-naphthalene acetic acid. Multiple shoots were maintained for 6-T weeks with regular subculturing at the end of $3^{rd}$ week onto fresh medium. The maximum number of shoots at the rate of 12.8 per node segment was achieved over a period of four weeks. The elongated shoots were separated from the shoot clusters and were transferred onto half strength MS medium supplemented with indole-3-acetic acid ($1.0mgl^{-1}$) over a period of 28 days for induction of roots. The development of roots was observed on $7^{th}$ day of incubation. The in vitro raised plantlets were transferred to poly-cups, covered with polyethylene sheets and maintained under shade net for 25 days for hardening. Finally these plants were transferred to field and recorded that 85 % of tissue cultured plants were survived. From the present study, a simple and efficient micropropagation protocol was developed for Vanilla planifolia using single node segments as explants.

• 한국작물학회지
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• 제46권6호
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• pp.464-467
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• 2001

황칠나무의 선발된 우량개체군의 집단재배시 우량해체간의 교잡에 의해서 형성된 종자를 증식하기 위한 기내배양체계를 확립하기 위해서 기내 발아, 증식, 발근에 적합한 식물생장조절제를 구명하기 위하여 본 실험을 실시한 결과는 다음과 같다. 황칠나무 종자의 무균발아에 적합한 배지로는 MS(Murasinge Skook)기본배지가 발아율이 높고 유식물체(plantlet)의 생장이 양호하여 알맞는 배지로 판단 된다. 기내에서 신초의 증식과 생육에 효과적인 식물생장조절제는 사이토키닌(BAP 0.1~l.0 mg/l)과 오옥신(NAA 0.5~1.0 mg/l)의 혼용처리가 효과적이었다. 또한 기내의 발근은 오옥신의 첨가에서 쉽게 유도되었으며, 그 중에서 NAA1.0 mg/l첨가에서 가장 양호하였다.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.1045-1054
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• 1996

Tissue culture, or micropropagation , is being used for the vegetative multiplication of several hundred millions of superior plants annually for horticulture and forestry. It is often more expensive than other forms of propagation using cuttings or seeds, because it is labor intensive and more specialized . The aim of automation is to reduce the cost per plantlet by reducing labor input, and finally, to yield profit, as business activity . Labor usually account for 70-80% of th ein vitro and ex vitro cost. This paper aspects of tissue culture automization , such as technical and economical approaches in view of automization.