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First Report of Pink Rot of Potato (Solanum tuberosum) Caused by Phytophthora erythroseptica in Korea (Phytophthora erythroseptica에 의한 감자 홍색부패병 발생)

  • Ryu, Kyoung-Yul;Kim, Jeom-Soon;Kim, Jong-Tae;Hahm, Young-Il
    • Research in Plant Disease
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    • v.9 no.1
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    • pp.32-35
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    • 2003
  • Pink rot of potato (Solanum tuberosum L.) occurred at Pyeongchang in Gangwon and at Bosung in Junnam province since 1999. The disease incidence in the surveyed areas was about 5% of harvested potatoes in 2002. Affected tubers showed a dull brown appearance and the lenticels and eyes on tubers turned dark brown. The cut surface of the diseased tuber colored faint pink and the entire surface of the diseased tuber becomes deep salmon pink within 30 min. The pathogen isolated from the diseased tubers was identified as Phytophthora erythroseptica based on morphological and cultural characteristics. Mycelial mat was fairly fluffy, rosette or stellate patterns and rounded or angular hyphal swellings were farmed in water, Temperature for mycelial growth was ranged from 5 to 3$0^{\circ}C$ and optimal temperature was $25^{\circ}C$. Non-papillate sporangia were persistent on stalk and ellipsoid, ovoid, obpyriform or distorted in shape, often with a constriction distal in the middle. Size of sporangia was 41.3~69.6$\times$26.8~47.4 (av, 55.5$\times$37.1) ${\mu}{\textrm}{m}$. Sexuality of Phytophthora erythroseptica was homothallic. Oogonia were 30~46 ${\mu}{\textrm}{m}$ in diameter and oospores were 28~35 ${\mu}{\textrm}{m}$ in diameter, Elongated or cyclindrical antheridia were all amphigynous. This is the first report on potato pink rot caused by Phytophthora erythroseptica in Korea.

Studies on the Method of Ground Vegetation Establishment of Denuded Forest Land in the Mudstone Region - The Characteristics of Mudstone and Speeded-up Reforestation - (니암지대황폐림지(泥岩地帶荒廢林地)의 지피식생(地被植生) 조성방법(造成方法)에 관(關)한 연구(硏究) - 니암특성((泥岩特性)과 조기녹화(早期綠化) -)

  • Chung, In Koo
    • Journal of Korean Society of Forest Science
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    • v.19 no.1
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    • pp.1-23
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    • 1973
  • The results of ground vegetation experiment conducted at completely denuded forestland in the mudstone region are summerized as follows: On the reaults of soiling quantity the effect of soiling was observed where depth of soiling over 10 cm was practiced, and a plot where treated with 15cm soiling and without fertilizer showed poor growth and it was even worser than the plot where soiling was practiced only 1 cm in thikness but applied adequate amount of fertilizers. The depth of slits between 30cm and 40cm showed no significant difference in the effect. A plot where covered with vegetation bag showed somewhat better results in seed loss and early growth but no differences observed in the fall result over the none covered plot. And then, it is recommendable to have soiling over 10cm in thikness with slit of 30cm and 30cm in depth and to apply 30 gram of fertilizer (22;22:11, 50 gram) per slit. On various soiling materials trial there were no striking differences in the effect of soiling between weathered granite soil, wheathered tuffs soil and weathered mudstone soil. In the treatment with various green materials, a plot treated with straw mat showed a significant difference at 1 percent. The results show that weathered mudstone soil is effective to use as soiling materials and straw mat treatment was better. On forest fertilization trial, in the mudstone region where red and black pine trees already existing at a rate of 2,000-3,000 trees per hectare had applied 110kg of compound fertilizers (9:12:3 and 22:22:11) per hectare basis in terms of plant nutrient. As a result, the difference in effect between the compound fertilizers was not found however the leaf color and leaf length of the fertilizer added plot showed darker and longer at 30 percent over the no fertilizer received plot. Compound fertilizers, 14:37:12 and 9:12:3 were applied to alder trees at a rate of 20 gram and 40 gram per tree in terms of plant nutrient and a remarkable growth accelerantion was observed where 40 grams of plant nutrient applied. The effect difference between the compound fertilizers was not found. On investigation of tree root elongation, forty years old red pine trees showed only 15cm tap root elongation through mudstone while black pine had 23 cm tap root elongation. The total length of supporting root elongtion of red and black pines showed 20 and 13 meters, respectively. The tap roots of Black locusts were not able to elongate through mudstone, however, the supporting roots tended to develop to the underneath of pine tree where some moisture content is available. Black locusts And grown on the residual soil of mudstone normally die between 8 to 10 years. The red pine trees show flat in tree shape while black pine had triangle in the shape. With the results it can be said that in an artificial reforestation in denuded forest land of the mudstone region the adequate slit and enough amount of fertiliizer application must be provided for the succesful performance of the program. On integrated experimental results of 1972. for the establishment of ground vegetation on the completely denuded forest land in mudstone region, soiling could be effectively practiced with weathered mudstone soil and it would not specially necessiate to have either weathered granite or tuffssoil for the soiling. And the soiling depth should be more than 10 cm in thickness. Among green materials used the straw mat proved to be the most effective reatment. Three major factors which enable to establish ground vegetation by the shortest period of time: A. Physical improvement of soil is necessary to breakdown of the horizontal cracks sushas Slit, contour line plot, seeding hole and etc., and soiling with weathered mudstone soil. B. Chemical improvement of soil: is needed sufficient amount of fertilizer application 300~400kg ha, $N+P_2O_5+K_2O$), and increased production of ground covering and expedite resolution of the vegetation (ground vegetation, fallen leaves and twigs). C. Complete establishment of the basic structure for the erosion control (Prevention of surface soil erosion)

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Considerations of Countermeasure Tasks in the Fields of Forest and Forestry in Korea through Case Study on "The Nagoya Protocol (Access to Genetic Resources and Benefit Sharing)" ("유전자원의 접근과 이익공유(ABS)" 사례연구를 통한 국내 산림·임업분야 대응과제 고찰)

  • Lee, Gwan Gyu;Kim, Jun Soon;Jung, Haw young
    • Journal of Korean Society of Forest Science
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    • v.100 no.3
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    • pp.522-534
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    • 2011
  • The aim of this study is to draw forth the tasks for establishing the right of native biology in Korea through the case study on 'Access on genetic resources and Benefit Sharing'. For this purpose, this study decided on its research subject by selecting Hoodia, on which ABS treaty was made the most recently, through the examination of the representative ABS precedents on plant species. This study analyzed the process background of ABS on Hoodia, and compared & analyzed the ABS procedures of 'Bonn Guidelines' adopted by the 6th Conference of the Parties of the Convention on Biological Diversity in 2002 and Hoodia case. Together with the ABS major issues in common drawn as a result of this analysis, and "Nagoya Protocol" adopted by the 10th Conference of the Parties of the Convention on Biological Diversity, this study intended to shed a light on the impending tasks which Korea faces at present and its role relationship. The research results are as follows: 1. It is required that species habitats should be divided based on biological classification and its subsequent community should be established with the development of infrastructure such as a community's independent production, management and monitoring of bio-species. 2. There needs to be a designation of ABS National Focal Point for sharing of ABS-related general information, boosting of implementation of the relevant convention. 3. There needs to be the establishment of ABS convention system consequent on legislative, administrative, political procedures, and designation of the Competent National Authorities for the provision of the format of Prior Informed Consent (PIC) and Mutually Agreed Terms (MAT) and their contents assessment and confirmation. 4. There should be the establishment of integrated management system of ABS-related research and development of forest biological resources and its relevant research projects. 5. There should be information development through the distribution of responsibility and role between the ministries and offices concerned according to bio-resources, and there needs to be efforts in aiming for opening a working group of academic-industrial institutions for developing a mutually interchangeable system. 6. It's required that the efficient access between industrial circles and the people should be promoted by setting up ABS support center of biological resources in ministry and office's charge. 7. There should be a selection of a national supervisory organization for securement of the right of a local community and monitoring of ABS convention implementation, and a countermeasure system for preventing outflow of forest bioresources. Conclusively, it's judged that it will be possible to inquire into the countermeasures for the establishment of the native forest biology dominion through such research results.

Antioxidant and Antibacterial Activities of Lactobacillus-fermented Artemisia annua L. as a Potential Fish Feed Additive (양어 사료첨가제로서의 유산균 발효 개똥쑥의 항산화 및 항균활성)

  • Lee, Ah-Ran;Niu, Kai-Min;Kang, Su-Kyung;Han, Sung-Gu;Lee, Bong-Joo;Kim, Soo-Ki
    • Journal of Life Science
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    • v.27 no.6
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    • pp.652-660
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    • 2017
  • Fermented medical herbs using Lactobacilli have attracted significant attention due to their enhanced biological activities. A traditional medicinal plant, Artemisia annua L., was fermented using a probiotic strain, L. plantarum SK3494. The strain was isolated from Artemisia princeps var. orientalis and molecularly identified through sequence similarities and phylogenetic tree analysis. The antioxidant activity of L. plantarum-fermented A. annua L. (LFA) was determined using the DPPH free radical scavenging assay. Cellular antioxidant activity of LFA was examined using the superoxide radical reduction assay in MAT-C cells. Total polyphenol contents (TPC) and flavonoid contents (TFC) of LFA were determined. The antibacterial activity of LFA against fish pathogens was also determined in this study. The viable cell number (9.38 log10 CFU/ml) and pH (4.1) results showed good adaptive ability of the selected strain during fermentation. LFA was found to have enhanced antioxidant activity compared to non-fermented A. annua L. (NFA) based on the DPPH assay. Cellular antioxidant activity was present in both LFA and NFA. After 24 hr and 48 hr of fermentation, the LFA also showed antibacterial activities against fish pathogens Photobacterium damselae subsp. damselae and Vibrio ichthyoenteri. These results suggest that L. plantarum-fermented A. annua L. may have potential as a feed additive in aquaculture.

Winterkill and Strategy of Golf Course Management: A Review (동절기 피해의 이해와 겨울철 골프장 관리: 리뷰)

  • Lee, Sang-Kook
    • Asian Journal of Turfgrass Science
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    • v.25 no.2
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    • pp.133-137
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    • 2011
  • Winterkill can be defined as any injury including freeze stress kill, winter desiccation, and low temperature disease to turfgrass plants that occurs during the winter period. The major damages from winterkill were low temperature kill, crown hydration, and winter desiccation. Low temperature kill is caused by air and soil temperature. Soil temperature affect more severe to turfgrass than air temperature because low soil temperature cause fetal damage to turfgrass crown. Crown hydration is a form of winter injury in which intercellular water within the plant freezes and causes physical injury to the cell membrane and wall. This is eventually resulted in dehydration of cell. Winter desiccation is the death of leaves or whole plants due to drought during the winter period. To reduce winterkill damage, cultivar selection is very important. If changing cultivar is not allowed, cold temperature hardiness needs to be increased by providing nutrients especially phosphorus and potassium in the late fall. Turf cover is effective way to reduce winterkill damage. Remaining snow is positive process to reduce winterkill damage by insulating soil temperature. The previous researches reported many materials as turf cover such as straw, polypropylene, polyester, and wood mat. Aeration and topdressing is one of the process against winterkill. Both methods are mainly conducted to reduce thickness of thatch layer. In recent, relatively new materials called black or winter topdressing sand are used to protect soil temperature from low air temperature and thaw ice crystal that may remain in soil.

Appropriateness Evaluation of Plug Seedling Cultivation for Replanting of Rehmannia glutinosa (Gaertn.) Libosch. ex Steud (보식용 지황의 플러그 육묘 재배를 위한 적정성 평가)

  • Lee, Sang Hoon;Kang, Seung Uk;Lee, So Hee;Koo, Sung Cheol;Hur, Mok;Jin, Mei Lan;Lee, Woo Moon;Park, Min Su;Kim, Yeon Bok;Han, Jong Won
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.3
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    • pp.202-207
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    • 2019
  • Background: Rehmannia glutinosa is a perennial herb belonging to the family Scrophulariaceae and is used in traditional oriental medicine. This study was conducted to evaluate the appropriateness of plug-seedling cultivation for replanting of R. glutinosa. Methods and Results: The seed rhizomes of Rehmannia glutinosa 'Jihwang 1' were harvested in the middle of March, 2018. Direct seedling cultivation was conducted after air curing for 3 days. The seedlings for transplanting were cultivated for 30 (30D), 45 (45D), and 60 days (60D). The mat formation rates of the 30D, 45D and 60D treatment seedling were 81.3%, 100%, and 100%, respectively. Of the tree treatment groups, flowering occurred only in the 45D and 60D treatment groups, and their flowering rates were $7.3{\pm}3.06%$ and $13.3{\pm}1.15%$, respectively. The agronomic characteristics and active components of the three treatment groups were evaluated in comparison with the DS1 (directly seeded group, control) and DS2 (directly seeded group, replanting) groups. As a result, the leaf length and leaf width of the 30D seedlings were higher than other treatment groups, but the number of leaves per plant was statistically the same. In addition, root diameter and yield of the 30D seedling were statistically the same as DS1. Moreover the catalpol contents of 30D, 45D, DS1 and DS2 group seedlings were 2.23%, 1.86%, 2.50% and 2.08%, respectively. Conclusions: Taken together, these findings suggest it is feasible to use the plug-seedling cultivation method for replanting of R. glutinosa. seedlings. However, further evaluation of the economic aspect is needed.

Machine Transplanting Cultivation with Infant Seedling in Rice Plant II. Effects of Raising Temperature, Duration and Nutritional Residue in Endosperm on Seedling Growth after Transplanting. (벼 어린모(유묘) 기계이앙 재배연구 II. 육묘온도, 육묘일수 및 배유양분잔존량이 이앙후 조기생육에 미치는 영향)

  • Yang, Won-Ha;Yun, Yong-Dae;Song, Moon-Tae;Lee, Moon-Hee;Lim, Moo-Sang;Park, Rae-Kyeong
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.4
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    • pp.434-439
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    • 1989
  • This study was carried out to investigate the minimum days for raising infant rice seedling with different temperature (day/night $^{\circ}C$ : 20/12, 25/18, 30/20) and to compare with seedling growth and rooting ability at 10 days after transplanting with endosperm-intacted and removed seedlings. The minimum days for raising infant rice seedling was shown differently with different temperatures, thus there were turned out by mat formation to be 10 days at 20/12$^{\circ}C$ (day/night), 8 days at 25/18$^{\circ}C$ and 6 days at 30/20$^{\circ}C$, respectively. Seedling height, leaf number and dry weight of top part at the minimum days for raising infant rice seedling were 8-12cm, 1.5-1.7 leaves and 6.9-7. 5cm per seedling, respectively. The seedling growth at 10 days after transplanting was better at high temperatures (25/18$^{\circ}C$) than low temperature (20/12$^{\circ}C$) at transplanting, and the growth of infant rice seedling with endosperm -intacted was better than that with endosperm-removed. This tedency was shown significantly in transplanting at 20/12$^{\circ}C$ (day/night) of 4 days seedling.

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Bloom of a green alga Bryopsis plumosa(Hudson) C. Agardh at Songji Beach, Haenam, Korea (한국 해남군 송지해변 녹조류 참깃털말 Bryopsis plumosa(Hudson) C. Agardh의 대량발생)

  • Hyun Il Yoo;Ji Woong Lee;So Mi Koh;In Ho Kim;Eun Kyoung Hwang
    • Korean Journal of Environmental Biology
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    • v.42 no.1
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    • pp.127-134
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    • 2024
  • The feathery branched green alga Bryopsis plumosa (Hudson) C. Agardh bloomed at Songji Beach, Haenam, Korea, in November 2022. A terrible stench was present on the beach, and the bottom was covered in a thick green mat of green algae. The alga was identified as B. plumosa, which is an opportunistic species currently distributed worldwide. Dissolved inorganic nitrogen (DIN) and dissolved inorganic phosphorus (DIP) were analyzed in the bloomed area and compared with those of other vicinities. The DIN and DIP concentrations were 1.067 and 0.461 mg L-1, respectively, which were 6 to 19 times higher than the average concentrations on southwest coasts, including Haenam. The B. plumosa bloom at Songji Beach in November 2022 appears to have depended on DIN and DIP concentrations in seawater. In this study, we report on the mass occurrence of B. plumosa, which appeared for the first time in Korea. This occurrence was found to be closely related to the concentration of nutrients in seawater. Therefore, it is necessary to manage the concentration of nutrients on land flowing into coastal waters to control green algal blooms such as Bryopsis.

Studies on the Use of Radioisotope Tracer Technique to Investigate and Improve the Root Activities in Rice Plant (II) - Effect of Application of Several Kinds of Phosphorous Fertilizer - (방사성동위체(放射性同位體) 도입(導入)과 그 추적기술(追跡技術)에 의(依)한 수도근계(水稻根系) 활성상(活性相)의 해명(解明)과 개선(改善)에 관(關)한 연구(硏究) - 인산질(燐酸質) 비료(肥料)의 비종별(肥種別) 시용효과(施用效果)에 대(對)하여 - (제2보)(第2報))

  • Ahn, Hak-Soo;Chung, Hee-Don;Ahn, Jon-Sung;Ro, Jun-Chong;Kim, Kyu-Won;Shim, Sang-Chil
    • Applied Biological Chemistry
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    • v.15 no.1
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    • pp.85-92
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    • 1972
  • The field experiment was performed to investigate the effects of various kinds of phosphorus fertilizers such as double superphosphate, fused magnesium phosphate and Simagcarin (both the Kyun-gi Chemical Co, products) on the physiological roles in development of root system, growth and yield compositions of rice plant. Radioactive phosphoric acid $(H_3\;^{32}PO_4)$ was applied to measure the root activity. 1. The number of total tillers was significantly increased in double superphosphate plots, but the rate of fruitful tillers was more numerous in the fused magnesium phosphate and the Simagcarin plots than that of the other plots. 2. The grain yield was much more obtained in the fused magnesium phosphate and Simagcarin plots (no significant difference were found between both of plots) than the double superphosphate and control plots. It seemed due to the increasing of seedbearing rate and number of fruitful tillers. 3. In double superphosphate plots, root system was mostly developed near topsoil areas, but fused magnesium phosphate and the Simagcarin plots, root system was uniformly distributed from topsoil to subsoil areas. 4. As the results of those experiments, fused magnesium phosphate and Simagcarin was demonstrated to be soil amendmentical materials rather than the phosphorus fertilizers, especially in low productive paddy soils which lack the special mineral nutritions.

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Studies on the Breeding of the Response to short photoperiod, Fiber weight, and Qualitative characters and of the Associations Among these characters in Kenaf (섬유용양마의 육종에 관한 연구 -단일반응성과 섬유종의 유전 및 연소)

  • Johng-Moon Park
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.4 no.1
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    • pp.115-124
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    • 1968
  • It was shown that the most desirable characters for kenaf are high-fiber weight and moderately early maturity. Therefore, the objectives of this research on this crop is to find varieties possessing these characteristics. The experiments covered in this report provided new information relative to segregation, mode of inheritance, estimate of the number of genes involved in fiber weight and their response to short day length of 10 hours and the qualitative characters, such as, color of stem, capsule, petiole and shape of leaves. The associations which exist among these characters are also indicated. Fiber weight per plant, days to flowering, Stem color, Petiole color, Capsule color, and shape of leaves were studied in parental, $F_1$.$F_2$and backcross populations of a cross between Dashkent, a low-fiber weight but early maturing kenaf variety, and G 38 F-1, a high-fiber weight but late maturing kenaf variety. Crosses were made using the varieties, Dashkent and G 38 F-1 as parents. The Dashkent parent had the following characteristics: green stems, capsules and petioles and lobed shaped leaves; 105.8234 mean-days to flowering in the field, and 106.9222 mean-days under 10 hours short day treatment. The other parent, G 38 F-1 had red stems yellow capsules and red petioles and unlobed shaped leaves; 149.8921 mean-days to flowering in the field, and 62.3684 mean-days under 10 hours short day treatment. Both of the parents, $F_1$, $F_2$, $BC_1$ ($F_1$ X Dashkent, ) and $BC_2$($F_1$ ${\times}$ G38F-1) of the kenaf cross were grown at the Crops Experiment Station, Suwon, Korea in 1965. Color of stems, petioles and capsules, and shape of leaves were noted to be simply inherited as a single factor. Red stem color was dominant over green stem color, red petiole color was dominant over green petiole, lobed shaped leaves were dominant over unlobed shaped leaves and yellow capsules were dominant over green capsule. It was, also, noted that the factor for color of petiole was linked with the factor for shape of leaf with a 11.9587 percent recombination value, however no interaction or linkage were found among the color of stem and capsule color. Using Powers partitioning method, theoretical means and frequency distributions for each population, the days to flowering were calculated with the assumption that two gene pairs were involved. The values obtained fitted the theoretical values. In general this would indicate that Dashkent and G 38 F -1 were differentiated by two gene pairs. Heritability values were calculated as the percent of additive genetic variance. Heritability value of days to flowering, 89.5% in the broad sense and 79.91% in the narrow sense, indicated that the selection for this character would be effective in relatively early generations. Particularly, high positive correlations were found between days to flowering and the color of petioles and shape of leaves. However, there was no relation between days to flowering and capsule color nor between these and stem color. On the basis of the results of this experiment there is evidence that the hereditary factor for shape of leaves and the color of petioles is linked with an effective factor or factors for the characters of days to flowering. The association was sufficiently close to offer a possible simple and efficient means of selection for moderately early mat. uring plants by leaf shape and petiole color selection. Again using Powers partitioning method the frequency distribution for each population to the fiber weight were calculated with the assumption that two gene pairs, AaBb, were involved. Both phenotypic and genotypic dominance were complete. The obtained value did not agree with the theoretical value for $F_2$ and $BC_1$ ($F_1$ ${\times}$ Dashkent.) It seems that Dashkent and G 38 F-1 were differentiated by two major gene pairs but some the other minor genes are necessary. It is certain that the hereditary factor for shape of leaves and color of petioles is linked with an effective factor or factors for fiber weight. Also, high. yielding plants with moderately early maturity were found in the $F_2$ population. Thus, simultaneous selection for high-fiber yield and moderately early maturing plants should be possible in these populations. Phenotypic and genotypic correlation coefficients between fiber weight per plant and days to flowering, stem height and stem diameter were calculated. In general, genotypic correlations are higher than the phenotypic correlation. The highest correlation is found between stem height and fiber weight per plant (0.7852 in genotypic and 0.4103 in phenotypic) and between days to flowering and fiber weight per plant (0.7398 in genotypic and 0.3983 in phenotypic.) It was also expected that the selection of high stem height and moderately early maturing plants were given the efficient means of selection for high fiber weight.

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