• Journal of Microbiology and Biotechnology
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• v.33 no.1
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• pp.15-27
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• 2023

The incidence of melioidosis cases caused by the gram-negative pathogen Burkholderia pseudomallei (BP) is seeing an increasing trend that has spread beyond its previously known endemic regions. Biofilms produced by BP have been associated with antimicrobial therapy limitation and relapse melioidosis, thus making it urgently necessary to understand the mechanisms of biofilm formation and their role in BP biology. Microbial cells aggregate and enclose within a self-produced matrix of extracellular polymeric substances (EPSs) to form biofilm. The transition mechanism of bacterial cells from planktonic state to initiate biofilm formation, which involves the formation of surface attachment microcolonies and the maturation of the biofilm matrix, is a dynamic and complex process. Despite the emerging findings on the biofilm formation process, systemic knowledge on the molecular mechanisms of biofilm formation in BP remains fractured. This review provides insights into the signaling systems, matrix composition, and the biosynthesis regulation of EPSs (exopolysaccharide, eDNA and proteins) that facilitate the formation of biofilms in order to present an overview of our current knowledge and the questions that remain regarding BP biofilms.

• ALGAE
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• v.19 no.3
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• pp.217-226
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• 2004

The present study is to examine the water quality and to analyze the plank tonic and benthic phytoplankton communities at 6 stations in the Dong River and its tributary streams of Kangwon Province from November, 2001 to March, 2002. During the studies, water temperature ranged from 3.5 to 12.8℃; pH, 6.5-7.9; DO, 9.29-11.36 mg·l^(-1); BOD_5, 0.20-2.38 mg·l^(-1); TN, 1.2842-3.1871 mg·l^(-1); TP, 0.0052-0.0576 mg·l^(-1); and SS, 0.85-9.62 mg·l^(-1). The standard of water quality according to the Korean Environmental Preservation Law showed the first class except St. 6 through the survey. Six taxa of plank tonic phytoplankton identified were poor flora in November, 2001. The representative species frequently observed were the diatoms Achnanthes lanceolata, Achnanthes minutissima, Cymbella minuta, Cymbella parva, Diatoma vulgare, Fragilaria construens, Gomphonema pervulum, Navicula cryptocephala, Nitzschia palea., Scenedesmus sp. of green algae and Stephanodiscus hantzschii. Monthly dominant species of phytoplankton were Achnanthes lanceolata and A. minutissima in both February and March, 2002, but the blue-green algae, Oscillatoria sp. and the diatom, Stephan discus hantzschii were predominant at some stations in March. Phytoplankton standing crops ranged from 9.84 ${\times}$ 10$^3$ to 3.56 ${\times}$ 10$^4$ cell·l$^{-1}$ in November and 1.68-2.99 ${\times}$ 10$^5$ cell·l$^{-1}$ in February, while it changed 4.52-8.01 ${\times}$ 10$^5$ cell·l$^{-1}$ at St. 1, 2, 3 and 1.03-1.71 ${\times}$ 10$^6$ cell·l$^{-1}$ at St. 4, 5, 6 in March. Benthic phytoplankton communities was composed of 38 taxa in November, 31 taxa in February and 23 taxa in March. It showed a contrary tendency to planktonic phytoplankton diversity. Benthic diatoms which were more than 25% of the total populations were Cymbella turgida, Diatoma vulgare, Cocconeis placentula, Navicula cryptocephala var. intermedia in November; Achnanthe lanceolata, Cocconeis placentula, Achnanthes minutissima in February; and Achnanthes lanceolata and Cyclotella meneghiniana in March.

• The Korean Journal of Food And Nutrition
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• v.24 no.4
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• pp.657-663
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• 2011

$Bacillus$ $cereus$ is widely distributed on various foods and is known to cause clinical infections, food poisoning toxin induced diarrhea and vomiting. In this study, $B.$ $cereus$ group detected and analyzed rice, rice bran, and biofilm characterization of $B.$ $cereus$ confirmed. $B.$ $cereus$ was identified in approximately 34.6% of brown rice and 50.0% of rice bran. $B.$ $thuringiensis$ was detected in 3.9% of brown rice and 23% of rice bran, and $B.$ $mycoides$ was isolated from rice bran. The microtiter plate assay detected differences in biofilm-forming ability among $B.$ $cereus$ group isolates. Biofilm of $B.$ $cereus$ seemed to increase the MIC values of antimicrobial agent and antibiotic compounds compared with planktonic cells. Therefore, sufficient attention should be given to good manufacturing practice and good agriculture practice to avoid contamination of $B.$ $cereus$ group raw material including rice.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.3 no.2
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• pp.10-23
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• 2000

The purpose of this research was to investigate water spaces such as marshes and watercourses, especially focusing on methods to establish eco-ponds. Many aqua-ecosystems have been filled up or destroyed in the process of rapid industrialization and urbanization in the cities. This inquiry was done by comparing, studying and analyzing existing ponds and studying documents on the introduction and methods of making eco-ponds. Existing ponds were put into three categories as the either eco-pond, man-made/natural ponds, or man-made ponds. Three instances were selected for each category, totaling nine. Data on the size and shape, depth, waterproofing techniques, water supply, plants and planting plan, and the maintenance of the ponds was collected and compared. The following are conclusions based on comparing, studying and analyzing these existing ponds, and studying documents on the introduction and methods of making eco-ponds. ${\cdot}$ It is noteworthy that different environments and depths must be provided because these are main variants contributing to attracting plants in the ponds. ${\cdot}$ In these places plants emerged, such as, floating-leaved plants, free-floating planktonic plants, submerged plant etc, in the different water level zones, once these various conditions were provided. ${\cdot}$ Various spaces can be used for shelter, places for relaxation and habitation. ${\cdot}$ After establishing a pond, routine maintenance is necessary for preventing eutrophication. However the minimal possible management should be done to maintain a natural status.

• Ocean and Polar Research
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• v.42 no.3
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• pp.249-261
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• 2020

This study details grain-size distributions (GSDs) of carbonate and biogenic opal fractions of the sediments retrieved from the South Korea Plateau in the East Sea and draws paleocanographic implications from them. The opal-fraction GSDs show fine modes of 10.3 ㎛ and coarse modes of 102.5 ㎛ on average. The fine-mode grains of opal fractions mainly consist of small diatoms and radiolarians including their broken frustules, while the coarse-mode grains are mostly comprised of large warm-water diatoms and radiolarians. Significant positive correlation between opal contents and abundances of the coarse-mode GSDs in the total GSDs suggests that the abundances of the coarse-mode GSDs were controlled by the increased surface productivity of warm-water diatoms during interglacial stages. The carbonate-fraction GSDs show fine modes of 2.4 ㎛ and coarse modes of 99.1 ㎛ on average. The fine-mode grains mainly consist of coccolithophores, while the coarse-mode grains are mostly comprised of intact or broken planktonic foraminifera. The abundances of coarse-mode and fine-mode GSDs were not correlated with carbonate contents, suggesting a complex control exerted by both the degree of carbonate dissolution and the productivity of coccolithophores on the carbonate-fraction GSDs.

• ALGAE
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• v.26 no.2
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• pp.153-165
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• 2011

Species of the diatom genus Chaetoceros with special intercalary setae are uncommon. For this study, we collected Chaetoceros species from August 2008 to September 2009 in Korean coastal waters and examined the ultra structures of the Chaetoceros species C. coarctatus, C. compressus var. hirtisetus, C. contortus, C. diversus, and C. messanensis, using light and scanning electron microscopy. C. coarctatus, in the subgenus Phaeoceros, showed longer and stronger spines than those found in other species. C. coarctatus and C. diversus had special intercalary setae with spines in straight arrangements, whereas C. compressus var. hirtisetus, C. contortus, and C. messanensis had special intercalary setae with spines arranged in spirals. The setae of C. coarctatus had spines that were robust toward the tips and, overall, longer and stronger than were those of other species. C. coarctatus and C. diversus were straight, and C. compressus var. hirtisetus, C. contortus, and C. messanensis spiraled. C. messanensis had two types of special intercalary setae, both forked: 1 with spines in spirals and 1 lacking spines. We did not find spines on the anterior part of divergent point of the special intercalary setae of C. messanensis. Foramina shapes of these 5 Chaetoceros species varied as follows: very small or no foramina in C. coarctatus, relatively wide and slightly centrally constricted foramina in C. compressus var. hirtisetus and C. contortus, quite narrowly slitted or no foramina in C. diversus, and lanceolate or hexagonal foramina in C. messanensis. We found rimoportula in both intercalary and terminal valves of C. coarctatus, but C. compressus var. hirtisetus, C. contortus, C. diversus, and C. messanensis only had rimoportula in terminal valves. In addition, C. compressus var. hirtisetus and C. contortus were new to Korean coastal waters.

• 한국해양학회지
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• v.27 no.1
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• pp.11-18
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• 1992

To study on feeding behavior of copepods, Calanus sinicus and Acartia clausi(A. omorii), variations of filtering rate and rate of food intake with the changes of phytoplankton concentrations and time progress were measured in laboratory. Filtering rates of the two species of copepod declined with increasing of the food concentration and the averaging filtering rates C. sinicus and A. clausi were 2.42m/ copepod/hr., 3.37 ml/copepod/hr. respectively. And the rates of food intake increased with food concnetration up to a critical concentration. At higher concentrations, the rates of food intake were somewhat variable but kept the constance levels. Filtering rates of copepods with time progress didn't show diel feeding rythms and generally decreased. But filtering rate of A. Clausi increased by the increment of feeding in a few hours. consequently, two copepods showed similar tendency in the rate of food intake and filtering rate with phytoplankton concentrations. However, the measured values appeared differently. And the two species of copepod with time progress showed the non-continuous feeding behavior.

• 한국해양학회지
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• v.12 no.2
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• pp.75-81
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• 1977

Distribution of drifting larvae of Anadara broughtnoii SCHRENCK was studied based on the planktonic sampling which has been collected in fifteen sampling areas of southern coast of Korea and Ulsan Bay during summer season from 1973 to 1977. Vertical and horizontal occurrence was analyzed related to the environmental factors such as surface water temperature, current velocity and depth of water column. High density of the larvae was observed in the Chinhae Bay which included the sampling areas Rampo, Sockcheon, Majeon, Changpo, Dangdong, Bedun, Changchoa, and Wonmun. Maximum occurrence of the farvae was accompanied with the highest water temperature of the summer season, and it was usually August when the water temperature was over 27$^{\circ}C$. In August, 1975, the highest density of the farvae was observed, when the mean surface water temperature was the highest compared to those of other years. The first appearence of the drifting larvae was also related to the surface water temperature. Each year the larae begin to appear from the late July and the ready-to-fall larvae appear in abundance from the mid-August. Vertical distribution patterns of the larvae are closely related to the depth of the water column as well as to the current velocity. In shallow water the larvae tend to aggregate in the bottom layer, while they are diffused to some extent in deep water. In shallow water column ( 8m) more or less 75% of the total larvae individuals was observed in the lower 4m layer and in deep water column ( 16m) only 45% of those was found in the lower 4m layer. In the water of lower velocity a large fraction of the larvae population is distributed in the lower depth layer.

• Journal of Microbiology and Biotechnology
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• v.29 no.12
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• pp.1916-1924
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• 2019

Outbreaks of staphylococcal food poisoning (SFP) causing serious human diseases and economic losses have been reported globally. Furthermore, the spread of Staphylococcus aureus with increased resistance to multiple antimicrobial agents has become a major concern in the food industries and medicine. Here, we isolated an endolysin LysSAP8, as one of the peptidoglycan hydrolases, derived from the bacteriophage SAP8 infecting S. aureus. This endolysin was tagged with a 6×His at the C-terminal of the target protein and purified using affinity chromatography. LysSAP8 demonstrated lytic activity against a broad spectrum of bacteria, which included a majority of the staphylococcal strains tested in this study as well as the methicillin-resistant S. aureus (MRSA); however, no such activity was observed against other gram-positive or gram-negative bacteria. Additionally, LysSAP8 could maintain bactericidal activity until 0.1 nM working concentration and after heat treatment at 37℃ for 30 min. The ability of LysSAP8 to lyse cells under varying conditions of temperature (4-43℃), pH (3-9), and NaCl concentrations (0-1,000 mM), and divalent metal ions (Ca²⁺, Co²⁺, Cu²⁺, Mg²⁺, Mn²⁺, Hg²⁺, and Zn²⁺) was examined. At the optimized condition, LysSAP8 could disrupt approximately 3.46 log CFU/ml of the planktonic cells in their exponential phase of growth within 30 min. In this study, we have suggested that LysSAP8 could be a potent alternative as a biocontrol agent that can be used to combat MRSA.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.16 no.4
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• pp.316-329
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• 1983

The formation and structure of the shelf front in the eastern part of the Yellow Sea are studied on the basis of oceanographic data collected in August, 1982 and February, 1983. This paper also describes the distribution of planktonic organisms of the shelf front. In summer the shelf front is formed in the area ($126^{\circ}02^'E-126^{\circ}05^'E$) ca. 20 miles from the shore at the depths of 15-25 m. in winter, however, no distinct shelf front is formed. Based on the cluster analysis of surface phytoplankton the species composition shaws a discontinuous pattern in the vicinity of the shelf front in summer, 1982. A similar trend is observed in distribution of some copepod species in winter, l983.