• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.56-56
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• 2023

The cone of Pinus rigida × Pinus taeda (PRT), a plant in the Pinaceae family, has long been used in traditional medicine to treat hemostasis, bruises, and burns. Previous research has shown that regulating oxidation-reduction reactions in reactive oxygen species can help inhibit melanogenesis, the process of melanin synthesis, which is a common target for addressing hyperpigmentation. Inhibiting tyrosinase is also known to be effective in this regard. Based on these findings, we conducted an investigation into the inhibitory effect of the ethyl acetate fraction of PRT (ERT) on melanogenesis in B16 F10 cells. We know that the expression levels of melanin biosynthesis-related proteins, including tyrosinase, TRP-1, and TRP-2, are regulated by MITF (microphthalmia-associated transcription factor) and cAMP, with cAMP affecting the activity of protein kinase A (PKA). PKA can reduce melanogenesis, and CREB reduces the phosphorylation of melanin-producing enzymes. In addition, the MAPK signaling pathway, composed of ERK, JNK, p38, and other factors, is also known to play a role in the inhibition of melanogenesis in melanocytes. Our immunoblotting results showed that ERT inhibited the expression of melanin production-related proteins (tyrosinase, TRP-1, TRP-2, and MITF) that were significantly increased by a-MSH treatment to promote melanin production. Furthermore, the phosphorylation levels of factors related to cAMP/PKA/CREB and MAPK signaling pathways were significantly reduced without affecting the total form. In conclusion, we believe that treatment with ERT can inhibit melanin synthesis by modulating the phosphorylation of cAMP/PKA/CREB and MAPK signaling pathways at the cellular level. These findings suggest the potential of ERT as a raw material for functional cosmetics and pharmaceuticals, thanks to its antioxidant activity and ability to inhibit melanogenesis. We thought that these findings of ERT as a natural plant resource will inspire further research and development in this area.

• Korean Journal of Environment and Ecology
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• v.26 no.4
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• pp.559-575
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• 2012

This study, targeting Namhan Mountain Fortress which was designated as a No. 57 national historic site and placed on the World Heritage Tentative List in 2010, was intended to identify the change of vegetation structures by reviewing past references, pictures, research data and additionally conducting a site survey. Also, it was designed to draw up measures for restoring vegetation suitable for historically and culturally valuable Namhan Mountain Fortress. According to the biotope mapping of study site, Quercus spp. forest distributed a greatest part of area with 40.8% of $2,611,823m^2$. Pinus densiflora forest, highly likely to go through ecological succession, was dispersed in the whole region of Cheongryangsan, the area from West Gate to North Gate and the ranges between South Gate to Cheongryangsan with taking 16.5%. Pinus densiflora forest with a low probability of succession amounted to 4.7% and was dispersed mainly in the forest behind Namhansan elementary school. Pinus densiflora going on the ecological succession is distributed a portion of 2.9%. And the currently dying out Pinus densiflora forest amounted to 2.1%. As a result of analysis of the vegetation structure for 19 years, the succession from Pinus densiflora forest to Pinus densiflora and succession from Quercus spp. mixed forest to Quercus spp. forest to Carpinus laxiflora forest were predicted. Additionally, Quercus spp. expanded its dominance over time. According to the characteristics of each classified zone, the site was categorized into $553,508m^2$ area of Pinus densiflora forest area for the landscape maintenance, $114,293m^2$ area of Pinus densiflora forest area for the landscape restoration, $205,306m^2$ area of Pinus densiflora forest area for the disclimax, and $1,169,973m^2$ area of Pinus densiflora forest area for inducing ecological succession.

• Journal of Forest and Environmental Science
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• v.16 no.1
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• pp.42-49
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• 2000

This study deals with the structure of Carabid beetle community surveyed in different stands. Species richness and abundance of Carabid beetles were 1,162 individuals, 23 species, 10 genera in the Pinus densiflora stand, 418 individuals, 23 species, 11 genera in the Pinus koraiensis stand, 782 individuals, 25 species, 11 genera in the Larix leptolepis stand, 1,383 individuals 25 species, 12 genera in the Quercus variabilis stand. Dominant species were Synuchus cycloderus(19.8%), Synuchus nitidus(16.6%), Pterostichus pertirax(16.2%) in the Pinus densiflora stand. Pterostichus sp.1(27.9%), Synuchus nitidus(24.4%), Synuchus cycloderus(10.2%) in the Pinus koraiensis stand, Synuchus sp.1(40.0%), Synuchus nitidus(9.3), Pterostichus sp.1(7.4%) in the Larix leptoepis stand. Pterostichus sp.2(17.4%), Synuchus cycloderus(14.4%). Pterostichus pertirax(14.1%) in the Quercus variabili stand. Shannon species diversity index(H') in the Pinus densiflora, Pinus koraiensis, Larix leptolepis and Quercus variabilis stand were 2.11, 2.25, 2.23 and 2.32 respectively and Evenness index(J') were 0.64, 0.72, 0.69 and 0.72, respectively. Community similarity index showed the lowest value (0.66) between Quercus variabilis stand and Pinus koraiensis stand and the highest value (0.83) between Pinus densiflora stand and Larix leptolepis stand.

• Journal of the Korean Wood Science and Technology
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• v.12 no.3
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• pp.25-34
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• 1984

This experiment was carried out to investigate the effects of monosaccharides extracted by saturated portland cement solution on the cement setting in comparision with the inhibitory index (I) of each lignocellulosic-cement system. The wood species which have been widely reforested in Korea, Populus alba-grandulosa, Larix leptolepis, Abies holophylla, Pinus koraiensis, Pinus rigida, Pinus densiflora and agricultural wastes of rice husk and rice stalk were used at this study. The wood meal, 0.50g on dry weight basis, through 0.83 mm(20 mesh) and retained on 0.35mm (40 mesh) screen was extracted by 25 ml saturated portland cement solution and the pH of saturated portland cement solution Was 12.7. To eliminate cation exsisting in the extracted solution, the cation exchange column was used (Fig. 4). Afterwards the extracted monosaccharides were reduced into alditols with sodium borohydride and analyzed by the gas-liquid chromatography for xylan, mannan, arabinan, galactan, gluean. The heat of cement hydration for lignocellulosic-cement system was measured in Dewar flask (Fig. 2). And then the inhibitory indices were calculated from maximum hydration temperature, time and maximum slops of hydration curve of ligno cellulosic-cement systems. The results obtained were as follows; (1) The inhibitory index of pines-Pinus rigida (I=29.33) and Pinus densiflora (I=35.76), were lower than that of poplar-Populus alba-glandulosa (I=41.48), and the index of Larix ieptoiepis (I=73.00) was the highest among eight lignocellulosic-cement systems, and accordingly both Pinus rigida and Pinus des(flora were seemed to be good wood species for wood-cement composite manufacture. (2) In case of Pinus rigida, the inhibitory index was 29.33 and the ratio of the hexoses to the pemoses was 6.04 and in case of Larix leptolepis, the index and the ratio were 73.00 and 35.19, respectively. Therefore the inhibitory index increased with increasing the ratios of the hexoses to the pentoses. (3) The richer amount of xylose and mannose in species caused decreasing the slops of the hydration curve of the lignocellulosic-cement system, prohahly due to the chemical adsorption of the acetyl groups in the hemicellulose on the surface of cement grains. (4) The amoun of xylose and mannose were significant to the inhibitory index of each lignocellulosic-cement system but any specific relation between the amount of glucose and inhibitory index was not found.

• Journal of the Korean Wood Science and Technology
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• v.14 no.4
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• pp.40-46
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• 1986

Change of color of woods is a trouble frequently found in decorative wood products. In this paper, studies were carried out on discoloation sensitivities of wood specimens to iron (0.1%, $FeCl_3{\cdot}6H_2O$), alkali (pH12.0, NaOH), acid (pH 1.0 $C_2H_2O_4$) and exposing to sunlight (40 hrs.). Four soft-woods and eleven hard-woods grown in Korea were used in this test. The results obtained were as follows: 1. In iron stain, strong discolored wood species were Larix leptolepis, Quercus mongolica. Zelkova serrata and Prunus yedoensis, and light discolored were Paulownia lomentosa and Pinus rigida. 2. In alkali stain. light discolored wood species were Abies holophylla, Kalopanax pictum, Populus ${\times}$ albaglandulosa, Acer mono, Pinus rigida, Pinus densiflora, Quercus mongolica, Betula platyphylla var. japonica, Prunus yedoensis, Zelkova serrata, Populus euramericana, Pinus koraiensis and Paulownia tomentosa, but there was no wood species of strong discolored compared with the color difference values of other reports. 3. In acid stain, light discolored wood species were Pinus rigida, Paulownia tomentosa. Pinus koraiensis, Quercus mongolica, Kalopanax piclum and Acer mone, but there was no strong discolored species compared with the color difference values of other reports. 4. In exposing to sunlight, strong discolored species were Larix leptolepis, Acer mono, Paulownia tomentosa, Populus euramericana, Pinus densijlora, Robinia pseudoacacia, Abies holophylla, Populus ${\times}$ albaglandulosa, Zelkova serrata, Prunus yedoensis and Pinus rigida, and light discolored was Alnus japonica. In general, it was shown that Korean wood species were susceptible to change of color by exposing to sunlight and iron stain compared with by alkali stain and acid stain.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.929-933
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• 2010

According to the IPCC guideline (2006), uncertainty assessment is very important in terms of the greenhouse gas inventory. Therefore, the purpose of this study is to estimate the basic wood density (BWD) and its uncertainty for Pinus densiflora in Korea. In this study, Pinus densiflora forests were divided into two ecotypes which were Gangwon and Jungbu regions. A total of 33 representative sampling plots was selected to collect sample trees after considering the tree ages and DBH distributions. The BWD showed statistically no difference between age classes based on IPCC's classification. While, it showed statistically difference(pvalue=0.0017) between eco-types. The BWD and uncertainty was 0.396(g/$cm^3$) and 12.9(%) for Pinus densiflora in Gangwon, while it was 0.470(g/$cm^3$) and 3.8(%) for Pinus densiflora in Jungbu. The values of the BWD uncertainty for Pinus densiflora were more precised than the values given by the IPCC guideline.

• Korean Journal of Agricultural Science
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• v.9 no.2
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• pp.494-506
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• 1982

The community structure and dynamic succession of forest were determinated on the quadrat plots selected randomly at the Mt. Bomun. The plot size was $20{\times}20m$, and analysis of vegetation and soil 'data we re adapted reciprocal averaging(RA) ordination method. 1. The numbers of tree species were twenty, and dominant species were Quercus variabilis, Pinus densiflora, Alnus hirsuta, and Pinus rigida. 2. The forest of Mt. Bomun was found to be composed of Pinus rigida community and Quercus-Pinus community through RA ordination method. 3. The community of Quercus variabilis was assumed to be dominant in the succession of forest. 4. The soil factors, such as total-N, $NO_3$, $NH_4^+$, $P_2O_5$, organic matter, C. E. C., pH, $K^+$, $Ca^{{+}{+}}$ and $Mg^{{+}{+}}$, were analyzed. Total-N, $NO_3^-$, $NH_4^+$, and $P_2O_5$ were assumed to be the main factors of the soil.

• Journal of Korean Society of Forest Science
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• v.101 no.3
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• pp.509-516
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• 2012

In this research, it was intended to examine the vulnerability of Pinus rigida to climate changes, a major planting species in Korea. For this purpose, the distribution of Pinus rigida and its changes caused by climate changes were estimated based on the 'A1B' climate change scenario suggested by IPCC. Current distribution of Pinus rigida was analyzed by using the $4^{th}$Forest Type Map and its potential distribution in the recent year (2000), the near future (2050) and the further future (2100) were estimated by analyzing the optimized ranges of three climate indices - warmth index(WI), minimum temperature index of the coldest month (MTCI) and precipitation effectiveness index(PEI). The results showed that the estimated potential distribution of Pinus rigida declines to 56% in the near future(2050) and 15% in the further future (2100). This significant decline was found in most provinces in Korea. However, in Kangwon province where the average elevation is higher than other provinces, the area of potential distribution of Pinus rigida increases in the near future and the further future. Also the result indicated that the potential distribution of Pinus rigida migrates to higher elevation. The potential distributions estimated in this research have relatively high accuracy with consideration of classification accuracy (44.75%) and prediction probability (62.56%).

• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.328-336
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• 1993

This study was conducted to investigate sensitivity of tree seedlings to $SO_2$. Visible injury symptoms and changes of ethylene production were investigated in tree seedlings with the fumigation of $SO_2$ in gas chamber 4 hours a day for six days. The symptoms of visible injury did not appear below 0.5ppm level of $SO_2$ exposure but a change of visible injury with the passage of time appeared at 1.5 and 2.5ppm in all seedlings. With the higher the concentration and/or the longer exposure of $SO_2$ the visible injury symptoms on leaves increased in all seedlings. The sensitivity of seedlings to $SO_2$ was the highest in Liriodendron tulipifera followed by Pinus strobus, Ginkgo biloba, Pinus densiflora and Pinus koraiensis. The amount of ethylene production was more at 1.5 and 2.5ppm of $SO_2$ exposure than at 0.5ppm and the peak time of it came faster at higher levels. The amount of ethylene production was significantly different among tree seedlings. It showed a higher at production of ethylene in Liriodendron tulipifera compared to Ginkgo biloba and the ethylene production of Pinus trees to $SO_2$ were the highest in Pinus strobus followed by Pinus densiflora and Pinus koraiensis. In needle of Pinus strobus the ethylene production increased with the increasing rate of visible injury until the injury rate of 40-50% and than decreased with the increasing rate of visible injury since the rate of 50%.

• Journal of the Korean Wood Science and Technology
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• v.37 no.3
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• pp.200-207
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• 2009

As an effort to utilize the pine woods damaged by pine wilt disease, this study analyzed the density of pine wood nematode and compressive strength of damaged trees treated through soaking and fumigating. The density of nematode in Pinus densiflora and Pinus thunbergii woods soaked in wood vinegar and nematicide, and in seawater reduced greatly in 21 days and in 30 days, respectively. When reextracted nematode was injected artificially into healthy trees, infection was not observed after the lapse of six months, and the mean density of Pinus densiflora and Pinus thunbergii was $0.47g/cm^3$ and $0.54g/cm^3$, respectively, and their compressive strengths were $304kgf/cm^2$ and $363kgf/cm^2$, respectively. As to change in the density of pine wood nematode after fumigating, pine wood nematode was detected until the third month in both species, and those detected afterwards were found to be similar nematodes. After 24 months, the mean densities of Pinus densiflora and Pinus thunbergii were $0.54g/cm^3$ and $0.52g/cm^3$, respectively, and their compressive strengths were $353kgf/cm^2$ and $370kgf/cm^2$, respectively.