• Journal of Life Science
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• v.19 no.8
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• pp.1132-1138
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• 2009

We investigated the physicochemical stabilities and biological activities of ethanol- extracted pigment from marine bacterium Pseudoalteromonas psicida TA20. This bacterial pigment was very stable at a pH range of between 4.0 and 8.0 at a temperature below $40^{\circ}C$. In the presence of light, the pigment was also very stable, showing more than 90 percent remaining absorbance during 14 days at $25^{\circ}C$. The stability of the pigment, when metal ions were present, showed higher stability in all examined metal ions except for $Al^{3+}$ and $Cu^{2+}$, especially in the presence of $Fe^{2+}$. This pigment showed higher stability than other pigment extracts reported. The pigment has free-radical scavenging (3,495 ${\mu}g/ml$) activity and 44% antioxidant protective effect against DNA damage of human lymphocyte cells at a concentration of 10 ${\mu}g/ml$. The results indicate that the bacterial pigment produced a significant reduction in oxidative DNA damage. The pigment also showed antimicrobial activity against major food poisoning bacteria. Therefore, these results suggest that this bacterial pigment could be used as a natural colorant in the food industry, having the advantages of antioxidant and antimicrobial activities.

• ALGAE
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• v.18 no.1
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• pp.83-88
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• 2003

• Journal of Microbiology
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• v.40 no.3
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• pp.234-236
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• 2002

A blue pigment-producing bacterium, Vogesella indigofera, was isolated and quantified for the relationship between its synthesis of a blue pigment and exposure concentrations of $Cr^{6+}$. The concentration of $Cr^{6+}$ and the percentage of blue colonies on agar plates was negatively correlated ($r^{2}$ =-0.8683). Critical concentrations inhibiting bacterial pigment production were found to be between 100-150 $\mu\textrm{g}$ $Cr^{6+}$/ml on agar plates and 200-300 $\mu\textrm{g}$ $Cr^{6+}$/ml in liquid culture. As the blue color is characteristic and easily observable, the bacterium Vogesella indigofera may have potential applications in the detection and monitoring of environmental pollution.

• Journal of the Korean Society of Safety
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• v.23 no.6
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• pp.91-99
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• 2008

The identification of thermal hazards associated with a process such as heats of reaction and understanding of thermodynamics before any large scale operations are undertaken. The evaluation of thermal behavior with operating conditions such as a reaction temperature, stirrer speed and reactants concentration in neutralization process of pigment plant are described. The experiments were performed by a sort of calorimetry with multimax reactor system The aim of the study was to evaluate the results of heat of reaction in terms of safety reliability to be practical applications. It suggested that we be proposed safe operating conditions and securities for accident prevention on reactor explosion through this study.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2020.06a
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• pp.167-168
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• 2020

In this study, we attempted to present a method for diagnosing the depth of the early frost damage concrete using organic pigments under the cold weather. As a result, it is considered that the organic pigment did not penetrate into the voids of the concrete that had been damaged by the early frost damage and only the surface was adhered. Therefore, when fine particles that can be melted in water and pass through voids are used, it is analyzed that it can penetrate damaged part of the concrete.

• Journal of the Korean Graphic Arts Communication Society
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• v.21 no.1
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• pp.1-10
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• 2003

Printing ink is faced various shear stress situation until it transfer to the printed substrate through the press. And in each shear stress condition the ink is needed to keep the appropriate viscosity according to the condition. The change of printing ink viscosity has been explained by well-known through the viscosity profile curve. But actually, the quality of printed paper depends on the ink behavior after transfer the substrates. Like this, to look into the behaviour of the ink on the printed substrate, it is needed the experiment of the ink structure recovering by rheology study. In this study, by controling the $CaCO_3$ content in the ink, after investigating the effect of the ink's structure recovery of the pigment concentration, we intend to predict the printing quality of the ink behvior on the substrate depending on pigment content.

• Reproductive and Developmental Biology
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• v.34 no.4
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• pp.305-308
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• 2010

In this study, we conducted an oral glucose tolerance test (OGTT) so as to compare antidiabetic activities of general potatoes, purple-flesh potatoes, and potato pigments in rats at various concentration levels. After allowing the rats to abstain from food for 12 hours, 10%/20% general potato, purple-flesh potato, and potato extract was orally administered to rats at 100 and 500 mg/kg concentrations. The blood glucose level was measured after an hour. Then, immediately, 1.5 g/kg of sucrose was administered through the abdominal cavity and the blood glucose measured after 30, 60, 120, and 180 minutes. 20% purple-flesh potato group and 10% general potato group, both 100 and 500 mg/kg, showed a significant concentration-dependent decrease in blood glucose levels after 30 minutes. The 100 mg/kg potato pigment group also showed a statistically significant decrease after 30 minutes. In conclusion, administration of 10% general potato, 20% purple-flesh potato, and potato pigment can reduce blood glucose level in an OGTT using rats.

• Journal of Plant Biotechnology
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• v.3 no.1
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• pp.1-5
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• 2001

Traditional culture technology of medicinal plants mainly depends on the field culture, which has many problems. With progress of modern culture technology, it has become possible to produce valuable secondary metabolites from medicinal plants. In this paper, we discuss about the pigment and saikosaponin production from too medicinal plants, Carthamus tinctorius and Bupleurum falcatum, through bioreactor culture system. A two-stage bioreactor culture system was established for the production of yellow and red pigments and saikosaponins by cell suspension cultures of Carthamus tinctorius and Bupleurum falcatum. In Carthamus tinctorius, balloon type airlift bioreactors and column type airlift bioreactors were employed for the tell culture and for the pigment production, respectively. The greatest pigment production was obtained on White medium supplemented with 4 mg/L kinetin, high levels of sucrose concentration and photosynthetic photon flux. In Bupleurum falcatum, adventitious roots were cultured in balloon type airlift bioreactors and the root growth was greatest on SH medium containing 5 mg/L IBA and 0.2 mg/L kinetin. HPLC analysis showed that the contents of main active saikosaponins a, c, and d in adventitious roots were almost the same as those in field cultured root.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.1
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• pp.18-22
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• 2011

Study on extraction and color characteristics of mulberry fruit pigment(C3G; cyanidin-3-glucoside) was performed to increase utilization as new source of natural food colorant. C3G was extracted with 0.1 % citric acid-70% EtOH. Then it was evaporated with large scale evaporation system. After adding dextrin to C3G concentration materials, we made pigment powder with freezing dryer.

• Journal of Life Science
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• v.21 no.4
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• pp.610-612
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• 2011

We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at $50^{\circ}C$ under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-$300\;{\mu}M$). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform.