Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Spectrophotometric Assay for Determination of Chlorogenic Acid Using Green Pigment Formation and Quantitative Analysis of Chlorogenic Acid in Blueberry Leaf

녹색반응을 이용한 클로로겐산의 함량측정을 위한 흡광도 분석법과 블루베리 잎에 함유된 클로로 겐산의 함량분석

  • Chung, Dong-Min (Bioindustrial Process Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Young-Chul (Department of Food Nutrition, International University of Korea) ;
  • Chun, Hyo-Kon (Bioindustrial Process Center, Jeonbuk Branch Institute, Korea Research Institute of Bioscience and Biotechnology)
  • 정동민 (한국생명공학연구원 전북분원 생물산업공정센터) ;
  • 정영철 (한국국제대학교 식품영양학과) ;
  • 전효곤 (한국생명공학연구원 전북분원 생물산업공정센터)
  • Received : 2011.01.06
  • Accepted : 2011.03.01
  • Published : 2011.04.30
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Abstract

We developed a spectrophotometric assay for the quantitative determination of chlorogenic acid based on the formation of green pigment at $50^{\circ}C$ under glycine and alkaline conditions in 96-well plates. The formation of green pigment was linear with a series of chlorogenic acid concentration (0-$300\;{\mu}M$). Using this method, the content of chlorogenic acid (12.42 mg/g dry weight) in the leaves of blueberry was quantified. This method is high-throughput, cost-effective, rapid, and easy to perform.

본 연구는 클로로겐산의 함량을 측정하기 위한 흡광도 방법을 구축하였으며, 구체적으로는 클로로겐산이 $50^{\circ}C$와 글라이신 및 알칼리 조건하에서 녹색반응이 일어난다는 현상을 이용했다. 녹색형성은 일련의 클로로겐산 농도에 의존성을 가졌다. 본 연구에 따른 클로로겐산의 측정방법을 이용하여 블루베리에 함유된 클로로겐산의 함량을 분석하였다(12.42 mg/g d.w). 이러한 방법은 저가의 비용과 빠른 시간으로 많은 시료를 대량으로 쉽게 클로로겐산의 함량은 측정할 수 있는 효과를 가진다.

Keywords

References

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