• Animal Bioscience
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• 제35권10호
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• pp.1592-1605
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• 2022

Objective: The objective of this study was to select an effective in vitro digestion-fermentation model to estimate the effect of decreasing dietary crude protein (CP) on odor emission during pig production and to suggest potential prediction markers through in vitro and in vivo experiments. Methods: In the in vitro experiment, three diet formulations with different CP contents (170 g/kg, 150 g/kg, and 130 g/kg) but containing the same standardized ileal digestible essential amino acids (SID-EAA) were assessed. Each diet was evaluated by two different in vitro gastric-intestinal phase digestion methods (flask and dialysis), combined with fresh pig feces-ferment inoculation. Eighteen growing barrows (31.9±1.6 kg) were divided into three groups: control diet (180 g CP/kg, without SID-EAA adjustment), 170 g CP/kg diet, and 150 g CP/kg diet for 4 weeks. Results: The in vitro digestion results indicated that in vitro digestibility was affected by the gastric-intestinal phase digestion method and dietary CP level. According to the gas kinetic and digestibility results, the dialysis method showed greater distinguishability for dietary CP level adjustment. Nitrogen-related odor compounds (NH₃-N, indole, p-cresol, and skatole) were highly correlated with urease and protease activity. The feeding study indicated that both EAA-adjusted diets resulted in a lower odor emission especially in p-cresol and skatole. Both protease and urease activity in feces were also closely related to odor emissions from nitrogen metabolism compounds. Conclusion: Dialysis digestion in the gastric-intestinal phase followed by fresh fecal inoculation fermentation is suitable for in vitro diet evaluation. The enzyme activity in the fermentation and the fecal samples might provide a simple and effective estimation tool for nitrogen-related odor emission prediction in both in vitro and in vivo experiments.

• Asian-Australasian Journal of Animal Sciences
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• 제20권11호
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• pp.1721-1728
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• 2007

An in vitro and a feeding trial were conducted to investigate the effect of xylanase supplementation on the feeding value of growing pig diets containing high proportions of Chinese double-low rapeseed meals (DLRM). Seven diets were formulated to meet NRC (1998) nutrient requirements. Diet 1 based on corn-soybean meal was used as positive control 1, and diet 2, a practical diet which incorporated a conventional level of Chinese DLRM (60 g/kg diet), as positive control 2. Diet 3 contained a higher level of DLRM (100 g/kg diet) as the negative control. Diet 3 plus xylanase at 0.10, 0.25, 0.50 and 0.70 g/kg diet created diets 4, 5, 6 and 7, respectively. The seven diets were incubated in triplicate with the in vitro two-stage enzyme incubation method to predict responses of diets to xylanase in terms of digestibility of dry matter (DM), crude protein (CP) and neutral detergent fibre (NDF). In vitro, the negative control had the lowest CP and NDF digestibility. Both DM and CP digestibility were increased (p<0.05) owing to xylanase supplementation either at 0.50 or 0.70 g/kg diet, and NDF digestibility was improved following xylanase addition at all of the test levels. There was a high linear correlation ($r^2>90$, p<0.05) between the activity concentration of the enzyme when transformed into its logarithmic value and in vitro digestibility coefficients of DM, CP or NDF. In the feeding trial, 112 crossbred pigs were randomly assigned to seven dietary treatments with 16 replicate pens of one pig each. An obvious dose effect on growth rate was observed ($r^2=0.79$, p<0.05) within the inclusion levels of xylanase. Compared with the negative control, xylanase addition at 0.70 g/kg diet resulted in significantly increased ADG (878 g/d vs. 828 g/d, p<0.05), and a tendency towards improved growth rate (868 g/d vs. 828 g/d, p = 0.10) was also observed following the inclusion of xylanase at 0.50 g/kg diet. It would appear that the nutrient utilization of corn and Chinese DLRM diets by pigs could be enhanced by an appropriate amount of xylanase addition. The in vitro and in vivo results suggested that the in vitro incubation method is feasible for predicting responses of pigs to exogenous enzymes and identifying those preparations that possess potential for improvement of the nutritive values of feedstuffs.

• Asian-Australasian Journal of Animal Sciences
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• 제23권12호
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• pp.1566-1577
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• 2010

Nandrolone, 19-nortestosterone, is a synthetic androgenic-anabolic steroid promoting muscle growth. Nandrolone is also present in pig meat and sera at non-negligible levels. A number of scientific reports have suggested a positive relationship between incidence of infertility and increased meat consumption in humans. The present study was designed to determine out the effect of feeding nandrolone on the testis of the male reproductive tract. Mixtures of food and nandrolone at different concentrations (0.005 ppm and 0.5 ppm) were supplied to pubertal male rats for 6 weeks. Body weight was recorded every week during the entire experimental period. At the end of the treatment, the testis, epididymis, and epididymal fat were collected and weighted. Sperm numbers in the caudal epididymis were counted. Differential gene or protein expression of steroidogenic enzymes in the testes among experimental groups was determined by semi-quantitative real-time PCR or western blotting analysis, respectively. Histological changes of the testis induced by nandrolone treatment were examined by hematoxylin and eosin staining. Immunohistochemical analysis was employed to detect changes in the localization of steroidogenic enzymes in the testes among experimental animals. There were no significant changes on body, testis, epididymis, and epididymal fat weights among experimental groups. A significant increase of caudal sperm number was found in the 0.5 ppm nandrolone-treated group. Histological examination of the testes noted a high frequency of germ cell sloughing in seminiferous tubules of 0.5 ppm nandrolone-treated rats. Even though transcript levels of $3{\beta}$-hydroxysteroid dehydrogenase (HSD) I, $17{\beta}$-HSD4, and $17{\alpha}$-hydroxylase were influenced by nandrolone treatments, protein levels of all molecules examined in the present study were not significantly affected. Immunohistochemical analysis showed no visible changes in the localization of steroidogenic enzymes in the testes among experimental groups. The current study showed that oral intake of nandrolone in male rats for 6 weeks did not cause significant damage to the testis. It is considered that a feeding effect of nandrolone on male fertility would not be remarkable.

• 농업생명과학연구
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• 제45권1호
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• pp.79-88
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• 2011

연구는 사일리지 사료를 급여하였을 때 돼지 품종과 사일리지 급여 유무에 따른 등심의 품질 특성에 미치는 영향을 평가하기 위해 실시하였다. 돼지품종은 가고시마 와타나베 흑돼지 (B), 삼원교잡종[$D(Landrace{\times}Yorkshire){\times} Duroc$], 미국산 버크셔종(A) 등에 대해 각각 시험구와 대조구 20두씩 총120두를 공시하였으며, 개시체중은 평균 $69.3{\pm}3kg$, 시험기간은 평균 $66.7{\pm}9.3$일, 출하일령은 200~210일이었다. 사일리지를 급여하지 않은 대조구 (C)에 비해 급여한 처리구 (T)가 조단백질 함량은 많았고, 육색은 밝았으며, 신선육 관능평가 시 외관, 육색, 상강도 및 전체적 기호도에서 높은 점수를 보였고, stearic산과 oleic산은 낮고, linoleic산, arachidonic산, 필수지방산 및 필수지방산/불포화지방산 비율은 높게 나타났다. 사일리지 급여시 단맛 관련 아미노산 함량은 높은 장점을 지닌 반면 육색의 적색도는 낮고, 가열감량과 육즙감량은 많아 다즙성이 낮았으며, 육의 탄력성이 낮고 지방의 밝기는 어둡고 황색도가 높은 단점을 보였다.

• Journal of Animal Science and Technology
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• 제59권12호
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• pp.28.1-28.8
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• 2017

Background: Major amino acids in pig diets are Lys, Met, Thr, and Trp, but little is known about the requirements for the other essential amino acids, especially on odorous compounds and microbial characteristics in feces of growing-finishing pigs. To this end, different levels of amino acid composition added to diets to investigate the effects of amino acid composition on microbial characteristics and odorous compounds concentration. Methods: A total eight (n = 8) barrows (Landrace ${\times}$ Yorkshire ${\times}$ Duroc) with an average bodyweight of $89.38{\pm}3.3kg$ were individually fed diets formulated by Korean Feeding Standards 2007 (old version) or 2012 (updated with ideal protein concept) in metabolism crates with two replication. After 15-day adaptation period, fresh faecal samples were collected directly from pigs every week for 4 weeks and analysed for total volatile fatty acids (VFA), phenols and indoles by using gas chromatography. The nitrogen was determined by Kjeldahl method. Bacterial communities were detected by using a 454 FLX titanium pyrosequencing system. Results: Level of VFA tended to be greater in 2012 than 2007 group. Among VFAs, 2012 group had greater (p < 0.05) level of short chain fatty acids (SCFA) than control.Concentration of odorous compounds in feces was also affected by amino acid composition in pig diet. Levels of ammonium and indoles tended to be higher in 2012 group when compared with 2007 group.Concentration of phenols, p-cresol, biochemical oxygen demand, and total Kjeldahl nitrogen, however, were lower (P < 0.05) in 2012 treatment group compare to 2007. The proportion of Firmicute phylum were decreased, while the Bacteriodetes phylum proportion increased and bacterial genera includingCoprococcus, Bacillus, and Bacteroides increased (p < 0.05) in 2012 compare to 2007 group. Conclusion: Results from our current study indicates that well balanced amino acid composition reduces odor by modulating the gut microbial community. Administration of pig diet formulated with the ideal protein concept may help improve gut fermentation as well as reduce the odor causing compounds in pig manure.

• 한국환경농학회지
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• 제20권2호
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• pp.122-126
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• 2001

MS균을 이용하여 고온숙성에 의해 남은 음식물을 양돈사료로 제조하여 양돈 배합사료의 대체효과 및 사료효율에 미치는 영향을 조사하기 위하여 배합사료 100% 급여 (대조구), 남은 음식물 발효사료 대체율을 배합사료 대비 25%, 50% 및 100% 대체 급여구, 4처리로 설정하여 5반복으로 사양시험을 실시하였다. 남은 음식물 발효사료의 전 시험기간 동안 (10 주령부터 27주령까지) 일당 중체량은 배합사료 100% 급여한 대조구에 비해 남은 음식물 발효사료를 25% 및 50% 대체 했을 때 29.4% 및 13.9%로 높았고, 100% 대체구는 38.2% 감소하였다. 일일사료 섭취량, 사료요구율에 있어서 남은 음식물 발효사료를 50% 대체구에서는 대조구에 비해 다소 떨어지지만 유의적인 차이는 보이지 않아 배합사료를 남은 음식물 발효사료로 대체할 수 있는 수준인 것으로 나타났다. 그러나 100% 대체구에서는 대조구에 비해 유의적으로 차이가 있어 돼지 성장에 문제가 있는 것으로 평가되었다. 1 kg당 증체에 요구되는 사료비는 배합사료만 급여한 대조구에서 690.4원인데 비하여 25% 및 50% 남은 음식물 발효사료 대체구에서는 총 증체량이 대조구보다 높게 유지되면서 28.6% 및 49.4%가 절감되는 높은 경제성을 확인할 수 있었다. 100% 남은 음식물 발효사료 급여구에서는 kg 증체에 요구되는 사료비는 약 32.6% 감소되었으나, 전 육성기간동안의 총 증체량이 26.5 kg으로 시험 종료시 출하체중에 훨씬 못 미쳐 실제사양에 적용 가능성은 매우 어려운 것으로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제16권7호
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• pp.1025-1029
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• 2003

The effect studied was that of the feeding of shrimp by-product meal, as a source of eicosapentaenoic and docosahexaenoic acid, on growth performance and fatty acid composition of adipose tissue in growing pigs kept on small holdings in Central Vietnam. Shrimp by-product meal was exchanged with ruminant meal so that the diets contained either 0, 10 or 20% shrimp byproduct meal in the dry matter. The diets were fed on 6 different small-holder farms. The farmers fed a base diet according to their personal choice, but were instructed as to the use of shrimp by-product and ruminant meal. The diets were fed to the pigs from 70 to 126 days of age. There were three animals per treatment group per farm. The diets without and with 20% shrimp by-product meal on average contained 0.01 and 0.14 g docosahexaenoic acid/MJ of metabolisable energy (ME). Due to the higher contents of ash and crude fiber, the shrimp by-product meal containing diets had lower energy densities than the control diets. Eicosapentaenoic acid was not detectable in adipose tissue; the content of docosahexaenoic acid was generally increased after consumption of shrimp by-product meal. In spite of the concurrent high intakes of ash and crude fiber, the feeding of shrimp by-product meal had a general stimulatory effect on growth performance of the growing pigs. The intake of docosahexaenoic acid or its content in adipose tissue was not related with average daily gain. It is suggested that shrimp by-product meal may contain an unknown growth enhancing factor.

• Asian-Australasian Journal of Animal Sciences
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• 제14권10호
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• pp.1444-1449
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• 2001

Two feeding trials were conducted to determine the effect of inclusion levels or deletion of vitamin-trace mineral (VM) premixes on growth performance and pork quality in finishing pigs. In exp. 1, a total of ninety-six crossbred pigs $(Landrace\;{\times}\;Yorkshire\;{\times}\;Duroc,\;85.09{\pm}3.12kg)$ were used for a 4-week feeding triaL Treatments were premix supplementation at the level of 50% (Control), 100%, 150%, and 200% of NRC (1998) requirements for vitamins and trace minerals. In exp. 2, a total of one hundred and eight crossbred pigs $(Landrace\;{\times}\;Yorkshire\;{\times}\;Duroc,\;84.76{\pm}0.58kg)$ were used for a 4-week feeding trial. Treatments were premix supplementation at the level of 0% (Control), 200% VM, and 200% vitamin E and Se listed in NRC (1998) requirements. Average daily gain (ADG) and feed/gain (F/G) were the highest at 150% VM addition level (quadratic, p<0.05) among treatments. Dressing percentage and backfat thickness in pigs were not affected by different addition levels of VM premixes. Pork stability in terms of TBARS was linearly (p<0.05) improved as dietary VM premix was increased (exp. 1). ADG, F/G and pork stability (TBARS) were also reduced (p<0.05) when VM premixes were deleted. However, supplementation of vitamin E and Se improved (p<0.05) ADG and pork stability when pigs were fed diets without VM premixes (exp. 2). In conclusion, deleting dietary VM premixes gave negative effects on growth performance and pork quality for the last 4 weeks of finishing period.

• Asian-Australasian Journal of Animal Sciences
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• 제13권9호
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• pp.1304-1308
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• 2000

A total of thirty-six gilts $(24.78{\pm}1.39kg)$ were employed for 38 days (Exp. 1), and twenty-four gilts $(46.50{\pm}1.90kg)$ for 43 days (Exp. 2) to determine growth performance and carcass characteristics in pigs fed dried food waste (DFW). Pigs were allocated by dietary treatments: 0%, 20% and 40% DFW. For nutrient digestibility, twelve female pigs $(50.20{\pm}0.52kg)$ were used in individual pens to collect feces. Food wastes were collected from restaurants and apartment complex areas and dried in a drum-type dryer at $115{\pm}2{^{\circ}C}$. Experimental diets for feeding trials were formulated to contain 3,400 kcal DE/kg and 17% crude protein. The tested DFW contained 2,858 kcal DE/kg, 25.0% crude protein, 17.3% crude fat, 1.37% Ca, 1.28% P, and 3.28% NaCl. As compared to crude protein content, the limiting amino acids (i.e., 0.90% lysine and 0.52% methionine) were low. The digestibilities of energy, ash, calcium and phosphorus in the DFW were generally lower than those in the grower diets, but the digestibilities of crude protein and crude fat were higher in DFW than the grower diet. Feeding DFW in pigs had a linear (p<0.01) effect on ADG and feed/gain as the inclusion levels of DFW were increased (Exp. 1). The ADG of pigs fed 40% DFW was also poorer (p<0.05) than that fed the control diet (Exp. 2). Carcass characteristics in terms of backfat and dressing percentage were not affected by dietary treatments. In conclusion, it seems that the optimal dietary inclusion level of the DFW is about 20% in the diet for growing-finishing pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1615-1634
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• 2004

A review was undertaken to obtain information on the sustainability of pig free-range production systems including the management, performance and health of pigs in the system. Modern outdoor rearing systems requires simple portable and flexible housing with low cost fencing. Local pig breeds and outdoor-adapted breeds for certain environment are generally more suitable for free-range systems. Free-range farms should be located in a low rainfall area and paddocks should be relatively flat, with light topsoil overlying free-draining subsoil with the absence of sharp stones that can cause foot damage. Huts or shelters are crucial for protecting pigs from direct sun burn and heat stress, especially when shade from trees and other facilities is not available. Pigs commonly graze on strip pastures and are rotated between paddocks. The zones of thermal comfort for the sow and piglet differ markedly; between 12-22$^{\circ}C$ for the sow and 30-37$^{\circ}C$ for piglets. Offering wallows for free-range pigs meets their behavioural requirements, and also overcomes the effects of high ambient temperatures on feed intake. Pigs can increase their evaporative heat loss via an increase in the proportion of wet skin by using a wallow, or through water drips and spray. Mud from wallows can also coat the skin of pigs, preventing sunburn. Under grazing conditions, it is difficult to control the fibre intake of pigs although a high energy, low fibre diet can be used. In some countries outdoor sows are fitted with nose rings to prevent them from uprooting the grass. This reduces nutrient leaching of the land due to less rooting. In general, free-range pigs have a higher mortality compared to intensively housed pigs. Many factors can contribute to the death of the piglet including crushing, disease, heat stress and poor nutrition. With successful management, free-range pigs can have similar production to door pigs, although the growth rate of the litters is affected by season. Piglets grow quicker indoors during the cold season compared to outdoor systems. Pigs reared outdoors show calmer behaviour. Aggressive interactions during feeding are lower compared to indoor pigs while outdoor sows are more active than indoor sows. Outdoor pigs have a higher parasite burden, which increases the nutrient requirement for maintenance and reduces their feed utilization efficiency. Parasite infections in free-range pigs also risks the image of free-range pork as a clean and safe product. Diseases can be controlled to a certain degree by grazing management. Frequent rotation is required although most farmers are keeping their pigs for a longer period before rotating. The concept of using pasture species to minimise nematode infections in grazing pigs looks promising. Plants that can be grown locally and used as part of the normal feeding regime are most likely to be acceptable to farmers, particularly organic farmers. However, one of the key concerns from the public for free-range pig production system is the impact on the environment. In the past, the pigs were held in the same paddock at a high stocking rate, which resulted in damage to the vegetation, nutrient loading in the soil, nitrate leaching and gas emission. To avoid this, outdoor pigs should be integrated in the cropping pasture system, the stock should be mobile and stocking rate related to the amount of feed given to the animals.