• 동의생리병리학회지
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• 제22권4호
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• pp.802-809
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• 2008

Gamijangsing-tang (GMJST) has been used for treatment of bone formation in traditional korean medicine. The purpose of this study is to examine effects of GMJST on bone metabolism. The effects on the osteoblasts were determined by measuring (1) cell proliferation, (2) alkaline phosphatase (ALP) activity, (3) osteoprotegerin (OPG) secretion. (4) The morphologic changes of cells were observed by light microscopy and electron microscopy. Mineralization of calcium was determined by quantitative alizarin red-S assay and mineralization of phosphate was observed by von kossa staining. The morphologic changes of mineralization on the cells were observed by transmission electron microscopy (TEM). The effects on the osteoclast were investigated by tartrate-resistant acid phosphatase (TRAP) staining. Following results were obtained: Celluar activity of osteoblastic cells (MG-63) was significantly increased in 10-5 of dilution of GMJST. ALP and OPG activity of osteoblastic cells were increased in GMJST than normal MG-63 cell. Mineralization of osteoblastic cells were increased in GMJST than normal MG-63 cell. The activity of osteoclast cells (RAW 264.7) was significantly decreased in GMJST than normal MG-63 cell. From the results, GMJST stimulated the proliferation and mineralization of bone-forming osteoblast and inhibited by bone- lysis osteoclast.

• 동의생리병리학회지
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• 제32권3호
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• pp.171-177
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• 2018

The purpose of this study was to evaluate the reliability and the validity of Deficiency and Excess Pattern Identification Questionnaire. The number of subjects enrolled in this study was 248, from July 2015 to March. 2016. The surveys was conducted two times with 3 month interval. The Cronbach's ${\alpha}$ analysis for internal reliability, Pearson Correlation Coefficient analysis for test-retest reliability were conducted. Factor analysis with varimax rotation for construct validity was used. Kappa analysis for diagnostic reliability were used. The significant p-value was < .05. The Cronbach's ${\alpha}$ was .929 in Deficiency and .932 in Excess questionnaire. The reliabilities between test and retest Intra Correlation Coefficient (ICC) was .71-.762 in the Deficiency, and .58-.786 in Excess questionnaire, respectively. Deficiency was divided by five factors, and Excess four factors. The factor convergence was 72.54% in the Deficiency and 67.5% in Excess questionnaire. The test-retest agreement of four pattern was 68.5% and Kappa was .530. This study reveals that Deficiency and Excess Pattern Identification Questionnaire is a reliable and valid. However, further study to validate the questionnaire is needed.

• The Journal of Korean Physical Therapy
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• 제26권5호
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• pp.351-357
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• 2014

Purpose: The purpose of this study was to investigate level of functioning in patients with stroke using Modified Bathel Index (MBI), World Health Organization Disability Assessment Schedule 2.0 (WHODAS 2.0), and ICF core-set for stroke. Methods: Sixty-four patients with stroke were recruited for this study from nine medical institutes. The ICF core-set for stroke, WHODAS 2.0, and MBI were used to collect subjects' functional levels. ICF core-set was employed here as a standard frame to observe multi-dimension of functioning, that is physiological bodily function, activity and participation (AP) in daily life, and current environmental factors (EF) in patients with stroke. WHODAS 2.0 and MBI were also used in order to have a specific functioning level for subjects. The linkage of each item in WHODAS 2.0 and MBI into the ICF core-set for stroke was examined. Pearson correlation coefficient was used for analysis of their relationships. Results: Functioning level of participants showed moderate resulting from MBI and WHODAS 2.0 ($73.48{\pm}22.27$ and $35.55{\pm}12.53$, respectively). Strong relationship was observed between ICF core-set and WHODAS 2.0, and with MBI. Each item of disability scales was obtained its linkage into ICF in the domain of AP. However, lack of correlation between MBI and ICF in the domain of EF was found due to absence of related factors. Conclusion: MBI was found to be linked mainly into ICF in the domain of AP and to have limited linkage into EF. Therefore, it should be suggested that the ICF concept frame should be used as a multi-dimensional approach to patients with stroke.

• 동의생리병리학회지
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• 제28권3호
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• pp.288-295
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• 2014

Bojungchiseub-tang (BJCST) has been used in symptoms and signs of edema, dampness-phlegm, kidney failure, and so on. BJCST is also expected to have strong anti-obesity activities. However, little is known about the mechanisms of its inhibitory effects on adipocyte differentiation and adipogenesis. In the present study, we examined the effects and mechanism of BJCST on transcription factors and adipogenic genes of 3T3-L1 preadipocytes to understand its inhibitory effects on adipocyte differentiation and adipogenesis. Our results showed that BJCST significantly inhibited differentiation and adipogenesis of 3T3-L1 preadipocytes in a dose-dependent manner. To elucidate the mechanism of the effects of BJCST on lowering lipid content in 3T3-L1 adipocytes, we examined whether BJCST modulate the expressions of transcription factors to induce adipogenesis and adipogenic genes related to regulate accumulation of lipids. As a result, the expression of steroid regulatory element-binding protein (SREBP)1, cytidine-cytidine-adenosine-adenosine-thymidine (CCAAT)/enhancer binding proteins ${\alpha}$ ($C/EBP{\alpha}$), $C/EBP{\beta}$, $C/EBP{\delta}$, and peroxisome proliferator-activated receptor ${\gamma}$ ($PPAR{\gamma}$) genes, which induce the adipose differentiation, liver X receptor $(LXR){\alpha}$ and fatty acid synthase (FAS) genes, which induce lipogenesis and adipose-specific aP2, Adipsin, lipoprotein lipase (LPL), CD36, TGF-${\beta}$, leptin and adiponectin genes, which compose fat formation were decreased. BJCST also reduced the expression of acyl CoA oxidase (ACO) and uncoupling protein (UCP) genes related to lipid oxidation. In conclusion, BJCST could regulate transcript factor related to induction of adipose differentiation and inhibited the accumulation of lipids and expression of adipogenic genes.

• 동의생리병리학회지
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• 제28권3호
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• pp.346-354
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• 2014

The study was performed to determine the reliability and validity of the 'Qi Blood Yin Yang Deficiency Questionnaire (Qi Blood Yin Yang DQ)' for the 100 subjects with chronic fatigue. After 100 subjects respond to the 'Qi Blood Yin Yang DQ', Korean medical doctor classified the subjects into 4 groups such as Qi deficiency group, Blood deficiency group, Yin deficiency group and Yang deficiency group. 100 subjects were retested in the same way after 3 weeks. The reliability and the validity of the questionnaire were inspected. Internal consistency of the 'Qi Blood Yin Yang DQ' was excellent (Cronbach alpha 0.916). Test-retest reliability was good (intra-class correlation coefficient 0.699). Construct validity analyzed by exploratory factor analysis produced 4 factors, which were selected from eigenvalues that are greater than 1.0. The 'Qi Blood Yin Yang DQ' consisted of 4 factors. The factor 1, 2, 3 and 4 showed 'Qi deficiency', 'Yin deficiency', 'Yang deficiency' and 'Blood deficiency' respectively. The 'Qi Blood Yin Yang DQ' restructured in this study may provide a fundamental questionnaire and a further study is required for a more advanced, standardized and statistically proven questionnaire.

• Journal of Trauma and Injury
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• 제32권4호
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• pp.220-225
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• 2019

Purpose: Mechanical ventilation (MV) is an essential life-saving modality for severely injured patients. However, the long-term use of MV is a major risk factor for late mortality. The surgical correction of long bone fractures plays a critical role not only in improving functional outcomes, but also in reducing physiological derangements, including MV duration. This study investigated the factors affecting prolonged MV (PMV) in severely injured patients with femur fractures. Methods: We retrospectively evaluated all severely injured patients (injury severity score >15) with femur fractures who were taken to the emergency department within 12 hours of the causative accidents between January 2016 and December 2018. PMV was defined as MV lasting for ≥7 days. We analyzed the factors affecting PMV. Results: In total, 35 patients were enrolled and 21 (33.3%) were included in the PMV group. The PMV group required more red blood cell (RBC) transfusions within 7 days RBC (7dRBC) (12.8 vs. 6.8 units; p=0.03) and the time to femur fracture fixation (TFFF) was longer (7.9 vs. 2.7 days; p=0.018). The area under the curve (AUC) for TFFF was 0.740 (95% confidence interval [CI]: 0.572-0.908; p=0.018) and the AUC for 7dRBC was 0.718 (95% CI: 0.546-0.889; p=0.031). Conclusions: This study indicates that TFFF is an independent risk factor for PMV. Early fixation of femur fractures might prevent PMV and its associated complications.

• Asian-Australasian Journal of Animal Sciences
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• 제23권8호
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• pp.1118-1126
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• 2010

Male reproduction is influenced by a number of intrinsic and extrinsic factors, including environmental endocrine disruptors. Testosterone is a well recognized intrinsic regulator for development and function of the male reproductive tract, and thus male fertility. The testis and semen of many mammalians contain an unusually high concentration of estrogen. Testosterone is converted into estrogen by the enzymatic action of cytochrome P450 aromatase complex (Cyp19a1). Of the male reproductive tract, the efferent ductules (EDs) possess exceptionally elevated levels of estrogen receptors (ERs), ER${\alpha}$ and ER${\beta}$, indicating that estrogen, in addition to testosterone, would have a functional role in regulation of male reproduction. First, this review has focused on description and summary of what is currently known for functions of estrogen in the EDs. The biosynthetic pathway of estrogen occurring in the testis is briefly covered, following by detailed explanation of the morphology and physiology of EDs. In the next section, the sources and targets of estrogen in the male reproductive tract are highlighted, and possible functional roles of estrogen in the EDs are justified from the aspect of physiology, molecular biology, and morphology in adult animal models. Also, this section covers the importance of estrogen and ERs in maintaining normal function and morphology of the EDs during postnatal development. In the last part of this review, the effects of extrinsic factors, especially environmental endocrine-disruptors, on the EDs is summarized. The intent of this review is to emphasize the importance of estrogen for regulation of physiological function of the EDs, and thus male fertility.

• 여성건강간호학회지
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• 제3권1호
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• pp.51-71
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• 1997

A Pregnancy is the women's normal physiological and developmental process, in which many psychological changes including sexual problems always occur. But there have been few guidelines on sexual life during pregnancy. So this study was performed to evaluate the sexual life of pregnant women, and to analyze the factors affecting satisfaction of sexual life during pregnancy. Questionnaires were given to pregnant women who visited two general hospitals for antenatal care as well as to attendants at a prenatal Lamaze class. The results were as followings : 1) The average age of the 194 pregnant women was 28.5 years ; 98.4% of them had educated more than 12 years. ; the average monthly income was about 15,000,000 won ; primigravida women were 81.4% of the total cases. 2) As the pregnancy progressed, the frequency of the sexual intercourses was decreased in 48.5% because of the anxiety on the fetal damages. The was no significant differences between frequency of sexual intercourses and the degrees of physical discomfort and spouse intimacy. Man superior position was taken in 88.1% ; the incidence of orgasms before and during pregnancy were 71.1% and 41.1%, respectively ; the incidence of painful sexual intercourse in primigravida women was decreased by pregnancy in 4.6%, and in the case of multigravida women it was increased in 2.1%. 3) The average point of sexual knowledge was 11.5 in total points of 20 ; that of sexual attitude was 29.1 in total points of 40 ; that of sexual satisfaction was 28.5 in perfects points of 40. 4) The factors which significantly related to the satisfaction of sexual life were spouse intimacy, sexual attitude, change of sexual body position during pregnancy, and the orgasms before and during pregnancy. Considering above results, nursing care and nursing intervention including sexual education and counselling programs are needed during pregnancy as well as before pregnancy.

• 부모자녀건강학회지
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• 제5권2호
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• pp.161-176
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• 2002

Among the causes of childhood behavioral problems - that is, physiological, psychological, and environmental factors -, the common element of non-organic psychological and environmental factors is influences from the parents. Roles of mother who does the biggest emotional exchange with the child after birth may have substantial association with the childhood behavioral problems. In this connection, the present explanatory study was conducted for the purpose of understanding the causal structural relationship in which mother's child caring behaviors affect child behavioral problems. The data collection period was July 2 - 7, 2001. The subject was 4th, 5th, 6th graders' mothers in 4 elementary schools located in Daejoen city and 299 surveys were used in the analysis. As for the tools used in this study to assess the mother's child caring Behavior Scale by Park, Seong-Yeon and Yi, Sook (1990) was used to measure the mother's behaviors. The child behavioral problems were measured with the Korean-Child Behavior Checklist (K-CBCL), standardized into the Korean version by O, Gyung-Ja, Yi, Hye-Ryon, Hong, Gang-Eui, and Ha, Eun-Hye. For the data processing, the frequency and the percentage were calculated, and the analyses of variance, correlation were carried out. The result of this study is summarized as follows: First, the examination of the effect of mother's general characteristics on the mother's caring behavior shows that significant differences are made by the active participation (p=0.020) in the occupation, affection(p=0.000), authority control(p=0.013), achievement(p=0.030), active participation(p=0.032) in the education level, consistency regulation(p=0.007) in the religion. Second, the examination of the effect of mother's caring behaviors on the child behavioral problems shows that significant differences are made by some child behavioral problems in the affection, achievement, consistency regulation, authority control, over protection caring behaviors. Third, as for the correlation between mother's child caring behavior and child behavioral problems, a significant correlation is revealed between the negative child caring behavior and child behavioral problems.

• Journal of Plant Biotechnology
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• 제36권2호
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• pp.124-129
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• 2009

In general, the root color of Codonopsis lanceolata is white, but red or blue-colored root is found at a low frequency in nature. Red or blue-colored roots have scarcity value, thus farmers wish to produce colored roots. The factors for determining the color of roots are unclear whether the color is controlled by genetically or simply by environmentally such as soil environment. Using in vitro culture system which is advantageous for setting of the same culture condition, we analyzed the physiological and morphological characteristics and genetic differences among red-, blue- and white lines of C. lanceolata. In the red colored roots, stems of in vitro cultured plantlet were colored in dark red pigment. Histological analysis revealed that the red pigment was accumulated in the outer cortex layer of the stem and determined as anthocyanin. Chlorophyll contents in red root lines were higher than those in white- and blue root lines. Plantlets from red roots were smaller in both shoot length and total leaf area than those from white- and blue roots. Genetic differences among the three different colored C. lanceolata were determined by RAPD (Randomly Amplified Polymorphic DNA) analysis. Each line of colored roots had clear DNA polymorphism. These results indicate that the occurrence of red- and blue colored roots in nature was determined by genetic factors rather than soil enviromental conditions.