• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.383-384
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• 2000

Most of the photoperiodic responses result in a sharp contrast between development in long and short days, so that qualitative observations are quite sufficient to establish that development is under photoperiodic control, and qualitative information of this type is all that is available for many of the responses (Dring 1984). However, we need to devise some quantitative measurement of the response in order to study the physiological mechanism of photoperiodic control. We report here the quantification of photoperiodic control of erect thalli production of Sargassum muticum in different daylength regimes. (omitted)

• Journal of Plant Biology
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• v.19 no.1
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• pp.14-18
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• 1976

Inhibition of flowering in Lemna perpusilla 6746 by 30 mM sucrose was reversed by the addition of acetylcholine (>$10^{-4}M) supplemented with 10^{-4}M$ ascorbic acid to 1/10-strength Hunter's growth medium. The reversible effect of acetylcholine was found to be greater at early stages of flowering than in the later period. Promotive effects of both acetylcholine ($10^{-3}M) and eserine(10^{-5}M$) on flowering in the short-day plant under various photoperiodic conditions were studied. It was indicated that the application decreased length of the critical dark period for the floral induction, and it was also shown that the endogenous status of acetylcholine was involved in the floral response which had a correlation with phytochrome. Interruption of inductive dark periods by red irradiation (1min) immediately followed by far-red light (1 min) completely inhibited flowering, while the addition of acetylcholine and eserine to the medium under the same condition slightly promoted flowering, indicating possible involvement of phytochrome system in acetylcholine activity for photoperiodic sensitivity of floral response in Lemna perpusilla 6746.

• Journal of Microbiology and Biotechnology
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• v.29 no.9
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• pp.1453-1459
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• 2019

Zeaxanthin is an important pigment in the photo-protection mechanism of microalgae. However, zeaxanthin epoxidase, an enzyme involved in the accumulation and conversion of zeaxanthin, has not been extensively studied in microalgae. In this work, we report the expression pattern of zeaxanthin epoxidase in Dunaliella tertiolecta (DtZEP) at different light and diverse salinity conditions. To confirm the responsiveness to light conditions, the ZEP expression pattern was investigated in photoperiodic (16 h of light and 8 h of dark) and continuous (24 h of light and 0 h of dark) light conditions. mRNA expression levels in photoperiodic conditions fluctuated along with the light/dark cycle, whereas those in continuous light remained unchanged. In varying salinity conditions, the highest mRNA and protein levels were detected in cells cultured in 1.5 M NaCl, and ZEP expression levels in cells shifted from 0.6 M NaCl to 1.5 M NaCl increased gradually. These results show that mRNA expression of DtZEP responds rapidly to the light/dark cycle or increased salinity, whereas changes in protein synthesis do not occur within a short period. Taken together, we show that DtZEP gene expression responds rapidly to light irradiation and hyperosmotic stress. In addition, ZEP expression patterns in light or salinity conditions are similar to those of higher plants, even though the habitat of D. tertiolecta is different.

• Development and Reproduction
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• v.19 no.1
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• pp.11-17
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• 2015

This study investigated possible involvement of photoperiodic regulation in reproductive endocrine system of female olive flounder. To investigate the influence on brain-pituitary axis in endocrine system by regulating photoperiod, compared expression level of Kisspeptin and sbGnRH mRNA in brain and FSH-${\beta}$, LH-${\beta}$ and GH mRNA in pituitary before and after spawning. Photoperiod was treated natural photoperiod and long photoperiod (15L:9D) conditions from Aug. 2013 to Jun. 2014. Continuous long photoperiod treatment from Aug. (post-spawning phase) was inhibited gonadal development of female olive flounder. In natural photoperiod group, the Kiss2 expression level a significant declined in Mar. (spawning period). And also, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels were increasing at this period. However, in long photoperiod group, hypothalamic Kiss2, FSH-${\beta}$, LH-${\beta}$ and GH mRNA expression levels did not show any significant fluctuation. These results suggest that expression of hypothalamic Kiss2, GtH and GH in the pituitary would change in response to photoperiod and their possible involvement of photoperiodic regulation in reproductive endocrine system of the BPG axis.

• Fisheries and Aquatic Sciences
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• v.22 no.1
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• pp.4.1-4.10
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• 2019

Russian sturgeon (Acipenser gueldenstaedtii) is commercially important in Korea because its caviar is highly prized. Although the early ontogeny of the species has been described, behavioral modifications in response to various light intensities or diel photoperiodicity patterns have not been studied extensively. The objective of the present study was to examine the behavioral characteristics of hatchery-produced A. gueldenstaedtii prelarvae over a diel photoperiodic cycle. During a diel light cycle comprising 16 h of daylight (450 lx), 4 h of dim light (10 lx), and 4 h of darkness (< 1 lx), newly hatched A. gueldenstaedtii prelarvae exhibited negative phototaxis in daylight (day 0-day 2), and this early behavior was not significantly affected by changes of light intensities. Rheotactic and non-rheotactic aggregation into schools was typical between days 3 and 6. Under the diel light cycle conditions described, rheotaxis was not influenced by environmental light intensity as much as non-rheotactic schooling behavior. Post-schooling behavior, which progressed from day 7 to day 8, did not change significantly during the diel light cycle. The data from the present study could be of practical value in developing a visual guide for assessing the fitness and quality of Russian sturgeon prelarvae under diel light cycle conditions in hatcheries.

• Journal of Korean Society of Forest Science
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• v.88 no.4
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• pp.523-532
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• 1999

The studies were carried out to develop an urban forest aromatic bath in the idle space within city area for handicapped persons, patients and city workers, who having less chance to visit the natural forest aromatic bath. The environmental conditions were checked in the experimental double vinyl house where native and exotic aromatic woody and herbaceous plants were growing with minimum energy input in the periods of mid-winter and mid-summer. The growth and photoperiodic responses of the plants to the low and high temperatures were observed during winter and summer respectively. The most of herbaceous plants in the double vinyl house with the controlled minimum night temperature of $-2^{\circ}C$ had no winter injury but did not grow and started the growth in April. The exotic herbs such as Catnip faassen's(Nepeta x faassenia) and Lemon bergamot(Monarda citriodora) grew better in the longer photoperiodic conditions. The growth rates of Abies koreana, Thuja koraiensis and Pinus sylvestriformis in the early spring were affected mainly by the night minimum temperature and less affected by the photoperiodic conditions during winter. The native aromatic herbs such as Robdosia japonica, Molsa punctulata, Agastache rugosa and Codonopsis lanceolata showed stimulatory growth at the 16 hours day-length in case of the high temperature vinyl hose. The height and root collar diameter growth of pine seedlings were inhibited in maxim temperatures over $40^{\circ}C$ during summer.

• BMB Reports
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• v.51 no.4
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• pp.163-164
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• 2018

CONSTANS (CO) induces the expression of FLOWERING LOCUS T (FT) in the photoperiodic pathway, and thereby regulates the seasonal timing of flowering. CO expression is induced and CO protein is stabilized by FLAVIN-BINDING KELCH REPEAT F-BOX PROTEIN 1 (FKF1) in the late afternoon, while CO is degraded by CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1) during the night. These regulatory cascades were thought to act independently. In our study, we investigated the relationship between FKF1 and COP1 in the regulation of CO stability in response to ambient light conditions. A genetic analysis revealed that FKF1 acts as a direct upstream negative regulator of COP1, in which cop1 mutation is epistatic to fkf1 mutation in the photoperiodic regulation of flowering. COP1 activity requires the formation of a hetero-tetramer with SUPPRESSOR OF PHYA-105 (SPA1), [$(COP1)_2(SPA1)_2$]. Light-activated FKF1 has an increased binding capacity for COP1, forming a FKF1-COP1 hetero-dimer, and inhibiting COP1 homo-dimerization at its coiled-coil (CC) domain. Mutations in the CC domain result in poor COP1 dimerization and misregulation of photoperiodic floral induction. We propose that FKF1 represses COP1 activity by inhibiting COP1 dimerization in the late afternoon under long-day conditions, resulting in early flowering.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.8 no.1
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• pp.111-115
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• 1970

1. Eight Varieties of Nicotiana tabacum, "Yellow special A", "Gold leaf", "Oxford, " "Awha", "Yungzung", "Samsun", "Maruha" and "Hatano" were compared on the photoperiodic responses. 2. Experiments were conducted both under high temp. condition(green-house, average temp $25^{\circ}C$-$35^{\circ}C$) and low temp. condition (out-door; average temp $10^{\circ}C$-$25^{\circ}C$) in four plots of 8.10.12 and 14 hours in day length. 3. The results obtained are summarized as follows; i) In the short day condition, the flowering was accelerated in the low temp. than high. On the contrary, in the high temp, it was accelerated in the long day. ii) Generally speaking, the flowering was responsed more in low temp. and short day length than high temp.-long day. iii) But the above trend was found great difference among varieties..-long day. iii) But the above trend was found great difference among varieties.

• Proceedings of the Korean Society of Crop Science Conference
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• 2000.04a
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• pp.410-411
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• 2000

• Proceedings of the Korean Society of Life Science Conference
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• 2001.02a
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• pp.83-83
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• 2001