• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.5
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• pp.900-907
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• 2000

The antioxidant effects of dichloromethane, ethylacetate or water fraction of kimchi added to the 1% cholesterol diet were studied. Six New Zealand white rabbits in each group were fed either control diet (basal diet containing 1% cholesterol) or experimental diet containing dichloromethane (CH$_2$Cl$_2$), ethylacetate (EtOAc) or water ($H_2O$) fraction of kimchi in the control diet for 16 weeks. The amount of each solvent fraction of kimchi added to the experimental diet was equivalent to 5% of freeze-dried kimchi. Levels of hepatic lipid oxidation expressed as TBARS or peroxide value for the experimental groups were lower than that of control (p<0.05). Liver homogenated of the experimental group containing dichloromethane fraction of kimchi inhibited LDL oxidation in the presence of Cu++ by 46% (p<0.05). The activities of catalase, Glutathione peroxidase (GSH-Px), Cu, Zn-superoxide (Cu, Zn-SOD) and Mn-superoxide (Mn-SOD) of experimental groups were lower than those of control group. Low enzyme activities observed from the kimchi solvent fraction groups might be due to the level of lipid oxidation progressed less in these groups. The most significant antioxidant effects were observed from dichloromethane fraction of kimchi among the experimental groups. The major fatty acids of hepatic phospholipid of rabbit were C18:2 and C18:0. But the major fatty acid profile was changed into C16:0, C18:0, C18:1, and C18:2 when rabbit was fed 1% cholesterol diet for 16 weeks, and this profile was almost the same as in rabbit fed diet containing kimchi solvent fraction. The ratio for unsaturated fatty acid to saturated fatty acid decreased by cholesterol induced diet and it was not corrected by kimchi solvent fractions.

• Korean Journal of Food Science and Technology
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• v.12 no.4
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• pp.278-284
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• 1980

Lipids from rice bran (Indica type Milyang #23), both fresh and stored at $30^{\circ}C$ and 80% relative humidity for 5 weeks, were separated and analyzed for the determination and the storage effect on the bran lipid composition. Total lipids of fresh rice bran consisted of 89.9% neutral lipids, 8.0% glycolipids, 2.1% phospholipids and no significant changes of these fractions were noted during storage. Triglycerides(43.1%), diglycerides(13.8%) and hydrocarbon-esterified sterol(13.5%) among six fractions were considered as main components in neutral lipids. After storage triglycerides content significantly decreased as the free fatty acid increased in the neutral lipid fraction. Major components of the glycolipid fraction were acylsterolglycoside(43.1%) and sterolglycoside(30.3%). Phosphatidyl choline(39.8%), phosphatidyl serine(20.9%) and phosphatidyl ethanolamine(19.8%) were predominent in the phospholipid fraction. No significant changes of the composition were shown in fraction of the glycolipid or the phospholipid during the storage period. Major fatty acids of the total lipid fraction were oleic(44.3%), linoleic(32.5%) and palmitic acids(18.4%). The fatty acid compositions of the neutral lipid, the glycolipid and the phospholipid fractions were similar to the total lipid fraction. Small changes in fatty acid composition in each fraction were noted during the storage period. The acid value increased but iodine value decreased during the storage period. The values of peroxide and TBA increased gradually in the first three weeks, and then slowly decreased in the fourth and the fifth week of the storage.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.5
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• pp.418-425
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• 1991

This study was designed to observe the effects of the fish oil and some seed oils on the improvement of the fatty acid compositions of liver and brain tissue in rats. In order to induce the hypertriglyceridemia in the rats of the Sprague-Dawley, 12% coconut oil and 3% each of olive oil, lard, fish oil, perilla oil, corn oil, red pepper seed oil and evening primrose oil were administered to the rats for 4 weeks. In the fatty acid composition of liver lipid, n-3 PUFA contents were most in the fish oil and perilla oil groups of phospholipid fraction, and n-6 PUFA contents were most in the corn oil, red pepper seed oil and evening primrose oil groups of triglyceride fractions. Fatty acid composition of liver lipid fractions were influenced from the fatty acid composition of the test lipids. In the fatty acid composition of brain phospholipid, n-3 PUFA contents (8.8~17.2%) were most in the fish oil group, and n-6 PUFA (34.6~38.2%), though it contains high percentage, showed little difference between groups.

• Analytical Science and Technology
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• v.13 no.5
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• pp.592-600
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• 2000

The concentration of free fatty acids and fatty acid composition as well as cholesterol supersaturation in bile may be an important factor in the gallstone formation. Therefore, we simultaneously determinded 23 fatty acids in bile by selected ion monitoring (SIM) method of gas chromatography-mass spectrometry (GC-MS). Biliary fatty acids were extracted by aminopropyl column and the extracts with (phospholipid fraction) or without (free fatty acid fraction) alkaline hydrolysis of phospholipid were derivatized with MSTFA/TMCS (N-methyl-N-trimethylsilyl-trifluoroacetamide/trimethylsilylchloride) mixture in order to be detected on the GC-MS. The recovery range of this method was 61.1-99.0% and the RSD value of within-a-day and day-to-day test were 3.1-25.6% and 3.8-27.0%, respectively. Using this method, biliary profile was investigated in the bile of normal controls and patients with gallstones. The amounts and their distribution of free and phospholipid fatty acids showed different pattern between normal subjects and patients.

• Journal of Nutrition and Health
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• v.19 no.3
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• pp.169-176
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• 1986

The effect of riboflavin feeding on lipid emtabolism in rat were studied. 70 male Sprague-Dawley rats were divided into 7 diet group ; control, riboflavin, cholesterol, MLHP (methyl linoleate hydroperoxide) cholesterol plus MLHP, cholesterol plus riboflavin, cholesterol plus MLHP plus riboflavin. After 4 weeks formular feeding sera of animals were collected, then checked cholesterol, triglyceride, phospholipid levels and lipoprotein by use of cellulose acetate papter. The results were as follows ; 1) Marked increase of triglyceride and phospholipid levels were found in the group feeding with cholesterol, cholesterol plus MLHP. 2) Combined riboflavin feeding prevented the increase of triglyceride and phospholipid levels in the group of cholesterol, cholesterol plus MLPH. 3) Also combined riboflavin feeding prevented the increase of $\beta$-lipoprotein fraction in cholesterol feeding group. Our results demonstrate that riboflavin has an inhibitory effects on the derangements of lipid metabolism due to administration of cholesterol, cholesterol plus MLHP.

• The Korean Journal of Pharmacology
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• v.5 no.1
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• pp.1-9
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• 1969

Alkaloidal fraction, a principle of ginseng extract was administered to rats orally in the amount of 2.5 mg per day per 100 gm body weight continuously for eight weeks to assess the lipid content of the serum and liver tissue. The findings can be summarized as follows: 1. Total cholesterol contents of serum and liver tissue were found to decrease with the administration of ginseng alkaloidal fraction. 2. Phospholipid contents increased in the serum and decreased in the liver tissue with the administration of ginseng alkaloidal fraction. 3. Triglyceride contents of the serum as well as liver tissue increased with the administration of ginseng alkaloidal fraction during the first 4 weeks but decreased in the second 4 weeks to reach a level below that of the pre-experimental period.

• Journal of Life Science
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• v.12 no.3
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• pp.233-241
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• 2002

Seasonal variation of uraenesox cinereus) muscle was investigated. Crude lipid content varied from 3.85 to 12.59 g/100g, comprising the highest content in November. The major fatty acids of total lipid, neutral lipid, and phospholipid were C16:0, C23:0, C16:1, C18:1, C20:5, and C22:6, but in phospholipid, Cl8:3n-6 was also the major fatty acid. The C22:6 content of the neutral lipid was much lower compared to that of the total lipid and phospholipid. The content of polyunsaturated fatty acids (ranged from 73.93 to 66.23%) in phospholipid was higher than that of any other lipid fraction. In glycolipid, C20:1 and C14:1 were higher compared to those of any other lipid fraction, but C20:5 and C22:6 were lower. The annual average ratio of n-3 to n-6 of total lipid, neutral lipid, phospholipid, and glycolipid was 10.82, 12.27, 6.63, and 6.50, respectively. The particular trend of seasonal variation of fatty acid composition was not showed in total and neutral lipid. However, the samples caught in September and November had a high crude lipid content with a significantly lower content of polyunsaturated fatty acids in phospholipid. Also, the content of monounsaturated fatty acids in glycolipid was lower in samples of September, November, and January.

• Journal of the Korean Society of Food Science and Nutrition
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• v.31 no.5
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• pp.814-818
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• 2002

The effects of dichloromethane (CH$_2$C1$_2$), ethylacetate (EtOAc) or water ($H_2O$) fraction of Korean cabbage kimhi on accumulation of lipids in the heart, kidney and lung of rabbit fed 1% cholesterol diet for 16 weeks were studied. The amount of kimhi fraction added to the 100 g of diet was 8.3 mg of CHaC12.5.6 W of EtOAc, and 221.9 mg of $H_2O$, which are equivalent to 5% of freeze-dried kimhi added to the diet. Each group had 6 rabbits and rabbit was housed individually. Cholesterol and triglyceride concentrations of three organs were noticeably decreased due to these solvent fractions of kimhi but for phospholipid and total lipids, only CH$_2$C1$_2$ fraction group showed decreasing effects. In the heart, compared to the control, cholesterol concentrations for CE$_2$C1$_2$, EtOAc, and H2O fraction group were decreased by 42 (P<0.05),21 (P<0.05), and 8%, respectively, and triglyceride for these groups were decreased by 29 (p<0.05),4, and 11%, respectively. In kidney, cholesterol concentration for CH$_2$C1$_2$, EtOAc, and H2O fraction group were decreased by 23, 12, and 11%, respectively (p<0.05) and triglyceride concentration for CH$_2$Cl$_2$ and H2O fraction groups were significantly decreased by 51 and 21%, respectively (p<0.05). In lung, cholesterol concentrations for CItCIB, EtOAc, and H2O fraction groups were decreased by 37,20, and 22%, respectively (p<0.05) and triglyceride concentration of these groups were significantly decreased by 39, 28, and 28%, respectively (p<0.05). And phospholipid and total lipid of CH$_2$CI$_2$ fraction group were significantly decreased by 33 and 34% respectively (p<0.05). Among three organs the cholesterol content of lung was the highest followed kidney and heart. For triglyceride, heart, kidney and lung is in the order of showing the highest concentration. The phospholipid concentration was not significantly different among three organs. According to these results, we may conclude that CH$_2$C1$_2$, fraction of kimhi might have the most active component, which decreases cholesterol, triglyceride, phospholipid and total lipids concentrations in heart, kidney, and lung of rabbit fed high cholesterol diet.

• Korean Journal of Veterinary Research
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• v.35 no.4
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• pp.713-727
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• 1995

The present study was investigated fatty acid composition of the phospholipid in the RBC membrane, liver and microsomal fraction after vitamin E and/or insulin treatment to evaluate the effect of vitamin E on the oxidative stress in STZ-treated rat and BB rat. Results obtained through the experiments were summarized as follows; 1. Effect of vitamin E and/or insulin treatment in STZ-treated rat 1) In the insulin treated group and the combination treated groups of vitamin E with insulin, body weights were increased compared to STZ-treated rat(STZ control group). Especially it was more significantly increased in the combination treated group of high dose vitamin E with insulin. 2) The composition of fatty acids of the phospholipid in RBC membrane, liver and microsomal fractions was shown a decreased C16:1, C18:1, C20:4 and an increased C16:0, C18:0, C18:2 in STZ control group compared to normal control group. In RBC membrane, liver and microsomal fractions after vitamin E with insulin treatment in STZ-treated rat, effect on the composition of fatty acids of the phospholipid was shown the result of a decreased C16:0, C18:0, C18:2 and an increased C16:1, C18:1, C20:4. 3) Hemolysis rate of the RBC to $H_2O_2$ was increased in the STZ control group and it was decreased below the hemolysis level of normal control group by vitamin E treatment. 2. Effect of vitamin E and/or insulin treatment in BB rat 4) Only in microsomal fraction, fatty acid composition was different between insulin treatment group and vitamin E with insulin treatment group. It was increased C16:0 and C18:1, and decreased C18:0 and C18:2 in vitamin E with insulin treatment group: But C20:4 was not different in two groups. These results suggest that the combination treatment of vitamin E and insulin could prevent the oxidative change of fatty acids in P-lipid of the RBC membrane, liver and microsomal fraction in STZ-treated rats and BB rats.

• Journal of the Korean Society of Food Science and Nutrition
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• v.13 no.3
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• pp.259-262
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• 1984

In order to examine the effect of oil extraction methods on the charateristics of sesame oil, the fractionation pattern and fatty acid compositions of the each fraction of the oil were compared in the oil extracted by the three different extraction methods, that is, pressure extraction of roasted seed (RTP), acetone extraction of roasted seed (RTE) and acetone extraction of raw seed (RWE). The amount of triglyceride in RWE oil was slightly higher as 77.2% of the total lipid than that in RTP and RTE oil of 73.4%. The content of oleic and linoleic acid in glycolipld and fatty acid fractions, linoleic acid in triglyceride, and palmitic and stearic acid in phospholipid fractions from RTP and RTE oil were higher than those from RWE oil, but the oleic acid in triglyceride. Palmitic and stearic acid in free fatty acid, and oleic and linoleic acid in phospholipid from RTP and RTE oil were lower than those from RWE oil. The ratio of $C_{18}$ to $C_{16}$ fatty acids and unsaturated to saturated fatty acids in glycolipid and phospholipid fractions from RTP and RTE oil were higher than those from RWE oil, but the ratios in triglyceride and free fatty acid fractions from RTP and RTE oil were lower than those from RWE oil.