• Journal of the Korea Organic Resources Recycling Association
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• v.13 no.1
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• pp.79-89
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• 2005

Forest soils were analyzed on their biological and physicochemical properties for the ecological restoration of burnt forest soil using organic wastes and proper microorganisms. Three kinds of soil samples were collected from undamaged soil(US), naturally restoring soil(NS) and artificially restoring soil(AS). All soil samples were sandy soil and acidic soil, ranged pH 5.34~5.78. Moisture content was higher in the soil of NS region. And the others were similar. Total organic matter and soluble sugar were higher at the surface, generally. Heterotrophic soil microbes were abundant at the surface soil of NS and subsoil of AS. Dehydrogenase, cellulase and phosphatase activities were higher at the NS soil. Especially, Dehydrogenase activity as primary index of soil microbial process showed high correlationship with moisture content(r=0.90, P < 0.05).

• Journal of the Korean Society of Food Culture
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• v.19 no.4
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• pp.419-428
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• 2004

The Purpose of this study was to investigate the effect of dietary sea tangle in diabetic rats treated with streptozotocin(STZ). Four groups of rats (Sprague-Dawley male rats,180-200g) were normal rats fed control diet(C), diabetic rats fed control diet(CD), normal rats fed sea tangl diet(T), and diabetic ,rats fed sea tangle diet(TD), diabetes was induced by single injection of streptozotocin(60mg/kg B.W.). High density lipoprotein(HDL) of T and TD group were higher than other groups(C and T groups). And the weekly change of blood sugar was decreased in the 3th and 4th weeks. But serum triglyceride (TG) of diabetic rats fed sea tangle diet(TD) was lower than diabetic rats fed control dlet(CD). Activity of hepatic microsomal Glucose 6-phosphatase(G6Pase) was significantly increased CD and TD groups higher than C and T groups. Hepateic glutathione S-transferase(GST of T, CD and TD groups were significantly lower than C group(p<0.05), glutathione peroxidase (GPX) of T and TD groups were significantly higher than C and CD groups(p<0.05).

• Journal of Plant Biology
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• v.35 no.1
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• pp.91-95
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• 1992

Changes of contents of reducing sugar and sucrose and activities of sucrose-phosphate synthase, sucrose synthease and invertase from the leaves of barley (Hordeum vulgare L. cv. Chalssal) seedlings grown at $4^{\circ}C$ were investigated, and the property of acid invertase were also examined. In the seedlings grown at $4^{\circ}C$ for 3 days, the contents of reducing sugar and sucrose were increased to 1.3 and 2.4 times, respectively. Activity of acid invertase was decreased markedly by cold treatment while the activities of sucrosephosphate synthase, sucrose synthase, and alkaline invertase were not changed. In acid phosphatase purified partially by ammonium sulfate fractionation and DEAE-Sephacel column chromatography, the $K_m$ value for sucrose was 9.5 mM and the optimum pH and temperature was 5.5 and $35^{\circ}C$ respectively. This enzyme was supposed to be ${\beta}-fructosidase$ by studies on the substrate specificity and the molecular weight was estimated to be 63 Kd by Sephadex G-200 gel chromatography.graphy.

• The korean journal of orthodontics
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• v.36 no.6
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• pp.465-473
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• 2006

Objective: This study evaluated the effects of nitric oxide (NO) on the proliferation and differentiation of human periodontal ligament cells involved in orthodontic tooth movement. Methods: A range of concentrations of sodium nitroprusside (SNP), a NO donor, were administered to samples of human periodontal ligament cells, followed by measurement of cell viability, alkaline phosphatase (ALP) activity, and expression of osteonectin and bone sialoprotein. Results: Cell viability, ALP activity and the expression of osteonectin and bone sialoprotein were increased in human periodontal ligament cells treated with SNP concentrations of < 0.2 mM compared with controls, but were decreased with SNP concentrations of > 1.0 mM. Conclusion: NO has a biphasic effect on proliferation and differentiation in human periodontal ligament cells, with a stimulatory effect at low concentrations and an inhibitory effect at high concentrations.

• Journal of the East Asian Society of Dietary Life
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• v.16 no.2
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• pp.165-173
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• 2006

To investigate the effects of yellow soybean powder on serum lipid level and enzyme activities, male Sprague-Dawley(SD) rats were given hyperlipidemic diets for 6 weeks. Rats(n=8) were divided into three groups consisting the normal group(N), hyperlipidemic diets group(C) and hyperlipidemic diets and yellow soybean powder group(YSP). YSP group lowered triglyceride, total lipid, phospholpid, ester-cholesterol and free-cholesterl than control group. YSP group lowered total-cholesterol, VLDL-cholesterol and LDL-cholesterol but increased HDL-cholesterol of the serum compared to the control group. Athergenic Index(AI) and LHR(LDL-cholesterol/HDL-cholesterol ratio) of YSP group was decreased by control group (p<0.05). On the other hand, glutamic oxaloacetate transaminase(GOT), glutamic pyruvate transaminase (GTP), lactate dehydrogenase(LDHase) and alkaline phosphatase(ALP) enzymes activities were decreased by hyperlipidemic diets group (C). These results suggest that yellow soybean powder fed a lipid lowering effect in serum of rats.

• Microbiology and Biotechnology Letters
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• v.17 no.4
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• pp.301-306
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• 1989

A genomic library of Zymomonas mobilis DNA was constructed in Escherichia coli using plasmid pUC9 Allyl alcohol was used to screen a genomic clone expressing alcohol dehydrogenase. The plasmids isolated from two clones, which were sensitive to allyl alcohol, were found to be related and to share a common 2.6 kb fragment encoding alcohol dehydrogenase II identified as one of two isozymes in Z. mobilis by staining for alcohol dehydrogenase activity on polyacrylamide gel and spectrophotometric analysis of several substrate oxidations.

• Journal of Life Science
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• v.10 no.5
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• pp.490-495
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• 2000

Effect of Quartz porphyry (QP) on clinical chmical parameters in male common finch by feeding the basal diet (Control group) containing 3.0% QP (QP group) for 14 days was studied. Body weight gain, feed intake, feed efficiency, the relative weight (mg/10 g body wight) of liver and heart were not significantly different in the both groups. However, the relative weights of kidney and gizzard in the QP group were higher and lower than in the control group, respectively. The contents of triglyceride, cholesterol, total protein, glucose and albumin, and the activity of glutamic pyruvic transaminase (GPT) in serum were not significantly different in the both groups. The activities of alkaline phosphatase (ALP) and glutamic oxaloacetic transaminase (GOT) in serum were lower and higher in the QP group than in the control group.

• Korean journal of food and cookery science
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• v.28 no.3
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• pp.311-318
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• 2012

The correlation between osteoporosis and reactive oxygen species (ROS)-induced oxidative stress was investigated. Thus, interest in food and plants with antioxidant effects that can reduce damage caused by ROS during bone metabolism is heightening. In this study, the antioxidant effect of Taraxacum mongolicum on proliferation and differentiation of MC3T3-E1 cells under H2O2-induced oxidative stress was studied to investigate its protective effect against oxidative stress and its availability as an antioxidant material related to bone diseases. As a result, total polyphenol and total flavonoid contents of T. mongolicum were 33.65 mg/g and 4.45 mg/g, respectively. The T. mongolicum extract increased proliferation of both MC3T3-E1 cells and differentiated osteoblasts under $H_2O_2$-induced oxidative stress conditions. In addition, two differentiation markers, alkaline phosphatase activity and mineralization level in the T. mongolicum extract, tended to increase. These results indicate that T. mongolicum extract suppressed the damage to osteoblasts under oxidative stress and that it is potential antioxidant materials for preventing bone diseases.

• Environmental Analysis Health and Toxicology
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• v.12 no.3_4
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• pp.61-66
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• 1997

Recently, incidences of pulmonary-related diseases are increasing and natural ecosystem is destroyed year by year owing to air pollutions by industrial noxious gases such as sulfur dioxide and carbon dioxide, etc. Sulfur dioxide produced by oxidation of sulfur compounds is water soluble and causes several kinds of pulmonary diseases, damaging on plants and animals. This study was undertaken to investigate effects of acute exposure of rats to sulfur dioxide using biochemical parameters. The sAST (aspartate transaminase) activity was not significantly affected but sALT (alanine transaminase), sLDH (lactate dehydrogenase) and sALP (alkaline phosphatase) activities were significantly increased by acute sulfur dioxide gas exposure. Also sulfur dioxide gas treatment significantly diminished serum cholinesterase activity. The total lipid, triglyceride, total-, HDL- and LDL-cholesterol contents were significantly increased by sulfur dioxide gas exposures. But the body and liver weight were generally diminished in comparison with control group. Our results' showed increased enzyme activities and lipid contents, but body and liver weight were all diminished by sulfur dioxide gas treatment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.9
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• pp.1145-1152
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• 2009

For the utilization of Korean ginseng (Panax ginseng C.A. Meyer) in the functional drink, we prepared the fermented Korean ginseng with mushroom mycelia (Ganoderma lucidum; WG-GL, Hericium erinaceum; WG-HE and Phellinus linteus; WG-PL) by solid culture. A proximate analysis showed that the fermented Korean ginseng contained significantly more crude fat (4.66$\sim$12.02%) than Korean ginseng (WG, 1.61%) whereas crude protein content of WG (13.64%) was higher value than those of the ferments (7.60$\sim$12.57%). When we also evaluated effects of the fermented Korean ginseng on the mitogenic activity, hot-water extract from WG-PL was significantly higher than those of WG or mycelia only fermentation (GL, HE and PL) as analyzed by IL-2 production (1.64-fold of the saline control) and proliferation of splenocytes (1.47-fold). In addition, the lysosomal phosphatase activity (WG-HE; 1.32-fold) and NO/TNF-$\alpha$ production (WG-HE; 2.27-fold of the saline control at 50 ${\mu}g$/mL, WG-PL; 3.56-fold, respectively) from macrophage in the presence of the fermented Korean ginseng were higher than those of WG or mycelia fermentation. These results indicate that hot-water extracts from the fermented Korean ginseng with mushroom mycelia by solid culture contain chemical ingredients different from the Korean ginseng, and that it might provide beneficial immunostimulating activity.