Effects of Dietary Sea Tangle on Blood Glucose, Lipid and Glutathione Enzymes in Streptozotocin-Induced Diabetic Rats

다시마 분말이 당뇨 유발쥐의 혈당과 지질농도 및 항산화 효소계에 미치는 영향

  • Cho, Young-Ja (Department of Food & Nutrition, Mokpo National University) ;
  • Bang, Mi-Ae (Department of Food & Nutrition, Mokpo National University)
  • 조영자 (목포대학교 생활과학부 식품영양전공) ;
  • 방미애 (목포대학교 생활과학부 식품영양전공)
  • Published : 2004.08.31

Abstract

The Purpose of this study was to investigate the effect of dietary sea tangle in diabetic rats treated with streptozotocin(STZ). Four groups of rats (Sprague-Dawley male rats,180-200g) were normal rats fed control diet(C), diabetic rats fed control diet(CD), normal rats fed sea tangl diet(T), and diabetic ,rats fed sea tangle diet(TD), diabetes was induced by single injection of streptozotocin(60mg/kg B.W.). High density lipoprotein(HDL) of T and TD group were higher than other groups(C and T groups). And the weekly change of blood sugar was decreased in the 3th and 4th weeks. But serum triglyceride (TG) of diabetic rats fed sea tangle diet(TD) was lower than diabetic rats fed control dlet(CD). Activity of hepatic microsomal Glucose 6-phosphatase(G6Pase) was significantly increased CD and TD groups higher than C and T groups. Hepateic glutathione S-transferase(GST of T, CD and TD groups were significantly lower than C group(p<0.05), glutathione peroxidase (GPX) of T and TD groups were significantly higher than C and CD groups(p<0.05).

다시마 분말 첨가식이가 당뇨쥐의 당질과 지질대사 및 항산화효소계에 미치는 영향을 규명하기 위하여 SD계 횐쥐에 17% 다시마 분말을 급여하여 7주간 실험 사육한 후 혈청의 포도당 및 지질농도와 주요장기의 항산화효소의 활성도를 관찰하였다. 다시마 당뇨군(TI)에서 다사마 섭취는 당뇨에 의한 체중감소 현상을 보였다. 당뇨군의 혈당 농도는 다시마 섭취에 의한 혈당강하 효과는 3주째에 나타났다. 당뇨에 의한 산화적 스트레스로 인해 증가한 GST 활성도가 다시마 섭취로 인해 정상 수준으로 저하됨으로써 다시마의 섭취가 당뇨시 산화적 반응에 대한 방어기전을 제공할 수 있음을 유추할 수 있었다. 또한 중성지방이 감소하고 HDL-콜레스테롤의 증가하는 경향으로 심혈관계와 관련되는 당뇨합병증의 억제 가능성을 제시하였다.

Keywords

References

  1. Lahaye M. Marine algae as sources of fibers : Determination of soluble and insolubl dietary fiber contents in some sea vegetabls. J Sci Food Agric. 54 : 587-594, 1991 https://doi.org/10.1002/jsfa.2740540410
  2. Ito K, Tsuchiya Y. The effect algal polysccarides on the depressing of plasma cholesterol levels in rats. In Proc of 7th Int Seaweed Symp Nishizawa K et Univ Tokyo Press, Japan, pp 558-561, 1972
  3. Kim DS, Park YH. Uronic add composition, block structure and some related properties of alginic add. J Korean Fish Soc 18 : 29-36, 1985
  4. Colliec S, Fischer AM, Tapon-Bretaudiene J, Boisson C, Durand P, Jozefonvcz J. Anticoagulant properties of a fucoidam fraction. Thromb Res 64: 143-154, 1991 https://doi.org/10.1016/0049-3848(91)90114-C
  5. Park SH, Lee HS. Effects of legume supplementation on the glucose and metabolism and lipid peroxidation in streptozotocin-induced rats. J Korean Nutr Soc 36(5): 425-436 , 2003
  6. Vuksan V, Korsic M, Posavi AA. Metabolic disease and the high-fiber diet. Lejec Vjesn 119 : 125-127, 19970
  7. Krashenisa GM, Botvineva LA, Mogila AV. Effectiveness of increased contents of dietary fiber in early sugars of non-insulin dependent diabetes mellitus. Vopr Pitan 4: 35-37, 1994
  8. Jenkins DJA, Leeds AR, Gasssull AR, Coche B, Alberti GMA. Decrease in postpriandial insulin and glucose concentration by guar and pectin. Ann Intern Med 86 : 20-23, 1977
  9. Goldberg RR. Lipid disorders in diabetes. Diabetes care 4 : 561-572, 1981 https://doi.org/10.2337/diacare.4.5.561
  10. Reaben KM. Abnomal lipoprotein metabolism in noninsulin-dependent diabetes mellitus Am J Med 83:31-40, 1987 https://doi.org/10.1016/0002-9343(87)90801-1
  11. West KM, Ahuja MMS, Bennet PH, et al. The role of circulating glucose and triglyceride concentration and their interaction with other risk factors as determinationds arterial dierase in nine diabetic populnation samples from the WHO multinational study. Diabetes Care 6: 361-369,1983 https://doi.org/10.2337/diacare.6.4.361
  12. Laybutt DR, Kaneto H, Hasenkamp W, Grey S, Jonas JC, Sgroi DC, Groff A, Ferran C, Bonner-Weir S, Sharma A, Weir GC. Increased Expression of Antioxidant and Antiapoptotic Genes in Islets That May Contribute to [beta]-Cell Survival During Chronic Hyperglycemia. Diabetes 51(2) : 413-423, 2002 https://doi.org/10.2337/diabetes.51.2.413
  13. Reddi AS. Riboflavin nutritional status and flavoprotein enzymes in streptozotocin-diabetic rats. Biochim Biophys Acta 882(1) : 71-6, 1986
  14. Melo SS, Arantes MR., Meirelles MS, Jordao AA Jr, Vannucchi H., Lipid peroxidation in nicotinamide-deficient and nicotinamide-supplemented rats with streptozotocin-induced diabetes. Acta Diabetol 37(1): 33-9,2000 https://doi.org/10.1007/s005920070033
  15. Rerup CC. Drugs producing diabetes through damage of the insulin secreting cells, Pharmacol Rev 22 : 485-518, 1970
  16. Bucolo G, Gavid H. Quantitative determination of serum triglyceride by use of enzymes. Clin Chem 19 : 476-482, 1973
  17. Friedwald WT, Levy RL, Fedreicson DS, Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without was of the preparative ultracentrifuge. Clin Chem 18 : 499-506, 1972
  18. Baginski ES, Foa PP, Zak B. Glucose 6-phosphatase In Methods of Enzymematic Analysis 2, Academic Press, New York, pp 876-880, 1983
  19. Buege JA, Aust SD, Sidney F, Lester P. Methods in Enzymology 52, Academic Press, New York, pp 302-310,1978
  20. Habig WH, Pabst MJ, Jakoby WB. Glutathione Stransferase. J.Biol. Chem. 249: 7130-7139, 1974
  21. Tappel AL. Glutathione peroxidase and hydroperoxides, In Methods in enzymology(Fleisher S & Packer L ed) 52 : 506-513, 1978
  22. Lowry OH, Rosebrough NJ, Farr AL, Randall RT. Protein measurement with the folin phenol reagent. J Biol Chem, 193 : 265-275, 1951
  23. Lee HS, Choi MS, Lee YK, Park SH, Kim YJ. A study on the development of high-fiber supplements for the diabetic patients(I). J Korean Nutr 29(3) : 286-295, 1996
  24. Mogensen CE, Anderson MJF. Increased kidney size and glomerular filtration rate early juvenile diabetes. Diabetes 22; 706-712, 1973
  25. Heaton KW. Food fiber as an obstacle to energe intake. Lancet 2 : 1418-1421, 1973 https://doi.org/10.1016/S0140-6736(73)92806-7
  26. Seyer-Hansen K. Renal hypertrophy in streptozotocin-diabetic rats. Clin Sci Mol Med 51 : 551-555, 1973
  27. Seyer-Hansen K. Renal hypertropy in experimental diabetas : Relation to severity of diabefes. Diabetologia 13; 141-143, 1977 https://doi.org/10.1007/BF00745142
  28. Lee JS, Son HS, Maeng YS, Chang YK. Ju JS . Effects of buckwheat on organ weight, glucose and lipid metabolism in streptozotocin-induced diabetic rats. Kor J Nutr 27(8) : 819-827, 1994
  29. Lee HS, Choi MS, Lee YK, Park SH, Kim YJ. A study on the development of high-fiber supplements for the diabetic patients(1). J Korean Nutr 29(3) : 296-306, 1996
  30. Tol AV . Hypertriglyceride in the diabetic rat Deffective removal of serum very low density lipoprotein. Atherosclerosis 26 : 117-128, 1971 https://doi.org/10.1016/0021-9150(77)90146-0
  31. Bar-on H, Levy E, Oschry Y, Ziv E, Scafrir E . Removal deffect of very low density lipoproteins from diabetic rats. Biochem Biophysi Acta 793 : 115-118,1984
  32. Mithieux G, Vidal H, Zitoun C, Bruni N, Daniele N, Minassian C. Glucose-6-phosphatase mRNA and activity are increased to the same extent in kidney and liver of diabetic rats : Diabetes, 45(7):891-6, 1996 https://doi.org/10.2337/diabetes.45.7.891
  33. Ghosh R. Mukherjee B, Chatterjee M. A novel effect of selenium on streptozotocin-induced diabetic mice. Diabetes Res 25(4):165-71, 1994
  34. Kedziora-Kornatowska K, Luciak M. Effect of aminoguanidine on lipid peroxidation and activities of antioxidant enzymes in the diabetic kidney iochem Mol Biol Int Oct;46(3):571-83, 1998
  35. Celik S, Baydas G, Yilmaz O. Influence of vitamin E on the levels of fatty acids and MDA in some tissues of diabetic rats. Cell Biochem Funct. 20(1):67-71,2002 https://doi.org/10.1002/cbf.936
  36. Kinalski M, Sledziewski A, Telejko B, Zarzycki W, Kinalska I. Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes. Acta Diabetol 37(4):179-8, 2000 https://doi.org/10.1007/s005920070002
  37. Bae EA, Kwun TW, Moon KS. Isoflavone contents and antioxidative effects soybean curd and their by products. J Korean Society of Food Science and Nutrition 26(3): 371-375,1997
  38. Kitahara JF, Satoh K, Nishimura K, Ishikwa T, Ruike K, Sati K, Tsuda H, Ito N. Changes in molecular from of rat hepatic glutathione S-transferase during chemical hepatocarcinogenesis. Cancer Res.44: 2698-2703, 1984
  39. Lim EY, Kim H. Effect of taurine supplement on the lipid peroxide formation and the activity of glutathione-dependent enzyme in the liver and Islet of diabetic model mice. J. Korean. Soc. Food Nutr 24(2),195-201, 1995
  40. Kinalski M, Sledziewski A, Telejko B, Zarzycki W, Kinalska I.Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes. Acta Diabetol 37(4):179-8, 2000
  41. Kesavulu MM, Giri R, Kameswara Rao B, Apparao C. Lipid peroxidation and antioxidant enzymes levels in type 2 diabetics with microvascular complication. Diabetes Metab 26(5): 387-392, 2000
  42. Kakkar R, Kala J, Mantha SV, Prasod K. Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Molecular and Celluar Biochemistry 151: 113-119,1995 https://doi.org/10.1007/BF01322333
  43. Kitahara JF, Yamazaki T, Isikawa T, Camba EA, Sato K Change in activities of glutathione reductase during chemical hepatocarcinogenesis. Gunn 74: 649-655,1983