• The Korean Journal of Food And Nutrition
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• v.36 no.6
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• pp.551-560
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• 2023

This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25℃), shaking extraction (SHE, 80℃), and sonication assisted extraction(SAE, 25℃) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.197-197
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• 2017

In order to reduce the loss of perilla seeds at harvesting stage, different seeding methods and harvesting time had been applied to upland soil in NICS (National Institute of Crop Science), RDA, Milyang, Korea. The seeding methods were divided into hill and drill seeding, and harvesting time were 40%, 60%, 80%, and 100% color-changing of top cluster perilla (cv Deulsaem) seeds. Higher plant height and lowest flower cluster, thinner culm thickness was observed in drill seeding treated plot than hill seeding treated plot. However, field lodging occurred lower at drill seeding treatment (lodging degree 3) than hill seeding plot (lodging degree 5). Harvesting time affected the loss of perilla seeds and the loss rate of perilla seeds were investigated at different color-changing of top flower cluster. When seeds were harvested at 40%, 60%, 80%, and 100% color-changing of top cluster perilla seeds, the loss rate of hill and drill seeding showed no significant differences however the loss rated of 40, 60, 80, and 100 % color-changing of top flower cluster were 1.9% to 3.0%, 3.8% to 3.9%, 8.0% to 10.2%, and 16.1% to 22.7%, respectively. The harvesting time had no significant effects on the yield of perilla seeds. These results suggest that optimum harvesting time of perilla could be recommend at the 60% color-changing of top cluster to reduce the loss of perilla seeds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.49 no.3
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• pp.222-226
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• 2004

This study was conducted to measure the major agronomic characteristics of 85 local varieties of perilla at Chungbuk Agricultural Research and Extension Services in 2001. Perilla accessions examined were classified into three maturity groups, i.e, early, medium, and late maturity group of 19 (23％),57 (67％), and 9 (10％) accessions, respectively. The early and late maturity groups mainly consisted of collections from middle-northern area and southern area, respectively, while the medium group consisted of accessions from all over the country. Average 1,000-seed weight (TSW) was 2.7 g. TSW of the collection from Hamyang was the greatest a 3.9 g, while the TSW of Pyungchang collection was the smallest as 1.7 g, and most of collections produced medium and small seeds. Perilla accessions with greate 1,000-seed weight seemed to be belonged to the late maturity group. Seed coat colors of perilla accessions were dark brown (30％), brown (55％) and gray brown (6％), respectively, Among seed coat colors, brown color consisted of 91％. Stem height, the number of nodes, branches, flower clusters, and capsules per flower cluster, and the length of flower cluster were positively correlated occ, while these characteristics were negatively correlated with the number of capsules per flower cluster and 1,000-seed weight. These results allowed us to select 5 perilla collections, containing 1 collection with gray white seed color, and 4 collections over 2.5g 1000 seed weight, as parental lines in the breeding program.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.543-547
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• 1995

This study was conducted to light intensity and night interruption on leaf production in perilla. Using two cultivars ; og-dong and Yeup-sil. The present studies were conducted to investigate effect of the light intensity and night interruption for prolongation of vegetative growth by flowering inhibition in the National Honam Crop Experiment Station, R. D. A, Iri, Korea. The results are summarized as followes, varietal differences of days to flower initiation were not significant by the light intensity and night interruption, however differences of days to flower initiation light intensity or night interruption treatment were higly significant. Flowering of perilla was prolongated in high light intensity and in long night interruption. Between the factors treated flowering of perilla was more influenced by light intensity than night interruption. Days to flower initiation of perilla were light intensity(0.5∼1 Lux) in 30 mins night interruption, however 3∼10 light intensity in 60 mins night interruption and 30∼100 Lux light intensity in 10, 30, 60 mins night interruption treatment were not flowering. Plant height, leaf area and dry weight of perilla were the highest in 30∼100 Lux light intensity treatment than in the other treatments. The results would be avaliable establish year-round production methods for low cost of perilla leaf.

• Korean Journal of Plant Resources
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• v.18 no.1
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• pp.179-185
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• 2005

This study was conducted to evaluate the major agronomic characteristics Korean of 85 and Chinese Jilim Province of 5 local varieties of perilla at Chungbuk Agricultural Research and Extension Services in 2001. Perilla accessions examined were classified into three maturity groups, i.e, early, medium, and late maturity group of $19\;(23\%),\;57\;(67\%)$, and $9\;(10\%)$ accessions, respectively. The early and late maturity groups mainly consisted of collections from middle-northern area and southern area, respectively, while the medium group consisted of accessions from all over the country. But chinese Jilim collection wad all mediumgroup. Average 1,000-seed weight(TSW) was 2.7 g. TSW of the collection from Hamyang was the greatest a 3.9g, while the TSW of Pyungchang collection was the smallest as 1.7g, and most of collections produced medium and small seeds. Perilla accessions with greate 1,000-seed weight seemed to be belonged to the late maturity group. Chinese collection was 2.2 to 3.19g and overage was 2.6g in average. Accodingly TSW hadn't more variegation than Korean. Seed coat colors of gorilla accessions were dark brown$(30\%)$, brown$(55\%)$ and gray brown$(6\%)$, respectively. Among seed coat colors, brown color consisted of $91\%$. Chinese was dark brown$(20\%)$, brown$(80\%)$ in color. Positively correlated that stem height, the number of nodes, branches and flower clusters with Korean and Chinese collection, capsules per flower cluster and the length of flower cluster with Korean collection and 1,000-seed weight and maturing period, flower clusters, stem height and the number of nodes with Chinese collection. And negatively correlated that the number of capsules per flower cluster and 1,000-seed weight with Korean collection and the length of flower cluster, stem height, branches, the number of nodes and flower clusters with Chinese collection.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.466-472
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• 1997

Vegetable perilla, "Ipdlkkae 1"(Perilla frutescens var japonica Hara), was tested about the flowering and maturing responce in summer and winter. In summer season, it was researched about those responses according to the change of seeding date from May 15th to Oct. 15th at one month interval in the field. "Ipdlkkae 1" flowered Oct. 2nd under the day length of eleven hours and fourty-one minutes, compared with Sep. 6th (day length of twelve hours and fourty-three minutes) of "Yepsildlggae". And those responses showed that vegetable perilla was have to seeded before July 15th for two reason. The first is a unique response of perilla to day length. If perilla stay under short-day condition for some days, perilla will flower after four weeks. The second is a weather, especially frost and cold. In the test of latest seeding at Oct. 15th, the plants flowered more late than normal flowering period and they were not able to mature for frost of early winter. And this result showed that any other species, which has the characteristic of later flowering than that of "Ipdlkkae 1", could not able to mature in the field. In winter time, this species was tested about the same responses according to the change of short-day treatments. In the case of the test from May 1st (above fourteen hours day length), even if the test plants were stayed under short-day condition for more than 10 days, they were not able to mature, but flowerd. From the test of Apr. 15th, day length of thirteen hours, the plants were showed variable reaction to the short-day treatment. In this test, 11days for short-day treatment was a basic day to decide whether flowering was delayed or not. In the test from Apr. 1st, perilla seeds were able to harvest at least 5 days short-day treatment. In the final test from Mar. 15th, it had no need to take short-day treatment for harvesting of normal seeds, because the day length of that are twelve hours, which is an enough time to induce flowering and maturing, previously reported.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.46 no.5
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• pp.395-400
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• 2001

We investigated the variations of the flowering response to daylength in Perilla crop (var. frutescens). Seventeen accessions of Perilla crop and one accession of weedy type of var. crispa from China, Korea and Japan were cultivated under three daylength conditions, i.e., short-days, natural daylength and long-days. Most accessions of Perilla crop from China, Korea and Japan were divided into three types, early maturing type, intermediate maturing type and late maturing type by their natural flowering habit. In most of the accessions used, the flowering habit was significantly accelerated by short-day conditions and was delayed by long-day conditions. All the accessions of Perilla crops flowered within 57 days under the 10 hrs light treatment, whereas they did not flower at all even at 170 days after sowing under the 16 hrs light treatment. Thus, this finding suggested that there is a relationship between the types of flowering response to daylength and the geographical distribution which determines the planting season in traditional cultivation practices of Perilla crops. Positive correlation was observed between days to flowering and plant height or internode number in both the short-day and natural daylength conditions. Whereas, correlation was negative between days to flowering and inflorescence length or floret number in natural daylength condition, but it was positive in the short-day condition. Therefore, the daylength condition is considered as the most important environmental factor for flowering habit and morphological characters of Perilla crops. Flowering habit is considered as an important key character for the study of geographical differentiation of Perilla crop in East Asia.

• Korean Journal of Agricultural and Forest Meteorology
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• v.4 no.2
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• pp.80-85
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• 2002

This study was conducted to investigate the effects of night illumination on the growth and yield of sesame(Sesamum indicum) and perilla(Perilla frutescens). Stem length and height of the first pod in sesame increased, whereas number of branch was reduced and flowering date and maturing date delayed as light intensity of night illumination increased. Number of capsules per plant, ripening ratio and seed yield of sesame were reduced as light intensity of night illumination increased. Yield reduction was greater in Ahnsankkae than in Sodunkkae. Number of nodes increased under night illumination in perilla. Two test cultivars showed reduction in number of branch at 6~10 Lx(22 ㎽ m$^{-2}$ ) intensity of night illumination, even though there was a variation between cultivars. Perilla was sensitive to night illumination far flowering and ripening. Flowering date was delayed by 21 to 28 days at 6~10 Lx intensity of night illumination. Number of flower clusters, number of seeds per flower cluster, and 1,000-seed weight in perilla were significantly reduced and seed yield was reduced by 89 to 98% at 6-10 Lx intensity of night illumination.

• Horticultural Science & Technology
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• v.35 no.4
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• pp.510-522
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• 2017

To better understand the morphological variation for Perilla crop in different areas of China, we studied the morphological variation in 87 accessions (84 cultivated var. frutescens and three cultivated var. crispa) from high latitude (Northeast China) and middle latitude (North and Northwest China) areas of China by examining seven quantitative and 10 qualitative characters. Analysis of the morphological variation determined that there was significant morphological differences in five quantitative traits between cultivated var. frutescens and cultivated var. crispa, including effective number of branches (QN2), number of internodes (QN3), number of branches (QN4), length of the largest inflorescence (QN5), and days from germination to flowering (QN7). However, two quantitative traits-plant height and number of florets of the largest inflorescence-did not show any significant differences between cultivated var. frutescens and cultivated var. crispa. In addition, significant differences for six quantitative traits were found between the accessions of cultivated var. frutescens originating from high and middle latitude areas in China, which included QN2, QN3, QN5, number of florets of the largest inflorescence (QN6), and QN7. Principal components analysis (PCA) identified five quantitative characters [plant height (QN1), QN2, QN3, QN4, QN7] and six qualitative characters [fragrance of plant (QL1), color of reverse side of leaf (QL3), degree of pubescence (QL5), color of flower (QL6), shape of leaf (QL7), and hardness of seed (QL10)] that contributed to the positive direction on the first axis. The other quantitative and qualitative characters contributed to the negative direction on the first axis. Most accessions of cultivated var. frutescens and cultivated var. crispa were clearly separated by the first axis. In addition, most accessions of cultivated var. frutescens are from high latitude and middle latitude areas that were clearly separated by the first axis, except for several accessions. The findings from this study will provide useful information towards understanding the morphological variation of Perilla crop according to geographical distribution in high and middle latitude regions of China.

• Korean Journal of Breeding Science
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• v.43 no.6
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• pp.616-619
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• 2011

'Dayu', a perilla (Perilla frutescens (L.) Britton) cultivar for edible seed was developed by Department of Functional Crop, NICS, RDA in 2004. It was developed from a cross between a pedigree of Daeyeop as a female and YCPL1846 as a male parent in 1994. 'Dayu' could be characterized by white flower color and brown grain color. Dayu shows 127 cm in plant height, and has lodging tolerance. Maturing date of 'Dayu' was October 6, which was similar with that of 'Saeyeopsil'. This new cultivar has high oil content with 48.5% and high linolenic acid in the fatty acid composition. The grain yield potential of 'Dayu' is about 1.44 MT/ha in the regional yield trial.