• Korean Journal of Veterinary Service
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• v.21 no.1
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• pp.13-20
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• 1998

This survey was carried out to detect the residual antibiotics and sulfonamides in emergency slaughtered cattle(n=265) from slaughter houses in Kyeonggi province by EEC 4-plates method, Charm II and HPLC. The results were summarized as follows ; 1. Antimicrobial substances were detected from 24 samples(9.1%) by EEC 4-plates method and the detection ratio were highest in summer(13.8%). 2. Twenty-two of the 24 positive samples were classified as sulfonamide(34.4%), TCs(31.3%), $\beta$-lactam(23.5%) and aminoglycoside(9.3%) by Charm II test. 13(59.1%) of the 22 samples contained single agent and 9 samples(40.9%) contained 2 or more agents. 3. Oxytetracycline(27.3%), penicillin G (27.3%) and sulfathiazole(18.2%) were detected in 20 from 22 samples by the HPLC and Charm II test. 4. The residual concentration of oxytetracycline, penicillin G and sulfonamide were 0.29~9.30 ppm, 0.05~9.58 ppm and 0.04~7.59 ppm, respectively and 19 samples(7.2%) were exceeded tolerance levels.

• Advances in Traditional Medicine
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• v.5 no.1
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• pp.57-61
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• 2005

Ethanol and aqueous extracts of Cassia auriculata were tested in vitro against fungi (Candida albicans and Microsporum canis) and bacteria (Escherichia coli, Salmonella enteriditis, Staphylococcus aureus and Bacillus subtilis). M. canis showed dose-dependent susceptibility only towards ethanol leaf and bark extracts. C. albicans, were resistant to all types of plant extracts. Results were statistically smaller to antifungal drug ketoconazole and miconazole at equivalent concentration. Both ethanol and aqueous extracts of Cassia auriculata leaves and barks exhibit antibacterial activity against S. aureus and only the ethanol extracts of leaf and bark were detected against Bacillus subtilis. The results were compared to antibacterial drugs chloramphenicol, ampicillin, penicillin G, and enrofloxacin. The antibacterial activity was statistically similar to penicillin G. Based on the current findings, it can be concluded that this plant has antimicrobial activity, which is as potent as standard antimicrobial drugs.

• International Journal of Oral Biology
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• v.33 no.4
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• pp.197-203
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• 2008

It has been reported that the antimicrobial susceptibility patterns of viridans streptococci vary according to geographical region. Although several studies on the antibiotic resistance of viridans streptococci in foreign countries have been reported, little is known about the distribution of resistance among viridans streptococci in Korea. In this study, 88 isolates of viridans streptococci from Korean students' dental plaque were identified as 12 different species. The susceptibility of these isolates to 8 antibiotics was investigated. The in vitro antibiotic activity of penicillin G, ampicillin, vancomycin, streptomycin, gentamicin, erythromycin, amoxicillin, and tetracycline was measured by the broth microdilution method. The range of the minimum inhibitory concentrations (MIC), MIC50, MIC90, and the percentage of the susceptible isolates were determined. Streptococcus mutans and Streptococcus salivarius were susceptible to the 8 antibiotics. Isolates with resistance to vancomycin, streptomycin, and amoxicillin were not found. The overall resistance rates of the 88 isolates to penicillin G, ampicillin, gentamicin, erythromycin, and tetracycline were 12.5%, 62.5%, 62.5%, 26.1%, and 26.1%, respectively.

• Microbiology and Biotechnology Letters
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• v.43 no.1
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• pp.84-90
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• 2015

Seventeen ampicillin-sensitive coagulase-negative staphylococci (CNS) isolates identified in jeotgal were subjected to assessments for antibiotic susceptibility and safety hazards. Fifteen of the 17 CNS strains exhibited phenotypic resistances to at least one antibiotic, and their prevailing resistance was to penicillin G. The dfrA gene for trimethoprim and tetK for tetracycline were amplified by PCR from the two strains, respectively. α-Hemolytic activity was not detected from the 17 strains, while five strains presented δ-hemolytic activity. Among the five strains, two strains exhibited β-hemolytic activity. Biofilm was formed from twelve strains. All of the tested phenotypic characteristics were expressed in a strain-specific manner.

• Korean Journal of Veterinary Service
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• v.22 no.2
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• pp.121-128
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• 1999

The present study was conducted to investigate the incidence of pneumonic lesions with special regard to enzootic pneumonia and the microbiology of pneumoic lungs from 544 slaughter pigs during the period from October 1995 to September 1996. The incidence of enzootic pneumonic lesion was 76.3% (41s/s44) and pleurisy was detected from 7.9% of slaughter pigs. Seasonal prevalence of pneumonic lesions in slaughter pigs were in order of prevalence of 82.9% in spring, 76.8% in winter, 74.8% in autumn and 69.0% in summer, respectively. Pasteurella multocida, Streptococcus sp, Str suis, Corynebacterium sp, Actinobacillus pleuropneumoniae, Hemophilus parasuis, and Klebsiella pneumoniae were detected in order of prevalence from 16.9%, 15.9%, 7.5%, 6.0%, 1.4%, 1.0% and 0.5% of 415 pneumonic lungs, respectively. P multocida were susceptible to oxytetracycline, polymyxin-B, streptomycin, and vancomycin, while the majority of them were resistant to amoxicillin, ampicillin, cephalothin, kanamycin, and penicillin-G. Str suis were susceptible to amoxicillin, ampicillin, cephalothin, penicillin-G, although the majority of them were resistant to erythromycin, oxytetracycline, streptomycin, vancomycin. A pleuropneumoniae were susceptible to ampicillin, and cephalothin, but the majority of them were resistant to oxytetracycline.

• Korean Journal of Microbiology
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• v.23 no.4
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• pp.309-314
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• 1985

Autoxotrophic mutants of a cellulolytic baterium Cellulomonas sp. CS 1-1 were grown at $30^{\circ}C$ for 6hr using a complete medium containing 0.5M sucrose and for another 90 min after addition of 0.3 U/ml penicillin G, and were protoplasted by 0.2mg/ml lysozyme for 2hr. Prototrophic recombinants were obtained at the rates of $10^{-3}$ to $10^{-5}$by fusing the protoplasts in the presence of 40% polyethyleneglycol3350. Nystatin could be used to eliminate fungal contamination during the regeneration of the plotaplasts.

• Korean Journal of Microbiology
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• v.23 no.4
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• pp.265-270
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• 1985

Overall procedure of cell fusion between Brevibacterium flavum and Corynebacterium glutamicum was morphologically observed by transmission electron microscopy. Protoplasts formed by treatment of cells with penicillin G and lysozyme in order were released through the pores generated on a certain region of cell walls to be spherical form. When two different protoplasts were met, cell wall and membrane in the contact zone was disappeared and followed by the mutual exchange of cytoplasmic and/or chromosomal materials. Cell xall regeneration speed of the protoplasts fused was slower than that of the non-fused, whereas the size of the former was confirmed as bigger than that of the latter.

• Microbiology and Biotechnology Letters
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• v.3 no.3
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• pp.147-156
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• 1975

The possibility of using tapioca pellets as a raw material in glutanmic acid fermentation by Microcuccus glutamicus is shown. The ground pellets were diluted with water to 20％ solid level and treated with $\alpha$-anylase prepared from a thermophilic Actinomycetes strain culture for 90 min at 85$^{\circ}C$ under pH 6.0. The liquefied solution was further saccharified with commercial glucoamylase for 36 hours under the reaction conditions of 55$^{\circ}C$ and pH 5.0. The inhibitory effect of excess biotin content, 16 $\mu\textrm{g}$ Per liter of the hydrolzate, could be reduced effectively by adding 10 IU of penicillin per ml of the medium after five hours of the fermentation. The maximum glutamic acid yield of 38.5 g/l was obtained after 60 hours of shaking culture at 28-3$0^{\circ}C$.

• Microbiology and Biotechnology Letters
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• v.23 no.5
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• pp.556-558
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• 1995

A strain which showed cephalosporin C resistance and 7-aminocephalosporanic acid sensitivity was isolated from nature. Among the isolates, SS5 was sensitive to cephalosporin C, penicillin G, ampicillin, 7-aminocephalosporanic acid, 6-aminopenicillanic acid, and 7-aminodeacetoxy cephatosporanic acid at concentrations of 1,000 $\mu $g/ml, 2,000 $\mu $g/ml, 3,000 $\mu $g/ml, 30 $\mu $g/ml 100 $\mu $g/ml and 100 $\mu $g/ml, respectively. But SS5 was sensitive at very low concentration of chloramphenicol, kanamycin, neomycin, streptomycin and tetracycline. Since SS5 was sensitive to 7-ACA (30 $\mu $g/ml) and didn't have $\beta $-lactamase activity on the cephalosporin C, SS5 could be useful as an indicator strain for the production of 7-ACA, which is an important precursor for the synthesis of many semisynthetic cephalosporins.

• Korean Journal of Microbiology
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• v.28 no.2
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• pp.98-103
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• 1990

Brevibacterium lactofermentum SWA (arg trp) and B. lactofermentum SWB (met ser) were obtained from UV and NTG treatment. The rates of protoplast formation by B. lactofermentum SWA and SWB were 99.93% and 99.98%, respectively when each strain was treated with penicillin G in mid exponential growth phase, followed by incubation with 400 $\mu\textrm{g}$/ml of lysozyme in lysis fluid supplemented with 0.4M sucrose. Frequencies of protoplast regeneration in B. lactofermentum SWA and B. lactofermentum SWB were 9.27% and 10.32% respectively, on regeneration medium containing 0.5M sodium succinate, 50 mM $Mg^{2+}$, and 3% PVP. In intraspecific protoplast fusion between B. lactofermentum SWA and B. lactofermentum SWB, fusion frequency of $2.30\times 10^{-5}$ was observed by using the 100mM $CaCl_{2}$ and 30% PEG 6,000 in fusion fluid. Relative recombinant frequencies in each marker by means of selective media could be used for genetic analysis.