• 미생물학회지
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• 제32권1호
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• pp.40-46
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• 1994

Polychlorinaed biphenyls(PCBs) 와 biphenyl을 분해하는 Pseudomonas sp. DJ-12에서는 그 초기 분해과정에 pcb ABCD 유전자들이 관여하고 있음이 밝혀졌다. 그 중 pcbCD와 pcdD 유전자를 E. coli XL1-Blue에 클로닝하여 E. coli CU103 과 CU105 균주를 각각 제조하였다. E. coli CU103은 2,3-dehydroxybuphenyl dioxygenase(2,3-DHBP)와 meta-cleavage compound(MCP) hydrolase를 생성하여 2,3-dihydroxybiphenyl을 benzoate로 변환시켜 주었다. E. coli CU1 과 CU103 에서 pcbC 유전자의 산물인 2,3-DHBP dioxygense의 활성도는 Pseudomonas sp. DJ-12에서 보다 약 17배 높았으며, E. coli CU105에서 pcbD의 산물인 MCP hydrolase는 약 3배 더 높게 나타났다.

• 미생물학회지
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• 제40권2호
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• pp.121-126
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• 2004

4-chlorobiphenyl (4CB)의 분해균주인 Pseudomonas sp. strain DJ-12의 16S rDNA의 염기서열을 분석한 결과 Comamonas sp. strain DJ-12로 재분류 되었다. Pseudomonas sp. strain DJ-12로부터 4CB의 분해결과 생성되는 2,3-dihydroxybiphenyl을 계속 분해하는데 관여하는 pcbC1Dl 유전자를 이미 보고된 바 있다. 이번 연구에서는 Comamonas sp. strain DJ-12로부터 4CB 분해에 관여하는 pcbABC2D2 유전자를 클로닝하여 염기서열을 분석하였다. PcbAB 및 pcbCD 유전자들의 염기서열은 48, 65％, 추정 아미노산 서열은 33, 42％의 낮은 유사도를 보였다. 본 연구에서 얻어진 pcbC2D2 유전자는 이미 보고만 pcbCIDl 유전자와 염기의 개수와 서열의 유사도가 서로 다름을 보여 주었다. Comamonas sp. strain DJ-12의 두 가지 pcbCD유전자들은 Southern hybridization 결과에서도 유사성을 보이지 않았으며, 서로 다른 위치에 존재함을 보여주었다. 그러나 2,3-dihydroxybiphenyl의 분해 특성은 동일하였다. 이와 같은 결과는 Comamonas sp. strain DJ-12 균주가 2조의 pcbCD 유전자를 가지고 있다는 것을 의미하는 것이다.

• Journal of Microbiology
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• 제34권4호
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• pp.349-354
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• 1996

Pseudomonas sp. P20 is a natural isolate which is capable of degrading biphenyl and 4-chlorobiphenyl. From a clone of pCK1022 harboring pcbCD genes of Pseudomonas sp P20, a pcbD gene encoding 2-hydroxy-6-oxo-6-phenylhexa-2, 4-dienoic acid (HOPDA) hydrolase was subcloned in Escherichia coli XL-1-Blue by using pBluescript SK(+) vector. The 2.8-kb HindII fragment harboring the pcbD gene cloned in pCK 1024 had a single site for each of XhoI, SalI, BstXI, and XbaI restriction enzymes. Escherichia coli CK1024 had a single site for each of XhoI, SalI, BstXI, and XbaI restriction enzymes. Escherichia coli CK1024 carrying pCK0124 degraded HOPDA to benzoate and 2-hydroxypenta-2, 4-dienoate by HOPDA hydrolase encoded by pcbD gene as effectively as E coli CK 1022 HARBORING pcbCD genes.

• 생명과학회지
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• 제20권8호
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• pp.1207-1213
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• 2010

본 연구의 목적은 랫드의 두개결손부 모델에서 돼지 해면질골을 지지체로 사용했을 때의 효과를 평가해보고자 하였다. 임계결손부의 형성은 30마리의 수컷 Sprague-Dawley 랫드에서 실시하였으며 동물들은 임계결손군(CD group, n=10), 베타 삼인산칼슘군(BT group, n=10) 및 돼지 해면질골군(PCB group, n=10)으로 나누었다. 각각의 결손부위는 피브린 글루와 혼합시킨 베타 삼인산칼슘 또는 돼지 해면질골로 채워졌으며 CD군은 결손부위에 이식재를 이식하지 않았다. 모든 랫드들은 골이식 수술 8주 후에 희생되었으며 희생 후 육안검사, 단순 방사선촬영, micro-CT 촬영 및 조직검사를 통해 골형성 정도를 평가하였다. 결과에서 골결손부의 치유는 CD군에서 가장 낮았으며 PCB군에서는 유의성 있는 새로운 골형성을 확인할 수 있었다. 또한 방사선촬영 결과, 조직학적 평가 및 micro CT 촬영 결과에서 골이식 시 돼지 해면질골이 베타 삼인산칼슘보다 새로운 골형성에 있어 더욱 효과적임을 관찰할 수 있었다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 1994년도 한국생물과학협회 학술발표대회
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• pp.120.1-120
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• 1994

• 한국미생물·생명공학회지
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• 제22권4호
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• pp.353-359
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• 1994

Pseudomonas sp. P20 was a bacterial isolate which has the ability to degrade 4-chlorobi- phenyl(4CB) to 4-chlorobenzoic acid via the process of meta-cleavage. The recombinant plasmid pCK1 was constructed by insetting the 14-kb EcoRI fragment of the chromosomal DNA containing the 4CB-degrading genes into the vector pBluescript SK(+). Subsequently, E. coli XL1-Blue was transformed with the hybrid plasmid producing the recombinant E. coli CK1. The recombinant cells degraded 4CB and 2,3-dihydroxybiphenyl(2,3-DHBP) by the pcbAB and pcbCD gene products, respectively. The pcbC gene was expressed most abundantly at the late exponential phase in E. coli CK1 as well as in Pseudomonas sp. P20, and the level of the pcbC gene product, 2,3-DHBP dioxygenase, expressed in E. coli CK1 was about two-times higher than in Pseudomonas sp. P20. The activities of 2,3-DHBP dioxygenase on catechol and 3-methylcatechol were about 26 to 31% of its activity on 2,3-DHBP, but the enzyme did not reveal any activities on 4-methylcatechol and 4-chlorocatechol.

• 한국미생물·생명공학회지
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• 제24권6호
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• pp.657-663
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• 1996

Pseudomonas sp. P20 isolated from the polluted environment is capable of degrading biphenyl and 4-chlorobiphenyl. The pcbABCD genes responsible for degradation of biphenyl and 4-chlorobiphenyl were cloned using pBluescript SK(+) from the chromosomal DNA of Pseudomonas sp. P20 to construct pCK1 and pCK102, harbouring pcbABCD and pcbCD, respectively. The 2, 3-DHBP dioxygenase gene, pcbC, was cloned again from pCK102 by using pKT230 which is known as a shuttle vector and pKK1 hybrid plasmid was constructed. The E. coli KK1 transformant obtained by transforming the pKK1 into E. coli XL1-Blue showed 2, 3-DHBP dioxygenase activity. The specific 2, 3-DHBP dioxygenase activity of E. coli KK1 was similar to that of the E. coli CK102, but much higher than those of the natural isolates, Pseudomonas sp. DJ-12 and Pseudomonas sp. P20.

• 한국환경과학회지
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• 제7권4호
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• pp.565-572
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• 1998

This study estimate the degree of contamination in the streamwater around the Sudokwon landfill site. It was sampled at 10 sites in Jan., Apr., Jul. and Oct., 1996. There were analyzed five kinds of toxic chemical material-CN, AES, PCB, As, Org-p, and four kinds of heavy metal-Pb, Hg, Cd, $C^{+6}$ The result are 1)The COD was generally increased to compare before landfall, 2) The Org-P and PCB were not detected at all points, 3) The concentrations of Pb, $C^{+6}$ and As were lower than the environmental criteria values, 4) The CN, Hg and Cd were over envirommental criteria values. and so emergent regulation is needed, 5) The effects of the streamwater contamination were not only the leachate of the landfall, but also the small factories and agricultural land around the landfill.

• 한국통신학회논문지
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• 제27권12C호
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• pp.1282-1287
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• 2002

본 논문에서는 가정 내의 전화선을 이용한 홈 네트워크 기술인 10Mbps HomePNA(Home Phoneline Networking Alliance) 20 PHY 회로의 설계 및 그에 대한 검증방법을 보여 준다. HomePNA 2.0 PHY 회로는 MII(Media Independent Interface)와 AFE 인터페이스에 의해 외부와 연결된다. 설계된 10Mbps HomePNA 2.0 PHY의 회로의 전체 구조는 Management block IEEE 802.3 CSMA/CD MAC(Media Access Control) block, 변조 및 복조 block으로 크게 구성된다. 설계된 회로는 프로토타입 FPGA PCB 보드를 이용하여 검증하였다. 또한, Linux 기반의 드라이버 프로그램을 개발하여 HomePNA 프레임 데이터 전송의 기본적인 동작을 확인하였으며, HomePNA 2.0 링크 계층 프로토콜의 RNCF(Rate Negotiation Control Function)에 의하여 전송속도의 변화를 확인하였다.

• 한국환경과학회지
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• 제14권6호
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• pp.583-596
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• 2005

Samples of water, soil, and sediment were taken from 10 streams of Andong city in Gyeongbuk province in October 2004. To assess the degree of environmental pollution for each stream site, the chemical analyses of pollutants such as T-N, T-P, COD, heavy metal, organophosphorous and organochlorine pesticides, and dioxin-like PCB congeners were implemented using the standard process tests or the U. S. EPA methods. In addition, biological assessment using insect immune biomarkers was conducted on the same environmental samples to complement the chemical assessment. Except Waya stream (T-N; 2.91 mg/L, T-P; 0.16 mg/L, COD; 14.0 mg/L) with above the environmental quality standards, the T-P and COD concentrations of 9 sites are relatively low. The contents of Pb and Cd in samples taken from each stream were much lower than environmental quality standards. However, in comparison with soil samples of other streams, several times higher concentrations of Pb and Cd were found in locations at Mi, Gilan, Yeonha, and Waya stream sites. Dementon-S-methyl, diazinon, parathion, and phenthoate compounds among organophosphorous pesticides were detected as concentrations of ppb levels, respectively, from soil samples collected in the vicinity of Gilan, Mi, Norim, and Waya stream. On the other hand, 16 organochlorine pesticides and 12 dioxin-like PCB congeners selected in this study were not found in all samples. In particular, considering significant disrupting effects of Waya stream's samples on insect immune capacity, this stream seems to be contaminated with investigated and/or univestigated pollutants in this study.