• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.163-170
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• 2002

This report describes the use of a transtubular bioreactor to study the relative effects of diffusion versus perfusion of medium on antibody production by a hybridoma cell line. The study was performed with a high-density cell culture maintained in a serum-free, low-protein medium for 77 days. It was determined that the reactor possessed a macro-mixing pattern residence time distribution similar to a continuous stirred tank reactor (CSTR), However, due to the arrangement of the medium lines in the reactor, the flow patterns for nutrient distribution consist of largely independent medium path lengths ranging from short to long. When operated with cyclic, reversing, transtubular medium flow, some regions of the reactor (with short residence times) are more accessible to medium than others (with long residence times). From this standpoint, the reactor can be divided into three regions: a captive volume, which consists of medium primarily delivered via diffusion; a lapped volume, which provides nutrients through unilateral convection; and a swept volume, which operates through bilateral convection. The relative sizes of these three volumes were modified experimentally by changing the period over which the direction of medium flow was reversed from 15 min (larger captive volume) to 9 h (larger swept volume). The results suggest that antibody concentration increases as the size of the diffusion-limited (captive) volume is increased to a maximum at around 30 min with a sharp decrease thereafter. As reflected by changes in measured consumption of glucose and production of lactate, no significant difference in cellular metabolism occurred as the reactor was moved between these different states. These results indicate that the mode of operation of the transtubular bioreactor may influence antibody productivity under serum-free, low-protein conditions with minimal effects on cellular metabolism.

• Smart Structures and Systems
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• v.8 no.3
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• pp.303-320
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• 2011

Comparing to active damage monitoring, impact localization on composite by using time reversal focusing method has several difficulties. First, the transfer function of the actuator-sensor path is difficult to be obtained because of the limitation that no impact experiment is permitted to perform on the real structure and the difficulty to model it because the performance of real aircraft composite is much more complicated comparing to metal structure. Second, the position of impact is unknown and can not be controlled as the excitation signal used in the active monitoring. This makes it not applicable to compare the difference between the excitation and the focused signal. Another difficulty is that impact signal is frequency broadband, giving rise to the difficulty to process virtual synthesis because of the highly dispersion nature of frequency broadband Lamb wave in plate-like structure. Aiming at developing a practical method for on-line localization of impact on aircraft composite structure which can take advantage of time reversal focusing and does not rely on the transfer function, a PZT sensor array based phase synthesis time reversal impact imaging method is proposed. The complex Shannon wavelet transform is presented to extract the frequency narrow-band signals from the impact responded signals of PZT sensors. A phase synthesis process of the frequency narrow-band signals is implemented to search the time reversal focusing position on the structure which represents the impact position. Evaluation experiments on a carbon fiber composite structure show that the proposed method realizes the impact imaging and localization with an error less than 1.5 cm. Discussion of the influence of velocity errors and measurement noise is also given in detail.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.23 no.3
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• pp.328-334
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• 2012

This paper proposes a design technique of wide-band antenna for handy phone with 700 MHz LTE(Long Term Evolution) low frequency bandwidth. The proposed antenna based on the PIFA(Planar Inverted-F type Antenna) structure was designed and considered. In order to realize the wide bandwidth, a round structure which is able to control the electric path length of current and a branch line element which can be obtain the dual resonance characteristics were introduced in this design. As a result, It was realized about 95 MHz bandwidth in spite of very small space of $30{\times}34mm$ used for FR-4 substrate with relative permitivity of 4.4 at 700 MHz band. Measurement results of return loss, bandwidth and gain radiation pattern were agreed well with their calculation results.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.15 no.1
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• pp.291-298
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• 2015

It is pretty hard to sell the kids' furniture online because of the characteristics of the consumers that they will like to make purchases after looking at the actual objects and then considering purchasing them. In this paper, we made the scheme to boost the on-line malls of the kids' furniture market by collecting the consumers' intention to buy before purchasing with questionnaire survey, comparing it with the real data of purchasing, and analyzing the differences. We analyzed the visit number of shopping mall, the page number of views, page duration time per visit, and customer's shopping path by using log data from the children's furniture online shopping mall server. In addition, we analyzed where the customer's influx route is among online advertising. We found that there are requirements for the designs, prices, brand names, and practicalities by the consumers purchasing the kids' furniture online. To strengthen competitiveness of the online kids' furniture malls, we proposed that reinforcing the on-line experiences of furniture, the differential strategies for the products, ensuring the fixed customers are needed.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.5 s.335
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• pp.23-28
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• 2005

Recently, new concept, NP(Network Processor) was emerged into communication systems to cope with the various service requirements from Internet users. NP is an unique promising technique to capable of implementing of the packet processing in wire-speed and providing the flexibility for supporting the newly network services, having satisfied with implementation using hardware and software respectively in past, This paper deals with the implementation techniques and evaluation results of the line card capable to do packet forwarding function with packet processing power of wire-speed and applicable to various physical interfaces. There are several interfaces of POS, Gigabit ethernet and EPON in E-OLT(EPON Optical Line Terminal) system of PATH(Photonic Access To Home) network. Therefore, the E-OLT's packet forwarding engine have to support various subscriber's interface in wire speed. Our system is implemented the subscriber's card in daughter board and the setup procedure is done by system firmware based on the module's identifier acquired from installed physical board.

• Journal of the Korean GEO-environmental Society
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• v.9 no.6
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• pp.61-70
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• 2008

In this study, undrained triaxial (CU) tests were performed on low-plastic silt of Nakdong River in order to investigate the undrained shear behavior of low-plastic silt. In experimental results, the deviator stress showed the hardening behavior after reaching its yield stress like the tendency of common sand, and the pore water pressure was gradually decreased to critical state after the maximum value. In the effective stress paths, regardless of consolidation stress or overconsolidation ratios, both a critical state line (CSL) and a phase transformation line (PTL) exist in the effective stress path that is similar to the case of sand. The behavior of low-plastic silt was predicted by the Modified Cam-Clay (MCC) model, the Jordan and the Elman-jordan model that is artificial neural network model. According to predicted results, the overall undrained shear behavior of low-plastic silt could not be predicted with the MCC model, but the Jordan and Elman-Jordan model showed well-matched experiment results.

• Journal of Welding and Joining
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• v.27 no.2
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• pp.32-37
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• 2009

In shipyard, plate forming is widely used to form the ship hull plate in various shapes. Line heating method by using a flame torch is one of the major shipbuilding processes carried out by skilled workers. Since the forming characteristics depend upon their experiences in manual forming, there are much variations between products and difficulties in communication between engineers and workers. Hence, it needs to develop an automatic forming system which can not only reduce the working time and rework costs but also improve the working environment and hull forming productivity. One of the final goals of plate forming automation is to form a target shape from the initial plate automatically. For automated plate forming, it is required to determine where and how to heat on the plate. To realize this procedure, the inverse problem should be first solved and the effect of curvature shape formed at the heating path should be investigated. In this study, the inverse problem was solved by geometrical approach using the relationship between bending angle and radius of curvature of the curved shape. In addition, experiments of two-dimensional plate forming were performed with the distance-based method considering the curved bending with curvature. The result of the formed shape agreed considerably well with the target shape.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.38 no.3
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• pp.228-235
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• 2010

This paper deals with an autonomous flight controller design problem for a tilt-rotor aircraft in rotary-wing mode. The inner-loop algorithm is designed using the output-based approximate feedback linearization. The model error originated from the feedback linearization is cancelled within allowable tolerance by using single-hidden-layer neural network. According to Lyapunov direct stability theory, the adaptive update law is derived to run the neural network on-line, which is based on the linear observer dynamics. Moreover, the outer-loop algorithm is designed to track the trajectory generated from way-point guidance. Especially, heading and flight-path angle line-of-sight guidance are applied to the outer-loop to improve accuracy of the landing tracking performance. The 6-DOF nonlinear simulation shows that the overall performance of the flight control algorithm is satisfactory even though the collective input response shows instantaneous actuator saturation for a short time due to the lack of the neural network and the saturation protection logic in that loop.

• The Journal of the Korea institute of electronic communication sciences
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• v.9 no.12
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• pp.1337-1344
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• 2014

The objective of an echo canceller connected to any end of a communication line such as digital subscriber line (DSL) is to compensate the outgoing transmit signal in the receiving path that the hybrid circuit leaks. The echo canceller working in a full duplex environment is an adaptive system driven by the local signal. Conventional echo canceller that implement the least mean square (LMS) algorithm provides a low computational burden but poor convergence properties. The length of the echo canceller will directly affect both the degree of performance and the convergence speed of the adaptation process. To cancel long time-varying echoes, the number of tap coefficients of a conventional echo canceller must be large, which decreases the convergence speed of the adaptive filter. This paper proposes an alternative technique for the echo cancellation in a telecommunication channel. The new technique employs the bi-directional least mean square (LMS) algorithm for adaptively computing the optimal set of the coefficients of the echo canceller, which is composed of weighted combination of both feedforward and feedback algorithms. Finally, Simulation results as well as mathematical analysis demonstrates that the proposed echo canceller has faster convergence speed than the conventional LMS echo canceller with nearly equivalent complexity of computation.

• Journal of the Korean Institute of Intelligent Systems
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• v.14 no.7
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• pp.907-913
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• 2004

The major interest of general mobile robot is making a route and following a maked route. But, In the case of robot that is in need of movement of partial high speed, the condition of dynamic limitation is exist, and in these conditions, it demands controlling against movements we want. In this paper, in respect of the following a route at the situation that don't have the environmental map, that is, unknown environments, to prevent the slide of moving robot or the overturn that can happen for it moves fast, we organize the dynamic condition of limitation using the fuzzy logic, and we obtain more safe and fast route tracing ability by changing the standard velocity. Especially, by modeling the line tracing mobile robot, we design the tracing controller against a realtime changing target, and using the fuzzy optimized velocity limitation controller, we confirm that our robot shows its stable tracing ability by limiting its velocity intelligently against the continuously changing line.