• Journal of Microbiology and Biotechnology
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• v.13 no.6
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• pp.846-852
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• 2003

rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level.

• Asian Journal of Atmospheric Environment
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• v.5 no.3
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• pp.172-180
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• 2011

The transfer of microorganisms is important process for ecosystems. Microorganisms in dryland can transport itself to wetland through atmospheric diffusion, but only few papers reported about the atmospheric bioaerosol present over dryland. We carried out the direct sampling using a tethered balloon over Dunhuang City, China's northwestern dryland. Bioaerosols were collected using a tethered balloon with a bioaerosol collector at 820 m above the ground (1,960 m above the sea level) around noon on August 17, 2007. The bioaerosols were cultured after the collection at Dunhuang Meteorological observatory. Two strains of molds were isolated using the Nutrient agar medium. About 400-bp 18S rRNA partial sequences were amplified by PCR and determined afterwards. The results of a homology search by 18S rRNA sequences of isolates in DNA databases (GenBank, DDBJ, and EMBL) and an observation of the form revealed that two bioaerosols in the convective mixed layer over Dunhuang City were Cladosporium sp. and Aspergillus sp.

• Microbiology and Biotechnology Letters
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• v.8 no.2
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• pp.125-133
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• 1980

A microorganism which produced D-glucose isomerase was identified to be similar to Streptomyces antibioticus on the morphological, cultural and physiological characteristics except the spore chain and the utilization of sucrose. D-xylose grown cells of Streptomyces sp. strain K-17 were disrupted by grinding with sea sand. D-glucose isomerase was partially purified with the fractionation by ammonium sulfate, Mn-treatment, DEAE-cellulose column chromatography, DEAE-sephadex (A-50) column chromatography and gel filtration of sephadex G-200. The enzyme was purified about 380 fold with 25 ％ recovery.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.195-200
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• 2000

Bacillus cereus BS229 was identified as a bacteriocin producer with a bactericidal activity against Bacillus thuringiensis subsp. Thomsoni BR-40. Bacillus cereus BS229 and cerein BS229, named tentatively as the bacteriocin produced by Bacillus cereus BS229, showed a narrow spectrum of actibity against Gram-positive and Gram-negative bacteria, along with yeast and molds. Production of cerein BS229 in a 5-1 fermenter followed typical kinetics of primary metabolite synthesis. The antibacterial activity of cerein BS229 on sensitive indicator cells disappeared completely by ${\alpha}-chmotrypsin$ or proteinase K, which indicates its proteinaceous nature. Cerein BS229 seemed to be very stable throughout the pH range of 2.0 of 9.0 and it was relatively heat labile, despite the fact that bacteriocin activity was still detected after being boied for 30min. Cerein BS229 actibity has been changed with some of the organic solvents such as toluene, ethanol, and chloroform. Direct detection of cerein BS229 actibity on SDS-PAGE suggested that it had an apparent molecular mass of about 8.2 kDa.

• Proceedings of the KIEE Conference
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• 1998.11c
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• pp.941-943
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• 1998

Exploitation of equipment with cross linked polyethylene (XLPE) insulation requires its condition monitoring and diagnostic. Traditionally diagnostics of insulation is carried out by means of partial discharge detection. Many researchers have developed a lot of methods to identify the defect by the PD form. However, such identification of a defect, for example, void, inclusion or treeing, does not say about its danger from a point of view of full insulation gap breakdown and insulation construction failure. The information about the form and size of formed upon high voltage treeing is necessary for prediction of the remained resource of XLPE insulation. For this purpose we carry out experimental research for determination of the dependencies between PD characteristics in XLPE upon time and three dimension PD patterns of corresponding treeing. The investigations were carried out by means of electrical measurement of PD current and simultaneous optical recording of treeing image. Test results show that the PD patterns can be applied for detecting tree growth well.

• Journal of applied mathematics & informatics
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• v.5 no.2
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• pp.373-382
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• 1998

Most of the works in Time Series Analysis are based on the Auto Regressive Integrated Moving Average (ARIMA) models presented by Box and Jeckins(1976). If the data exhibits no ap-parent deviation from stationarity and if it has rapidly decreasing autocorrelation function then a suitable ARIMA(p,q) model is fit to the given data. Selection of the orders of p and q is one of the crucial steps in Time Series Analysis. Most of the methods to determine p and q are based on the autocorrelation function and partial autocor-relation function as suggested by Box and Jenkins (1976). many new techniques have emerged in the literature and it is found that most of them are over very little use in determining the orders of p and q when both of them are non-zero. The Durbin-Levinson algorithm and Innovation algorithm (Brockwell and Davis 1987) are used as recur-sive methods for computing best linear predictors in an ARMA(p,q)model. These algorithms are modified to yield an effective method for ARMA model identification so that the values of order p and q can be determined from them. The new method is developed and its validity and usefulness is illustrated by many theoretical examples. This method can also be applied to an real world data.

• Parasites, Hosts and Diseases
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• v.52 no.6
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• pp.701-705
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• 2014

The rumen parasite, Gastrothylax crumenifer (Platyhelminthes: Gastrothylacidae), is a highly pathogenic trematode parasite of goat (Capra hircus). It sucks blood that causes acute disease like anemia, and severe economic losses occur due to morbidity and mortality of the ruminant infected by these worms. The study of these rumen paramphistomes, their infection, and public health importance remains unclear in India especially in the western part of state Uttar Pradesh (U.P.), Meerut, India, where the goat meat consumption is very high. This paper provides the molecular characterization of G. crumenifer recovered from the rumen of Capra hircus from Meerut, U.P., India by the partial sequence of 28S rDNA. Nucleotide sequence similarity searching on BLAST of 28S rDNA from parasites showed the highest identity with those of G. crumenifer from the same host Capra hircus. This is the first report of molecular identification of G. crumenifer from this part of India.

• Parasites, Hosts and Diseases
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• v.55 no.3
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• pp.327-331
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• 2017

A fly larva was recovered from a boil-like lesion on the left leg of a 33-year-old male on 21 November 2016. He has worked in an endemic area of myiasis, Uganda, for 8 months and returned to Korea on 11 November 2016. The larva was identified as Cordylobia anthropophaga by morphological features, including the body shape, size, anterior end, posterior spiracles, and pattern of spines on the body. Subsequent 28S rRNA gene sequencing showed 99.9% similarity (916/917 bp) with the partial 28S rRNA gene of C. anthropophaga. This is the first imported case of furuncular myiasis caused by C. anthropophaga in a Korean overseas traveler.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.31 no.1 s.256
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• pp.43-49
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• 2007

A new electric coil design methodology using the notion of topology optimization is developed. The specific design problem in consideration is to find optimal coil configuration that maximizes the Lorentz force under given magnetic field. Topology optimization is usually formulated using the finite element method, but the novel feature of this method is that no such partial differential equation solver is employed during the whole optimization process. The proposed methodology allows the determination of not only coil shape but also the number of coil turns which is not possible to determine by any existing topology optimization concept and to perform single coil strand identification algorithm. The specific applications are made in the design of two-dimensional fine-pattern focusing coils of an optical pickup actuator. In this method, the concept of equivalent magnetizing current is utilized to calculate the Lorentz force, and the optimal coil configuration is obtained without any initial layout. The method is capable of generating the location and shape of turns of coil. To confirm the effectiveness of the proposed method in optical pickup applications, design problems involving multipolar permanent magnets are considered.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.8
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• pp.3270-3294
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• 2020

Some things come easily to humans, one of them is the ability to navigate around. This capability of navigation suffers significantly in case of partial or complete blindness, restricting life activity. Advances in the technological landscape have given way to new solutions aiding navigation for the visually impaired. In this paper, we analyze the existing works and identify the challenges of path selection, context awareness, obstacle detection/identification and integration of visual and nonvisual information associated with real-time assisted mobility. In the process, we explore machine learning approaches for robotic path planning, multi constrained optimal path computation and sensor based wearable assistive devices for the visually impaired. It is observed that the solution to problem is complex and computationally intensive and significant effort is required towards the development of richer and comfortable paths for safe and smooth navigation of visually impaired people. We cannot overlook to explore more effective strategies of acquiring surrounding information towards autonomous mobility.