Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Differentiation of Actinomycete Genera Based on Partial rpoB Gene Sequences

  • Kim, Bum-Joon (Department of Microbiology, College of Medicine, Seoul National University) ;
  • Koh, Young-Hwan (Department of Food Science and Engineering, Cheju National University) ;
  • Chun, Jong-Sik (School of Biological Sciences, Seoul National University) ;
  • Kim, Chang-Jin (Department of Microbiology, College of Medicine, Konkuk University) ;
  • Lee, Seung-Hyun (Korea Research Institute of Bioscience and Biotechnology) ;
  • Cho, Moon-Jae (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Hyun, Jin-Won (Department of Biochemistry, College of Medicine, Cheju National University) ;
  • Lee, Keun-Hwa (Department of Microbiology, College of Medicine, Seoul National University) ;
  • Cha, Chang-Yong (Department of Microbiology, College of Medicine, Seoul National University) ;
  • Kook, Yoon-Hoh (Department of Microbiology, College of Medicine, Seoul National University)
  • Published : 2003.12.01
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Abstract

rpoB DNAs (279 bp) from 34 species of 5 actinomycete genera were sequenced and a phylogenetic tree was constructed based on the sequences obtained. The genera were clearly differentiated in the rpoB tree, forming clades specific to their respective genus. In addition, 2 signature amino acid residues specific to Streptomyces were found in a multiple alignment of the deduced amino acid sequences. To empirically confirm that this rpoB gene analysis system could be used to differentiate actinomycete isolates, the proposed system was used to identify 16 actinomycete isolates from Jeju Island. All isolates were successfully differentiated into the genera Streptomyces and Micromonospora. Accordingly, this is the first report that an rpoB sequence analysis has been effectively used to differentiate actinomycete strains at the genus level.

Keywords

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