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Global Transcriptome-Wide Association Studies (TWAS) Reveal a Gene Regulation Network of Eating and Cooking Quality Traits in Rice

  • Weiguo Zhao;Qiang He;Kyu-Won Kim;Feifei Xu;Thant Zin Maung;Aueangporn Somsri;Min-Young Yoon;Sang-Beom Lee;Seung-Hyun Kim;Joohyun Lee;Soon-Wook Kwon;Gang-Seob Lee;Bhagwat Nawade;Sang-Ho Chu;Wondo Lee;Yoo-Hyun Cho;Chang-Yong Lee;Ill-Min Chung;Jong-Seong Jeon;Yong-Jin Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.207-207
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    • 2022
  • Eating and cooking quality (ECQ) is one of the most complex quantitative traits in rice. The understanding of genetic regulation of transcript expression levels attributing to phenotypic variation in ECQ traits is limited. We integrated whole-genome resequencing, transcriptome, and phenotypic variation data from 84 Japonica accessions to build a transcriptome-wide association study (TWAS) based regulatory network. All ECQ traits showed a large phenotypic variation and significant phenotypic correlations among the traits. TWAS analysis identified a total of 285 transcripts significantly associated with six ECQ traits. Genome-wide mapping of ECQ-associated transcripts revealed 66,905 quantitative expression traits (eQTLs), including 21,747 local eQTLs, and 45,158 trans-eQTLs, regulating the expression of 43 genes. The starch synthesis-related genes (SSRGs), starch synthase IV-1 (SSIV-1), starch branching enzyme 1 (SBE1), granule-bound starch synthase 2 (GBSS2), and ADP-glucose pyrophosphorylase small subunit 2a (OsAGPS2a) were found to have eQTLs regulating the expression of ECQ associated transcripts. Further, in co-expression analysis, 130 genes produced at least one network with 22 master regulators. In addition, we developed CRISPR/Cas9-edited glbl mutant lines that confirmed the role of alpha-globulin (glbl) in starch synthesis to validate the co-expression analysis. This study provided novel insights into the genetic regulation of ECQ traits, and transcripts associated with these traits were discovered that could be used in further rice breeding.

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The Effects of Transformational Leadership and Transactional Leadership on Innovative Behavior among Public Servants: The Mediating Effects of Organizational Commitment and Moderated Mediating Effects of Public Service Satisfaction (공무원의 변혁적 및 거래적 리더십이 혁신행동에 미치는 영향: 조직몰입의 매개효과 및 공직만족에 의해 조절된 매개효과)

  • Minho Jung;Jiyoung Han;Jiwon Park
    • Journal of Practical Engineering Education
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    • v.15 no.2
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    • pp.243-258
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    • 2023
  • The purpose of this study is to examine the mediating effect of organizational commitment in the relationship between transformational and transactional leadership and innovative behavior and demonstrate the moderated mediating effect of job satisfaction. To this end, 4,133 cases from the 『2021 Public Servant Life Survey』 conducted to general public servants belonging to central administrative agencies and metropolitan governments by the Korea Institute of Public Administration were used for the analysis, and SPSS 21.0 and Mplus 8.4 programs were used to test the research hypotheses. As a result of the analysis, it was confirmed that transformational and transactional leadership had a positive effect on organizational commitment and innovative behavior, and organizational commitment had a positive effect on innovative behavior. In addition, it was confirmed to have a significant mediating effect in the relationship between transformational and transactional leadership and innovative behavior. Finally, it was confirmed that the mediating effect of transformational and transactional leadership on innovative behavior through organizational commitment was moderated by the level of job satisfaction, and all the proposed hypotheses were adopted. Based on theses findings, theoretical and practical suggestions and future research suggestions were discussed.

Development of an Optimized Class Space and Map based on the Metaverse ZEP Platform (메타버스 ZEP 플랫폼 기반의 최적화된 수업 공간 및 맵 개발)

  • Ae-ran Park;Myung-suk Lee
    • Journal of Practical Engineering Education
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    • v.15 no.2
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    • pp.439 -447
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    • 2023
  • This paper aims to develop a map for optimized class space using ZEP among the metaverse platforms. As a research method, the classroom space was organized so that the subject of learning became a learner, and the classroom space was modified and supplemented to optimize while being applied to elementary school computer classes. The contents of the study investigated learners' prior perception of metaverse, and compared and analyzed the advantages and disadvantages of the metaverse platform. In addition, the map was designed by reflecting the results of the survey, and after applying the map to the class, necessary APIs and apps were installed to supplement it. As a result, the learner became the subject of learning in the metaverse space, freely identified the space, and actively participated in the class. In particular, we found that students who were passive offline and those who had a low participation rate due to lack of skills participated more actively. In particular, students who were passive offline or whose participation was low due to lack of skills participated more actively. If API and JavaScript programs are added to collect log data of learners for learning analysis, real-time feedback is possible for learners, and learner feedback is possible for instructors with statistical data. If this is possible, the metaverse space can fully expect the role of a learning assistant for learners and a teaching assistant for instructors.

Development of Deep Learning Based Ensemble Land Cover Segmentation Algorithm Using Drone Aerial Images (드론 항공영상을 이용한 딥러닝 기반 앙상블 토지 피복 분할 알고리즘 개발)

  • Hae-Gwang Park;Seung-Ki Baek;Seung Hyun Jeong
    • Korean Journal of Remote Sensing
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    • v.40 no.1
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    • pp.71-80
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    • 2024
  • In this study, a proposed ensemble learning technique aims to enhance the semantic segmentation performance of images captured by Unmanned Aerial Vehicles (UAVs). With the increasing use of UAVs in fields such as urban planning, there has been active development of techniques utilizing deep learning segmentation methods for land cover segmentation. The study suggests a method that utilizes prominent segmentation models, namely U-Net, DeepLabV3, and Fully Convolutional Network (FCN), to improve segmentation prediction performance. The proposed approach integrates training loss, validation accuracy, and class score of the three segmentation models to enhance overall prediction performance. The method was applied and evaluated on a land cover segmentation problem involving seven classes: buildings,roads, parking lots, fields, trees, empty spaces, and areas with unspecified labels, using images captured by UAVs. The performance of the ensemble model was evaluated by mean Intersection over Union (mIoU), and the results of comparing the proposed ensemble model with the three existing segmentation methods showed that mIoU performance was improved. Consequently, the study confirms that the proposed technique can enhance the performance of semantic segmentation models.

Evaluation of the Characteristics of High-Flux Reverse Osmosis Membranes with Various Additives (다양한 첨가제에 따른 고투과성 역삼투막의 특성평가)

  • Hyun Woong Kwon;Kwang Seop Im;Gede Herry Arum Wijaya;Seong Min Han;Seong Heon Kim;Jun Ho Park;Dong Jun Lee;Sang Min Eom;Sang Yong Nam
    • Membrane Journal
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    • v.33 no.6
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    • pp.427-438
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    • 2023
  • In this study, in order to improve the performance of the reverse osmosis membrane with high water flux and high salt rejection, a study was conducted on the evaluation of characteristics according to the curing temperature and time during various additives and interfacial polymerization. The morphology of the membrane with no additives and the membrane with additives both showed a "rigid-and-valley" structure, confirming that the polyamide layer was successfully polymerized on the surface of the porous support layer. In addition, the additive of 2-Ethyl-1,3-hexanediol (EHD) had improved hydrophilicity and water flux, which was confirmed by measuring the contact angle. Finally, a highly permeable TFC membrane with NaCl and MgSO4 salt rejection of 97.78% and 98.7% and a high water flux of 3.31 L/(m2⋅h⋅bar) was prepared.

TheAssessment of risk of bias in randomized controlled trials published in the Korean Journal of Physical Therapy: A 2018~2022 review (한국 물리치료 학술지에 무작위대조연구의 비뚤림 위험 평가: 2018~2022년 검토)

  • Jae Hyun Lim;Chi Bok Park;Byeong Geun Kim
    • Journal of Korean Physical Therapy Science
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    • v.30 no.4
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    • pp.82-91
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    • 2023
  • Background: Randomized controlled trials (RCTs) provide evidence on the effectiveness and safety of interventions and inform systematic reviews and guideline preparation for clinical application. However, methodological flaws can occur in many RCTs, and Cochrane's risk of bias version 2 (RoB2) can be used to evaluate RCTs' risk of bias (RoB). However, physical therapy RCTs in Korea did not confirm RoB. Therefore, the purpose of this study was to evaluate RoB using RoB2 in RCTs published in the Korean Physical Therapy Journal. Design: Review. Methods: The RCTs subject to evaluation were RCTs published in 11 physical therapy journals in Korea from 2018 to 2022. RoB2 evaluated a total of five domains: bias arising from the randomization process, bias due to deviations from intended interventions, bias due to missing outcome data, bias in measurement of the outcome, and bias in selection of the reported result. Results: A total of 616 RCTs were evaluated. As for bias arising from the randomization process, high risk was the highest at 555 (90.1%), followed by low risk at 41 (6.7%) and some concerns at 20 (3.2%). For bias due to deviations from intended interventions, the proportion of some concerns was the highest at 390 (63.3%), followed by high risk at 218 (35.4%) and low risk at 8 (1.3%). As for the bias due to missing outcome data, the rate of low risk was the highest at 399 (64.8%), followed by high risk at 159 (25.8%) and some concerns at 58 (9.4%). As for bias in measurement of the outcome, high risk was the highest at 294 (47.7%), followed by low risk at 224 (36.4%) and some concerns at 98 (15.9%). In the bias due to missing outcome data, the ratio of high risk was the highest at 610 (99%), followed by low risk at 4 (0.7%) and some concerns at 2 (0.3%). Conclusion: Most of the RoB evaluation results of RCTs published in the Korean Physical Therapy Journal were rated as high risk. Methodological quality of RCTs needs to be improved.

Mental Representation for Family in Abused Children: Focusing on Types of Child Abuse (피학대 아동의 가족에 대한 정신적 표상: 성, 연령 및 학대유형별 비교를 중심으로)

  • Kyung-Sook Lee;Jin-Ah Park;Eun Jeong Oh
    • Korean Journal of Culture and Social Issue
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    • v.14 no.3
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    • pp.1-19
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    • 2008
  • The purpose of this study is to examine the family perception according to abused children's sex, age, and abusive type. The subjects were 45 abused children including 15 physically abused, 15 neglected, and 15 physically and emotionally abused children from age 7 to 14. The results were that boys showed more noncomplient behaviors to their parents, more concerns and more rejective behaviors to examination. Girls perceived their mothers as stressors more than those of boys. School-aged children perceived their mothers as allies than those of adolescents. Adolescents showed more rejective behaviors to examination and more anger. Physically and emotionally abused children experienced more family conflicts and showed more negative resolutions, and more noncomplient behaviors than those of abused children. Neglected children showed more depressive mood than those of abused children.

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Educational achievement in Korean society (II): Psychological analysis of academic success of Korean adolescents (한국 사회와 교육적 성취 (II): 한국 청소년의 학업성취에 대한 심리적 토대 분석)

  • Uichol Kim;Youngshin Park
    • Korean Journal of Culture and Social Issue
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    • v.14 no.1_spc
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    • pp.63-109
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    • 2008
  • This article examines psychological factors that contribute to educational achievement of Korean adolescents. By reviewing empirical research, three core areas are outlined. First, positive and negative roles that Korean society play on academic achievement are analyzed. Compared to other countries, Korean society places a high premium, pressure an investment on educational achievement. This has contributed to the rapid economic growth and development, but at the same time it has created numerous social problems. Second, psychological and relational dynamics of investing in and achieving success in education are delineated. Through indigenous psychological analysis, the role parents play in motivating and regulating their children to succeed academically is reviewed. In addition, the role of teachers and friends and the coordinated efforts of Korean society are outlined. Third, future directions and transformations in education that are needed in Korean society are discussed. Although Korean adolescents are high achievers in high school, this is not the case at the university level. Since Korean students are interested in entering a prestigious university, they have developed skills in doing well in standardized tests. Educational reforms need to take into consideration individuals' personal interests, skills and creativity to ensure that the knowledge that they acquired can be used to further their career and improve their subjective well-being. Educational transformation does not imply simply adopting Western models, but developing indigenous models that can maximize human and cultural potential and stimulate curiosity, diversity and creativity that are necessary in the global era.

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Improved pregnancy rate and sex ratio in fresh/frozen in vivo derived embryo transfer of Hanwoo (Bos taurus coreanae) cows

  • Jihyun Park;Wonyou Lee;Islam M. Saadeldin;Seonggyu Bang;Sanghoon Lee;Junkoo Yi;Jongki Cho
    • Journal of Animal Science and Technology
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    • v.65 no.4
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    • pp.779-791
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    • 2023
  • This study aimed to assess the effects of embryonic developmental stage, quality grade, and fresh or frozen/thawed conditions on the pregnancy rate and sex ratio of live offspring in Hanwoo (Bos taurus coreanae) cows. The quality and developmental stage of in vivo-derived (IVD) transferred embryos were evaluated using the standard criteria of the International Embryo Technology Society. The recipient cows were synchronized using conventional (estradiol benzoate and progesterone) protocols before embryo transfer. Embryos were transferred to 297 cows, and pregnancy was monitored for 60-70 days after embryo transfer. The pregnancy rates of fresh and frozen/thawed embryos were 56.90% and 52.49%, respectively. Pregnancy rates varied according to embryo quality (56.18% for grade 1 vs. 36.67% for grade 2). Pregnancy rates also varied by developmental stage and cryopreservation (67.86% vs. 63.49% for stage 4-1, 64.00% vs. 54.72% for 5-1, and 50.00% vs. 47.83% for 6-1, in fresh embryos vs. frozen/thawed embryos, respectively). For stage 7-1, the pregnancy rates were 72.73% for fresh embryos and 20.00% for frozen/thawed embryos. In 66 fresh embryos, the sex ratio of live offspring was 5:5, whereas it was 4(female):6(male) for frozen/thawed embryos among the 95 frozen/thawed embryos. The miscarriage rate was approximately 3% higher for frozen/thawed embryos than for fresh embryos (18.1% for fresh vs. 21.1% for frozen). Seasonal fertility rates were 33.3% in spring, 55.67% in summer, 52.8% in autumn, 60.0% in winter. The following male-to-female ratios were observed in different seasons: 6.7:3.3 in spring, 4.0:6.0 in summer, 5.5:4.5 in autumn, and 3.3:6.7 in winter. The current data revealed no significant differences in pregnancy rates between fresh and frozen/thawed IVD embryos. However, there was a lower pregnancy rate with advanced-stage frozen/thawed embryos (stage 7-1). The current study provides comprehensive results for the better optimization of embryo transfer in Hanwoo cattle to obtain the desired fertility rate, pregnancy rate, and sex ratio of calves. These results provide important insights into the factors that influence the viability and success of IVD embryo transfer in Hanwoo cows and may have practical applications for improving breeding programs and reducing production costs.

The Mediating Effects of Self-Control on the Relationship between Academic Stress and Impulse Buying Tendency of Nursing University Student (간호대학생의 학업스트레스와 충동구매 경향성 간의 관계에 대한 자기통제력의 매개효과)

  • Ja-Sook Kim;A-Young Park;Jong-Hyuck Kim
    • Journal of Industrial Convergence
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    • v.21 no.10
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    • pp.81-89
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    • 2023
  • The aim of this study was to explain the mediating effects of self-control in the relationship between academic stress and impulse buying tendency. Participants were recruited from one nursing university student located in Gwangju and J city. Data were collected from 145 nursing university student. The SPSS WIN 23.0 version was used with Sobel test and Baron and Kenny's mediating effect. It was investigated whether there was a mediating effect of self-control on academic stress and impulse buying tendency. First, academic stress showed a significant effect on impulse buying tendency. Second, Self-control had a partial mediating effect in the relationship between academic stress and impulse buying tendency(z=-2.69, p<.001). To reduce the impulse buying tendency among nursing university student, it is necessary to develop education·non-education interventions that promotion self-control in addition to decreasing academic stress.