• Asian-Australasian Journal of Animal Sciences
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• 제11권1호
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• pp.43-50
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• 1998

Three hundred and fifty-two growing pigs were utilized in three growth trials to investigate the effects of crystalline amino acid supplementation on growing pigs' growth performance and feed consumption. In Exp. 1, diets were formulated on the basis of digestible amino acids. Utilization of rapeseed meal plus cottonseed meal (R + C) in diet supported similar average daily gain (ADG), feed/gain (F/G) and cost per gain (p > 0.05) to group of soybean meal (SBM). In Exp. 2, 14% CP diets containing equal amount of cottonseed meal and rapeseed meal were formulated with the addition of threonine and/or tryptophan. Supplementation with threonine improved ADG and F/G (p < 0.05). Supplementation with only tryptophan made no benefits for ADG of growing pigs. In Exp. 3, diets with different threonine level were formulated. Increasing dietary threonine to 0.54% improved (p < 0.05) ADG and F/G compared to 0.45% dietary threonine. Increase in dietary threonine did not improve ADG (p > 0.05) but decreased F/G (p < 0.05) because of a decrease in average daily feed intake. Pigs fed diet with dietary threonine/lysine ratio of 67.5% supported optimum growth performance.

• BMB Reports
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• 제43권12호
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• pp.824-829
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• 2010

Melanin synthesis is regulated by melanocyte specific enzymes and related transcription factors. $\beta$-carboline alkaloids including harmaline and harmalol are widely distributed in the environment including several plant families and alcoholic beverages. Presently, melanin content and tyrosinase activity were increased in melanoma cells by harmaline and harmalol in concentration- and time-dependent manners. Increased protein levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), and TRP-2 were also evident. In addition, immunofluorescence and Western blot analyses revealed harmaline and harmalol increased cAMP response element binding protein phosphorylation and microphthalmia-associated transcription factor expression. In addition to studying the signaling that leads to melanogenesis, roles of the p38 MAPK pathways by the harmaline and harmalol were investigated. Harmaline and harmalol induced time-dependent phosphorylation of p38 MAPK. Harmaline and harmalol stimulated melanin synthesis and tyrosinase activity, as well as expression of tyrosinase and TRP-1 and TRP-2 indicating that these harmaline and harmalol induce melanogenesis through p38 MAPK signaling.

• Molecules and Cells
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• 제21권2호
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• pp.213-217
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• 2006

Although AIMP1 (previously known as p43) is one of three auxiliary proteins bound to a macromolecular aminoacyl tRNA complex, it is also secreted as a cytokine controlling both angiogenesis and immune responses. Here we show that systemically administered purified recombinant human AIMP1 had anti-tumor activity in mouse xenograft models. In Meth A-bearing Balb/c mice, tumor volume increased about 28 fold in the vehicle treatment group, while an increase of about 16.7 fold was observed in the AIMP1-treated group. We also evaluated the anti-tumor activity of AIMP1 in combination with a sub-clinical dose of the cytotoxic anti-tumor drug, paclitaxel. The growth of NUGC-3 human stomach cancer cells was suppressed by 84% and 94% by the combinations of 5 mg/kg paclitaxel + 25 mg/kg AIMP1 (p = 0.03), and 5 mg/kg paclitaxel + 50 mg/kg AIMP1 (p = 0.02), respectively, while 5 mg/kg paclitaxel alone suppressed growth by only 54% (p = 0.02). A similar cooperative effect of AIMP1 and paclitaxel was observed in a lung cancer xenograft model. These results suggest that AIMP1 may be useful as a novel anti-tumor agent.

• 한국표면공학회지
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• 제43권2호
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• pp.111-120
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• 2010

In this study, the effects of multi-complex agents addition on characteristics of electroless Ni plating solution are investigated. The species and the concentration of complexing agents are major factors to control the deposition rate, P concentration, and surface morphology of plating films. Adipic acid increases the deposition rate in regardless of single- or mutli-complex agent addition. However, lactic acid effectively increases the deposition rate in case of multi-addition as the complex agents with adipic or sodium succinate acid. In addition, sodium citric acid and malic acid show good stabilizing effects of plating solution and lowering the deposition rate, because they have high complexibility. Therefore, it is suggested that the development of Ni-P plating solution suitable for diverse usages can be carried out systematically using the database from this study.

• 임상간호연구
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• 제16권2호
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• pp.43-55
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• 2010

Purpose: This study was done to evaluate the effects of program promoting self-efficacy patients with epicondylitis. Methods: Forty-three patients with unilateral epicondylitis were enrolled in this study between January, 2 and April, 28, 2009. The patients were assigned to one of two groups: Nineteen patients in the intervention group were given the self-efficacy promotion program with specific home instruction and the 24 in the control group were treated with conventional treatment. Pain by numeric rating scale, anxiety, and the self-efficacy of exercise were evaluated before the program, and 4 weeks after completing the intervention. The number of exercises was checked after the intervention. The data were analyzed using descriptive statistics, t-test, Chi-square test, paired t-test, Pearson's correlation coefficients with SPSS 14.0 program. Results: Anxiety in the intervention group was significantly lower than the control group(p=.018). Maximal and resting pain in the control group were lower than the intervention group( p=.000, p=.003). The self-efficacy of exercise and the number of exercises increased in the intervention group but there was no significant difference (p=.057, p=.052). Conclusion: The results of this study indicate that a program promoting self-efficacy for patients with epicondylitis could be a useful nursing intervention to reduce anxiety.

• 한국보건간호학회지
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• 제37권1호
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• pp.43-53
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• 2023

Purpose: This study aims to identify the factors influencing the depression of teachers in child day care centers. Methods: The study 181 teachers working at national/public or child day care centers certified by Seoul Metropolitan government in Seoul, South Korea. Data from September to October 2021, and emotional labor, job stress, ego resilience, stress resilience, and depression. Data were analyzed using t-test, ANOVA, Pearson's correlation coefficients, and stepwise multiple regression. Results: The average depression of child day care center's teachers 8.52±7.58 out of 60 points, and the depression group (above 21 points) was 9.4%. Depression in child day care center's teachers was positively correlated with job stress (r=.42, p<.001) and negatively correlated with ego resilience and stress resilience (r=-.55, p<.001). Factors affecting depression of child day care center's teachers were found to be job stress (p=0.005), ego resilience (p<.001), stress resilience (p=0.031) and the explanatory power of these variables was 39%. Conclusion: Results of this study indicate that in the future development of depression intervention programs for teachers of child day care centers, it is necessary to include measures that reduce job stress and strengthen ego resilience and stress resilience.

• Asian Pacific Journal of Cancer Prevention
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• 제15권7호
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• pp.2987-2991
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• 2014

Connexin 43 is an important gap junction protein in vertebrates and is known for its tumor suppressive properties. Cx43 is abundantly expressed in the human intestinal epithelial cells and muscularis mucosae. To explore the role of Cx43 in the genesis of human colon cancer, we performed the expression analysis of Cx43 in 80 cases of histopathologically confirmed and clinically diagnosed human colon cancer samples and adjacent control tissue and assessed correlations with clinicopathological variables. Western blotting using anti-Cx43 antibody indicated that the expression of Cx43 was significantly down regulated (75%) in the cancer samples as compared to the adjacent control samples. Moreover, immunohistochemical analysis of the tissue samples confirmed the down regulation of the Cx43 in the intestinal epithelial cells. Cx43 down regulation showed significant association (p<0.05) with the histological type and tumor invasion properties of the cancer. Our data demonstrated that loss of Cx43 may be an important event in colon carcinogenesis and tumor progression, providing significant insights about the tumor suppressive properties of the Cx43 and its potential as a diagnostic marker for colon cancer.

• Journal of Korean Biological Nursing Science
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• 제18권1호
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• pp.43-50
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• 2016

Purpose: This study aimed to identify the relationship among knowledge, attitudes and prevention behaviors (PB) on tuberculosis (Tb) infection in nursing students. Methods: 268 subjects were recruited from two universities located in C D cities of Korea and data were collected utilizing self-reported questionnaires. Results: The mean scores of knowledge, attitudes and PB on Tb infection were 64.83, 3.18 and 2.97. The knowledge differed according to gender (t=-3.16, p=.002), grades (F=32.19, p<.001), educational experience about Tb (EETb) (F=10.59, p<.001), learning information about Tb (t=3.08, p=.002) and getting Tb: self or others (t=2.78, p=.006). The attitudes differed according to grades (F=7.71, p<.001) and EETb (F=2.68, p=.047). The PB differed according to grades (F=7.02, p<.001) and EETb (F=4.55, p=.004). Significant correlations were found between knowledge and PB (r=.20, p=001), attitudes and PB (r=.33, p<.001). The most significant factor influencing PB was attitudes with R2 value of 13.9% (F=11.81, p<.001). Conclusion: These findings indicate that knowledge and attitude adjustment may be necessary to improve PB for Tb infection in nursing students. Moreover further study is necessary to find out the ways to reinforce the level of attitudes. The results of the study can be utilized in educational programs for preventing Tb infection in nursing students.

• 한국산학기술학회논문지
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• 제14권2호
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• pp.759-767
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• 2013

이 연구는 임상실습을 경험한 응급구조과 학생들을 대상으로 임상실습 만족도를 조사하였다. 연구결과는 실습만족도 평균 3.61(${\pm}0.70$)이었으며, 일반적 특성에 따른 실습만족도에 유의한 항목은 희망취업분야(F=3.98, p=0.004)이었다. 실습실태에 따른 실습만족도에 유의한 항목은 실습시작학년(t=0.43, p=0.038), 실습기간(F=2.82, p=0.025), 담당부서(F=11.81, p=0.000)이었다. 임상실습 술기와 실습만족도와의 상관관계에서는 관찰횟수(r=0.221, p=0.000), 시행횟수(r=0.170, p=0.002)는 실습만족도와의 유의한 양의 상관관계가 있었다. 이상의 연구결과로 응급구조과 학생들의 임상실습 만족도를 향상시키기 위해서는 확고한 직업관을 가져야 할 것이며, 임상실습 시작학년과 실습기간을 응급구조과 학생의 특성에 맞게 고려해야 할 것이다. 또한 실습기관에서 실습을 담당하는 부서 역시 응급구조과라는 특수성을 고려해야 할 것이며, 실습만족도를 높이기 위한 실습프로그램 개발 연구가 필요할 것이다.

• 한국미생물·생명공학회지
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• 제32권3호
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• pp.206-211
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• 2004

Pseudomonas aurantiaca 유래 levansucrase 유전자(lscA)를 GAL1 promoter 하류에 연결시킨 pYES-lscA와 CAL10 promoter와 Kluyveromyces marxianus exoinulinase의 분비 신호서열(INU1 ss)하류에 연결시킨 pYInu-lscA를 각각 구축하였다. 이들 plasmid를 invertase 결손 변이주(suc2-$\Delta$9)인 S. cerevisiae SEY2102에 형질전환시켜 고활성 형질전환주를 선발하였다. 효모 형질전환주를 galactose 함유 배지로 배양한 결과, pYES-lscA 함유 형질전환주인 경우 levansucrase의 총활성은 8.62 U/ml이고, pYInu-lscA 함유 형질전환주인 경우 5.43 U/ml에 도달하였다. 발현된 levansucrase의 약 80% 정도가 periplasmic space와 cytopla느에 존재하였고, INU1 ss에 의한 분비효율 증가는 관찰할 수 없었다. 또한, 효모에서 발현된 재조합 levansucrase는 과당쇄화된 형으로 생산되는 것으로 보여진다.