• Title/Summary/Keyword: p37

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Purification and characterization of an alkaline protease produced by a xanthomonas sp. YL-37

  • Lee, Chang-Ho;Kim, Hee-Sik;Seok, Kwon-Gi;Oh, Hee-Mock;kang sang mo;Kwon, Tae-Jong;Yoon, Byung-Dae
    • Journal of Microbiology
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    • v.33 no.2
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    • pp.115-119
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    • 1995
  • The alkaline protease of Xanthomonas sp. YL-37 has been purified, and the properties of the enzyme investigated. The alkaline protease of Xanthomonas sp. YL-37 was purified form crude enzyme by ammonium sulfate fractionation, CM-cellulose ion exchange chromatography, and Sephadex G-100 gel filtration. Through the series of chromatographies, the enzyme was purified to homogenecity with specific activity of 4.23 fold higher than that of the crude broth. The molecular weight of the purified protease has been estimated to be 62 KDa on SDS-polyacrylamide gel electrophoresis. The optimal pH and temperature for alkaline protease activity were 11.0 and 50.deg.C, respectively. The enzyme was stable between pH 5.0 and 10.0 and up to 50.deg.C. Enzyme activity was lost up to 50% on heating at 70.deg.C for 30 minutes. The activity of alkaline protease was inhibited by Cu$\^$2+/, Zn$\^$2+/, Hg$\^$2+/, PMSF, and activated by Mn$\^$2+/ and Ca$\^$2+/. The $K_{m}$ value for casein as a substrate was 4.0 mg/ml.

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Enzymatic Hydrolysis of Beef Tallow (효소에 의한 우지의 가수분해 반응)

  • 김인호;박태현
    • Microbiology and Biotechnology Letters
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    • v.19 no.4
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    • pp.377-382
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    • 1991
  • Reef tallow was hydrolyzed with lipase under the conditions of liquid state and solid state. Lipase OF 360 was used for that purpose, and the lipase had the maximum activity when the olive oil was used as a substrate at pH 6 and $37^{\circ}C$. Beef tallow was dispersed by an agitator to perform a liquid enzymatic reaction. Water content, reaction temperature, and enzyme amount were varied as parameters affecting hydrolysis percentage. Ninety three percents of tallow were hydrolyzed at the following conditions: water content 80% w/w, temperature $37^{\circ}C$, and enzyme amount 200 unitlg tallow. In order to conduct a solid phase enzymatic reaction, sonication was employed for pretreating tallow with the enzyme solution. Molten tallow was sonified with the enzyme solution, and solidified by lowering temperature. And then hydrolysis reaction proceeded at $30^{\circ}C$. Sonication intensity and time were varied to control hydrolysis percentage. Optimum values of the intensity and the time were found to exist since the hydrolysis percentage did not increase further according to the increases of the intensity and the time.

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Soil Microorganism Degrading Polycaprolactone (Polycaprolactone을 분해하는 토양미생물)

  • Kim Mal-Nam
    • Korean Journal of Environmental Biology
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    • v.22 no.3
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    • pp.400-404
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    • 2004
  • Polycaprolactone (PCL), a synthetic aliphatic polyester, was buried in activated sludge soil for 66 days at $27^\circ{C}$ and $37^\circ{C}$. The morphology of the surface of PCL film degraded by soil microorganisms was observed. Soil microorganisms degrading PCL were isolated and identified. Soil fungi and soil bacteria utilizing PCL as carbon or energy source were identified as Paecilomyces fumosoroseus KH27, Penicillium digitatum KH28, Fusarium solani KH29, Aspergillus sp. KH30 and Ochrobactrum anthropi KH31, respectively. Biodegradation test of PCL by the isolated strains showed that, P. digitatum KH28 exhibited the most PCL degrading activity at $27^\circ{C}$. However, at $37^\circ{C}$ O. anthropi KH31 showed higher degrading activity than the other soil microorganisms tested.

ON SUFFICIENT CONDITIONS FOR MULTIVALENT STARLIKENESS

  • Yang, Dinggong
    • Bulletin of the Korean Mathematical Society
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    • v.37 no.4
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    • pp.659-668
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    • 2000
  • Let's $S_n(p,\alpha)(p,n\in\ N={1,2,3,\cdots},0\leq\ \alpha<1)$ denote tje c;ass pf fimctopms $f(z)=z^p+a_{p+z}z^{p+n}+\cdots$ which are $\rho$-valently starlike of order $\alpha$ in the unit disk.Some criteria for a function f(z) to be in the class $S_n(\rho,\alpha)$ are given.

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인공산성비 처리에 대한 은행나무와 곰솔의 생장반응

  • 소웅영
    • Journal of Plant Biology
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    • v.37 no.1
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    • pp.93-99
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    • 1994
  • 은행나무와 곰솔의 유식물의 산성비에 대한 생장에 미치는 영향을 알아보기 위하여 pH 5.6을 대조구로 하여 pH 4.0, 3.2와 2.4의 인공산성비를 3개월간 처리하여 생장량, 엽록소 함량과 탄수화물의 함량을 조사하였다. 은행나무와 곰솔의 유식물은 pH2.4에서 생장이 가장 억제되었으며 반면에 pH 3.2에서는 생장이 촉진되었다. 전체적인 생장량은 뿌리에 비해 지상부의 생장에서 산성비의 영향을 더 받았다. 엽록소 함량은 산성비의 산도에 따라 중요한 차이가 없었으며 은행나무 잎의 탄수화물의 함량은 포도당과 설탕의 함량이 pH 4.0에서 가장 낮았고 전분의 함량은 pH 3.2에서 가장 낮았다.

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Mechanism of Peroxide-supported Hydroxylation by Cytochrome P-450 : Its Formation Pattern of the Active Intermediate (Hydroperoxide 의존성 반응에서의 Cytochrome P-450의 산화활성종 형성양식)

  • 문전옥;김기헌
    • YAKHAK HOEJI
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    • v.37 no.1
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    • pp.95-99
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    • 1993
  • Peroxidase activity of cytochrome P-450 was examined using N, N-dimethylaniline (NDA) as a substrate and cumene hydroperoxide (CHP) as an oxidant. The initial rates of the N-demethylation for varied concentrations of NDA (0.05-0.5 mM) by P-450 at different fixed concentrations of CHP (0.02-0.2 mM) were determined. The results suggest that P-450 proceeds its peroxidative reaction by the rapid equilibrium random bi bi mechanism to form a ternary complex with substrate and oxidant as an active intermediate.

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Phylogenetic Analysis, Morphology and Pathogenicity of Penicillium spp. associated with Blue Mold of Apple in Korea (사과푸른곰팡이병에 관여하는 Penicillium의 계통분석, 형태 및 병원성)

  • Sang, Hyun-Kyu;Choi, Young-Phil;Yu, Seung-Hun
    • Korean Journal of Agricultural Science
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    • v.37 no.3
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    • pp.341-350
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    • 2010
  • Blue mold is the most important postharvest disease of apples in Korea. Apple fruits with blue mold symptoms were collected from storages in different locations in Korea and were investigated for their association with Penicillium species. A total of sixty five isolates of Penicillium were sampled from the collected apples. Based on DNA sequence analysis of ${\beta}$-tublin gene and ITS and lsu rDNA (ID region) and morphological characteristics, they were identified as P. crustosum, P. expansum, P. italicum, P. solitum and P. sp.. P. sp. which is closely related to P. hirsutum is a new species, not reported before. P. expansum (35%) was predominant species followed by P. crustosum. The phylogenetic tree inferred from combined ${\beta}$-tublin and ID region sequence showed good correlation with species that are defined by morphological characteristics. In pathogenicity test, apples were wound-inoculated with conidial suspension and incubated at $20-22^{\circ}C$. The most severe and destructive species was P. expansum. The species caused a decayed area 42-50mm in diameter after 8-10days. Decayed area caused by P. crustosum and P. sp. was 26-32mm and 20-26mm, respectively. This is the first record of P. crustosum, P. italicum and P. sp. from apple in Korea.

Changes of Frozen-Thawed Semen Characteristics in Miniature Pig and Duroc (Miniature Pig와 Duroc 종간의 동결-융해 후 정액 성상 비교)

  • Lee, Y.S.;Choi, W.C.;Lee, S.H.;Cheong, H.T.;Lee, S.Y.;Yang, B.K.;Park, C.K.
    • Journal of Embryo Transfer
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    • v.21 no.3
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    • pp.263-271
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    • 2006
  • The purpose of this study was undertaken to compare ability of frozen-thawed sperm characteristics between two strains (miniature pig and Duroc). The semen was collected by gloved-hand method into a pre-warmed ($37^{\circ}C$) thermos bottle. The semen was diluted with same volume extender and added to LEY solution for freezing. The diluted semen was placed in 0.5 ml straws, and freezing was initiated by exposing the straws to liquid nitrogen ($LN_2$) vapours for 10 min before placing them into $LN_2$ for cryopreservation. The frozen-semen straw were thawed at 20, 37 and $50^{\circ}C$ for 1 min, 45 sec and 10 sec within water-bath. The semen sample were evaluated at 0, 3, 6, 9, and 12 h after incubation at $37^{\circ}C$ for analysis of sperm ability. Abnormality of spermatozoa in miniature pig was significantly (p<0.05) higher than that in Duroc at 0, 9 and 12 h of post-thawing incubation after frozen-thawing. The percentage of F-patterned spermatozoa in miniature pig was significantly (p<0.05) lower, while the percentage of AR (acrosome reacted spermatozoa) pattern was higher in the miniature than in the Duroc. On the other hand, there was no significant difference in the viability of spermatozoa thawed at different temperature ($20^{\circ}C\;and\;37^{\circ}C$) between two species, but the viability in miniature pig was higher (p<0.05) than in Duroc when sperm was thawed at $50^{\circ}C$. In conclusion, this study suggest that suitable freezing method for miniature pig semen is required for increasing post-thawing viability and fertilization capacity.

Research on the Bacillus-fermented Enzamin Administration on Human NK Cell Activity and Bone Density of Hamster: A Pilot Study (Bacillus 발효액 Enzamin의 투여가 인체의 NK세포 활성도와 Hamster의 골밀도에 미치는 탐색연구)

  • Won, Ryu Seo;Fujii, Tokio;Lee, Hyung H.
    • Journal of Naturopathy
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    • v.7 no.2
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    • pp.63-69
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    • 2018
  • Purpose: The purpose of this study was to investigate the effects of Bacillus fermentation broth (ENM) on the human NK cell activity, and bone matrix density, matrix content and area of hamsters fed ENM. Methods: NK cell activity was tested, and bone mineral density were measured by x-ray. Results: NK cell activity was significantly higher in the control group (644.71 pg/ml) and in the test group (1796.37 pg/ml) (p<.004). Test groups were significantly increased by 1110.37 pg/ml in the pre-test and 1796.37 pg/ml in the post-test(p<.001). Behavioral observations after feeding ENM to hamsters showed normal behavior with no difference between control and test groups. The hamster body weight of the control was 106 g at the initial and final 27 days. In the test group, the initial weight was 96.6 g and the final 27 days was 114 g. No morphological changes were observed in the X-ray photographs of the hamster hind legs. The bone matrix density was 0.059 g/cm3 in control and 0.062 g/cm3 in the test, which was increased by 0.003 g/cm3. The bone matrix content was 0.175 g in the control and 0.196 g in the test. The bone area was 2.95 cm2 in the control and 3.19 cm2 in the test, which was increased by 0.19 cm2. In the autopsy, neither the control nor the test group showed any remarkable abnormality, and each organ was normal. Conclusions: It is thought that ingestion of ENM is useful for immunity enhancement.

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Genetic Relationship of Pleurotus ferulae Strains (아위버섯(Pleurotus ferulae) 균주의 유전적 유연관계)

  • Choi, Jae-Sun;Lee, Dong-Hee;Chang, Hu-Bong;Kang, Bo-Gu;Koo, Chang-Duck
    • The Korean Journal of Mycology
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    • v.37 no.1
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    • pp.28-32
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    • 2009
  • This study was carried out to investigate the genetic relationship of Pleurotus ferulae, an edible mushroom found on a medicinal plant, Ferula assa-foetida, in central China. The genetic relationships of 15 Pleurotus species strains, including five P. ferulae strains were analyzed. The strains were divided into seven groups at 80% genetic similarity level according to random amplified polymorphic DNA (RAPD) analysis. Four out of the seven groups consisted of two to four strains, while the other three groups consisted of three strains. In each of the three groups, the three strains were from each of three different Pleurotus species (P. cornucopiae, P. florida and P. sajorcaju). Other strains grouped together for genetic similarity were P. eryngii 26060 and P. fuscus var. ferulae 26065, three strains of P. ostreatus, and four P. ferulae strains (Bakdal, Awi, Cheonsan 1, and Yesan). However, Japanese Seolyi which belongs to P. ferulae and Heukpyung which belongs to P. ostreatus were together in a separate group.