Mechanism of Peroxide-supported Hydroxylation by Cytochrome P-450 : Its Formation Pattern of the Active Intermediate

Hydroperoxide 의존성 반응에서의 Cytochrome P-450의 산화활성종 형성양식

Peroxidase activity of cytochrome P-450 was examined using N, N-dimethylaniline (NDA) as a substrate and cumene hydroperoxide (CHP) as an oxidant. The initial rates of the N-demethylation for varied concentrations of NDA (0.05-0.5 mM) by P-450 at different fixed concentrations of CHP (0.02-0.2 mM) were determined. The results suggest that P-450 proceeds its peroxidative reaction by the rapid equilibrium random bi bi mechanism to form a ternary complex with substrate and oxidant as an active intermediate.