• 한국미생물·생명공학회지
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• 제14권3호
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• pp.213-218
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• 1986

B. amyloliquefaciens의 $\alpha$-amylase 유전자가 S. cerevisiae 내에서 형질발현하는 가를 조사하기 위하여 본, 연구에서 YRp7 plasmid에 B. amyloliquefaciens amylase유전자를 cloning하여 만든 pEA24를 형질전환시켰다. 먼저 YRp7 plasmid를 이용하여 형질전환 최적 조건을 검토하여 본 바, PH 7과 8사이, 반응온도 3$0^{\circ}C$에서 40%의 polyethylene glycol(MW 4,000)을 처리한 후 2 %의 agar를 함유한 재생배지에 중층도말 하였을 때 형질전환율이 가장 높았다. 형질전환주로부터 생성된 amylase의 활성을 측정한 결과, S. cerevisiae에서 약간의 amylase활성을 나타내어 최고 B. amyloliquefaciens의 2% 정도였고, 세포외효소는 검출되지 않았다. 이들 형질전환 주가 가지고 있는 pEA24 plasmid의 안정성을 조사한 결과 YRp7보다 불안정하였으며, 추출한 DNA를 전기영동하여 그 band를 확인하였다.

• 한국초지조사료학회지
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• 제33권3호
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• pp.197-205
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• 2013

An in vitro study was conducted to determine the effects of defaunation (removal of protozoa) and forage sources (rice straw, ryegrass and tall fescue) on ruminal fermentation characteristics, methane ($CH_4$) production and degradation by rumen microbes. Sodium lauryl sulfate, as a defaunation reagent, was added into the mixed culture solution to remove ruminal protozoa at a concentration of 0.375 mg/ml. Pure cellulose (0.64 g, Sigma, C8002) and three forage sources were incubated in the bottle of culture solution of mixed rumen microbes (faunation) or defaunation for up to 24 h. The concentration of ammonia-N was high under condition of defaunation compared to that from faunation in all incubations (p<0.001). Total VFA concentration was increased at 3, 6 and 12 h (p<0.05~p<0.01) but was decreased at 24 h incubation (p<0.001) under condition of defaunation. Defaunation decreased acetate (p<0.001) and butyrate (p<0.001) proportions at 6, 12 and 24 h incubation times, but increased propionate (p<0.001) proportion at all incubation times for forages. Effective degradability of dry matter was decreased by defaunation (p<0.001). Defaunation not only decreased total gas (p<0.001) and $CO_2$ (p<0.01~0.001) production at 12 and 24 h incubations, but reduced $CH_4$ production (p<0.001) at all incubation times for all forages. The $CH_4$ production, regardless of defaunation, in order of forage sources were rice straw > tall fescue > ryegrass > cellulose (p<0.001) up to 24 h incubation.

• 한국측량학회지
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• 제8권2호
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• pp.45-51
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• 1990

수치표고모델은 실제지형모델의 참값과 비교하여 충분하고 높은 정확도를 지녀야 하며, 미리 주어져 있는 지형데이타와 보간법을 사용하여 임의의 평면위치(X, Y)에 대응하는 표고 h를 구하고 경사를 구할 수 있는 모텔을 만들어 놓아야 한다. 보간의 정확도는 지형의 기복 상태와 자료의 밀도에 우선적으로 영향을 받게 되므로, 지형을 객관적인 방법으로 분류할 필요가 있다. 평균경사도와 면적비는 지형이 경사상태에 따라 비슷한 분류 결과를 가져오는 변수이지만, 지형의 국소적인 변화크기를 동시에 표시하는 변수는 면적비이다. 그러므로 본 연구는 지형의 분류를 좀 더 객관화하기 위하여 비고에 의해서가 아니라 경사도에 의해 분류하고, 경사와 곡률경중율의 영향을 분석하여 그 경사도에 합당한 매개변수를 도입하므로써 보다 현실에 가까운 모델을 재현하는데 연구의 목적이 있다. 연구의 결과는 첫째, 경사에 의한 지형의 분류는 평지는 pl6과 p24 준경사지 pl6과 S, 급경사지는 S 와 p24가 적합하고 곡률에 의한 분류는 평지와 준경사지에서 모두 p24와 S가 적합하며, 급경사지에서는 pl6이 적합하였다. 경사와 기복 변화량을 조합한 경우는 평지는 pl6, 준경사지는 p24, 급경사지는 S가 각각 적합하였다. 둘째, 유형화율은 경사 50%, 곡률경중율 0.0015에서 50∼80%로 가장 컸다.

• Journal of Nutrition and Health
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• 제3권2호
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• pp.101-106
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• 1970

Radioactive Phosphorus $(^{32}P)$ was administered intramuscularlly to the newly hatched chicken in the purpose of determination of the uptake and the distribution, as related to sex and hour differences of the several organs of the bodies. $2\;{\mu}\;of\;^{32}P$ was administered to each chick, and the distribution of 32P was observed in 1 hour and 24 hours after administration. In this experiment 80 heads of chicken were used(40 chicken were one day and 40 chicken were 7 days old) and the results obtained as follows: 1. The tissue showed an uptake rate of $^{32}P$ dose per 100 milligram of tissue in one day old chicken, with the following sequence: Males (1 hour): Femur. Liver. G., Muscle. Testis. Brain (24 hour): Femur, Testis, Gastrocnemius Muscle, Liver, Brain. Female(1 hour): Femur, Liver, Gastronemius Muscle, Ovary, Brain. (24 hour): Femur, Liver, Gastrocnemius Muscle, Ovary, Brain. 2. In 1 hour, the uptake rate of $^{32}P$ of the tissues showed significant difference between the male and the female except the gastrocnemius muscle and the brain in one day old group, but they were no significance except the testis and ovary after 24 hours. 3. The distribution of $^{32}P$ of the tissues exhibited higher in 1 hour than in 24 hours except the femur, the brain of the male and female, the brain and gastrocnemius muscle of the female in one day old group. 4. The tissue showed an uptake rate of $^{32}P$ dose per 100 miligram of tissue in 7days old chicken, with the following sequence: Male (1 hour): femur, liver, gastrocmenius muscle, testis, brain. (24 hour): femur, testis, gastrocmenius muscle, liver, brain. Female(1 hour): femur, liver, gastrocmenius muscle, ovary, brain. (24 hour): femur, ovary, liver, gastrocmenius muscle, brain. 5. The distribution of $^{32}P$ of the tissues showed no significant difference between the male and the female except the testis and ovary after 24 hours in 7 days old chicken group. 6. The distribution of $^{32}P$ the tissues exhibited higher in 1 hour in 24 hours except the femur, the brain of the male and the female, the brain and the ovary of the female in 7 days old chicken group.

• 호남수학학술지
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• 제34권1호
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• pp.93-101
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• 2012

Let $E_A^B$ denote the elliptic curve $E_A^B:y^2=x^3+Ax+B$. In this paper, we calculate the number of points on elliptic curves $E_A^0:y^2=x^3+Ax$ over $\mathbb{F}_p$ mod 24. For example, if $p{\equiv}1$ (mod 24) is a prime, $3t^2{\equiv}1$ (mod p) and A(-1 + 2t) is a quartic residue modulo p, then the number of points in $E_A^0:y^2=x^3+Ax$ is congruent to 0 modulo 24.

• KSBB Journal
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• 제24권1호
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• pp.106-112
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• 2009

An optical sensing membrane has been fabricated to measure the concentration of dissolved oxygen(DO) and pH value simultaneously. It has employed HPTS as a pH sensitive dye and a ruthenium(II) complex as a DO sensitive dye. The sensing membrane has been applied to wells in a 24-well microtiter plate. Using the 24-well microtiter plate the concentrations of dissolved oxygen and pH values have been on-line monitored during the cultivations of E.coli DH5${\alpha}$, B.cereus 318 and P.pastoris X-33. On-line monitoring of DO and pH in microorganism cultivation processes showed good performance of the sensing membrane containing 5 mM HPTS and 2 or 5 mg/mL Rudpp.

• Asian-Australasian Journal of Animal Sciences
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• 제12권2호
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• pp.165-168
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• 1999

To determine the effects of genetic and environmental influences on cell mediated immune (CMI) responses in broiler rabbits, contact sensitivity to 2,4-Dinitrochlorobenzene (DNCB) was assessed in three temperate broiler breeds of rabbits, namely Soviet Chinchilla, New Zealand White and Grey Giant. The feasibility of using the contact sensitivity to DNCB as a marker trait in selection for disease resistance was examined. There were highly significant differences between breeds (p<0.01) in initial skin thickness and contact sensitivities to DNCB at 24, 48 and 72 hours. Initial skin thickness was greatest in the Soviet Chinchilla breed (mean 2.2484 mm), and was significantly greater (p<0.01) in males (2.4963 mm) than in females (1.7846 mm) (p<0.01). Highest contact sensitivity to DNCB was in the New Zealand White breed with mean increase in skin thickness of 1.1884, 0.9072 and 0.5879 mm at 24, 48 and 72 hours post challenge respectively. Delayed type hypersensitivity (DTH) reaction to DNCB at 24 hours post challenge had a highly significant association (p<0.01) with the incidence of body mange in rabbits. The results indicated a lowered contact sensitivity to DNCB at 24 hours post challenge was associated significantly (p<0.01) with an increase in incidence and severity of body mange, suggesting its potential value as a marker. The correlation s among contact sensitivities at 24, 48 and 72 hours were positive and highly significant (p<0.01); correlations between initial skin thickness and contact sensitivities were negative and highly significant (p<0.01). Another notable significant correlation was between body weight and delayed type hypersensitivity at 24 hours indicating that an enhanced CMI might be associated with better growth rate and general wellbeing.

• 대한간호학회지
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• 제23권2호
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• pp.284-298
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• 1993

This study was carried out to compare perceptions about nursing activities in oriental medical hospital settings. Data were collected from 47 patients hospitalized in an oriental medical hospital, and 41 nurses and 47 oriental medical doctors working in four oriental medical hospitals from July 10th to Sept.20th, 1991. The findings of this study are as follows. 1. There were significant differences in the perceptions about physical nursing activities between patients ( M=44.07) and nurses (M=48.44) (t=-3.09. p=0.003) , and between nurses and oriental medical doctors (M=41.47) (t=-5,20. p=0.000). 2. There were no differences in perceptions about psychological and emotional nursing activities between patients ( M=27.64) and nurses (M=28.52) (t=-1.02, p=0.310), but there were differences between nurses and oriental medical doctors ( M=24. 31) (t=-5.31, p=0.000). 3. There were differences in perceptions about observation, recording and implemention of medical care activities between patients (M=53.65) and nurses (M=57.08) (t=-2.15, p=0.034), but there were no differences between nurses and oriental medical doctors (M=57.28) (t=0.14, p=0.892). 4. There were no differences in perceptions about nursing management activities between patients (M=24.88) and nurses (M=26.42) (t=-1.91, p=0.059), but there were differences between nurses and oriental medical doctors (M=24.25) (t=-3.24, p=0.002).

• 한국식품과학회지
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• 제34권1호
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• pp.91-95
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• 2002

본 연구의 목적은 동결건조된 김치를 시료로 선정하여 동결건조 시간이 건조된 김치제품의 미생물학적인 특성, 관능적 특성 및 다른 품질에 미치는 영향을 조사하여 "건조김치"의 제조에 적합한 동결건조 시간을 알아내는 것이다. 김치를 24, 48, 72시간 동결건조한 후, 건조제품의 젖산균수, pH, 관능적 특성, 파쇄도, 복원성 및 수분함량, 배추조직의 미세구조를 관찰하여 동결건조 시간이 건조김치의 품질에 미치는 영향을 조사하였다. 원시료에 비하여 24시간 건조시킨 제품의 생균수 및 pH가 현저하게 변화하였으나, 그 후에는 72시간까지 거의 변화가 없었다. 24시간 건조김치보다 48시간 또는 72시간 건조김치의 전반적인 기호성이 우수하였다. 동결 건조 시간이 24시간에서 48시간, 72시간으로 증가할수록 건조김치의 파쇄성은 증가하였다. 동결건조 시간이 경과할수록 건조김치의 복원성이 개선되고, 건조김치의 중량과 함유된 수분함량은 감소하였다. 본 실험의 조건하에서는 건조김치의 제조에 적합한 동결건조 시간은 미생물학적인 측면에서 보면 24시간도 큰 문제는 없으나, 관능적 특성과 건조제품의 품질특성의 측면에서 판단하면 48시간이 보다 바람직하였다.

• 대한의생명과학회지
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• 제29권4호
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• pp.287-295
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• 2023

Amifostine was developed to protect cells, but it is known to induce cytotoxicity and apoptosis, and the exact mechanism is unknown. In this study, we investigated how the DNA mismatch repair (MMR) system interacts with p53 to prevent apoptosis, cell cycle arrest, and cytoprotective effects induced by amifostine. HCT116 colon cancer cells sublines HCT116/p53+,HCT116/p53+, HCT116/p53-, HCT116/E6 and HCT116+ch3/E6 cells were used for evaluation. Amifostine induced G1 arrest and increased toxicity two-fold in p53- cells regardless of MMR expression. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Amifostine induced the expression of p21 protein in both p53+ and p53- cells. As for apoptosis, compared to p53- cells, p53+ cells showed 3.5~4.2 times resistance to amifostine-induced apoptosis. HCT116+E6 with both p53 and MMR loss showed maximum apoptosis at 48 h, and HCT116+ch3/E6HCT116+ch3/E6 with p53 loss showed maximum apoptosis at 24 h. As a result, it was confirmed through in vitro experiments that amifostine-induced G1 cell cycle arrest and apoptosis are mediated through a pathway dependent on MMR and p53 protein.